Search Results (13)

The grey squirrel, an invasive species, threatens the Eurasian red squirrel’s conservation, particularly in Umbria, Italy. Understanding its reproductive biology is essential to limiting its reproductive success. This study investigates the NGF system and its receptors (NTRK1 and p75NTR) in the testes of male grey squirrels, following prior research on female reproductive biology. NGF plays a role in testicular morphogenesis and spermiogenesis in animals and humans. As part of the LIFE Project U-SAVEREDS, eighteen squirrels were captured and classified into three morphotypes (immature, pubertal, and active spermatogenesis). NGF and its receptors were analyzed using real-time PCR, western blotting, immunohistochemistry, and plasma levels measured via ELISA. NGF qPCR expression levels were significantly higher during puberty compared to the immature and spermatogenesis stages (p < 0.01). Immunohistochemistry revealed NGF in Leydig cells, with stronger staining in pubertal and mature squirrels, while NTRK1 was found in Leydig cells in immature squirrels and germ cells in pubertal and mature ones. NGF receptors were observed in Sertoli cells in pubertal and mature squirrels. Plasma NGF levels showed a significant upregulation in pubertal squirrels (135.80 ± 12 pg/mL) compared to those in the immature (25.60 ± 9.32 pg/mL) and spermatogenesis stages (34.20 ± 6.06 pg/mL), with a p value < 0.01. The co-localization of NGF and its receptors suggests that NGF, produced by Leydig cells, regulates testis development and reproductive success through autocrine or paracrine mechanisms, potentially involving an unidentified pathway. Full article

This study compares bio-inspired optimization algorithms for enhancing an ANN-based Maximum Power Point Tracking (MPPT) forecast system under partial shading conditions in photovoltaic systems. Four algorithms—grey wolf optimizer (GWO), particle swarm optimization (PSO), squirrel search algorithm (SSA), and cuckoo search (CS)—were evaluated, with the dataset augmented by perturbations to simulate shading. The standard ANN performed poorly, with 64 neurons in Layer 1 and 32 in Layer 2 (MSE of 159.9437, MAE of 8.0781). Among the optimized approaches, GWO, with 66 neurons in Layer 1 and 100 in Layer 2, achieved the best prediction accuracy (MSE of 11.9487, MAE of 2.4552) and was computationally efficient (execution time of 1198.99 s). PSO, using 98 neurons in Layer 1 and 100 in Layer 2, minimized MAE (2.1679) but had a slightly longer execution time (1417.80 s). SSA, with the same neuron count as GWO, also performed well (MSE 12.1500, MAE 2.7003) and was the fastest (987.45 s). CS, with 84 neurons in Layer 1 and 74 in Layer 2, was less reliable (MSE 33.7767, MAE 3.8547) and slower (1904.01 s). GWO proved to be the best overall, balancing accuracy and speed. Future real-world applications of this methodology include improving energy efficiency in solar farms under variable weather conditions and optimizing the performance of residential solar panels to reduce energy costs. Further optimization developments could address more complex and larger-scale datasets in real-time, such as integrating renewable energy sources into smart grid systems for better energy distribution. Full article

A three-phase multilevel inverter (MLI), synthesized with 31 levels in regard to its output voltage, is used to provide the AC supply to a three-phase, squirrel cage induction motor. The gating angles required for the 30 power switches on the MLI are optimized using both the genetic algorithm (GA) and the grey wolf optimizer (GWO), in which the optimal angles are determined through solving the trigonometric equations taken from Fourier analysis to target the minimum total harmonic distortion (THD) at the MLI output. A simulation model and an experimental prototype are developed for performance analysis and validation. The results demonstrate that the MLI is effectively able to produce 31 levels of three-phase AC output voltage, with the THD not exceeding 5% when loaded with a resistive load and a three-phase induction motor. The voltage and current are measured and recorded for different loads and operating conditions, including the amount of energy consumed by the load. The results of the frequency analysis demonstrate that most of the triple harmonics, which can harm the efficiency of the inverter, are cancelled. Full article

Accurate parameter estimation is crucial and challenging for the design and modeling of PV cells/modules. However, the high degree of non-linearity of the typical I–V characteristic further complicates this task. Consequently, significant research interest has been generated in recent years. Currently, this trend has been marked by a noteworthy acceleration, mainly due to the rise of swarm intelligence and the rapid progress of computer technology. This paper proposes a developed Mountain Gazelle Optimizer (MGO) to generate the best values of the unknown parameters of PV generation units. The MGO mimics the social life and hierarchy of mountain gazelles in the wild. The MGO was compared with well-recognized recent algorithms, which were the Grey Wolf Optimizer (GWO), the Squirrel Search Algorithm (SSA), the Differential Evolution (DE) algorithm, the Bat–Artificial Bee Colony Optimizer (BABCO), the Bat Algorithm (BA), Multiswarm Spiral Leader Particle Swarm Optimization (M-SLPSO), the Guaranteed Convergence Particle Swarm Optimization algorithm (GCPSO), Triple-Phase Teaching–Learning-Based Optimization (TPTLBO), the Criss-Cross-based Nelder–Mead simplex Gradient-Based Optimizer (CCNMGBO), the quasi-Opposition-Based Learning Whale Optimization Algorithm (OBLWOA), and the Fractional Chaotic Ensemble Particle Swarm Optimizer (FC-EPSO). The experimental findings and statistical studies proved that the MGO outperformed the competing techniques in identifying the parameters of the Single-Diode Model (SDM) and the Double-Diode Model (DDM) PV models of Photowatt-PWP201 (polycrystalline) and STM6-40/36 (monocrystalline). The RMSEs of the MGO on the SDM and the DDM of Photowatt-PWP201 and STM6-40/36 were 2.042717 $\times {10}^{-3}$, 1.387641 $\times {10}^{-3}$, 1.719946 $\times {10}^{-3}$, and 1.686104 $\times {10}^{-3}$, respectively. Overall, the identified results highlighted that the MGO-based approach featured a fast processing time and steady convergence while retaining a high level of accuracy in the achieved solution. Full article

Interactions between the introduced gray squirrel (Sciurus carolinensis) and the native red squirrel (S. vulgaris) play an important role in the ecological equilibrium of European forest ecosystems. However, the range dynamics of the grey squirrel and red squirrel under future climate change scenarios remain unknown. The present study examined the range dynamics of grey squirrels and red squirrels in Europe and their range overlap now and in the future based on climate change. Under the most optimistic climate change scenario (SSP126), expansion of the grey squirrel’s range was mainly predicted in Germany, France, Croatia, Serbia, and Bulgaria. Under the most pessimistic climate change scenario (SSP585), expansion of the grey squirrel’s range was predicted in vast and scattered regions. Additionally, France, Italy, and Germany were overlapping ranges for the grey squirrel and red squirrel in the future under the SSP126 scenario but not under the current conditions, suggesting that there will be new regions where grey squirrels may threaten red squirrels in the future under SSP126. The range overlaps under the SSP585 scenario but not under the current conditions were vast and scattered, suggesting that there will be new regions in the future where grey squirrel may displace red squirrels under SSP585. Despite considerable variation, we detected expansions in the grey squirrel and red squirrel ranges and an increase in overlapping ranges between grey squirrels and red squirrels in the future. Therefore, our prediction suggests increasing threats of grey squirrels toward red squirrels in Europe in the future under climate change, which may impact the ecological equilibrium of European forest ecosystems. Full article

Database Management Systems (DBMSs) are the core of management information systems. Thus, detecting security bugs or vulnerabilities of DBMSs is an essential task. In recent years, grey-box fuzzing has been adopted to detect DBMS bugs for its high effectiveness. However, the seed scheduling strategy of existing fuzzing techniques does not consider the seeds’ correctness, which is inefficient in finding vulnerabilities in DBMSs. Moreover, current tools cannot correctly generate SQL statements with nested structures, which limits their effectiveness. This paper proposes a fuzzing solution named Squill to address these challenges. First, we propose correctness-guided mutation to utilize the correctness of seeds as feedback to guide fuzzing. Second, Squill embeds semantics-aware instantiation to correctly fill semantics to SQL statements with nested structures by collecting the context information of AST nodes. We implemented Squill based on Squirrel and evaluated it on three popular DBMSs: MySQL, MariaDB, and OceanBase. In our experiment, Squill explored 29% more paths and found 3.4× more bugs than the existing tool. In total, Squill detected 30 bugs in MySQL, 27 in MariaDB, and 6 in OceanBase. Overall, 19 of the bugs are fixed with 9 CVEs assigned. The results show that Squill outperforms the previous fuzzer in terms of both code coverage and bug discovery. Full article

The introduction of the Eastern grey squirrel (Sciurus carolinensis) in Europe is one of the best-known cases of invasive alien species (IAS) colonisation, that poses a severe risk to the conservation of biodiversity. In 2003, it was released in a private wildlife park near the city of Perugia (Italy), where it is replacing the native Eurasian red squirrel (Sciurus vulgaris). The LIFE13 BIO/IT/000204 Project (U-SAVEREDS) was set up for the Sciurus vulgaris conservation in Umbria through an eradication campaign of grey squirrels. One hundred and fifty-four animals were analysed for bacteriological, mycological, virological, and serological investigations (C4 action). Sanitary screening showed that Sciurus carolinensis is a dermatophyte carrier, and therefore, it could cause public health issues for humans, considering its confident behaviour. Moreover, it has been marginally responsible for the spreading of Candida albicans, Coxiella burnetii, and Borrelia lusitaniae. Health status evaluation conducted on the Sciurus carolinensis population indicated that it is necessary to raise awareness of its impacts on biodiversity and human health. Moreover, the health status and behaviours of the IAS must be considered when control or eradication campaigns are planned. Full article

Native red squirrels (Sciurus vulgaris) persisted in the coastal mainland woodlands of northern Gwynedd whilst sympatric with an invasive grey squirrel (Sciurus carolinensis) population suppressed by culling. Squirrelpox disease in the red squirrel population was recorded in 2017 and 2020/21. An autumn 2020 outbreak was associated with only 17.4% of animals caught and marked in the preceding June known to be present in March 2021. Despite an opportunistic data collection lacking the rigour of empirical experimental design, we observed low local survival rates similar to previously published accounts reported during major squirrelpox outbreaks. The use of a conservation dog to detect red squirrel carcasses resulted in positive detection and confirmation of a temporal and spatial expansion of one disease outbreak. The study is the first in Wales to use conservation dogs and the findings reinforce the vital strategic importance of geographical isolation reducing sympatry of red with grey squirrels in European regions where the introduced congener is a source of the squirrelpox infection. Full article

Red squirrels (Sciurus vulgaris) are native to most of Eurasia; in much of the United Kingdom, they have been supplanted by the non-native grey squirrel, and are considered an endangered species. Very little is known about the range of tick-borne pathogens to which UK red squirrels are exposed. As part of trap-and-release surveys examining prevalence of Mycobacterium spp. in red squirrel populations on two UK islands, Ixodes ricinus ticks were removed from squirrels and PCR screened for Borrelia spp., intracellular arthropod-borne bacteria and the parasitic wasp Ixodiphagus hookeri. At both sites, the most commonly encountered tick-transmitted bacterium was Borrelia burgdorferi sensu lato (overall minimum prevalence 12.7%), followed by Anaplasma phagocytophilum (overall minimum prevalence 1.6%). Single ticks infected with Spiroplasma were found at both sites, and single ticks infected with Borrelia miyamotoi or an Ehrlichia sp. at one site. Ticks harbouring Wolbachia (overall minimum prevalence 15.2%) were all positive for I. hookeri. Our study shows that UK red squirrels are potentially exposed to a variety of bacterial pathogens via feeding ticks. The effects on the health and survival of this already vulnerable wildlife species are unknown, and further studies are needed to evaluate the threat posed to red squirrels by Borrelia and other tick-borne pathogens. Full article

The grey squirrel is an invasive alien species that seriously threatens the conservation of the native red squirrel species. With the aim of characterizing the reproductive physiology of this species due to its great reproductive success, the function of the ovarian nerve growth factor (NGF) system was analyzed in a grey squirrel population living in central Italy. During the breeding and nonbreeding seasons, the ovarian presence, distribution, and gene expression of NGF, neurotrophic tyrosine kinase receptor 1 (NTRK1), and nerve growth factor receptor (NGFR), as well as NGF plasma concentrations, were evaluated in female grey squirrels. NGF was found in the luteal cells and in the thecal and granulosa cells of follicles, while NTRK1 and NGFR were only observed in follicular thecal and granulosa cells. NGF and NGFR transcripts were almost two-fold greater during the breeding season, while no seasonal differences were observed in NTRK1 gene expression. During the breeding season, NGFR was more expressed than NTRK1. Moreover, no changes were observed in NGF plasma levels during the reproductive cycle. The NGF system seems to be involved in regulating the ovarian cycle mainly via local modulation of NGF/NGFR, thus playing a role in the reproductive physiology of this grey squirrel population. Full article

This paper presents an altered grey wolf optimization, the Taguchi method, and finite element analysis (FEA) with two-phase multi-objective optimization for the design of a six-phase copper squirrel cage rotor induction motor (SCSCRIM). The multi-objective optimization design with high-performance property aims to achieve lower starting current, lower losses, lower input power, higher efficiency, higher output torque, and higher power factor. The multi-objective optimization design with high-performance property using the altered grey wolf optimization, the Taguchi method, and FEA in the first-phase program is used for minimizing the starting current, stator iron loss, stator copper loss, and input power. The multi-objective optimization design with high-performance property using the altered grey wolf optimization, the Taguchi method, and FEA in the second-phase program is used for maximizing the efficiency, output torque, and power factor. Finally, the proposed skill with higher performances is evaluated and verified via a two-phase program design and some performance tests. Full article

The reproductive cycle of an invasive alien Italian grey squirrel population was studied to understand its adaptation and limit its spread, in order to conserve the autochthonous red squirrel. Female and male genital traits were evaluated throughout the reproductive cycle, including the ovary, uterus, testicle, epididymis, seminiferous tubule morphometry, and germinative epithelium histology. Moreover, individual female fecundity was determined by counting uterine scars. Ovary width and uterus weight, length, and width reached their highest values in the luteal and pregnancy phases. On conducting a histological evaluation of the testicular germinal epithelium, four morphotypes related to the different reproductive phases of the male squirrels were identified: immature, pubertal, spermatogenesis, and regressive. Testicle and epididymis weights and seminiferous tubule diameters reached their largest values during spermatogenesis. Uterine scar analysis showed that 69% of the females had given birth to one or two litters, while 31% had no uterine scars. Litters were larger in the first breeding period than in the second; annual fecundity was 4.52 ± 1.88 uterine scars/female. Umbrian grey squirrels have adapted to their non-native range, showing two annual mating periods at times similar to those in their native range, and high reproductive success. Full article

A model for the interactions of the invasive grey squirrel species as asymptomatic carriers of the poxvirus with the native red squirrel is presented and analyzed. Equilibria of the dynamical system are assessed, and their sensitivity in terms of the ecosystem parameters is investigated through numerical simulations. The findings are in line with both field and theoretical research. The results indicate that mainly the reproduction rate of the alien population should be drastically reduced to repel the invasion, and to achieve disease eradication, actions must be performed to reduce the intraspecific transmission rate; also, the native species mortality plays a role: if grey squirrels are controlled, increasing it may help in the red squirrel preservation, while the invaders vanish; on the contrary, decreasing it in favorable situations, the coexistence of the two species may occur. Preservation or restoration of the native red squirrel requires removal of the grey squirrels or keeping them at low values. Wildlife managers should exert a constant effort to achieve a harsh reduction of the grey squirrel growth rate and to protect the remnant red squirrel population. Full article