Search Results (3,873)

Background: As part of the research project ‘Seigungo–Gungo’s Health, Education, and Maternal and Child Quality of Life: An Action-Research Project’, a study was conducted in the Gungo community in Angola, a region facing significant challenges in terms of access to healthcare and health literacy. The primary aim of the study was to evaluate the effectiveness of an intervention training model designed to improve the health literacy of the participants. Methods: The sample consisted of 30 trainees, 60% of whom were male, with an average age of 45.6 years. Most participants were single (53.3%) and had completed 6 years of formal education (26.7%). Health literacy levels were assessed using the HLS-EU-PT-Q16, a short 16-item questionnaire designed to assess three key domains: healthcare, disease prevention, and health promotion. These domains are related to the focus of the training programme. Data collection took place throughout the year 2024. Results: According to the data obtained, prior to attending the training program, 60% of the participants demonstrated an inadequate level of health literacy. Following the intervention, this percentage dropped significantly to 20%. In contrast, the proportion of participants with sufficient to excellent health literacy rose from 16.7% to approximately 40%. The results indicate that the training program had a positive and statistically significant impact on improving health literacy in the Gungo community. Conclusions: These findings highlight the importance of targeted training and sustained intervention efforts to address the specific health education needs currently affecting this community. Full article

The effects of the COVID-19 pandemic restricted the availability of healthcare and social services. This retrospective study reports WIC enrollment rates and presents descriptive data on prenatal care access and selected maternal health conditions among pregnant women in Florida before and during the COVID-19 pandemic. Using birth data linking maternal and infant characteristics from the Florida Department of Health Bureau of Vital Statistics, we examined birth records from 1 January 2019 to 31 December 2020 related to women ranging from 11 to 59 years of age who received WIC. The descriptive results show that WIC recipients had higher rates of inadequate prenatal care and adverse maternal health outcomes during the pandemic. Logistic regression results show that the odds of receiving inadequate prenatal care increased by 24% (OR = 1.24, p < 0.001), the odds of experiencing gestational diabetes by 9% (OR = 1.09, p < 0.001), and the odds of experiencing gestational hypertension by 10% (OR = 1.10, p < 0.001). Further research is needed to evaluate how specific WIC services influence maternal outcomes, particularly during public health emergencies. Full article

Background: Deferred cord clamping (DCC) is beneficial for preterm infants, but there are concerns about the safety of DCC during Cesarean deliveries (CD) under general anesthesia (GA). We evaluated maternal and neonatal outcomes in preterm CD under GA vs. regional anesthesia (RA) after implementing 180 s of DCC. Methods: This retrospective single-center observational study included CD at <33 weeks gestation, delivered between January 2018 and December 2023. The cord was clamped before 180 s for concerns of maternal bleeding or infant apnea after 30–45 s stimulation. Data was collected from reports from electronic medical records, neonatal intensive care unit database, and manually from the medical records of the patient. Multivariable regression analysis was used to assess the effect of anesthesia type and DCC on outcomes, adjusting for confounders. Results: This study included 170 mothers and 194 infants, and 84.9% of the infants received DCC ≥ 60 s. The GA group had a higher percentage of emergency CD and a lower median duration of DCC (105 s vs. 180 s, p ≤ 0.001) compared to RA. In multivariate regression analysis, GA was associated with lower odds (95% CI) of umbilical artery pH < 7 [0.1, (0.0, 0.6)], base deficit ≥ 16 [0.0, (0.0, 0.5)], and higher odds of necrotizing enterocolitis [28.2, (1.4, 560.0)]. GA was not associated with maternal hemorrhage, delivery room (DR) resuscitation, or other major neonatal morbidities or mortality. DCC ≥ 60 s was associated with lower maternal blood loss [Regression coefficient −698, (−1193, −202)], lower odds of transfusion [0.4, (0.1, 1.0)], DR resuscitation [0.4, (0.2, 0.8)], and chronic lung disease [0.4, (0.2, 0.9)], and higher survival without major morbidities [2.8, (1.2, 6.8)]. Conclusions: DCC was performed in a majority of CD under GA by adhering to protocols to shorten DCC in cases where maternal or fetal safety was threatened. GA with DCC was not associated with increased neonatal resuscitation or major neonatal morbidities and was associated with lower maternal hemorrhage and transfusion. Full article

Maternal exposure to polycyclic aromatic hydrocarbons (PAHs) during pregnancy may have effects on the offspring epigenome. And the change in onset epigenome may be associated with children’s neurodevelopment. The current study investigated the relationship between 5-hydroxymethylcytosine (5-hmC) levels in cord blood and PAH metabolites in maternal urine at delivery and children’s neurodevelopment at birth and at age 2. We enrolled 400 pregnant women and their newborns and collected their biological samples after obtaining written informed consent. Enzyme linked immunosorbent assay kits and Chromatin immunoprecipitation kits were used to assess the DNA hydroxymethylation level in cord blood. We observed that 1-hydroxypyrene (1-OHPyr) was inversely associated with gesell developmental scale scores, positively associated with global DNA 5-hmC levels, and associated with decreased 5-hmC levels of the brain-derived neurotrophic factor (BDNF) and methyl CpG binding protein 2 (MeCP2) gene promoter. In addition, the 5-hmC levels of the BDNF and MeCP2 gene promoters were associated with motor scores. The global DNA 5-hmC was inversely associated with motor scores. Mediation analysis showed mediation effects between 1-OHPyr and motor scores by 5-hmC. The global DNA 5-hmC and MeCP2 and BDNF gene promoter 5-hmC contributed 28.51%, 27.29%, and 18.98% of the effect on motor scores changes related to 1-OHPyr. The study results suggested that 5-hmC can be a potential mechanism between prenatal PAH exposure and children’s neurodevelopment at age 2 and provide a better understanding of the role of hydroxymethylation in neurodevelopment. Full article

Background: Intrahepatic cholestasis of pregnancy (ICP) is the most common reversible liver disorder linked to pregnancy, characterised by pruritus and elevated serum bile acids (BAs). Condition severity correlates with increased maternal and neonatal complications, and recent evidence highlights a significantly elevated risk of adverse perinatal outcomes, including stillbirth, when BA > 100 µmol/L. Methods: This retrospective study, conducted at a tertiary perinatology centre between 2019 and 2023, was performed in two phases. In the first phase, baseline group characteristics and pregnancy outcomes were compared between ICP and non-ICP (control) groups. In the second phase, outcomes were analysed across three ICP severity subgroups: mild (BA < 40 µmol/L), moderate (BA 40–99 µmol/L), and severe (BA ≥ 100 µmol/L). Results: A total of 210 patients diagnosed with ICP and 24,177 controls were included in the analysis. After multivariable regression, the results indicated that patients with severe ICP (BA ≥ 100 µmol/L) experienced significantly worse perinatal outcomes compared to those with mild or moderate disease: spontaneous preterm birth occurred in 26.7% of cases (p = 0.002), iatrogenic preterm birth in 36.7% (p < 0.001), meconium-stained amniotic fluid in 43.3% (p = 0.001), and neonatal intensive care unit (NICU) admission in 23.3% (p = 0.006). This subgroup also had the lowest mean birth weight (2830 g, p < 0.001). Notably, no stillbirths were recorded in any of the subgroups. Compared to controls, no major differences in maternal characteristics were noted, except in pregnancies conceived via in vitro fertilisation (IVF, p = 0.012) and those complicated by gestational diabetes (p = 0.040), both showing elevated risk for ICP development. Conclusions: This study confirms an association between ICP and increased perinatal complications, with severity of disease correlating with poorer outcomes. The findings highlight the need for standardised BA testing and improved strategies for perinatal management. Full article

This study investigated the effects of genotype and sex on carcass traits, myosin heavy chain (MyHC) isoforms, and meat quality in pigs raised under Thai commercial conditions. Fifty pigs (25 barrows and 25 gilts) from five genotypes—purebred Duroc (D), LWLR1 (Large White_old × Landrace_old), LWLR2 (Large White_new × Landrace_new), DLWLR1 (Duroc × LWLR1), and DLWLR2 (Duroc × LWLR2)—were slaughtered at ~110 kg body weight. LWLR1 is traditional maternal line, whereas LWLR2 is a newly selected line for higher intramuscular fat (IMF). Significant genotype × sex interactions were observed for dressing percentage, carcass length, bone percentage, purge loss, pH₂₄, and L* (lightness) (p < 0.05). The Longissimus lumborum (LL) muscle of Duroc pigs showed the lowest pH₄₅ and shear force but highest IMF, whereas LWLR1 pigs exhibited higher crude protein, polyunsaturated fatty acids, and flavor-enhancing nucleotides. The LL muscle of LWLR2 and DLWLR2 pigs showed greater expression of MyHC I and IIA, as well as calpain 1 (CAPN1), calpain 2 (CAPN2), and calpastatin (CAST) genes. In contrast, Duroc pigs had the highest MyHC IIX and lowest IIB expression, opposite to patterns in LWLR1 and DLWLR1. Principal component analysis revealed distinct genotype-based clustering, emphasizing the influence of maternal lineage on pork quality and offering valuable insights for genetic selection strategies. Full article

Background/Objectives: In Angola, malnutrition contributes each year to the deaths of an estimated 42,000 to 76,000 children under the age of 5. Addressing this issue must stand as a priority and requires providing local residents with access not only to nutritious food but also to adequate and accurate information in order to facilitate informed dietary choices. As part of the “Seigungo—Health, Education and Quality of Maternal and Child Life in Gungo project”, a nutrition-focused study was conducted in Gungo, Angola to evaluate the effectiveness of a training model designed to enhance food literacy among residents. Methods: Data were collected using a 14-item questionnaire developed to assess various key domains of food literacy: information seeking and access; comprehension and thematic knowledge; critical evaluation of information and behaviour; practical application and sound decision-making. Results: Thirty trainees took part in the study, of which 60% were men, with a mean age of 45.6 years. The majority were single (53.3%) and had completed six years of formal education (26.7%). Before attending the training program, 86.7% of the participants demonstrated inadequate or problematic food literacy. Following the intervention, the proportion of participants with adequate food literacy increased significantly from 13.3% to 73.3% (p < 0.001). Conclusions: The training program had a statistically significant impact on improving food literacy. Full article

Seed size is a key trait affecting evolution and agronomic performance by influencing seedling establishment in natural populations and crop yields. The Arabidopsis thaliana Seed Size QTL1 (SSQ1) locus explains 10–15% of the variation in seed size. We report here that the causal gene for this locus is Tetratricopeptide Repeat Protein 2 (TPR2), which encodes a co-chaperone. Expressing TPR2 across ecotypes and genotypes showed consistent dosage effects. Each additional TPR2^Col-0 allele increased seed mass and volume by 10–14% with high reliability in Col-0, Sha, Tsu-1, and tsu2 genetic backgrounds. Reciprocal genetic crosses indicated that this locus acts maternally, consistent with female sporophytic or female gametophytic mutations. To elucidate how TPR2 regulates seed size, the biomass composition of seeds was measured. While oil content remained unchanged, sucrose levels were markedly elevated in TPR2^Col-0 transformant lines and reduced in tpr2 mutants. Interestingly, heterologous expression of TPR2^Col-0 across genetic backgrounds increased seed protein accumulation by 18% on average. Based on these changes in sucrose and protein levels, potential modes of action for TPR2 are discussed. Full article

As newborn screening (NBS) programs deal with growing complexities, including adding new disorders to their screening panels, adopting new technologies/screening methods, and workforce shortages, there is a greater need for continuous quality improvement (CQI) to ensure the NBS system is meeting its primary goal of identifying infants with NBS disorders in a timely fashion. In 2019, the Health Resources and Services Administration’s (HRSA) Maternal and Child Health Bureau (MCHB) awarded funding to the Association of Public Health Laboratories’ (APHL) Newborn Screening Technical assistance and Evaluation Program (NewSTEPs) to address CQI in the NBS system through a collaborative, data-driven process. From 2019–2024, NewSTEPs funded 36 quality improvement (QI) projects from a variety of state NBS programs and research centers across the U.S., to address timeliness, detection of out-of-range results, communication of results, and/or confirmation of diagnosis. Thirty-three QI teams completed their projects, and 85% achieved their specified goal outlined in their aim statement. Despite limitations, the QI Projects Collaborative provided NBS programs with funding and resources to begin and sustain quality improvement initiatives. This model of a technical assistance and central resource center for CQI was effective in achieving quality improvements within the national NBS system. Full article

Background: Primordial germ cells (PGC) are the progenitor cells of sperm and eggs during the embryonic stage. The maternal gene vasa has been widely studied for its role in PGC origin, and other genes like dead end (dnd) have also been identified. Objectives: Spotted sea bass is an important economic marine fish, and the study of its germ cell characteristics provides important basic data for future population breeding and protection. Methods: In this study, we cloned the full-length sequences of Lmvasa (2384 bp, encoding 1905 aa) and Lmdnd (1523 bp, encoding 386 aa) using RACE. Temporal and spatial expression patterns of Lmvasa and Lmdnd in embryos and gonads were analyzed by PCR, immunohistochemistry, and in situ hybridization. We also used microinjections of chimeric RNA containing GFP and Lmvasa 3′ UTR to visualize PGCs. Results: Our results showed that Lmvasa and Lmdnd are expressed primarily in early embryonic development (pre-blastula stage) and were expressed only in the gonads. Immunohistochemistry revealed abundant expression of Lmvasa and Lmdnd proteins in spermatogonia, weak expression in spermatocytes, and no expression in spermatozoa. In ovaries, both genes were expressed throughout oogenesis. Furthermore, PGCs in spotted sea bass belonged to an early localization pattern. Microinjection experiments demonstrated that Lmvasa 3′ UTR effectively labeled PGCs in embryos of spotted sea bass, zebrafish, and medaka. Conclusions: These findings may contribute to understanding PGC development in spotted sea bass and other Percidae. Full article

Exclusive breastfeeding for the first six months is the gold standard in infant nutrition, yet rates in Europe remain below recommendations. This descriptive cross-sectional study identified maternal, perinatal and social determinants associated with shorter exclusive breastfeeding duration in the Algarve region. Statistically significant factors included in-hospital formula use, caesarean section, lack of skin-to-skin contact, and negative or absent previous experience of breastfeeding. In-hospital formula use and previous breastfeeding duration emerged as key predictors. These findings emphasise the importance of integrated strategies across the healthcare, workplace, and policy sectors, aligned with the lived experiences of women, to effectively support breastfeeding. Full article

Congenital heart disease (CHD) is the most prevalent birth defect. Ozone and heatwave exposure during pregnancy could increase the risk of adverse birth outcomes. Green space might be associated with beneficial birth outcomes. The research on the combined effects of those exposures on CHD is limited. Therefore, we conducted a multicenter case–control study based on a surveillance system in Zhejiang Province, China, to explore the effect of ozone, heatwave, and green space exposure during early pregnancy on CHD and their interaction. The inverse distance weighting method and normalized difference vegetation index were applied to assess maternal ozone and green space exposure, respectively. The heatwave definition is from the National Oceanic and Atmospheric Administration. Our study reveals positive associations of heatwave and ozone exposure with CHD (ozone: OR = 1.07, 95% CI: 1.02, 1.13; heatwave: OR = 1.29, 95% CI: 1.18, 1.40), and green space in different buffers around residence exerted protective effects on CHD, with ORs ranging from 0.93 to 0.94. Associations between ozone and CHD were weakened among participants with higher NDVI. Ozone’s effects on CHD were stronger with the increased duration of heatwave exposure. Our study indicates that ozone and heatwave exposure could increase the risk of CHD, and high green space is a protective factor for CHD. Meanwhile, high green space exposure could attenuate the effect of ozone on CHD, but heatwave exposure strengthened it. Full article

Introduction: Adequate maternal vitamin D status during pregnancy is essential for fetal skeletal development and neonatal vitamin D reserves. Evidence from Central and Eastern Europe on maternal deficiency, its determinants, and supplementation effectiveness in late pregnancy is limited. This study assessed the impact of 2000 IU/day and 4000 IU/day maternal vitamin D supplementation during the third trimester, compared to no supplementation, on maternal and neonatal 25-hydroxyvitamin D [25(OH)₂D] levels at birth, and explored sociodemographic, obstetric, dietary, and lifestyle factors affecting vitamin D status. Methods: In a cross-sectional study at Târgu Mureș County Clinical Hospital, Romania, 322 term mother–newborn pairs (37–41 weeks) from January 2021 to July 2023 were evaluated. The maternal and umbilical cord 25(OH)₂D was measured via electrochemiluminescence immunoassay. Data on socioeconomic status, parity, sun exposure, diet, and supplementation were collected through questionnaires and records. Statistical analysis included chi-square, linear regression, and multivariate modeling. Results: Vitamin D insufficiency and deficiency affected 32.3% and 18.9% of mothers, respectively. Supplementation was the strongest predictor of sufficiency (p < 0.01), showing a dose–response effect (r = 0.84, p < 0.01). Maternal and neonatal 25(OH)₂D levels were strongly correlated (r = 0.99, p < 0.01). Although several factors correlated with deficiency in univariate analyses, only supplementation remained significant in multivariate models. No link was found between 25(OH)₂D status and neonatal anthropometrics or early complications. Conclusions: A high prevalence of vitamin D deficiency has been documented among pregnant women in Romania. High-dose supplementation during late pregnancy is critical to ensure sufficient maternal and neonatal vitamin D, highlighting the need for standardized antenatal supplementation protocols, especially in disadvantaged groups. Full article

During pregnancy, exposure to fine particulate matter (PM_2.5), particularly diesel exhaust particles (DEPs), elevates the risk of placental dysfunction-related pregnancy complications; however, the underlying cellular mechanisms have yet to be fully elucidated. The objective of this study was to assess the effects of PM_2.5 exposure on trophoblast functions and their interaction with endometrial stromal cells. We utilized a three-dimensional (3D) model in which human first-trimester trophoblasts (Sw71) formed blastocyst-like spheroids and were cultured with human endometrial stromal cells (HESCs). Trophoblast proliferation, migration, invasion, and 3D network formation following DEP exposure (0.5–20 μg/mL) were assessed using methyl thiazolyl diphenyl-tetrazolium bromide (MTT), wound healing, migration, and invasion assays. The expression levels of genes related to the epithelial-mesenchymal transition (EMT) were quantified by real-time reverse-transcription quantitative polymerase chain reaction (RT-qPCR). DEP exposure significantly inhibited trophoblast proliferation, migration, and invasion. DEP treatment dysregulated the EMT program by significantly decreasing the expression of key mesenchymal markers (SNAI1, SNAI2, SOX2, and KLF4) while upregulating epithelial markers. These changes may be related to inhibited trophoblast migration toward HESC monolayers and 3D invasive network formation. DEP directly impairs critical trophoblast functions that are essential for successful pregnancy. Disruption of the EMT program represents a molecular mechanism by which traffic-related air pollution contributes to placental dysfunction and pregnancy complications, highlighting the significant reproductive risks posed by ambient air pollution. Full article

Discrimination has been linked to greater stress and higher levels of depressive symptoms. However, there has been no research to date that has examined how life course inequality due to everyday discrimination is associated with mental health outcomes later in life. Using data from Waves I, IV, and V of the National Longitudinal Study of Adolescent to Adult Health (Add Health) from 1994 to 2018, we examined how everyday discrimination in young adulthood (Wave IV) was associated with depressive symptoms at early midlife (Wave V). We also examined how parent–child relationships in young adulthood (maternal and paternal closeness; satisfaction of communication with mothers and fathers) moderated this association. We analyzed two sub samples: a mother sample (n = 9390) and a father sample (n = 8229). Results from both showed that everyday discrimination in young adulthood was significantly associated with depressive symptoms at early midlife, and parent–child relationships served as significant protective factors against depression. Mental health policy and intervention efforts should address how discrimination experienced in young adulthood can have enduring adverse effects on mental health into early midlife and invest in strategies that promote supportive parent–child relationships as protective resources. Full article