Search Results (33,360)

The rural landscape, as the core constituent unit of rural space, is vital for promoting sustainable rural development and achieving rural revitalization goals. However, current research is often limited to single groups, neglecting differences in visual behaviors (VBs) and landscape preferences (LPs) among diverse groups. Thus, this study employed eye-tracking technology combined with a landscape preference questionnaire to investigate the VBs and LPs of 160 participants when viewing rural landscapes. The results indicate that there is a notable correlation between VBs and LPs (p < 0.01), and the two aspects mutually influence each other. Moreover, groups with different demographic characteristics exhibit significant differences in both VBs and LPs. Among them, the score for LPs in the group of farmers, service sector workers, and retirees is significantly higher than that in the group of students, while the mean fixation duration is significantly lower than that in the group of students. Based on these research findings, this study suggests that, during the process of rural landscape design, full consideration should be given to the VBs and LPs of diverse groups with different demographic characteristics to enhance the inclusivity of rural landscape design and facilitate the realization of diversified and sustainable rural development. Full article

Plants grow, develop, and reproduce within a rhythmic environment. Environmental cues—such as light, temperature, nutrition, water—initiate, sustain, or terminate basic physiological processes within the plant, such as photosynthesis, respiration, nutrient uptake, water management, transpiration, growth, and hormone regulation. Simultaneously, inside the plant, internal “living clocks” are ticking and helping plants to synchronize internal processes with environmental cues and defend themselves against stressful conditions. These clock-regulated processes underlie a variety of plant traits, such as germination capability, growth and development rate, time of flowering, fruiting and yielding, development of plant shape, and size and biomass production. Most of these physiological traits are important attributes of crop plants. In recent years, the growing understanding of environmental rhythms as environmental cues and the mechanisms underlying plant internal clocks has begun to play an increasingly important role in agricultural practices. This is an emerging area of research that integrates insights from chronobiology with practices in plant agriculture. In this review, this new research area is studied and mapped using Scopus, Web of Science, Google Scholar, Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA protocol), and VOSviewer1.6.20 software. The analyses were carried out on 18 July–27 August 2025. For the VOSviewer author keywords co-occurrence analysis, all 1022 documents covering the time range of the last 7.5–2.5 years (2018–July 2025) were included and three maps were generated. Additionally, 59 review documents covering the last 27 years (1988–July 2025) were extracted by relevance using Google Scholar. In this review, recent advances and topics in plant chronobiology were examined. The issue of how these advances respond to key challenges in plant agriculture was explored. The bidirectional influence between chronobiology and practices in plant agriculture were also considered. Full article

With the maturity of haptic technology and complex systems, tactile interaction has gradually become realized through specific hardware and software configurations in the e-commerce and business industries. As an innovative form depending on haptic systems, tactile interactive advertising could help both advertisers and consumers enhance the haptic experience of products through technology-mediated virtual environments and provide tactile information for purchase decision making that relies on restoring the real sense of touch. On the basis of the value-based adoption model (VAM) and the need for touch (NFT) from a preference for haptic information in a system, we conduct quantitative research and construct a partial least squares structural equation model, which aims to study the influencing factors that characterize the user preference of tactile interactive advertisements empowered by haptic systems among Chinese consumers. A total of 509 valid questionnaires were collected through online and offline channels. The study revealed that the perceived enjoyment (PE) and telepresence (TEL) of tactile interactive advertisements as benefit factors positively influence the perceived value (PV) and that the perceived fee (PF) as a sacrifice factor negatively influences PV, which further impacts the attitude and intention to use (IU). In addition, the study verified that a higher NFT positively affected PE, PU, and PF and IU for the perception of tactile interactive advertising. Through this study, we aim to provide insights from a consumer perspective to enhance the advertising effect and user experience through tactile interaction in further e-commerce, which transforms how we interact with digital systems and virtual environments. Full article

Background/Objectives: Survey administration mode may significantly influence responses on polarizing health topics, yet this methodological factor remains understudied in vaccine hesitancy research. Understanding how data collection methods affect parental attitudes toward childhood vaccination is crucial for accurate public health surveillance and intervention design. Methods: This comparative cross-sectional study examined parental attitudes toward childhood vaccination using both paper-based (n = 487) and online (n = 386) survey administration among 873 parents. This study employed multivariate logistic regression analysis to assess differences between survey modes while controlling for demographic variables. Results: Key outcomes included general vaccination support, belief in vaccine–autism links, preference for natural immunity, and communication comfort with healthcare providers. Substantial differences emerged between survey modes. Online respondents showed significantly lower vaccination support (61.92% vs. 88.48%, p < 0.001), higher belief in the vaccine–autism link (37.31% vs. 16.77%, p < 0.001), and greater endorsement of natural immunity over vaccination (38.08% vs. 12.50%, p < 0.001). After adjusting for demographics, online respondents had 5 times lower odds of supporting childhood vaccination (OR = 0.20, 95% CI: 0.13–0.30) and nearly 5 times higher odds of preferring natural immunity (OR = 4.67, 95% CI: 3.19–6.95). Online respondents were also less likely to feel comfortable discussing vaccines with healthcare providers (51.0% vs. 72.7%, p < 0.001). Conclusions: Survey administration mode substantially influences measured parental vaccine attitudes, with online platforms capturing more vaccine-skeptical responses. These findings have critical implications for public health research methodology and suggest that mixed-mode survey designs or statistical adjustments may be necessary to obtain representative population estimates of vaccine hesitancy. Full article

Background and objective: Minimally invasive interventions, including irreversible electroporation (IRE), cryoablation, and high-intensity focused ultrasound (HIFU), offer promising alternatives for the treatment of low- and intermediate-risk prostate cancer. We aimed to evaluate the oncological efficacy and safety of these treatments. Methods: A systematic search of MEDLINE, Central, and EMBASE was conducted up to 5 January 2025, according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Recurrence, complication, survival, biochemical, and retreatment rates were evaluated, with risk of bias assessed using the Risk of Bias In Non-randomized Studies of Interventions (ROBINS-I) and Risk of Bias 2 (RoB2) tools. Results: 85 studies met the inclusion criteria, comprising 42 prospective cohort studies, 36 retrospective cohort studies, six registries, and one randomized controlled trial. Whole-gland HIFU showed significantly lower recurrence (15%) and postoperative mean PSA levels (0.68 ng/mL) than focal HIFU (24%, 2.81 ng/mL). Recurrence rates were similar for focal vs. extended IRE (30% vs. 26%) and focal vs. whole-gland cryoablation (18% vs. 13%). In-field and out-of-field recurrence rates were similar across treatment modalities (5–15%). Retreatment rates were low, with 6–7% of patients receiving a second ablation and 2–8% progressing to radical or hormonal therapy. Major complications were consistently rare. One-year biochemical recurrence-free survival (BRFS) exceeded 95%, and five-year BRFS approached 80% for HIFU and cryoablation. Conclusions: Minimally invasive focal and whole-gland therapies are safe and effective for treating low- and intermediate-risk prostate cancer, with high survival and low major complication rates. Notably, whole-gland HIFU achieves superior biochemical control and lower recurrence than focal HIFU, emphasizing the clinical importance of treatment extent. Full article

Pancreatic pseudocysts (PPCs) are frequent complications of acute and chronic pancreatitis, characterized by encapsulated collections of pancreatic fluid. Historically managed by open surgical approaches, treatment paradigms have significantly evolved with advancements in imaging and minimally invasive techniques. This review outlines the historical progression and current standards in PPC management, covering conservative, surgical, laparoscopic, and endoscopic interventions. Conservative management remains a valid first-line option for asymptomatic, stable pseudocysts, particularly in the absence of complications. Surgical techniques, once the mainstay, such as marsupialization and internal drainage procedures (cystogastrostomy, cystojejunostomy, and cystoduodenostomy), now serve as alternatives when less invasive methods fail. Laparoscopic approaches offer reduced morbidity and faster recovery, especially for complex or inaccessible PPCs. However, endoscopic drainage, particularly endoscopic ultrasound-guided transmural drainage using plastic or metal stents—especially lumen-apposing metal stents (LAMSs)—has become the preferred modality due to its efficacy, safety profile, and cost effectiveness. Emerging technologies, including robotic-assisted surgery and hybrid techniques, promise further refinement in PPC management. This review synthesizes current evidence and expert guidelines, providing a comprehensive overview of evolving strategies and future directions in the treatment of PPCs. Full article

Urban-to-rural migration is reshaping vernacular villages through transformations in both architectural form and everyday life. This study focuses on three villages in Zhejiang Province, China, and their migrants from urban areas, investigating—through field surveys and interviews—how urban-to-rural migrants’ spatial and lifestyle interventions influence the preservation and transformation of traditional architecture and local cultural practices. Findings indicate that urban-to-rural migrants exhibit diverse spatial preferences and lifestyle patterns, leading to varied modes of building adaptation. Some prioritize modern styles and commercial functions, while others emphasize cultural continuity, community engagement, or individual expression. Most buildings undergo incremental modifications rather than complete reconstruction, reflecting a balance among regulatory constraints, financial considerations, and personal aspirations. Furthermore, some migrants retain traditional spatial hierarchies and layout logic in their architectural designs, thereby sustaining vernacular lifestyles such as intergenerational cohabitation and neighborhood interaction. These building practices also have demonstrative effects within the village, encouraging others to value local culture and spatial traditions. In contrast, other migrants, driven by modern aesthetics or commercial objectives, restructure or even disrupt traditional spatial models, resulting in the fragmentation and weakening of established value systems. These insights contribute to a deeper understanding of how urban-to-rural migration reshapes the spatial organization of traditional villages and can inform more flexible and context-sensitive rural planning practices. Full article

This study investigates the factors influencing green purchase behavior in emerging economies, focusing on Indonesian consumers’ preferences for organic food products. While sustainability awareness is growing globally, limited research has examined how environmental knowledge and trust interact with marketing efforts to shape green purchasing decisions in developing market contexts like Indonesia. The research model incorporates five constructs: environmental knowledge (awareness of ecological issues), green trust (confidence in environmental claims), green marketing mix (eco-oriented strategies for product, price, place, and promotion), green purchase intention (likelihood of buying eco-friendly products), and green purchase behavior (actual sustainable buying decisions). Data from 211 valid respondents were analyzed using structural equation modeling. The results indicate that environmental knowledge directly influences green trust and the green marketing mix but not green purchase intention or behavior. Instead, it affects behavior indirectly through trust and intention. Contrary to expectations, green trust does not influence the green marketing mix, suggesting it may operate independently of marketing strategies. Similarly, the green marketing mix does not significantly influence green purchase intention or behavior, suggesting that marketing strategies alone may be insufficient in driving sustainable consumer choices. These findings highlight the important role of environmental knowledge in fostering consumer trust and indirectly guiding green purchasing behavior in emerging markets. By promoting sustainable consumption through knowledge and trust, this study offers insights into consumer behavior as a pathway to advancing planetary health. This study advances the Theory of Planned Behavior by integrating green trust and the green marketing mix to explain how trust and economic factors shape green purchasing behavior. Practical implications suggest that businesses should adopt targeted green marketing strategies, such as educational campaigns, eco-labeling, or certifications, to enhance environmental awareness, build consumer trust, and encourage sustainable purchasing decisions. This study contributes to the literature by examining how environmental knowledge indirectly influences green purchase behavior through the mediation of trust and intention within the context of an emerging market. Full article

Background/Objectives: Radiotherapy can cause severe and irreversible brain damage, including cognitive impairment, increased dementia risk, debilitating depression, and other neuropsychiatric disorders. Current radioprotective drugs face limitations, such as single-target inefficacy or manufacturing hurdles. Isoliquiritigenin (ISL), a natural flavonoid derived from licorice root, exhibits broad bioactivities. It exhibits anti-inflammatory, anti-cancer, immunoregulatory, hepatoprotective, and cardioprotective activities. This study aimed to elucidate ISL’s neuronal radiation mitigation effects and key targets. Methods: In vitro and in vivo models of radiation-induced neuronal injury were established. ISL’s bioactivities were evaluated through cellular cytotoxicity assays, LDH release, ROS, ATP, glutamate, and GSH levels. In vivo, ISL’s radiation mitigation effect was evaluated with sucrose preference test, IL-β level, histopathological analysis, and Golgi-Cox staining analysis. Proteomics, pathway enrichment, and ensemble models (four machine learning models, weighted gene co-expression network, protein–protein interaction) identified core targets. Molecular docking and dynamic simulations validated ISL’s binding stability with key targets. Results: ISL attenuated radiation-induced cellular cytotoxicity, reduced LDH/ROS, restored ATP, elevated GSH, and mitigated glutamate accumulation. In rats, ISL alleviated anhedonia-like phenotypes and hippocampal synaptic loss. ISL also significantly suppressed radiation-induced neuroinflammation, as evidenced by reduced levels of the pro-inflammatory cytokine IL-1β. Proteomic analysis revealed that ISL’s main protective pathways included the synaptic vesicle cycle, glutamatergic synapse, MAPK signaling pathway, SNARE interactions in vesicular transport, insulin signaling pathway, and insulin secretion. Grm8, Grik3, and Grin3a were identified as key targets using the integrated models. The expression of these targets was upregulated post-radiation and restored by ISL. Molecular docking and dynamic simulations indicated that ISL showed stable binding to these receptors compared to native ligands. Conclusions: ISL demonstrates multi-scale radiation mitigation activities in vitro and in vivo by modulating synaptic and inflammatory pathways, with glutamate receptors as core targets. This work nominates ISL as an important natural product for mitigating radiotherapy-induced neural damage. Full article

Recycled water is commonly considered an environmentally friendly alternative water source for urban water systems, which can not only serve as a solution for water scarcity, but also reduce wastewater discharge from sewage systems. However, owing to the high degree of energy consumption during recycled water production, the utilization of recycled water may be detrimental to greenhouse gas emission reduction. In this work, we conduct a detailed investigation into greenhouse gas emissions from different sources in a typical multisource urban water system in China. Furthermore, we develop an optimization model for water resource allocation based on the rime optimization algorithm and regret theory. The results show that although greenhouse gas emissions from recycled water exceed those from other sources, their impact can be eliminated through rational water resource allocation. Specifically, compared with the original water resource allocation, the optimal results effectively reduce pollutant emissions by 7.6~11.1% without excessively increasing water resource shortages and greenhouse gas emissions. Additionally, both subjective preferences and recycled water utilization conditions have significant impacts on the optimization results, which should be carefully selected according to practical situations and technologies. Overall, the methods developed in this study provide a new general framework for the water resource allocation of multisource urban water systems in the context of greenhouse gas emission reduction and recycled water utilization, which can be employed in other areas. Full article

Understanding long-term movement ecology is critical for conserving endangered species; however, comprehensive spatiotemporal analyses remain limited. In this study, we leveraged a decade-long GPS tracking dataset (2014–2024) of 31 endangered Crested Ibis (Nipponia nippon) individuals to elucidate their spatiotemporal behavioral patterns. The study focused on three key aspects: (1) fidelity to nesting, foraging, and roosting sites; (2) movement patterns and their ecological drivers; and (3) foraging habitat preferences across regions and activity periods. The results revealed exceptional fidelity to nesting, foraging (mean value = 0.253), and roosting sites (mean value = 0.261), underscoring the species’ pronounced spatial memory. Temporal factors emerged as the primary drivers of movement patterns, demonstrated by a significant annual reduction in home range size (p < 0.01) and a decline in daily flight distance in 2019 (β = −1890 ± 772 m, p < 0.05) and 2022 (p = 0.052). Behavioral factors also significantly influenced daily flight distance, with notable variations across different activity periods. Foraging habitat selection exhibited considerable spatial heterogeneity (14.2% constrained variance, p < 0.01). Cultivated lands, particularly paddy fields (Yangxian population) and drylands (Tongchuan population), served as core foraging zones. In contrast, spatiotemporal variables such as age had limited effects (<5% variance). This study provides the first empirical evidence of long-term site fidelity and habitat partitioning in the Crested Ibis, emphasizing the importance of landscape-level conservation planning. To this end, we propose two targeted strategies: establishing habitat corridors to enhance connectivity and safeguarding stable foraging areas within agricultural landscapes. These findings contribute to movement ecology theory while offering actionable frameworks for endangered species management. Full article

The complex topography of China’s Tibetan Plateau mountainous roads, characterized by diverse curve types and frequent traffic accidents, significantly impacts the safety and sustainability of the transportation system. To enhance driving safety on these mountain roads and promote low-carbon, resilient transportation development, this study investigates the mechanisms through which different curve types affect driving safety and proposes optimization strategies based on interpretable machine learning methods. Focusing on three typical curve types in plateau regions, drone high-altitude photography was employed to capture footage of three specific curves along China’s National Highway G318. Oblique photography was utilized to acquire road environment information, from which 11 data indicators were extracted. Subsequently, 8 indicators, including cornering preference and vehicle type, were designated as explanatory variables, the curve type indicator was set as the dependent variable, and the remaining indicators were established as safety assessment indicators. Linear models (logistic regression, ridge regression) and non-linear models (Random Forest, LightGBM, XGBoost) were used to conduct model comparison and factor analysis. Ultimately, three non-linear models were selected, employing an explainability-oriented dynamic ensemble learning strategy (X-DEL) to evaluate the three curve types. The results indicate that non-linear models outperform linear models in terms of accuracy and scene adaptability. The explainability-oriented dynamic ensemble learning strategy (X-DEL) is beneficial for the construction of driving safety models and factor analysis on Tibetan Plateau mountainous roads. Furthermore, the contribution of indicators to driving safety varies across different curve types. This research not only deepens the scientific understanding of safety issues on plateau mountainous roads but, more importantly, its proposed solutions directly contribute to building safer, more efficient, and environmentally friendly transportation systems, thereby providing crucial impetus for sustainable transportation and high-quality regional development in the Tibetan Plateau. Full article

This study evaluated the effects of adding a Saccharomyces cerevisiae fermentation product (SCFP) to adult cat diets on palatability, intestinal health, nutrient digestibility, immune parameters, and the fecal microbiome over 42 days. Sixty-three healthy Domestic Short-hair cats were randomized to three diets: a control diet (CD) without SCFP, or the same diet containing 1.0% or 2.0% SCFP, targeting daily intakes of 150 or 300 mg/kg body weight, respectively. Body weight and blood parameters remained within reference ranges across all groups. Stool quality was largely not affected, although compared with controls, SCFP-supplemented cats had slightly firmer stools at day 21, and increased fecal pH. Shotgun metagenomic sequencing revealed that microbiome diversity remained steady in SCFP-fed cats, whereas diversity in the control group declined over time. Fecal immunoglobulin A concentrations were lower in cats supplemented with SCFP at 150 mg/kg BW by the end of the study, and positive shifts in the circulatory leukocyte profile were observed at both inclusion levels. Apparent total tract macronutrient digestibility did not differ among groups. Palatability tests showed diets with SCFP were generally preferred, indicating a potential benefit for commercial feline feeds, particularly at the 150 mg/kg BW level, which was preferred over 300 mg/kg BW. Overall, these findings suggest that SCFP can act as a functional ingredient in feline nutrition to maintain microbial diversity and enhance diet acceptance without compromising digestibility. Full article

To investigate granulocytes under laboratory conditions, centrifugation steps are typically required for the isolation of neutrophil granulocytes from whole blood. However, only a few studies to date have addressed the direct effects of centrifugation itself on the functional state of neutrophils. This study aims to elucidate the mechanisms that contribute to the modification of granulocytes during centrifugation. We hypothesize that granules sustain morphological alterations during centrifugation, leading to the release of highly potent antimicrobial enzymes into the cytosol of the cells. Neutrophils were isolated from whole blood using different methods with and without centrifugation and analyzed by flow cytometry, ELISA, and mass spectrometry. Our findings demonstrate that intracellular granules incur damage during centrifugation, resulting in the presence of intragranular enzymes within the cytosol. Furthermore, the formation of the highly reactive hypochlorous acid (HOCl) as a consequence of centrifugation could be verified. The generation of intracellular HOCl may explain many of the alterations observed in neutrophils following centrifugation-based isolation, including modified surface antigen expression and altered responses to stimulation. In future studies, centrifugation steps during cell isolation should be avoided. The more time-consuming but gentler method of sedimentation is preferable and can be used as long as it is not necessary to obtain a highly purified neutrophil fraction. Full article

Zenker’s diverticulum (ZD) is an esophageal condition that results in an outpouching of the mucosal layer through a weakened area in the hypopharyngeal wall. This condition can cause symptoms like dysphagia, regurgitation, and aspiration, impacting patients’ quality of life. Historically, open surgery was the primary treatment. Although effective, this method is associated with longer recovery times and risks such as infections, nerve damage, and prolonged hospitalization. Rigid endoscopic stapling emerged as a less invasive alternative, offering high success rates for patients with favorable anatomy. Zenker’s peroral endoscopic myotomy (Z-POEM), adapted from treatments for achalasia, represents the latest advancement in ZD management. It involves creating a submucosal tunnel and precisely dividing the cricopharyngeus muscle. Z-POEM is minimally invasive and often provides quick relief with a high success rate of around 92%, while enabling outpatient treatment or brief hospital stays. However, it requires specialized expertise, and long-term data on recurrence rates are still emerging. This review discusses the evolution of these treatment modalities through comprehensive searches of PubMed, MEDLINE, and ScienceDirect databases. Studies reporting on treatment outcomes, complication rates, operative times, and clinical success associated with open surgery, rigid endoscopic stapling, and Z-POEM were included, with emphasis on meta-analyses, multicenter studies, and large case series highlighting Z-POEM’s comparable success to open surgery and increased patient tolerance. Open surgery achieves long-term symptom resolution rates of 90–95% but is associated with higher complication rates (up to 30%) and prolonged recovery times. Rigid endoscopic stapling offers symptom relief in approximately 90% of cases, with lower morbidity and shorter hospital stays (1–2 days), though anatomical limitations restrict its use. Z-POEM has demonstrated clinical success rates of 85.5–93%, with major complications reported in 4.8–5% of cases and recurrence rates as low as 1.4% at one-year follow-up in larger diverticula. Z-POEM’s minimally invasive nature and suitability for high-risk patients make it increasingly preferred in specialized centers. Management of Zenker’s diverticulum has evolved significantly, with endoscopic techniques, particularly Z-POEM, offering comparable success to open surgery but with fewer complications and faster recovery. Ongoing advances in endoscopic equipment and technique, along with emerging data on long-term outcomes, are likely to further refine treatment algorithms for ZD, especially for elderly and high-risk populations. Future directions in ZD management include ongoing research to enhance the safety and efficacy of endoscopic techniques, with new technologies on the horizon that could further improve outcomes and accessibility. Full article