Search Results (9,240)

Preformed porous media (PPM) technology has emerged as a transformative approach to enhance heat and mass transfer in vacuum freeze-drying (VFD) of agricultural and food products. This review systematically analyzes recent advances in PPM research, with particular focus on spray freeze-drying (SFD) as the dominant technique for precision pore architecture control. Empirical studies confirm PPM’s efficacy: drying time reductions of 20–50% versus conventional VFD while improving product quality (e.g., 15% higher ginsenoside retention in ginseng, 90% enzyme activity preservation). Key innovations include gradient porous structures and multi-technology coupling strategies that fundamentally alter transfer mechanisms through: resistance mitigation via interconnected macropores (50–500 μm, 40–90% porosity), pseudo-convection effects enabling 30% faster vapor removal, and radiation enhancement boosting absorption by 40–60% and penetration depth 2–3 times. While inherent VFD limitations (e.g., low thermal conductivity) persist, we identify PPM-specific bottlenecks: precision regulation of pore structures (<5% size deviation), scalable fabrication of gradient architectures, synergy mechanisms in multi-field coupling (e.g., microwave-PPM interactions). The most promising advancements include 3D-printed gradient pores for customized transfer paths, intelligent monitoring-feedback systems, and multiscale modeling bridging pore-scale physics to macroscale kinetics. This review provides both a critical assessment of current progress and a forward-looking perspective to guide future research and industrial adoption of PPM-enhanced VFD. Full article

This study aimed to assess the anti-inflammatory properties of mare milk by analyzing immune markers in mice following gavage of mare milk. Metagenomic sequencing was employed to examine variations in the composition and functional profiles of the intestinal microbiota across different experimental groups. Bacterial diversity, abundance, and functional annotations of gut microbiota were evaluated for each group. The results show that, compared to the control group, the mare milk group exhibited a significant decrease in the pro-inflammatory cytokine IL-6 levels and a significant increase in secretory immunoglobulin A (SIgA) levels (p < 0.05). The fermented mare milk group and the pasteurized fermented mare milk group demonstrated a significant downregulation of the pro-inflammatory cytokines TNF-α and IL-1β, along with a significant increase in the anti-inflammatory cytokine IL-10 levels (p < 0.05). Additionally, metagenomic analysis revealed that both the mare milk and fermented mare milk groups were able to regulate the imbalance of the intestinal microenvironment by improving the diversity of the gut microbiota and reshaping its structure. Specifically, the mare milk group enhanced gut barrier function by increasing the abundance of Bacteroides acidifaciens, while the fermented mare milk group increased the proportion of Bacillota and the relative abundance of beneficial bacterial genera such as Faecalibaculum and Bifidobacterium. KEGG pathway annotation highlighted prominent functions related to carbohydrate and amino acid metabolism, followed by coenzyme and vitamin metabolism activities. In conclusion, mare milk and its fermented products demonstrate anti-inflammatory effects, particularly in modulating immune responses and inhibiting inflammatory cascades. Additionally, the administration of mare milk enhances the composition and metabolic activity of intestinal microbiota in mice, supporting intestinal microecological balance and overall gut health, and offering valuable insights for the development of mare milk-based functional foods. Full article

This study examines the evolution of agricultural raw material exports in seven emerging economies of Central and Eastern Europe (Romania, Poland, Slovakia, Croatia, Bulgaria, the Czech Republic, and Hungary) from 1995 to 2023 and provides forecasts for 2024–2026 using ARIMA models. The results indicate a general downward trend in the share of agricultural raw material exports within total exports, reflecting ongoing economic modernization and a structural shift toward higher value-added products and industrial sectors. Romania, Poland, and Hungary remain as significant players in the cereals market, while Slovakia and the Czech Republic show the most pronounced transitions toward non-agricultural industries. Croatia, however, follows an atypical trajectory, maintaining a relatively high share of agricultural exports. Statistical tests (Dickey–Fuller) confirm the non-stationarity of the initial series, necessitating differencing for ARIMA modeling. Correlation analyses reveal a synchronized regional dynamic, with strong links among Poland, Slovakia, the Czech Republic, and Bulgaria. Forecasts suggest continued decline or stabilization at low levels for most countries: Romania (0.45% in 2026), Poland (0.93%), Slovakia (0.62%), Bulgaria (0.51%), the Czech Republic (0.95%), and Hungary (0.53%), while Croatia is an exception, with a projected moderate increase to 4.19% in 2026. Although the share of raw agricultural exports is decreasing, the findings confirm that agriculture remains a strategic sector for food security and regional trade. The study recommends investments in processing, technological modernization, and export market diversification to strengthen the competitiveness and resilience of the agricultural sector in the context of global economic transformations. Full article

(1) Background: As a high-biomass cereal crop, maize provides substantial raw materials for food, animal feed, and processing industries. Plant root systems, vital for nutritional support, are directly vulnerable to diverse stressors that result in developmental abnormalities. Anthocyanins function as essential antioxidants, serving not only as natural pigments, but also playing crucial roles in plant stress resistance. As an essential plant hormone, jasmonic acid (JA) mediates plant stress adaptation and developmental processes, and is frequently employed to stimulate anthocyanin production. (2) Methods: Due to scarce reports on JA functions in maize, we specifically examined JA-triggered developmental regulation and anthocyanin biosynthesis using transcriptomic and metabolomic analysis. (3) Results: Phenotypic analyses revealed that exogenous JA application promoted culm development and intensified pigmentation, while enlarging the areas of stems and primary roots. Combined with phenotypic variations, our integrated transcriptomic and metabolomic analyses of root tissues also indicated that significantly altered metabolites specifically clustered within the flavonoid biosynthesis pathway. Moreover, GO and KEGG enrichment analyses of the associated differentially expressed genes confirmed their participation in this synthetic pathway with high confidence. These findings strongly suggest that methyl jasmonate (MeJA) exposure primarily modulates flavonoid biosynthesis, particularly through regulation of F3H and DFR gene expression, thereby enhancing flavonoid/anthocyanin accumulation in roots. Additionally, our correlation analysis of transcription factors revealed that Zm00001d018097 (MYB), Zm00001d029963 (MYB), and Zm00001d000236 (bHLH) likely participate in regulating the expression of structural genes, thereby promoting the upregulation of functional gene expression. (4) Conclusions: These results provide a robust framework for deciphering the MeJA-mediated regulation of anthocyanin biosynthesis in maize radicles, specifically demonstrating that Zm00001d018097 (MYB), Zm00001d029963 (MYB), and Zm00001d000236 (bHLH) coordinately enhance the expression of F3H and DFR. Full article

Vegetable oils structured with natural wax blends have attracted increasing interest due to their tunable crystallization and gelling behavior. This study evaluated the structuring of chia oil (ChO) using low concentrations (1–5%) of a shellac wax (SW) and beeswax (BW) blend in a 1:1 ratio, focusing on physicochemical, viscoelastic, and thixotropic properties. ChO structured with 1% SW/BW formed a weak network with high oil loss, whereas concentrations of 3–5% formed denser networks, resulting in OBC values of 75.6–88.4% and firmness values of 16.9–55.1 g. Structuring with 5% SW/BW significantly reduced peroxide values (p < 0.05), indicating a reduction in oxidative deterioration after oleogelation, while concentrations of 1–3% had no significant effect (p > 0.05). Although induction periods were slightly extended in structured samples, differences across oleogelant concentrations were not statistically significant (p > 0.05). Rheological analysis revealed that 3–5% SW/BW-structured ChO exhibited semisolid gel behavior, characterized by enhanced deformation resistance and thermal stability. Thixotropic recovery tests revealed that structural recovery improved as the deformation amplitude decreased within the linear viscoelastic range, suggesting that thixotropic behavior was influenced by oleogelant concentration. These findings demonstrate the potential of SW/BW-structured ChO as fat alternatives in lipid-based foods that require mechanical resilience, structural recovery, and enhanced oxidative stability, even at low wax levels. Full article

This study aimed to investigate the production of nanoemulgels structured with flaxseed fiber, designed to simulate salad dressings. For this purpose, the influence of microfluidizer passes (from one to four) on physicochemical and rheological properties was determined, followed by an assessment of thermal behavior. Rotor–stator homogenization followed by microfluidization were employed to produce nanoemulgels, which were characterized using laser diffraction, multiple light scattering, and rheological measurements. The resulting systems exhibited monomodal particle size distributions with mean diameters below 220 nm. Increasing the number of microfluidizer passes from one to four led to slight reductions in particle size, although they were not statistically significant. The formulation with two passes demonstrated superior physical stability during aging studies. Rheological evaluation indicated enhanced gel-like behavior with up to three passes, whereas excessive energy input (four passes) slightly compromised structural integrity. The linear viscoelastic region decreased notably after the first pass but remained relatively stable thereafter. The two-pass nanoemulgel, identified as the optimal formulation, was further tested for thermal stability. Temperature increases (5–20 °C) led to minor decreases in viscosity and firmness, yet the structure remained thermally stable. These findings support microfluidization as an effective strategy for developing stable flaxseed fiber-based nanoemulgels, with potential applications in functional food systems. Full article

In the context of global climate change and growing food security challenges, this study provides a comprehensive analysis of the yields of three staple crops (wheat, corn and rice) in the Yellow River Basin of China, employing multiple quantitative analysis methods including the Mann–Kendall trend test, center of gravity transfer model and hotspot analysis. Our research integrates yield data covering these three crops from 72 prefecture-level cities across the Yellow River Basin, during 2000 to 2023, to systematically examine the temporal variation, spatial variation and spatial agglomeration characteristics of the yields. The study uses GeoDetector to explore the impacts of natural and socioeconomic factors on changes in crop yields from both single-factor and interactive-factor perspectives. While traditional statistical methods often struggle to simultaneously handle complex causal relationships among multiple factors, particularly in effectively distinguishing between direct and indirect influence paths or accounting for the transmission effects of factors through mediating variables, this study adopts Structural Equation Modeling (SEM) to identify which factors directly affect crop yields and which exert indirect effects through other factors. This approach enables us to elucidate the path relationships and underlying mechanisms governing crop yields, thereby revealing the direct and indirect influences among multiple factors. This study conducted an analysis using Structural Equation Modeling (SEM), classifying the intensity of influence based on the absolute value of the impact factor (with >0.3 defined as “strong”, 0.1–0.3 as “moderate” and <0.1 as “weak”), and distinguishing the nature of influence by the positive or negative value (positive values indicate promotion, negative values indicate inhibition). The results show that among natural factors, temperature has a moderate promoting effect on wheat (0.21) and a moderate inhibiting effect on corn (−0.25); precipitation has a moderate inhibiting effect on wheat (−0.28) and a moderate promoting effect on rice (0.17); DEM has a strong inhibiting effect on wheat (−0.33) and corn (−0.58), and a strong promoting effect on rice (0.38); slope has a moderate inhibiting effect on wheat (−0.15) and a moderate promoting effect on corn (0.15). Among socioeconomic factors, GDP has a weak promoting effect on wheat (0.01) and a moderate inhibiting effect on rice (−0.20), while the impact of population is relatively small. In terms of indirect effects, slope indirectly inhibits wheat (−0.051, weak) and promotes corn (0.149, moderate) through its influence on temperature; DEM indirectly promotes rice (0.236, moderate) through its influence on GDP and precipitation. In terms of interaction effects, the synergy between precipitation and temperature has the highest explanatory power for wheat and rice, while the synergy between DEM and precipitation has the strongest explanatory power for corn. The study further analyzes the mechanisms of direct and indirect interactions among various factors and finds that there are significant temporal and spatial differences in crop yields in the Yellow River Basin, with natural factors playing a leading role and socioeconomic factors showing dynamic regulatory effects. These findings provide valuable insights for sustainable agricultural development and food security policy-making in the region. Full article

Cultivated land concentration and contiguity, as a core element of agricultural modernization development, holds strategic significance for enhancing agricultural production efficiency and ensuring national food security. This study employs vector patches as research units and classifies spatial connections between patches into direct and indirect connections. We quantify six types of spatial relationships between patches using binary encoding, enabling precise delineation of concentrated contiguous cultivated land. A Patch Connectivity Index is proposed. Combined with the Patch Area Index and Patch Shape Index, an evaluation system for cultivated land concentration and contiguity is established. Using Suixi County as a case study, we investigate the spatiotemporal evolution of its cultivated land concentration and contiguity from 2019 to 2023. Overall, patch connectivity exhibits a “single-element dominant, multi-element complementary” structural pattern, while the evaluation grading of cultivated land concentration and contiguity follows a normal distribution. Between 2019 and 2023, the average patch area decreased while the average number of connections between patches increased, indicating significant improvement in cultivated land concentration and contiguity levels. By adjusting spatial relationships between patches, the effective integration and utilization of cultivated land resources can provide theoretical foundations and practical references for agricultural modernization development. Full article

Glycitin is a kind of compound found in soybeans that has attracted increasing attention as a good source of nutrients due to its potential applications in medicine, cosmetics, and food. However, a comprehensive review of glycitin is lacking to help readers understand the current state of research on isoflavones. The present review summarizes recent progress made on the structures and functions of glycitin, with future perspectives to maximize their value and applications using bibliometric analysis methods. The health effects attributed to glycitin include estrogenic effects and anti-osteoporosis effects, as well as antioxidant, anti-tumor, hypolipidemic, and hypoglycemic effects. This review can not only deepen the understanding of the functions of glycitin, but also lay an important foundation for the further development and utilization of soybean resources. Full article

Selenium (Se) is an essential trace element for the human body and plays a vital role in various physiological processes. Plants serve not only as a major dietary source of selenium but also as natural biofactories capable of synthesizing a wide range of organic selenium compounds. The bioavailability and toxicity of selenium are highly dependent on its chemical form, which can exert varying effects on human physiology. Among these, organic selenium species exhibit higher bioavailability, lower toxicity, and greater structural diversity. In recent years, plant-derived selenium-containing compounds—selenium-enriched proteins, peptides, polysaccharides, polyphenols, and nanoselenium—have garnered increasing scientific attention. Through a systematic search of databases including PubMed, Web of Science, and Scopus, this review provides a comprehensive overview of selenium uptake and transformation in plants, selenium metabolism in humans, and the classification, composition, structural features, and biological activities of plant-derived selenium compounds, thereby providing a theoretical basis for future research on functional foods and nutritional interventions. Full article

Worldwide, 13.3% of food was wasted in 2020. Ethylene biosynthesis, responsible for fruit ripening, regulates key processes in plant growth and aging. Aptamers are DNA or RNA molecules with the capacity to bind with high affinity and specificity to proteins due to their three-dimensional structure. Therefore, conventional aptamer selection methods are often costly, inefficient, and time-consuming. In this context, in silico molecular docking offers an efficient alternative, enabling the evaluation of binding potential prior to experimental assays. This research identified aptamers with high predicted affinity for the 1-aminocyclopropane-1-carboxylate synthase (ACC synthase) and 1-aminocyclopropane-1-carboxylate oxidase (ACC oxidase) enzymes, essential in ethylene biosynthesis. Using ZDOCK for preliminary screening and HDOCK for refined analysis, aptamer-enzyme interactions were modeled. Aptamers AB451 and ABR6P.1 showed promising binding to ACC synthase, while RO33828 and O0O6O1 were optimal for ACC oxidase. These results represent a computational foundation for the development of aptamer-based inhibitors to potentially delay ripening and reduce postharvest losses. Experimental validation will be required to confirm their inhibitory function. Full article

Food security among college students is an increasing concern, with potential implications for their health, academic performance, and future well-being. This study investigated food security within a college community in Mexico, focusing on food availability, access (both economic and physical), and consumption patterns. A mixed-methods approach was employed at Ciudad Universitaria, BUAP, Mexico, between 2023 and 2024. Stratified random sampling was used, resulting in a final sample of 606 students. Data were collected through structured questionnaires covering sociodemographic characteristics and eating habits, the ELCSA, structured cafeteria observations, semi-structured interviews with key informants, and three focus groups. Statistical analysis was performed using chi-square tests (p < 0.05). Post hoc analysis with Bonferroni adjustment confirmed that origin (p = 0.0017), mode of transportation (p = 2.31 × 10⁻⁵) and private vehicles (p = 1.77 × 10⁻⁵) were the key determinants. Although the environment offered a variety of options, processed and ultra-processed products dominated the food choices. A total of 95.9% of students purchased food on campus, yet only 21.8% reported engaging in healthy eating habits. Focus groups revealed that students’ food choices were influenced by availability, access, and perceptions of affordability and convenience. These findings highlight the urgent need for targeted interventions to improve food security and promote healthier dietary practices within the college setting. Full article

Several decades after the development of FEM in computer-based form, which is a milestone in the evaluation of mechanical systems, the method has been adopted to analyze the biomechanical response of human skeletal structures. This innovative technique has generated new questions, but also new results, and, at the same time, competitive environments with explosive development, in the recent period. This research is focused on analyzing, using FEM, the extreme thermal variations produced at the level of two oro-facial systems (one control and one subjected to orthodontic therapy using a fixed metallic orthodontic appliance). The objective of the study was to determine the temperature evolution in different dental structures subjected to extreme temperatures given by variations between very cold and very hot foods. Each system was exposed to a succession of extreme thermal regimes (70…−18…70… °C and −18…70…−18… °C). In order to conduct this research, we used the case of a 14-year-old female patient. Following an orthodontic evaluation, we discovered that the patient had dento-alveolar disharmony with crowding. The straight-wire method of applying a fixed metallic orthodontic appliance was chosen. As complementary examinations, the patient was subjected to a bimaxillary CBCT. Using a series of programs (InVesalius, Geomagic, SolidWorks, and AnsysWorkbench), a three-dimensional model was obtained. This model contained jaws and teeth. Also, brackets, tubes, and orthodontic wires can be incorporated into the model. Following the simulations carried out in this study, it was found that thermal variations from the dental pulp are more severe for the oro-facial system with a fixed metallic orthodontic appliance (regardless of the type of thermal stimulus used). Thus, even today, with all the facilities available in the dental materials industry, metallic orthodontic devices present significant thermal conductivity, generating harmful effects on the dental structures. The reading of the results was performed on the virtual model, more precisely, on the internal dental structures (enamel, dentin, and pulp). A statistical study was not performed because it was considered that, in other patients, the results would be similar. Full article

Modulating enzymatic activity through physical strategies is increasingly recognized as a powerful approach to optimizing biocatalytic processes in food and biotechnology applications. Cellobiose 2-epimerase (C2E), a key enzyme for synthesizing epilactose, a non-digestible disaccharide with established prebiotic effects, is gaining relevance in functional foods. Emerging strategies, such as the application of moderate electric fields (MEFs), have attracted attention due to their non-thermal, non-invasive nature and their capacity to influence the structural and functional properties of proteins. This review assesses the potential of MEFs to modulate C2E activity and provides an overview of the physicochemical principles governing MEF–protein interactions and summarizes findings from various enzymatic systems, highlighting changes in activity, stability, and substrate affinity under electric field conditions. Particular attention is given to the mechanistic plausibility and processing implications of applying MEFs to C2E-catalyzed reactions. The integration of biochemical, structural, and engineering perspectives suggests that MEF-assisted modulation could overcome current bottlenecks in epilactose production. This approach may enable the sustainable valorization of lactose-rich byproducts and support the development of non-thermal, clean-label technologies for producing functional ingredients. Full article

Background/Objectives: The global increase in multidrug-resistant (MDR) bacterial infections underscores the urgent need for effective and sustainable antimicrobial alternatives. This study investigates the antimicrobial activity of exometabolite-based formulations (ExAFs), derived from the cell-free supernatants (CFS) of native lactic acid bacteria (LAB) applied individually or in combination thereof, against MDR-Escherichia coli strain L1PEag1. Methods: Fourteen ExAFs were screened for inhibitory activity using time–kill assays, and structural damage to bacterial cells was assessed via scanning and transmission electron microscopy (SEM/TEM). The most potent formulation was further characterized by liquid chromatography–tandem mass spectrometry (LC–MS/MS) employing a Sequential Windowed Acquisition of All Theoretical Fragment Ion Mass Spectra (SWATH) approach for untargeted metabolite profiling. Results: Among the tested formulations, E10, comprising CFS from Weissella cibaria UTNGt21O, exhibited the strongest inhibitory activity (zone of inhibition: 17.12 ± 0.22 mm), followed by E1 (CFS from Lactiplantibacillus plantarum Gt28L and Lactiplantibacillus plantarum Gt2, 3:1 v/v) and E2 (Gt28L CFS + EPS from Gt2, 3:1 v/v). Time–kill assays demonstrated rapid, dose-dependent bactericidal activity: E1 and E10 achieved >98% reduction in viable counts within 2–3 h, at 1× MIC, while E2 sustained 98.24% inhibition over 18 h, at 0.25× MIC. SEM and TEM revealed pronounced ultrastructural damage, including membrane disruption, cytoplasmic condensation, and intracellular disintegration, consistent with a membrane-targeting mode of action. Metabolomic profiling of E10 identified 22 bioactive metabolites, including lincomycin, the proline-rich peptide Val–Leu–Pro–Val–Pro–Gln, multiple flavonoids, and loperamide. Several compounds shared structural similarity with ribosomally synthesized and post-translationally modified peptides (RiPPs), including lanthipeptides and lassopeptides, suggesting a multifaceted antimicrobial mechanism. Conclusions: These findings position ExAFs, particularly E10, as promising, peptide-rich, bio-based antimicrobial candidates for food safety or therapeutic applications. The co-occurrence of RiPP analogs and secondary metabolites in the formulation suggests the potential for complementary or multi-modal bactericidal effects, positioning these compounds as promising eco-friendly alternatives for combating MDR pathogens. Full article