Search Results (447)

In the context of global population aging, identifying reliable, objective tools to assess physical function and postural stability in older adults is increasingly important to mitigate fall risk. This study presents a non-contact platform that uses a Microsoft Azure Kinect depth camera to evaluate functional performance related to lower-limb muscular capacity and static balance through self-selected depth squats and four progressively challenging stances (feet apart, feet together, semitandem, and tandem). By applying markerless motion capture algorithms, the system provides key biomechanical parameters such as center of mass displacement, knee angles, and sway trajectories. A comparison of older and younger individuals showed that the older group tended to perform shallower squats and exhibit greater mediolateral and anteroposterior sway, aligning with age-related declines in strength and postural control. Longitudinal tracking also illustrated how performance varied following a fall, indicating potential for ongoing risk assessment. Notably, in 30 s balance trials, the first 10 s often captured meaningful differences in stability, suggesting that short-duration stance tests can reliably detect early signs of imbalance. These findings highlight the feasibility of low-cost, user-friendly depth-camera technologies to complement traditional clinical measures and guide targeted fall-prevention strategies in older populations. Full article

Muscular strength is an essential factor in sports performance and general health, especially for optimizing mechanical power, as well as for injury prevention. The present study biomechanically characterized the half squat (HS) using a systemic structural approach based on mechanical power, called Power-Based Training (PBT), through which four phases of the movement were determined (acceleration and deceleration of lowering and lifting). Five weightlifters from the Mexican national team (categories U17, U20, and U23) participated, who performed five repetitions per set of HS with progressive loads (20%, 35%, 50%, 65%, and 80% of the one repetition maximum). The behavior of the center of mass of the subject–bar system was recorded by photogrammetry, calculating position, velocity, acceleration, mechanical power, and mechanical work. The results showed a significant reduction in velocity, acceleration, and mechanical power as the load increases, as well as variations in the duration and range of displacement per phase. These findings highlight the importance of a detailed analysis to understand the neuromuscular demands of HS and to optimize its application. The PBT approach and global center of mass analysis provide a more accurate view of the mechanics of this exercise, facilitating its application in future research, as well as in performance planning and monitoring. Full article

A high-fat diet (HFD) can lead to obesity and skeletal muscle atrophy. Sleeve gastrectomy (SG) improves obesity and increases skeletal muscle mass. This study examined whether SG prevented skeletal muscle atrophy in a diet-induced rat obesity rat model. First, 8-week-old male Sprague-Dawley rats underwent surgical (sham-operated or SG) and dietary (standard, high-fat diet, or same pair feeding as SG [PF]) interventions without exercise. In the second experiment, treadmill exercise was added for 4 weeks post-SG (SG + Ex). In the third experiment, rats received an adiponectin receptor agonist (AdipoRon) injection. The HFD induced weight gain and decreased muscle fiber area. SG + Ex reversed these levels, followed by increases in adiponectin in the blood and skeletal muscle and myoblast determination protein 1 (MyoD) and decreased peri-muscular adipose tissue (PMAT) mass, but SG alone did not. No similar changes were observed in the PF group, with or without exercise. Injection of AdipoRon had a similar effect on skeletal muscle and PMAT as SG + Ex. The combination of SG and exercise, but not calorie restriction alone, had better impacts on skeletal muscle and PMAT than SG or exercise alone. Full article

Background/Objectives: During adolescence, the critical growth period, the antioxidant selenium (Se), either as sodium selenite or selenium nanoparticles (SeNPs), has shown contrasting effects on adipose tissue (AT) in rats, due to its role in insulin signaling. Since skeletal muscle (SKM) is also a key insulin-target tissue, this study aimed to assess whether a similar effect occurs in this tissue. Methods: Three groups of male adolescent rats (n = 18) were used: control (C), selenite supplemented (S), and SeNPs supplemented (NS). Low doses of Se were administered via drinking water in both supplemented groups. AT was utilized for transcriptomic analyses, while SKM was analyzed for oxidative balance, insulin-induced anabolic effects, and proteolysis. Myokine levels in serum were also determined. Results: SeNPs administration decreased SKM mass and protein content, increased serum creatinine, and decreased insulin levels, indicating impaired SKM development. Both supplemented groups upregulated genes related to creatine metabolism and muscle contraction. However, only the NS group showed upregulation of genes associated with glycogenolysis and glycolysis. Despite unchanged GPx1 expression, NS rats presented lower oxidation and insulin–pmTOR activation, and higher expression of proteins related to proteolysis (pAMPK, SIRT1, ULK1, FOXO3a, and MaFbx) and a myokine profile compatible to muscle atrophy, fatty acid oxidation, and impaired myoblast proliferation. Ultimately, the selenite group impaired SKM catabolism mainly by increasing insulin–pmTOR activation. Conclusions: Once again, the form of Se administration exerts opposing effects on metabolism tissues, suggests a potential therapeutic role for selenite in disorders that compromise muscle growth, such as muscular dystrophies, cachexia, or sarcopenia. Full article

Hepatic sarcomas are rare and aggressive tumors in veterinary medicine, with limited reports in the literature. This case report describes a canine hepatic myofibroblastic sarcoma in a 5-year-old spayed female Dobermann. The dog presented with abdominal enlargement and was diagnosed with a large hepatic mass following comprehensive diagnostic evaluations, including blood tests, imaging, and histopathology. Histological and immunohistochemical analyses confirmed the tumor’s myofibroblastic origin, characterized by positivity for markers such as vimentin, glial fibrillary acidic protein (GFAP), nerve growth factor receptor (NGFR), alpha-smooth muscle actin (SMA), and muscular actin (HHF35). Treatment involved a combination of intense-dose chemotherapy using doxorubicin and a subsequent metronomic chemotherapy protocol, which resulted in prolonged survival of over 690 days at the time this manuscript was written. This case highlights the importance of extensive diagnostic and immunohistochemical profiling in the accurate classification of and treatment planning for hepatic sarcomas, and emphasizes the role of advanced veterinary diagnostics in improving patient outcomes. Future studies with larger sample sizes are needed to enhance understanding of the biological behavior and optimal therapeutic strategies for such rare tumors. Full article

Sarcopenic obesity (SO), characterized by an excess of fat and a decrease in muscle strength or mass, is a global public health concern and is linked to metabolic conditions such as metabolic syndrome (MetS). Different mechanisms contribute to SO, such as inflammation, fatty acid infiltration, and insulin resistance (IR). Recently, myostatin (MYOST), an inhibitory factor for skeletal muscle tissue, was proposed as an aimed compound for the treatment of conditions of muscular metabolic imbalance mass and MetS. On the other hand, a therapy with natural compounds such as resveratrol (R) and quercetin (Q) is effective for the treatment of MetS, but its effect on the MYOST pathway has been poorly explored. The control group received water, and the MetS group received 30% commercial sugar in the drinking water for 6 months. Polyphenol mix (R at a dose of 50 mg/kg/day and Q at 0.95 mg/kg/day) was administered for 1 month. MetS rats present SO linked to an increase in the expression of MYOST/ActRIIA and ActRIIB (p < 0.0001). R+Q treatment prevented SO by lowering the expression of MYOST and its receptors and increased the expression of Smad 7 in MetS rats (p < 0.0001). Moreover, the polyphenol treatment reverted IR by increasing Akt phosphorylation, leading to an increase in muscle mass. It decreased lipid stores, restored glycogen accumulation, and increased myosin expression (p < 0.0001). The results of this work indicate that R+Q supplementation could be a promising therapeutic agent to prevent SO and sarcopenia derived from other metabolic alterations. Full article

This study aims to evaluate the effects of a Walking Football (WF) programme on physical fitness, body composition, health-related quality of life (QoL), and happiness in men and women over 60 years. WF is proposed as a safe and accessible activity to counteract physical inactivity and chronic conditions in ageing populations. A randomized controlled trial will be conducted with 120 participants (both sexes), assigned to either an experimental group (WF intervention) or a wait-list control group. The six-month intervention includes bi-weekly sessions with skills training, match simulations, and fitness exercises. Assessments will be performed at baseline, 3 months, and 6 months. Primary outcome measures will be: body composition (weight, BMI, fat and lean mass), muscular strength, aerobic endurance, agility, range of motion, and subjective well-being (QoL and happiness scales). Linear mixed-effects models and ANCOVA will be used to analyse the data. It is expected that WF will lead to improvements in physical and mental health, contributing to active ageing. This study may also support WF as a tool for social inclusion and well-being in older adults. Full article

Objectives: This study aimed to compare the acute responses of the muscular activity of primary movers during bench press execution under asymmetric loads (25%, 50%, and 75%). Methods: The study included 30 resistance-trained males (n = 25, age = 22.73 ± 3.44 years, height= 1.77 ± 0.06 m, body mass= 76.77 ± 9.28 kg) and females (n = 5, age = 22.5 ± 1.19 years, height = 1.63 ± 0.04 m, body mass = 56.78 ± 2.90 kg). We assessed the two portions of the dominant pectoralis major, triceps brachii, anterior deltoid, and both external oblique peak activities (sEMG) during concentric and eccentric phases. We performed a repeated-measures design to establish the differences between muscle activity, barbell center of mass acceleration, and OMNI-Perceived Exertion Scale for Resistance Exercise (OMNI-RES) in a bench press under seven different conditions. Results: The linear mixed model showed a significant fixed effect for exercise condition for muscles (p < 0.001) in the concentric and eccentric phases. We found significantly higher clavicularis (d = 0.54; d = 1.15) and sternalis (d = 0.38; d = 0.86) pectoralis major activation of the dominant side under high (50% and 75%), non-dominant-side, de-loaded conditions in the eccentric phase (p < 0.001), with large effects. Contralateral core muscles (external oblique) of the dominant and non-dominant sides were significantly (p < 0.001) highly activated under all asymmetric conditions in the concentric phase (from d = 0.89 to d = 2.30). Conclusions: The asymmetric load bench press provoked a higher pectoralis major activation on the loaded side when de-loading the other side. The contralateral external oblique doubles the muscle activity in the most asymmetric conditions. Full article

Objective: This study analyzed the relationship between self-perceived physical fitness and anthropometric and biochemical variables in young athletes from extracurricular sports programs in northeastern Spain. Methods: A cross-sectional design was used with a sample of 673 young athletes. Data collection included self-reported physical fitness and objective anthropometric and biochemical measurements. The analysis explored associations between perceived fitness dimensions and physical/biochemical variables, with attention to sex differences. Results: Fat mass showed significant inverse associations with all perceived fitness dimensions: general fitness (OR = 0.62, 95% CI [0.41, 0.94]), cardiorespiratory fitness (OR = 0.56, 95% CI [0.37, 0.83]), muscular strength (OR = 0.61, 95% CI [0.41, 0.91]), speed/agility (OR = 0.59, 95% CI [0.39, 0.88]), and flexibility (OR = 0.57, 95% CI [0.39, 0.84]). Higher fat mass was consistently linked to lower perceived fitness. HDL levels were positively associated with general (OR = 1.40, 95% CI [1.13, 1.74]) and cardiorespiratory fitness (OR = 1.32, 95% CI [1.07, 1.62]), while LDL levels showed no significant effect (p > 0.05). Sex differences emerged for general fitness (OR = 0.52, 95% CI [0.33, 0.82]) and flexibility (OR = 0.51, 95% CI [0.33, 0.78]), favoring boys, but no differences were found for cardiorespiratory fitness, muscular strength, or speed/agility (p > 0.05). This suggests that shared athletic environments may reduce typical sex-based disparities. Conclusions: Our findings emphasize the importance of considering both anthropometric and biochemical variables when evaluating perceived fitness in youth athletes. Regular athletic engagement may buffer sex-based differences in fitness perception. Full article

Background: Malnutrition, influenced by inflammation, is associated with muscle depletion and body composition changes. This study aimed to evaluate muscle mass and quality using Artificial Intelligence (AI)-enhanced ultrasonography in patients with inflammation. Methods: This observational, cross-sectional study included 502 malnourished patients, assessed through anthropometry, electrical bioimpedanciometry, and ultrasonography of the quadriceps rectus femoris (QRF). AI-assisted ultrasonography was used to segment regions of interest (ROI) from transversal QRF images to measure muscle thickness (RFMT) and area (RFMA), while a Multi-Otsu algorithm was used to extract biomarkers for muscle mass (MiT) and fat mass (FatiT). Inflammation was defined as C-reactive protein (CRP) levels above 3 mg/L. Results: The results showed a mean patient age of 63.72 (15.95) years, with malnutrition present in 82.3% and inflammation in 44.8%. Oncological diseases were prevalent (46.8%). The 44.8% of patients with inflammation (CRP > 3) exhibited reduced RFMA (2.91 (1.11) vs. 3.20 (1.19) cm², p < 0.01) and RFMT (0.94 (0.28) vs. 1.01 (0.30) cm, p < 0.01). Muscle quality was reduced, with lower MiT (45.32 (9.98%) vs. 49.10 (1.22%), p < 0.01) and higher FatiT (40.03 (6.72%) vs. 37.58 (5.63%), p < 0.01). Adjusted for age and sex, inflammation increased the risks of low muscle area (OR = 1.59, CI: 1.10–2.31), low MiT (OR = 1.49, CI: 1.04–2.15), and high FatiT (OR = 1.44, CI: 1.00–2.06). Conclusions: AI-assisted ultrasonography revealed that malnourished patients with inflammation had reduced muscle area, thickness, and quality (higher fat content and lower muscle percentage). Elevated inflammation levels were associated with increased risks of poor muscle metrics. Future research should focus on exploring the impact of inflammation on muscles across various patient groups and developing AI-driven biomarkers to enhance the diagnosis, monitoring, and treatment of malnutrition and sarcopenia. Full article

Background/Objectives: Structured medium- and long-chain triglycerides (sMLCT) may be a superior vehicle for medium-chain fatty acid delivery to peripheral tissues, such as skeletal muscle. Limited information is available concerning the effect of sMLCT on muscular performance or recovery after a muscle-damaging exercise protocol. The purpose of this study was to establish the effect of a novel formulation of sMLCT on muscular performance and recovery. Methods: Forty female adults (mean ± SD age = 22 ± 3 years; body mass index = 23.5 ± 3.4 kg/m²) were randomized into one of two study groups, placebo control [CON; n = 20] or sMLCT [n = 20], and completed five total visits to the laboratory. The baseline (i.e., pre-exercise) assessments of muscle performance, size, and soreness were compared to assessments immediately following exercise and 24, 48, and 72 h post-exercise. Results: No statistically significant condition × time interactions were noted for strength outcomes, although trends for condition × time interactions were present for torque over 25 ms (p = 0.06) and peak torque (p = 0.05). Similarly, no condition x time interactions were present for ultrasound echo intensity, the subjective ratings of soreness and pain, thigh circumference, leg volume, and vertical jump performance. Conclusions: Within the context of the current study, the ingestion of sMLCT did not significantly influence the rate of muscle strength recovery following muscle damaging resistance exercise. Full article

Background/Objectives: Recent interest has emerged in novel initiatives that focus on the “whole child” to improve the health and well-being in youth. The purpose of this study was to determine whether a translational health in nutrition and kinesiology (THINK) program addressing physical, psychological, and educational well-being could improve personal health and lifestyle behaviors in youth. Methods: A total of 81 adolescents (44 males, 37 females, 12.50 + 0.62 years) were evaluated at the beginning and end of the spring semester across three different years: 2019, 2021, and 2023. The physical literacy measures included the Physical Activity Enjoyment Scale (PAES) and the Physical Activity Perception Scale (PAPS), along with knowledge-based tests in nutrition and exercise science. Social emotional learning (SEL), STEM education, and nutrition habits were evaluated using standard surveys and questionnaires. The physical evaluations included measures of body composition and physical fitness. Results: At the completion of the spring semester of each year, evaluations were compiled for all three years, with decreases found for % body fat (p < 0.001) and increases found for lean body mass (p < 0.001). The physical fitness components, including muscular strength (p < 0.001), cardiorespiratory fitness (p < 0.001), power (p < 0.001), and flexibility (p < 0.01), all improved. The physical literacy components, including the PACES (p < 0.001), PAPS (p < 0.001), exercise (p < 0.05), and nutrition science knowledge (p < 0.05) results, also improved. There were no significant changes in any other variables. Conclusions: A novel in-school academic curriculum integrating the physical, psychological, and educational well-being of the whole child could lead to improved body composition, physical fitness, and physical literacy. Full article

A 78-year-old woman was referred to our hospital for close examination of an extramural submucosal tumor in the gastroesophageal region, suspected based on an imaging test performed for a chief complaint of epicardial pain while eating. Contrast-enhanced computed tomography revealed a 3 cm sized mass with well-defined margins and a homogeneous interior near the gastroesophageal junction. Endoscopic ultrasonography (EUS) revealed a large (28 mm) unilocular cystic lesion with a heterogeneous hypoechoic internal structure. The cyst wall was layered with a hypoechoic layer that appeared to be muscular and continuous with the external longitudinal muscle of the esophagus. Based on the EUS findings, an esophageal duplication cyst was diagnosed. Cystectomy was performed because the patient was symptomatic. Pathological examination revealed that the specimen was covered with columnar and pseudostratified ciliated epithelium without atypia and that the cyst wall comprised two layers of smooth muscle. No cartilaginous tissue was present, which is consistent with esophageal duplication cysts. Retrospectively, the EUS findings were consistent with the pathological findings. Full article

Objectives: Handgrip strength (HGS) has been used as an important health indicator due to its ability to predict morbidity and mortality risks in various populations. The aim was to investigate the relationship between HGS and health parameters among public sector workers. Methods: A cross-sectional study was carried out, including the participation of 129 workers. The body composition, presence of chronic non-communicable diseases (CNCDs), physical activity level, and physical capacity (flexibility, HGS, and lower limb endurance) of the workers were evaluated. Results: The results showed that most of the employees, regardless of sex, work in the administrative sector, are aged 40 years or older, have chronic non-communicable diseases (CNCDs), and are physically inactive. The majority of women have a body mass index (BMI) within the normal range and are more flexible. On the other hand, men demonstrated greater lower limb endurance. Lower HGS was associated with a higher BMI, the presence of a CNCD, and worse lower limb endurance. A negative correlation was observed between HGS and age, BMI, and body fat. Conversely, a positive correlation was found between HGS and muscle mass, as well as with lower limb muscle endurance. Conclusions: It can be concluded that individuals with lower HGS are more likely to have a high BMI, develop a CNCD, and present worse lower muscular endurance. Additionally, HGS was found to be reduced in older individuals and/or those with higher levels of body fat, and to be higher in those with normal physical capacity and body composition. Full article

Background: We established a model of diet-induced obesity in Sprague–Dawley rats that produces, in addition to obesity, metabolic syndrome and musculoskeletal degeneration. Prebiotic fiber and aerobic exercise interventions have been shown to rescue bones and joints from degeneration, but it has yet to be shown if muscle degeneration can also be stopped with these interventions. Objectives: This study was aimed at determining if prebiotic fiber supplementation and/or aerobic exercise can prevent muscular alterations in our rat model of obesity. Methods: Using a high-fat/high-sucrose (HFS) diet-induced rat model of obesity, 12-week-old male Sprague–Dawley rats were randomly divided into sedentary (HFS, n = 12), exercise (HFS + E, n = 12), prebiotic fiber supplementation (HFS + F, n = 12), or combined intervention (HFS + F + E, n = 12) groups for 12 weeks, with eight chow-fed animals as controls. Muscle triglyceride levels were measured using colorimetric assays, collagen content was assessed histologically, and CD68 immunohistochemistry was performed on the vastus lateralis (VL) and soleus muscles. Group comparisons were conducted using the Kruskal–Wallis test and chi-squared effect statistics (χ2). Results: VL triglyceride (χ2 = 10.481, p = 0.033) and collagen content in both VL and soleus (χ2 = 23.148, p < 0.001 and χ2 = 34.166, p < 0.001 respectively) were higher in all HFS-diet intervention groups compared to the chow-fed Control group. Lean body mass did not differ among groups (χ2 = 3.9192, p = 0.417). The HFS group exhibited increased total cholesterol and triglyceride levels (χ2 = 11.693, p = 0.019; and χ2 = 21.663, p < 0.001 respectively) and starkly reduced whole-body insulin sensitivity (χ2 = 18.046, p = 0.001) compared to the Control or to the exercise and fiber supplementation groups. Conclusions: Despite the effectiveness of aerobic exercise and prebiotic fiber supplementation in preventing the systemic metabolic disturbances induced by the HFS diet, muscular alterations persisted. Prebiotic fiber supplementation led to the highest muscle collagen content, suggesting potential adaptative muscular response to the systemic insult caused by the HFS diet. Full article