Search Results (44)

This paper proposes a bidirectional self-propelled traveling wave linear ultrasonic motor. The motor adopts a straight-beam stator structure, with two piezoelectric ceramic plates arranged at each end of the stator. One end operates in the inverse piezoelectric mode, while the other operates in the piezoelectric mode. By switching the piezoelectric/inverse piezoelectric modes at both ends, the propagation direction of the traveling wave component in the stator can be altered, thereby achieving bidirectional operation of the linear ultrasonic motor. A finite element model of the motor is established, and its performance is analyzed through modal analysis, harmonic response analysis, and transient analysis, verifying the correctness of the design. Full article

Recent advances in the understanding of facial anatomy have contributed significantly to the refinement of injection techniques for the treatment of dynamic forehead lines. A comprehensive assessment of eyebrow shape, position, and the aging process is essential, as the latter are closely linked to the functional balance between the frontalis muscle and the upper facial depressors. Optimal outcomes also depend on the accurate determination of dosage per injection point, injection depth, and strategic distribution of injection sites within the frontalis, which should be carefully considered and tailored to the individual’s anatomical characteristics and therapeutic goals—whether the aim is neuromodulation for muscle activity reduction or intradermal application for skin quality enhancement. A round table discussion session among three experienced international dermatology experts in aesthetic botulinum toxin type A was performed during a MERZ LATAM-sponsored medical education session. Recent insights in facial anatomy, including the precise location and distribution of motor endplates, as well as the direction of muscular force vectors during contraction; aging processes; and interindividual variability in facial musculature and mimicry patterns are discussed, and the results are described herein. These factors play a critical role in customizing personalized injection strategies and improving aesthetic outcomes in the treatment of forehead lines. Full article

Crack monitoring in concrete structures is essential to maintaining structural integrity. Therefore, this paper proposes a mobile ground robot equipped with a 2-DoF manipulator and stereo vision sensors for autonomous crack monitoring and mapping. To facilitate crack detection over large areas, a 2-DoF motorized manipulator providing linear and rotational motions, with a stereo vision sensor mounted on the end effector, was deployed. In combination with a manual rotation plate, this configuration enhances accessibility and expands the field of view for crack monitoring. Another stereo vision sensor, mounted at the front of the robot, was used to acquire point cloud data of the surrounding environment, enabling tasks such as SLAM (simultaneous localization and mapping), path planning and following, and obstacle avoidance. Cracks are detected and segmented using the deep learning algorithms YOLO (You Only Look Once) v6-s and SFNet (Semantic Flow Network), respectively. To enhance the performance of crack segmentation, synthetic image generation and preprocessing techniques, including cropping and scaling, were applied. The dimensions of cracks are calculated using point clouds filtered with the median absolute deviation method. To validate the performance of the proposed crack-monitoring and mapping method with the robot system, indoor experimental tests were performed. The experimental results confirmed that, in cases of divided imaging, the crack propagation direction was predicted, enabling robotic manipulation and division-point calculation. Subsequently, total crack length and width were calculated by combining reconstructed 3D point clouds from multiple frames, with a maximum relative error of 1%. Full article

Expandable polystyrene (EPS) nozzle covers can be used to replace traditional metal nozzle covers due to their excellent mechanical properties, as well as being lightweight and ablatable. As an important part of the solid rocket motor, the nozzle cover needs to be designed according to the requirements of the overall system. This study lays a theoretical foundation for the engineering design and performance optimization of the EPS nozzle cover. In this paper, the method of combining test research and numerical simulation is used to explore the pressure bearing capacity of EPS nozzle covers with different thicknesses under linear load. Firstly, the quasi-static tensile, compression and shear tests of EPS materials were carried out by universal testing machine, and the key parameters such as stress-strain curve, elastic modulus and yield strength were obtained; Based on the experimental data, the constitutive model of EPS material with respect to density is fitted and modified; The VUMAT subroutine of the material was written in Fortran language, and the mechanical properties of the nozzle cover with different material model distribution schemes and different thicknesses were explored by ABAQUS finite element numerical simulation technology. The results indicate that the EPS nozzle cover design based on the two material model allocation schemes better aligns with practical conditions; when the end thickness of the EPS nozzle cover exceeds 3 mm, the opening pressure formula for the cover based on the pure shear theory of thin-walled circular plates becomes inapplicable; the EPS nozzle cover exhibits excellent pressure-bearing capacity and performance, with its pressure-bearing capacity showing a positive correlation with its end thickness, and an EPS nozzle cover with a 9 mm end thickness can withstand a pressure of 7.58 MPa (under internal pressure conditions); the pressure-bearing capacity of the EPS nozzle cover under internal pressure conditions is higher than under external pressure conditions, and when the end pressure-bearing surface thickness increases to 9 mm, the internal pressure-bearing capacity is 3.13 MPa higher than under external pressure conditions. Full article

The problem of reducing electric power consumption is critical to ferrous metallurgy as it is a very energy-intensive industry. Significant energy savings can be achieved by increasing the efficiency of high-power electric drives of rolling mills. Experiments with the 5000 plate mill showed that the deterioration of energy efficiency can be caused by the misalignment of loads between the upper and lower roller main electric drive motors (upper main drive/UMD and lower main drive/LMD, respectively) caused by the misalignment of roller motor speeds. Experiments showed that when the speed misalignment reaches 5%, the motor torques differ by two times. Various UMD and LMD speeds can be set to bend the front end of the workpiece (form a “ski”). The installed load division controller (LDC) option fails to provide load alignment due to a low response rate and late startup. This article’s contribution consists of the development of a forced UMD and LMD speed and torque alignment method. To implement this method, a load-division controller with a switching structure has been developed. The authors also developed an efficiency and electric loss monitor and provided an experimental assessment of electric losses per one-pass and per sheet batch rolling cycle. The prospects of this research include the optimization of high-speed and high-load electric drive modes to reduce the energy costs of rolling and the development of an LDC based on fuzzy logic algorithms. Full article

Chemotherapy-induced peripheral neuropathy (CIPN) is a painful condition recalcitrant to current available therapies. CIPN pain can be severe and dose-limiting or dose-reducing for life-extending chemotherapeutics and, to date, there is no treatment to alter the progression of CIPN. For these experiments we used docetaxel, a first-line therapy for metastatic prostate cancer in humans and investigated the soluble epoxide hydrolase inhibitor EC5026 for its analgesic efficacy against this CIPN pain. Male SD rats (n = 10/group) were pretreated with 1 mg/kg EC5026 in formulated drinking water or vehicle for one week prior to docetaxel injections. The rats continued the formulated drinking water during three once-a-week docetaxel 10 mg/kg i.p. injections and were maintained on treatment until the end of week 5 when all groups were transitioned to normal drinking water. Nociceptive testing occurred throughout the entire experiment including after transitioning to normal drinking water. EC5026 increased mechanical withdrawal thresholds and latencies on the cold plate compared to docetaxel-treated controls. There were no motor effects of the compound, and the formulated drinking water provided favorable exposure. These results demonstrated that EC5026 administered prophylactically was both analgesic and able to limit the severity of mechanical and cold sensitivities in the docetaxel CIPN rat model. Full article

Autophagy impairments have been implicated in various aging conditions. Previous studies in cervical motor neurons show an age-dependent increase in the key autophagy proteins LC3 and p62, reflecting autophagy impairment and autophagosome accumulation. Chloroquine is commonly used to inhibit autophagy by preventing autophagosome–lysosome fusion and may thus emulate the effects of aging on the neuromuscular system. Indeed, acute chloroquine administration in old mice decreases maximal transdiaphragmatic pressure generation, consistent with aging effects. We hypothesized that chloroquine alters diaphragm muscle neuromuscular junction (NMJ) morphology and increases denervation. Adult male and female C57BL/6 × 129J mice between 5 and 8 months of age were used to examine diaphragm muscle NMJ morphology and denervation following daily intraperitoneal injections of chloroquine (10 mg/kg/d) or vehicle for 7 days. The motor end-plates and pre-synaptic terminals were fluorescently labeled with α-bungarotoxin and anti-synaptophysin, respectively. Confocal microscopy was used to assess pre- and post-synaptic morphology and denervation. At diaphragm NMJs, chloroquine treatment decreased pre-synaptic volume by 12% compared to the vehicle (p < 0.05), with no change in post-synaptic volume. Chloroquine treatment increased the proportion of partially denervated NMJs by 2.7-fold compared to vehicle treatment (p < 0.05). The morphological changes observed were similar to those previously reported in the diaphragm muscles of 18-month-old mice. These findings highlight the importance of autophagy in the maintenance of the structural properties at adult NMJs in vivo. Full article

Background and Clinical Significance: Congenital myasthenic syndrome-4C (CMS4C) associated with acetylcholine receptor (AChR) deficiency is an autosomal recessive defect of the motor endplate caused by homozygous or compound heterozygous mutations in the CHRNE gene on chromosome 17p13. Case Presentation: The authors present a familial case of CMS4C with three affected children in a consanguineous Romani family. Muscle weakness, fatigue, and ocular muscle impairment were present in all cases; two of the three siblings had delayed motor milestones, highly arched palates, and facial weakness. None of the children expressed bulbar symptoms. One child expressed a severe form, with recurrent respiratory infections, and multiple hospitalizations, while the other siblings expressed a mild phenotype, without hospital admissions. Repetitive nerve stimulation showed a myasthenic-type decrement greater than 10% of several muscles. A pathogenic frameshift variant (NM_000080.4: c.1327del) in the CHRNE gene was found in a homozygous status in all the affected children and in both parents. After 6 months of Pyridostigmine and Salbutamol treatment, the evolution of the case was good, with the improvement of most of the signs and no need for hospitalization. Conclusions: Early genetic diagnosis and appropriate therapy in the context of a multidisciplinary approach is mandatory for an optimal long-term prognosis. Community-wide carrier screening through comprehensive genetic testing is imperative to ensure accurate genetic counseling in genetic isolates. The authors report this case due to the increased number of affected children in a consanguine family from a small Romani community. Full article

Complex harvesting environments and varying crop conditions often lead to threshing cylinder blockage and increased entrainment loss in maize grain harvesters. To address these issues, an electric-driven automatic control system for maize threshing concave clearance based on real-time entrainment loss monitoring was developed. The system automatically adjusts concave clearance parameters at different harvesting speeds to maintain grain entrainment loss within an optimal range. First, an adjustable concave structure based on a crank-link mechanism was designed, with a threshing clearance adjustment range of 15–47 mm and motor rotation angle of 0–48°. Subsequently, an EDEM simulation model of the mixed material discharge inside the threshing cylinder was established to determine the optimal installation position of the entrainment loss monitoring sensor based on piezoelectric ceramic-sensitive elements. The sensor was positioned at the left tail end of the concave sieve, with a minimum distance of 58 mm between the sensitive plate centerline and threshing concave sieve and an installation angle of 65° relative to the horizontal plane. A maize threshing clearance control method based on fuzzy neural network PID control algorithm was proposed, and Simulink simulation optimization verified its superior performance with fast response speed. After system integration, field trials were conducted at low, medium, and high operating speeds with preset ideal entrainment loss intervals. The results showed that control was unnecessary at low speed, the control system-maintained entrainment loss within set range at medium speed, and maximum threshing clearance was needed at high speed. Finally, comparative trials of threshing performance with and without the control system were conducted at medium harvesting speed. Results showed that the entrainment loss rate decreased by 43.75% with the control system activated, significantly reducing maize threshing entrainment losses. This study overcame the barrier of maize threshing parameter adjustment being heavily reliant on manual experience and provided theoretical support for the intelligent grain harvesting equipment. Full article

Botulinum neurotoxin type-A (BoNT/A), which blocks quantal acetylcholine (ACh) release at the neuromuscular junction (NMJ), has demonstrated its efficacy in the symptomatic treatment of blepharospasm. In 3.89% of patients treated for blepharospasm at Tenon Hospital, BoNT/A was no longer effective in relieving the patient’s symptoms, and a partial upper myectomy of the Orbicularis oculi muscle was performed. We used surgical waste samples from 14 patients treated with repeated injections of either abobotulinumtoxinA (Dysport^®) or incobotulinumtoxinA (Xeomin^®). These muscle fragments were compared to others from 4 normal subjects, naïve of BoNT/A. The morphological study was performed blinded to the BoNT/A treatment and between treated and control samples. Neuromuscular specimens analyzed by confocal laser scanning microscopy, using fluorescent staining and immune-labeling of presynaptic proteins, revealed that the pattern of innervation (e.g., polyneuronal and convergent innervation), the muscle nicotinic ACh receptors (nAChRs), and the NMJs exhibited marked differences in BoNT/A-treated muscles (regardless of the toxin clinically used), with respect to controls. BoNT/A-treated junctions exhibited profuse polyneuronal innervation in which 2–6 axons innervated 74.84% of single muscle fibers, while 99.47% of control junctions were mono-innervated. Another new finding was the stable convergent innervation, in which several motor axons end onto the same endplate. Morphological signs of synapse elimination included the presence of retraction bulbs in axons and nerve terminals and a reduced extension of postsynaptic nAChRs. These outcomes suggest that synapse elimination is altered and raise questions on the origin and factors contributing to the plasticity changes observed and the functioning of NMJs. Full article

Age-related conditions, such as sarcopenia, cause physical disabilities for an increasing section of society. At the neuromuscular junction, the postsynaptic-derived neurotrophic factors brain-derived neurotrophic factor (BDNF) and neurotrophin 4 (NT-4) have neuroprotective functions and contribute to the correct regulation of the exocytotic machinery. Similarly, presynaptic muscarinic signalling plays a fundamental modulatory function in this synapse. However, whether or not these signalling pathways are compromised in ageing neuromuscular system has not yet been analysed. The present study analyses, through Western blotting, the differences in expression and activation of the main key proteins of the BDNF/NT-4 and muscarinic pathways related to neurotransmission in young versus ageing Extensor digitorum longus (EDL) rat muscles. The main results show an imbalance in several sections of these pathways: (i) a change in the stoichiometry of BDNF/NT-4, (ii) an imbalance of Tropomyosin-related kinase B receptor (TrkB)-FL/TrkB-T1 and neurotrophic receptor p 75 (p75^NTR), (iii) no changes in the cytosol/membrane distribution of phosphorylated downstream protein kinase C (PKC)βI and PKCε, (iv) a reduction in the M2-subtype muscarinic receptor and P/Q-subtype voltage-gated calcium channel, (v) an imbalance of phosphorylated mammalian uncoordinated-18-1 (Munc18-1) (S313) and synaptosomal-associated protein 25 (SNAP-25) (S187), and (vi) normal levels of molecules related to the management of acetylcholine (Ach). Based on this descriptive analysis, we hypothesise that these pathways can be adjusted to ensure neurotransmission rather than undergoing negative alterations caused by ageing. However, further studies are needed to assess this hypothetical suggestion. Our results contribute to the understanding of some previously described neuromuscular functional age-related impairments. Strategies to promote these signalling pathways could improve the neuromuscular physiology and quality of life of older people. Full article

There is a need for research addressing the functional characteristics of the motor end-plate in diabetes to identify mechanisms contributing to neuromuscular dysfunction. Here, we investigated the effect of diabetes on spontaneous acetylcholine release in the rat neuromuscular junction. We studied two randomized groups of male Wistar rats (n = 7 per group, 350 ± 50 g, 12–16 weeks of age): one with streptozotocin-induced experimental diabetes, and a healthy control group without diabetes. After 8 weeks of monitoring after diabetes induction, rats in both groups were anesthetized with pentobarbital. Then, the diaphragm muscle was dissected for electrophysiological recordings of miniature end-plate potentials (MEPPs) using a single electrode located at the region of the muscle end-plate. All experiments were conducted at environmental temperature (20–22 °C) in rat Ringer solution with constant bubbling carbogen (95% O₂, 5% CO₂). Compared to healthy controls, in the diaphragm neuromuscular end-plate derived from diabetic rats, the MEPPs were higher in amplitude and frequency, and the proportion of giant MEPPs was elevated (7.09% vs. 1.4% in controls). Our results showed that diabetes affected the acetylcholine MEPP pattern and increased the number of giant potentials compared to healthy controls. Full article

Neuromuscular disorders (NMDs) encompass a highly diverse group of conditions that affect the skeletal muscles, peripheral nervous system, or motor endplate. Depending on the underlying disease, common characteristics include progressive muscle weakness and sensory disturbances, both of which can contribute to sleep disruption. Disorders of sleep are extremely frequent in NMDs and substantially co-determine overall morbidity, quality of life, and survival. As many NMDs currently lack a cure, supportive therapy is mandatory and includes appropriate management of sleep-related symptoms. Specific sleep disorders that may arise in NMDs include insomnia due to pain or leg muscle cramps, restless legs syndrome, and sleep-disordered breathing, notably obstructive sleep apnea and hypoventilation. This review article aims to comprehensively outline the clinical spectrum of sleep disorders and sleep properties associated with NMDs. Full article

Amyotrophic lateral sclerosis (ALS) is manifested as skeletal muscle denervation, loss of motor neurons and finally severe respiratory failure. Mutations of RNA-binding protein FUS are one of the common genetic reasons of ALS accompanied by a ‘dying back’ type of degeneration. Using fluorescent approaches and microelectrode recordings, the early structural and functional alterations in diaphragm neuromuscular junctions (NMJs) were studied in mutant FUS mice at the pre-onset stage. Lipid peroxidation and decreased staining with a lipid raft marker were found in the mutant mice. Despite the preservation of the end-plate structure, immunolabeling revealed an increase in levels of presynaptic proteins, SNAP-25 and synapsin 1. The latter can restrain Ca²⁺-dependent synaptic vesicle mobilization. Indeed, neurotransmitter release upon intense nerve stimulation and its recovery after tetanus and compensatory synaptic vesicle endocytosis were markedly depressed in FUS mice. There was a trend to attenuation of axonal [Ca²⁺]_in increase upon nerve stimulation at 20 Hz. However, no changes in neurotransmitter release and the intraterminal Ca²⁺ transient in response to low frequency stimulation or in quantal content and the synchrony of neurotransmitter release at low levels of external Ca²⁺ were detected. At a later stage, shrinking and fragmentation of end plates together with a decrease in presynaptic protein expression and disturbance of the neurotransmitter release timing occurred. Overall, suppression of synaptic vesicle exo–endocytosis upon intense activity probably due to alterations in membrane properties, synapsin 1 levels and Ca²⁺ kinetics could be an early sign of nascent NMJ pathology, which leads to neuromuscular contact disorganization. Full article

The authors propose a technique for reactive power compensation using a powerful regenerative controlled-speed synchronous motor drive (SMD) based on a three-level (3L) neutral point clamped (NPC) active front-end rectifier (AFE) and a voltage source inverter (VSI). The review of technical solutions for reactive power compensation showed that the limitations on the transmitted reactive power in the system under consideration still have not been studied. The paper provides a mathematical description and proposes synthesis-friendly block diagrams of the mathematical 3L-NPC-AFE-VSI and SMD models. The developed models allow defining the instantaneous values of the total 3L-NPC-AFE power consumed from the grid depending on the SMD load diagram. It is noted that the 3L-NPC-AFE-VSI-SMD system is designed without considering the opportunities for reactive power generation. It was determined that the limit value of reactive power generated by a 3L-NPC-AFE depends on the DC link voltage, the grid current consumption and the modulation index. The possibility of reactive power compensation by the SMD system through a 3L-NPC-AFE was experimentally tested on the main drive of a metal plate hot rolling mill. The analysis of the results obtained showed that during the breakdown, an SMD can generate reactive power equal to 16% of the total rated power using a 3L-NPC-AFE at a rated DC link voltage and without overcurrent. It was shown that generating reactive power is expedient in low-load SMD operation modes or at idle. Research in this area is promising due to the widespread use of high-power SMD based on a 3L-NPC-AFE-VSI and the tightening of requirements for energy saving and efficiency and supply voltage quality. The proposed reactive power control technique can be used as part of an industrial smart grid. Full article