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17 pages, 7612 KB  
Article
Canopy-Mediated Shifts in Grassland Diversity and Heterogeneity: A Power Law Approach from China’s Loess Plateau
by Lili Qian, Cong Wu, Sipu Jing, Li Meng, Shuo Liu, Xiangyang Hou, Wenjie Lu and Xiang Zhao
Plants 2025, 14(19), 3008; https://doi.org/10.3390/plants14193008 (registering DOI) - 28 Sep 2025
Abstract
This study investigates the spatial heterogeneity and species diversity of grassland vegetation in the agro-pastoral ecotone of China’s Loess Plateau, integrating Taylor’s power law model with the minimum area concept to address scale-dependent ecological patterns. Field surveys were conducted across four vegetation types: [...] Read more.
This study investigates the spatial heterogeneity and species diversity of grassland vegetation in the agro-pastoral ecotone of China’s Loess Plateau, integrating Taylor’s power law model with the minimum area concept to address scale-dependent ecological patterns. Field surveys were conducted across four vegetation types: small-leaf poplar forest (SP), pine–caragana mixed forest (PC), caragana shrubland (RC), and saline grassland (SG). Nested quadrats (0.25–8 m2) were used to establish species–area relationships (SARs), while binary occurrence frequency data fitted to Taylor’s power law quantified spatial heterogeneity parameters (δi, δc, CACD) and derived diversity indices (H′, J′, D). the results showed that species composition differed significantly among vegetation types, with RC exhibiting the highest richness (25 species) and SG the lowest (12 species). SAR analysis showed distinct z-values: SP had the lowest z (0.14), indicating minimal area effects and high homogeneity, while SG had the highest area sensitivity. Spatial heterogeneity (δc) was highest in RC and lowest in SP. Over 82.5% of herb-layer species exhibited aggregated distributions (δi > 0). The dominant species Leymus secalinus (Georgi) Tzvelev shifted from regular (δi < 0) under SP/SG to aggregated (δi > 0) under PC/RC. Diversity metrics peaked in PC plots (highest H′ and richness, lowest dominance), whereas SP showed high dominance but low diversity. CACD values (critical aggregation diversity) were maximized under SG. The integration of power law modeling and minimum area analysis effectively captures scale-dependent vegetation patterns. Pine–caragana mixed forests (PC) optimize biodiversity and spatial heterogeneity, suggesting moderated canopy structures enhance ecological stability. These findings provide a theoretical basis for sustainable grassland management in ecologically sensitive agro-pastoral zones. Full article
(This article belongs to the Section Plant Modeling)
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25 pages, 13748 KB  
Article
Differential Corrosion Behavior of High-Aluminum 304 Stainless Steel in Molten Nitrate Salts: The Roles of Rolling and Heat Treatment
by Weijie Tang, Kan Zhou, Zhenguo Li, Lifu Xin, Dexian Huang, Faqi Zhan, Penghui Yang, Haicun Yu and Peiqing La
Materials 2025, 18(19), 4513; https://doi.org/10.3390/ma18194513 (registering DOI) - 28 Sep 2025
Abstract
The high material cost has restricted the development of concentrated solar power (CSP) systems. In this study, a low-cost alternative material was developed by adding aluminum to 304 stainless steel to form a protective oxide film, thereby enhancing its corrosion resistance to molten [...] Read more.
The high material cost has restricted the development of concentrated solar power (CSP) systems. In this study, a low-cost alternative material was developed by adding aluminum to 304 stainless steel to form a protective oxide film, thereby enhancing its corrosion resistance to molten salt. Three material variants were tested: untreated hot-rolled plates after solution treatment and cold-rolled high-aluminum 304 stainless steel (High-Al304SS) after solution treatment and annealing treatment. After all samples were immersed in a NaNO3-KNO3 mixed salt at 600 °C for 480 h, corrosion products including NaFeO2, CrO2, Mn2O4, and NiCr2O4 were formed. The phase composition was determined by XRD, and the surface and cross-section of the corrosion layer were analyzed by SEM and EDS surface and point analysis. The corrosion rate of the samples was calculated by the weight loss method. Notably, an Al2O3-Cr2O3 composite oxide film was formed on the sample surface, effectively inhibiting corrosion. The high defect density and grain boundary energy introduced by the cold-rolling process, as well as the precipitation of the second phase during annealing, accelerated the corrosion process of the samples. However, the hot-rolled samples after solution treatment exhibited excellent corrosion resistance (64.43 μm/year) and, through further process optimization, are expected to become an ideal low-cost alternative material for 347H stainless steel (23 μm/year) in CSP systems. Full article
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23 pages, 1306 KB  
Article
Mixed-Graph Neural Network for Traffic Flow Prediction by Capturing Dynamic Spatiotemporal Correlations
by Xing Su, Pengcheng Li, Zhi Cai, Limin Guo and Boya Zhang
ISPRS Int. J. Geo-Inf. 2025, 14(10), 379; https://doi.org/10.3390/ijgi14100379 (registering DOI) - 27 Sep 2025
Abstract
Traffic flow prediction is a prominent research area in intelligent transportation systems, significantly contributing to urban traffic management and control. Existing methods or models for traffic flow prediction predominantly rely on a fixed-graph structure to capture spatial correlations within a road network. However, [...] Read more.
Traffic flow prediction is a prominent research area in intelligent transportation systems, significantly contributing to urban traffic management and control. Existing methods or models for traffic flow prediction predominantly rely on a fixed-graph structure to capture spatial correlations within a road network. However, the fixed-graph structure can restrict the representation of spatial information due to varying conditions such as time and road changes. Drawing inspiration from the attention mechanism, a new prediction model based on the mixed-graph neural network is proposed to dynamically capture the spatial traffic flow correlations. This model uses graph convolution and attention networks to adapt to complex and changeable traffic and other conditions by learning the static and dynamic spatial traffic flow characteristics, respectively. Then, their outputs are fused by the gating mechanism to learn the spatial traffic flow correlations. The Transformer encoder layer is subsequently employed to model the learned spatial characteristics and capture the temporal traffic flow correlations. Evaluated on five real traffic flow datasets, the proposed model outperforms the state-of-the-art models in prediction accuracy. Furthermore, ablation experiments demonstrate the strong performance of the proposed model in long-term traffic flow prediction. Full article
(This article belongs to the Topic Artificial Intelligence Models, Tools and Applications)
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17 pages, 3548 KB  
Article
Electronic and Nuclear Subsystem Response in Hybrid Halide Perovskites Under γ-Irradiation
by Ivan E. Novoselov and Ivan S. Zhidkov
Nanomaterials 2025, 15(19), 1474; https://doi.org/10.3390/nano15191474 - 25 Sep 2025
Abstract
Lead halide perovskites, including single-cation (MAPbI3, FAPbI3, CsPbI3) and mixed-cation (Cs0.12FA0.88PbI3, Cs0.1MA0.15FA0.75PbI3) compositions, are promising for both space photovoltaics and γ-ray detection due [...] Read more.
Lead halide perovskites, including single-cation (MAPbI3, FAPbI3, CsPbI3) and mixed-cation (Cs0.12FA0.88PbI3, Cs0.1MA0.15FA0.75PbI3) compositions, are promising for both space photovoltaics and γ-ray detection due to their tunable optoelectronic properties. However, their response to high-energy radiation remains critical for reliable operation. We performed Monte-Carlo simulations using GEANT4 to investigate photon interactions (0.1–90 MeV) with perovskites of varying composition and thickness (1 cm to 1 μm). Results indicate that heavy atoms (Pb, I) dominate photoelectric absorption and scattering, broadly similar absorbed energies and event rates across compositions. Cs-containing perovskites exhibit slightly higher absorption and ionization, whereas FA- and MA-rich compositions show reduced photoelectric and Rayleigh scattering. Layer thickness strongly influences the radiation response: ultrathin films display fewer interactions with higher per-event energy, while millimeter-scale layers achieve efficient absorption and enable pair-production events at MeV energies. The sequence of dominant processes follows the expected energy dependence: photoelectric effect at low energies, Compton and Rayleigh scattering at intermediate energies, and pair production at high energies. These findings demonstrate that perovskite γ-interaction is primarily governed by heavy-atom content, with A-site cations fine-tuning the process balance, and that device performance for detection or photovoltaics depends critically on layer thickness. Full article
(This article belongs to the Section Nanoelectronics, Nanosensors and Devices)
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15 pages, 5156 KB  
Article
The Aspergillus fumigatus Extracellular Polysaccharide Galactosaminogalactan Displays Context-Dependent Cooperative and Competitive Social Traits in Mixed Biofilms
by Longyun Cong, Yufei Zhang, Hua Chen, Ruiyang Lu and Shizhu Zhang
J. Fungi 2025, 11(10), 695; https://doi.org/10.3390/jof11100695 - 25 Sep 2025
Abstract
Biofilm-dwelling cells construct communities by secreting extracellular polysaccharide (EPS). In bacteria, EPS can act as cooperative public goods or competitive traits, yet the social nature of EPS in fungi remains poorly understood. Galactosaminogalactan (GAG) is an EPS produced by the human-pathogenic fungus Aspergillus [...] Read more.
Biofilm-dwelling cells construct communities by secreting extracellular polysaccharide (EPS). In bacteria, EPS can act as cooperative public goods or competitive traits, yet the social nature of EPS in fungi remains poorly understood. Galactosaminogalactan (GAG) is an EPS produced by the human-pathogenic fungus Aspergillus fumigatus. The study of social characteristics of GAG revealed that under basal conditions, GAG can be shared between GAG production strain (GAG+) and non-production strain (GAG) in mixed biofilms. This led to significant competitive advantages for GAG, with fitness outcomes dependent on initial inoculum ratios, cultivation duration, and nutrient availability. Conversely, under cell wall stress induced by antifungal drug caspofungin, GAG confers a competitive advantage for GAG+ in the mixed biofilms. Further investigation revealed that GAG+ cells are able to retain higher levels of GAG on the hyphal surface compared to GAG in the mixed biofilms. This hyphal surface-associated GAG layer might protect GAG+ from caspofungin-mediated damage, creating a lineage-specific competitive advantage. Overall, GAG has a dual-trait social nature in biofilms, functioning as a public good at the population level and as a competitive trait for the producing lineage, switching according to environmental conditions. Full article
(This article belongs to the Section Fungal Cell Biology, Metabolism and Physiology)
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28 pages, 3039 KB  
Article
Building Anatomy: Rethinking Internal and External Dynamics in Architecture
by Murat Sönmez and Neslihan Efsa Özçelik
Buildings 2025, 15(19), 3461; https://doi.org/10.3390/buildings15193461 - 25 Sep 2025
Viewed by 59
Abstract
Conventional frameworks often reduce architectural production to a linear sequence of deterministic technical and managerial stages. This study challenges that paradigm, arguing that such a view overlooks the adaptive, multi-layered, and context-responsive nature of contemporary built environment creation. Grounded in systems theory, biomimicry, [...] Read more.
Conventional frameworks often reduce architectural production to a linear sequence of deterministic technical and managerial stages. This study challenges that paradigm, arguing that such a view overlooks the adaptive, multi-layered, and context-responsive nature of contemporary built environment creation. Grounded in systems theory, biomimicry, and human physiology, a novel “Building Anatomy” model is proposed that treats architectural practice as a living organism. This conceptual framework is first established, and its validity is then tested through a mixed-methods empirical study conducted with 126 Turkish architects, analyzing the continuous feedback loops between internal (e.g., designer identity, team dynamics) and external (e.g., regulations, socio-cultural currents) factors. It was confirmed that the dynamic interaction between these internal and external factors is central to architectural processes. “Systemic dysfunctions” were identified and diagnosed that arise from breakdowns in these metabolic feedback loops, providing empirical evidence for the model’s explanatory power. By offering a systemic lens, this study shifts the focus from a product-centric to a process-oriented view of design. The Building Anatomy model demonstrates its potential for diagnosing “metabolic failures” and redefining the architect’s agency, ultimately advocating for more adaptive, responsive, and resilient architectural outcomes. Full article
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24 pages, 4890 KB  
Article
Turbulent Hybrid Nanofluid Flow in Corrugated Channels with Vortex Generators: A Numerical Study
by Aimen Tanougast, Issa Omle and Krisztián Hriczó
Fluids 2025, 10(10), 249; https://doi.org/10.3390/fluids10100249 - 24 Sep 2025
Viewed by 52
Abstract
Nanofluids are an important technology for enhancing heat transfer in industrial applications by incorporating high thermal conductivity nanoparticles into base fluids. However, they often require higher pumping power and energy consumption. This study employs a two-dimensional (2D) approximation of vortex generators (VGs) in [...] Read more.
Nanofluids are an important technology for enhancing heat transfer in industrial applications by incorporating high thermal conductivity nanoparticles into base fluids. However, they often require higher pumping power and energy consumption. This study employs a two-dimensional (2D) approximation of vortex generators (VGs) in a turbulent trapezoidal channel with nanoparticle concentrations of Al2O3, SiO2, and TiO2. Simulations are performed using ANSYS Fluent 2021 with the Finite Volume Method (FVM) and the k–ε turbulence model to capture turbulence characteristics, eddy viscosity, and turbulent kinetic energy production. The introduction of vortex generators improves fluid mixing and reduces the thermal boundary layer, resulting in enhanced heat transfer, with a performance evaluation criterion (PEC) of 1.08 for water (baseline case without nanofluids). The single nanofluids further optimize heat transfer, increasing the Nusselt number and pressure drop while balancing thermal performance, reaching a PEC of 1.6 for SiO2 at 3% concentration, representing a 48% improvement over the baseline. A hybrid mixture of 1% Al2O3 and 2% SiO2 achieves the same PEC of 1.6 as single SiO2 nanoparticles, but with higher heat transfer and lower pressure drop, demonstrating improved thermal performance. Full article
(This article belongs to the Section Mathematical and Computational Fluid Mechanics)
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35 pages, 13854 KB  
Article
Middle Paleolithic Neanderthal Open-Air Camp and Hyena Den Westeregeln (D)—Competition for Prey in a Mammoth Steppe Environment of Northern Germany (Central Europe)
by Cajus G. Diedrich
Quaternary 2025, 8(4), 52; https://doi.org/10.3390/quat8040052 - 24 Sep 2025
Viewed by 39
Abstract
A gypsum karst sinkhole at Westeregeln (north-central Germany) was filled during the Late Pleistocene, first by fluvial flooding, then by solifluctation, and finally with wind-transported loess. Pleistocene mollusks and bones of snakes, birds, micro- and macromammals, and hyena coprolites were accumulated, often mixed [...] Read more.
A gypsum karst sinkhole at Westeregeln (north-central Germany) was filled during the Late Pleistocene, first by fluvial flooding, then by solifluctation, and finally with wind-transported loess. Pleistocene mollusks and bones of snakes, birds, micro- and macromammals, and hyena coprolites were accumulated, often mixed in gravel or sand layers with Middle Paleolithic artifacts, whereas ice wedges reach deep into the sinkhole. The high amount of small flint debris prove on-site tool production by using 99% local Saalian transported brownish-to-dark Upper Cretaceous flint, which could have been collected from the Bode River gravels near-site. Only a single quartzite and one jasper flake prove other local gravel sources or importation. A large bifacial flaked knife of layer 4 dates to the early/middle Weichselian/Wuermian (MIS 5-4), similar to two triangular handaxes in the MTA tradition and an absolutely dated woolly rhinoceros bone (50,310 + 1580/−1320 BP). A cold period of Late Pleistocene glacial mammoth steppe megafauna is represented, but the material is mostly strongly fragmented and smashed by humans. Neanderthal camp use on the gypsum hill is indicated also by small charcoal pieces, burned bone fragments, and fire-dehydrated flint fragments. Crocuta crocuta spelaea (Goldfuss) hyenas are well known from Westeregeln, with an open-air commuting den site, which was marked with feces. Full article
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19 pages, 7060 KB  
Article
Non-Invasive Multi-Analytical Insights into Renaissance Wall Paintings by Bernardino Luini
by Eleonora Verni, Michela Albano, Curzio Merlo, Francesca Volpi, Chaehoon Lee, Chiara Andrea Lombardi, Valeria Comite, Paola Fermo, Andrea Bergomi, Vittoria Guglielmi, Mattia Borelli, Carlo Mariani, Sabrina Samela, Lorenzo Vinco, Marta Ghirardello, Tommaso Rovetta, Giacomo Fiocco and Marco Malagodi
Coatings 2025, 15(9), 1113; https://doi.org/10.3390/coatings15091113 - 22 Sep 2025
Viewed by 132
Abstract
The findings of non-invasive, multi-analytical research on two wall paintings located in the Santuario della Beata Vergine dei Miracoli in Saronno (Varese, Italy)—The Marriage of the Virgin and The Adoration of the Christ Child—are presented in this paper. The authorship of [...] Read more.
The findings of non-invasive, multi-analytical research on two wall paintings located in the Santuario della Beata Vergine dei Miracoli in Saronno (Varese, Italy)—The Marriage of the Virgin and The Adoration of the Christ Child—are presented in this paper. The authorship of the latter is up for controversy, while the former is unquestionably attributed to Bernardino Luini. The objective was to assess the compatibility of their color palettes through material comparison. A complementary suite of non-invasive techniques, including X-ray fluorescence (XRF), external reflection FTIR, Raman, visible reflectance spectroscopy and hyperspectral imaging, were employed to characterize pigments and surface materials without sampling. Results confirm the use of historically consistent pigments such as calcium carbonate, ochres, Naples yellow, smalt, azurite and lapis lazuli. Differences in the application of blue pigments—lapis lazuli in The Marriage of the Virgin and azurite in The Adoration of the Christ Child—may reflect workshop variation rather than separate authorship. Spectral imaging revealed pigment mixing and layering strategies, especially in skin tones and shadow modeling. This study underscores the significance of diagnostics as an interpretive instrument, capable of contextualizing Luini’s paintings within the context of Renaissance creative practice, providing a framework relevant to analogous inquiries. Full article
(This article belongs to the Special Issue Surface and Interface Analysis of Cultural Heritage, 2nd Edition)
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26 pages, 43661 KB  
Article
Numerical Investigation of Atwood Number Effects on Shock-Driven Single-Mode Stratified Heavy Fluid Layers
by Salman Saud Alsaeed, Satyvir Singh and Nouf A. Alrubea
Mathematics 2025, 13(18), 3032; https://doi.org/10.3390/math13183032 - 19 Sep 2025
Viewed by 149
Abstract
This work presents a numerical investigation of Richtmyer–Meshkov instability (RMI) in shock-driven single-mode stratified heavy fluid layers, with emphasis on the influence of the Atwood number. High-order modal discontinuous Galerkin simulations are carried out for Atwood numbers ranging from A=0.30 to [...] Read more.
This work presents a numerical investigation of Richtmyer–Meshkov instability (RMI) in shock-driven single-mode stratified heavy fluid layers, with emphasis on the influence of the Atwood number. High-order modal discontinuous Galerkin simulations are carried out for Atwood numbers ranging from A=0.30 to 0.72, allowing a systematic study of interface evolution, vorticity dynamics, and mixing. The analysis considers diagnostic quantities such as interface trajectories, normalized interface length and amplitude, vorticity extrema, circulation, enstrophy, and kinetic energy. The results demonstrate that the Atwood number plays a central role in instability development. At low A, interface deformation remains smooth and coherent, with weaker vorticity deposition and delayed nonlinear roll-up. As A increases, baroclinic torque intensifies, leading to rapid perturbation growth, stronger vortex roll-ups, and earlier onset of secondary instabilities such as Kelvin–Helmholtz vortices. Enstrophy, circulation, and interface measures show systematic amplification with increasing density contrast, while the total kinetic energy exhibits relatively weak sensitivity to A. Overall, the study highlights how the Atwood number governs the transition from linear to nonlinear dynamics, controlling both large-scale interface morphology and the formation of small-scale vortical structures. These findings provide physical insight into shock–interface interactions and contribute to predictive modeling of instability-driven mixing in multicomponent flows. Full article
(This article belongs to the Special Issue High-Order Numerical Methods and Computational Fluid Dynamics)
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26 pages, 7813 KB  
Article
Fe–Si–O Isotope Characteristics and Ore Formation Mechanisms of the Hugushan Area BIF-Type Iron Deposits in the Central North China Craton
by Ende Wang, Deqing Zhang, Jinpeng Luan, Yekai Men, Ran Wang, Jianming Xia and Suibo Zhang
Minerals 2025, 15(9), 996; https://doi.org/10.3390/min15090996 - 19 Sep 2025
Viewed by 241
Abstract
The Hugushan banded iron formation (BIF) is one of the most representative iron ore deposits in the central part of the North China Craton, and its ore formation mechanism remains highly controversial. This study presents whole-rock and Fe–Si–O isotope geochemical evidence, offering a [...] Read more.
The Hugushan banded iron formation (BIF) is one of the most representative iron ore deposits in the central part of the North China Craton, and its ore formation mechanism remains highly controversial. This study presents whole-rock and Fe–Si–O isotope geochemical evidence, offering a new perspective on the ore formation mechanism of the Hugushan BIFs. The samples from the upper and lower parts of the Hugushan BIFs are characterized by slight enrichment of heavy and light Fe isotopes, respectively. Additionally, the samples from the upper part of the Hugushan BIFs show characteristics of slightly positive Ce anomalies and negative La anomalies, suggesting that the shallow ancient seawater was in a partially oxidized state, whereas the deep seawater remained in a reductive environment during the depositional period. The low Al2O3 and TiO2 concentrations, as well as the depletion of Zr and Hf in the Hugushan BIFs, suggest that the contribution of terrestrial detrital materials to deposition is extremely limited. The BIFs all exhibit positive Eu anomalies, and the quartz in the BIFs is depleted in 30Si, a characteristic similar to that observed in siliceous rocks formed in hydrothermal vent environments and during hydrothermal plume activity. Additionally, the δ18O values of quartz in Hugushan BIFs are similar to the O isotope compositions of hydrothermal sedimentary siliceous rocks, further suggesting that the silicon in BIFs originates primarily from seafloor hydrothermal activity. The combination of Eu/Sm, Sm/Yb, and Y/Ho ratios indicates that the major components (iron and silica) of the Hugushan Iron Ore Deposit originated from the mixing of high-temperature hydrothermal fluids with seawater, with the hydrothermal fluid contributing slightly less than 0.1%. The magnetite and quartz bands in the BIFs exhibit inhomogeneous and covariant δ56Fe and δ30Si isotope characteristics, suggesting that the alternating siliceous and ferruginous layers are products of original chemical deposition in the ocean. Periodic hydrothermal activity and ocean transgression caused the recurring deposition of siliceous and ferruginous layers, resulting in the characteristic banded structure of the Hugushan Iron Ore Deposit. Full article
(This article belongs to the Special Issue Selected Papers from the 7th National Youth Geological Congress)
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13 pages, 6593 KB  
Article
Block Magnets with Uniform Core–Shell Microstructure Regenerated from NdFeB Grain Boundary Diffusion Sheet Magnets
by Xiangheng Zhuge, Shuhan Dong, Yuxin Jin, Qiong Wu, Ming Yue, Weiqiang Liu, Yuqing Li, Zhanjia Wang, Qingmei Lu, Yiming Qiu and Yanjie Tong
Nanomaterials 2025, 15(18), 1437; https://doi.org/10.3390/nano15181437 - 18 Sep 2025
Viewed by 207
Abstract
The grain boundary diffusion (GBD) process is currently the relatively effective method for utilizing heavy rare earth (HRE) elements in NdFeB magnets, especially for magnetic sheets. However, due to a highly uneven microstructure, the recovery of GBD magnets was considered difficult. In this [...] Read more.
The grain boundary diffusion (GBD) process is currently the relatively effective method for utilizing heavy rare earth (HRE) elements in NdFeB magnets, especially for magnetic sheets. However, due to a highly uneven microstructure, the recovery of GBD magnets was considered difficult. In this work, our study prioritized short-loop recycling of GBD NdFeB sheet magnets to prepare block magnets. A comparative investigation was conducted between GBD-processed NdFeB magnets and the conventional sintered magnets, with particular emphasis on their recyclability characteristics. Among them, the Tb content of GBD magnets of 0.4 wt.% was significantly lower than sintered magnets of 1.73 wt.%. When two waste magnets were supplemented with the same amount of rare earth, it was found that the coercivity of the block magnets regenerated from GBD sheet magnets was higher. Microstructural analysis revealed that the core–shell grains originally located in the surface layer of GBD magnets were uniformly mixed and diffused with the ordinary particles originally located inside during the regeneration sintering process. The regenerated GBD magnets exhibited a more uniform core–shell microstructure with submicron shells of Tb elements along with reduced areas of RE-rich phase enrichment which facilitated the formation of a continuous and uniform thin-layer grain boundary, thereby enhancing the magnetic isolation effect. Apart from the significance of recycling, these block magnets regenerated from GBD magnets also provides a new approach to solving the challenge of high coercivity and low HRE elements in bulk magnets. Full article
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14 pages, 1784 KB  
Article
Extended PRF: Impact of Heat on Gene Expression in Gingival Fibroblasts
by Xiaoyu Huang, Layla Panahipour, Dorna Rassi Faghihi, Richard J. Miron and Reinhard Gruber
Int. J. Mol. Sci. 2025, 26(18), 9120; https://doi.org/10.3390/ijms26189120 - 18 Sep 2025
Viewed by 265
Abstract
Extended platelet-rich fibrin (e-PRF) combines the prolonged resorption properties of heat-coagulated platelet-poor plasma (PPP), becoming an albumin gel (Alb-gel) that is mixed back with the respective native cell-rich buffy coat layer (BC), i.e., concentrated PRF (C-PRF). E-PRF or Alb-PRF is utilized as a [...] Read more.
Extended platelet-rich fibrin (e-PRF) combines the prolonged resorption properties of heat-coagulated platelet-poor plasma (PPP), becoming an albumin gel (Alb-gel) that is mixed back with the respective native cell-rich buffy coat layer (BC), i.e., concentrated PRF (C-PRF). E-PRF or Alb-PRF is utilized as a barrier membrane in various clinical applications, such as guided tissue regeneration. The heating of PPP might lower its biological activity, but testing this hypothesis is necessary. To this end, we exposed gingival fibroblasts to the lysates of regular PPP, heated PPP (hPPP), and BC, followed by bulk RNA sequencing. Gingival fibroblasts responded to PPP lysates with a total of 153 up- and 71 down-regulated genes when considering a minimum 3.0-fold log2 expression change and a significance level 2.0 log-10. In sharp contrast, the response to hPPP was characterized by only five up-regulated and five down-regulated genes, clearly indicating that heating almost completely abolished the biological activity of PPP. As expected, BC was more potent than PPP and broadened the spectrum of regulated genes. RT-PCR and immunoassays confirmed the heat sensitivity of PPP as exemplified by IL11 and other genes. Moreover, PPP, but not hPPP, drives the phosphorylation of p65, representing NF-κB signaling. Taken together, these findings extend previous observations that PPP causes a robust response in gingival fibroblasts and also strengthen the hypothesis that this response is heat-sensitive. These operations support the clinical concept of e-PRF by mixing back the heated inactive PPP with the bioactive buffy coat C-PRF layer. Full article
(This article belongs to the Special Issue Application of Biotechnology to Dental Treatment)
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14 pages, 4490 KB  
Article
Assessing Intra-Annual Spatial Distribution of Amphioctopus fangsiao in the East China Sea and Southern Yellow Sea Using Ensemble Models
by Yan Cui, Xiaodi Gao, Shaobo Yang, Shengfa Li and Linlin Yang
J. Mar. Sci. Eng. 2025, 13(9), 1806; https://doi.org/10.3390/jmse13091806 - 18 Sep 2025
Viewed by 269
Abstract
Understanding the distribution pattern and its drivers of species is crucial for developing effective and sustainable management strategies. Amphioctopus fangsiao is the octopus of significant commercial and ecological value along the coast of China, with multiple distinct populations. However, research on their ecological [...] Read more.
Understanding the distribution pattern and its drivers of species is crucial for developing effective and sustainable management strategies. Amphioctopus fangsiao is the octopus of significant commercial and ecological value along the coast of China, with multiple distinct populations. However, research on their ecological dynamics remains limited and requires further investigation. Here, ensemble models were constructed to examine the spatio-temporal distribution and inter-populational differentiation in environmental adaptability of A. fangsiao in the East China Sea (ECS) and the South Yellow Sea (SYS). Specifically, we generated the ensemble models by integrating Gradient Boosting Machine (GBM), Generalized Linear Models (GLMs), and Maximum Entropy Models (MaxEnt) for the different populations across four seasons, using fishery-independent data collected from 2015 to 2021. The results revealed two hotspots of A. fangsiao in the ECS and SYS: one is the area of SYS along the coastal waters, with latitudes 33° N–34° N and longitudes 120° E–122° E (northern population, NP); the other one is near the Kuroshio-adjacent area with latitudes 28.5° N–29° N and longitudes 123° E–124.5° E (southern population, SP). Both NP and SP exhibited distinct seasonal habitat preferences, with key environmental drivers showing seasonal variations. The NP tended to inhabit coastal waters with lower sea surface heights (SSHs), shallower water depth, and a narrower sea bottom salinity range (SBS). In contrast, SP preferred marine environments with a thicker mixed layer thickness (MLT) and higher concentrations of bottom chlorophyll-a (Chl_b). The environmental characterization of suitable habitats revealed distinct patterns in resource utilization and environmental adaptation strategies between the two populations. This study provides fundamental data for understanding A. fangsiao population dynamics and underscores the importance of considering population-specific habitat preferences within dynamic marine environments. Full article
(This article belongs to the Special Issue Marine Ecological Ranch, Fishery Remote Sensing, and Smart Fishery)
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21 pages, 21336 KB  
Article
A Comparative Analysis of UAV LiDAR and Mobile Laser Scanning for Tree Height and DBH Estimation in a Structurally Complex, Mixed-Species Natural Forest
by Lucian Mîzgaciu, Gheorghe Marian Tudoran, Andrei Eugen Ciocan, Petru Tudor Stăncioiu and Mihai Daniel Niță
Forests 2025, 16(9), 1481; https://doi.org/10.3390/f16091481 - 18 Sep 2025
Viewed by 305
Abstract
Accurate measurement of tree height and diameter at breast height (DBH) is essential for forest inventory, biomass estimation, and habitat assessment but remains challenging in structurally complex, multi-layered forests. This study evaluates the accuracy and operational feasibility of Unmanned Aerial Vehicle (UAV) LiDAR [...] Read more.
Accurate measurement of tree height and diameter at breast height (DBH) is essential for forest inventory, biomass estimation, and habitat assessment but remains challenging in structurally complex, multi-layered forests. This study evaluates the accuracy and operational feasibility of Unmanned Aerial Vehicle (UAV) LiDAR and Mobile Laser Scanning (MLS) for estimating tree height and DBH in such stands with a diverse structure in the Romanian Carpathians. Field measurements from six plots encompassing mixed-species (Fagus sylvatica L., Abies alba Mill., Picea abies (L.) H.Karst.) and single-species (Picea abies) stands were compared against UAV- and MLS-derived metrics. MLS delivered near-inventory-grade DBH accuracy across all species (R2 up to 0.98) and reliable height estimates for intermediate and suppressed trees, while UAV LiDAR consistently underestimated tree height, especially in dense, multi-layered stands (R2 < 0.2 in mixed plots). Voxel-based occlusion analysis revealed that over 93% of area under canopy and interior crown volume was captured only by MLS, confirming its dominance below the canopy, whereas UAV LiDAR primarily delineated the outer canopy surface. Species traits influenced DBH accuracy locally, but structural complexity and canopy layering were the main drivers of height underestimation. We recommend hybrid UAV–MLS workflows combining UAV efficiency for canopy-scale mapping with MLS precision for stem and sub-canopy structure. Future research should explore multi-season acquisitions, improved SLAM robustness, and automated data fusion to enable scalable, multi-layer forest monitoring for carbon accounting, biodiversity assessment, and sustainable forest management decision making. Full article
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