Search Results (25)

The large-scale generation of industrial waste salts (IWSs) across sectors such as coal chemical, pesticide, pharmaceutical, and dye manufacturing has raised increasing environmental and regulatory concerns. These IWSs often exhibit complex physicochemical profiles—featuring high concentrations of inorganic salts, persistent organic pollutants, and trace heavy metals—that pose significant challenges for both safe disposal and resource recovery. This review provides a comprehensive and pollutant-oriented overview of industrial waste salts, focusing on their sector-specific characteristics, dominant contaminant types, and tailored treatment strategies. Removal pathways for organic matter (e.g., thermal decomposition, advanced oxidation) and inorganic impurities (e.g., precipitation, ion exchange) are systematically analyzed, followed by technical pathways for salt separation based on crystallization and membrane processes. Resource utilization routes for major salt components, particularly NaCl and Na₂SO₄, are critically assessed in terms of technical feasibility, impurity tolerance, and end-use compatibility. The emergence of reclaimed salt quality standards and sector-specific impurity thresholds reflects a paradigm shift from purity-based to performance-based reuse evaluation. Finally, the review highlights future priorities including adaptive impurity control, downstream-specific salt grading, and enforceable regulatory frameworks to ensure the safe, scalable, and circular deployment of reclaimed salts in industrial systems. This study supports the coordinated advancement of control technologies and reuse standards, enabling the transformation of waste salts from environmental liabilities to secondary resources. Full article

Lactoferrin (Lf) occurs predominantly within milk, coexisting with measurable levels across different glandular products and body fluids. Lf exhibits variation in relative molecular mass, influenced by its biological source and glycosylation profile; nevertheless, it is a close to 80 kDa glycoprotein. Provided that its bioactive structure is preserved, Lf performs a spectrum of physiological roles, comprising antioxidant, antifungal, antiviral, antiapoptotic, and antimicrobial actions. To sustain its bioactivity after isolation and ensure its effectiveness in subsequent applications, optimal conditions must be established throughout the optimization protocol, since inadequate optimization of parameters such as pH, temperature, ion balance, and protease activity may lead to aggregation, denaturation, and deterioration in functional regions, including the iron-binding domains. This review offers a comprehensive framework that associates isolation methodologies with structural integrity, preservation of iron-binding domains, and antimicrobial performance. Ion-exchange, affinity-based, and membrane-based approaches are systematically evaluated from analytical and functional perspectives, thereby yielding a synthesis that facilitates procedure selection and optimization for Lf isolation. In addition, the objectives of analytical characterization techniques implemented following isolation and the broadening scope of biotechnological applications of Lf are outlined. Full article

Background: Borrelia burgdorferi, the causative agent of Lyme disease, releases outer membrane vesicles (OMVs) that may contribute to infection and modulate the host immune response. Although interest in OMVs is growing, few studies have systematically compared methods for isolating OMVs from B. burgdorferi. Methods: In this study, we evaluated two OMV isolation techniques—standard ultracentrifugation and an ion-exchange chromatography-based ExoBacteria™ kit—and examined how serum supplements (rabbit serum vs. exosome-depleted fetal bovine serum, ED-FBS) influence Bb-OMV yield and composition. Gene expression profiles were assessed using RT-PCR, and specific protein content was identified by Western blot analyses. To assess the ability of Bb-OMVs to interact with host cells, Bb-OMVs were co-cultured with MDA-MB-231 triple-negative breast cancer cells. Results: Transmission electron microscopy confirmed that both methods produced spherical Bb-OMVs with intact membrane bilayers. Ultracentrifugation generated larger vesicles (15–180 nm), while the ExoBacteria™ kit yielded smaller vesicles (<50 nm) with a higher double-stranded DNA (dsDNA) content, and protein levels were similar across samples. Cultures grown with rabbit serum produced more Bb-OMVs and had cleaner backgrounds in the TEM images than those grown with ED-FBS. All Bb-OMV samples lacked intracellular markers (DnaK and 16S rRNA) and consistently expressed the outer surface protein OspA, confirming high purity. All isolated Bb-OMVs were taken up by the cells, as indicated by OspA expression, without detectable 16S rRNA, confirming vesicle internalization without bacterial contamination. Conclusions: These findings indicate that isolated OMVs are biologically active and capable of interacting with mammalian cells, highlighting their potential role in host–pathogen interactions and the broader relevance of OMVs in studying bacterial modulation of mammalian cell behavior. Overall, both isolation methods produced high-quality OMVs, with ultracentrifugation yielding slightly more pure vesicles, emphasizing the importance of selecting appropriate isolation methods and culture conditions for functional OMV studies. Full article

This study evaluates the feasibility of employing rainwater as an alternative feedstock for hydrogen production via electrolysis. While conventional systems typically rely on high-purity water—such as deionized or distilled variants—these can be cost-prohibitive and environmentally intensive. Rainwater, being naturally available and minimally treated, presents a potential sustainable alternative. In this work, a series of comparative experiments was conducted using a proton exchange membrane electrolyzer system operating with both deionized water and rainwater collected from different Austrian locations. The chemical composition of rainwater samples was assessed through inductively coupled plasma, ion chromatography and visual rapid tests to identify impurities and ionic profiles. The electrolyzer’s performance was evaluated under equivalent operating conditions. Results indicate that rainwater, in some cases, yielded comparable or marginally superior efficiency compared to deionized water, attributed to its inherent ionic content. The study also examines the operational risks linked to trace contaminants and explores possible strategies for their mitigation. Full article

This study investigates the behavior of phosphate ion transport through two structurally distinct anion-exchange membranes—AMV (homogeneous) and HC-A (heterogeneous)—in an electrodialysis system under both static and stirred conditions at varying pH levels. Chronopotentiometric and current–voltage analyses were used to investigate the influence of pH and hydrodynamics on ion transport. Under underlimiting (ohmic) conditions, the AMV membrane exhibited simultaneous transport of H₂PO₄⁻ and HPO₄²⁻ ions at neutral and mildly alkaline pH, while such behavior was not verified at acidic pH and in all cases for the HC-A membrane. Under overlimiting current conditions, AMV favored electroconvection at low pH and exhibited significant water dissociation at high pH, leading to local pH shifts and chemical equilibrium displacement at the membrane–solution interface. In contrast, the HC-A membrane operated predominantly under strong electroconvective regimes, regardless of the pH value, without evidence of water dissociation or equilibrium change phenomena. Stirring significantly impacted the electrochemical responses: it altered the chronopotentiogram profiles through the emergence of intense oscillations in membrane potential drop at overlimiting currents and modified the current–voltage behavior by increasing the limiting current density, reducing electrical resistance, and compressing the plateau region that separates ohmic and overlimiting regimes. Additionally, both membranes showed signs of NH₃ formation at the anodic-side interface under pH 7–8, associated with increased electrical resistance. These findings reveal distinct ionic transport characteristics and hydrodynamic sensitivities of the membranes, thus providing valuable insights for optimizing phosphate recovery via electrodialysis. Full article

A potentiometric electronic tongue (ET) for the analysis of well and ditch irrigation water samples is herein proposed. The sensors’ array is composed of six ion-selective electrodes based on plasticized polymeric membranes with low selectivity profiles, i.e., the membranes do not contain any selective receptor. The sensors differ between them in the type of ion-exchanger (sensors for cations or anions) and the plasticizer used in the membrane composition, while the polymeric matrix and the preparation protocol were maintained. The potentiometric response of each sensor towards the main cations (Na⁺, K⁺, Ca²⁺, Mg²⁺) and anions (HCO₃⁻, Cl⁻, SO₄²⁻, NO₃⁻) expected in irrigation water samples was characterized, revealing a fast response time (<50 s). A total of 19 samples were analyzed with the sensor array at optimized experimental conditions, but, also, a series of complementary analytical techniques were applied to obtain the exact ion composition and conductivity to develop a trustable ET. The principal component analysis of the final potential values of the dynamic response observed with each sensor in the array allows for the differentiation between most of the samples in terms of quality. Furthermore, the ET was treated with a linear multivariate regression method for the quantitative determination of the mentioned ions in the irrigation water samples, revealing rather good prediction of Mg²⁺, Na⁺, and Cl⁻ concentrations and acceptable results for the rest of ions. Overall, the ET is a promising analytical tool for irrigation water quality, exceeding traditional characterization approaches (conductivity, salinity, pH, cations, anions, etc.) in terms of overhead costs, versatility, simplicity, and total time for data provision. Full article

Membrane transporters and ion channels that play an indispensable role in metabolite trafficking have evolved to operate in Earth’s gravity. Dysregulation of the transportome expression profile at normogravity not only affects homeostasis along with drug uptake and distribution but also plays a key role in the pathogenesis of diverse localized to systemic diseases including cancer. The profound physiological and biochemical perturbations experienced by astronauts during space expeditions are well-documented. However, there is a paucity of information on the effect of the space environment on the transportome profile at an organ level. Thus, the goal of this study was to analyze the effect of spaceflight on ion channels and membrane substrate transporter genes in the periparturient rat mammary gland. Comparative gene expression analysis revealed an upregulation (p < 0.01) of amino acid, Ca²⁺, K⁺, Na⁺, Zn²⁺, Cl⁻, PO₄³⁻, glucose, citrate, pyruvate, succinate, cholesterol, and water transporter genes in rats exposed to spaceflight. Genes associated with the trafficking of proton-coupled amino acids, Mg²⁺, Fe²⁺, voltage-gated K⁺-Na⁺, cation-coupled chloride, as well as Na⁺/Ca²⁺ and ATP-Mg/Pi exchangers were suppressed (p < 0.01) in these spaceflight-exposed rats. These findings suggest that an altered transportome profile contributes to the metabolic modulations observed in the rats exposed to the space environment. Full article

For a theoretical analysis of mass transfer processes in electromembrane systems, the Nernst–Planck and Poisson equations (NPP) are generally used. In the case of 1D direct-current-mode modelling, a fixed potential (for example, zero) is set on one of the boundaries of the considered region, and on the other—a condition connecting the spatial derivative of the potential and the given current density. Therefore, in the approach based on the system of NPP equations, the accuracy of the solution is significantly affected by the accuracy of calculating the concentration and potential fields at this boundary. This article proposes a new approach to the description of the direct current mode in electromembrane systems, which does not require boundary conditions on the derivative of the potential. The essence of the approach is to replace the Poisson equation in the NPP system with the equation for the displacement current (NPD). Based on the system of NPD equations, the concentration profiles and the electric field were calculated in the depleted diffusion layer near the ion-exchange membrane, as well as in the cross section of the desalination channel under the direct current passage. The NPD system, as well as NPP, allows one to describe the formation of an extended space charge region near the surface of the ion-exchange membrane, which is important for describing overlimiting current modes. Comparison of the direct-current-mode modelling approaches based on NPP and NPD showed that the calculation time is less for the NPP approach, but the calculation accuracy is higher for the NPD approach. Full article

During the COVID-19 pandemic, wastewater-based epidemiology (WBE) has proven to be an effective tool for monitoring the prevalence of SARS-CoV-2 in urban communities. However, low-cost, simple, and reliable wastewater sampling techniques are still needed to promote the widespread adoption of WBE in many countries. Since their first use for public health surveillance in the 1950s, many types of passive samplers have been proposed, however, there have been few systematic studies comparing their ability to co-capture enveloped viruses and bacteria. Here, we evaluated the laboratory and field performance of 8 passive sampler materials (NanoCeram, ZetaPlus, nylon and ion exchange membranes, cellulose acetate filters, glass wool, cotton-based Moore swabs and tampons) to capture viruses and bacteria from wastewater. Viral capture focused on SARS-CoV-2, the bacteriophage Phi6 and the faecal marker virus, crAssphage. We showed that the best performing passive sampler in terms of cost, ease of deployment and viral capture were the electronegative cotton-based swabs and tampons. We speculate that viral capture is a combination of trapping of particulate matter to which viruses are attached, as well as electrostatic attraction of viral particles from solution. When deployed at wastewater treatment plants, the passive samplers worked best up to 6 h, after which they became saturated or exhibited a loss of virus, probably due to night-time wash-out. The patterns of viral capture across the different sampling materials were similar providing evidence that they can be used to monitor multiple public health targets. The types of bacteria trapped by the passive samplers were material-specific, but possessed a different 16S rRNA gene profile to the wastewater, suggesting preferential retention of specific bacteria. We conclude that the choice of passive sampler and deployment time greatly influences the pattern and amount of viral and bacterial capture. Full article

Profiled ion-exchange membranes are promising for improving the parameters of reverse electrodialysis due to the reduction of pumping power and electrical resistance. The smooth commercial heterogeneous cation-exchange MK-40 and anion-exchange MA-41 membranes were chosen as the initial membranes. Profiled membranes with three different types of surface profiles were obtained by hot pressing the initial membranes. The bilayer membranes were made on the basis of single-layer profiled membranes by casting MF-4SK film on the profiled surfaces. The diffusion permeability of all types of single-layer and bilayer profiled membranes was higher than of the initial ones due to the appearance of large defects on their surface during pressing. The conductivity of the profiled membrane was lower in the diluted solution and higher in the concentrated solution than of the initial one for all samples except for the bilayer anion-exchange membrane. The conductivity of that sample was lower than that of the initial anion-exchange MA-41 membrane over the entire range of studied concentrations. The counter-ion transport numbers for all studied membranes were calculated based on the concentration dependences of conductivity and diffusion permeability of the membrane by the microheterogeneous model. The selectivity of single layer and bilayer profiled membranes became lower after their profiling due to the increase of the solution phases of membranes. The asymmetry of the current-voltage curves for all single-layer and bilayer profiled membranes was found. The application of the single layer and bilayer profiled membranes in reverse electrodialysis did not lead to an increase in power density. Full article

Microbial electrosynthesis (MES) can sustainably convert CO₂ to products and significant research is currently being conducted towards this end, mainly in laboratory-scale studies. The high-cost ion exchange membrane, however, is one of the main reasons hindering the industrialization of MES. This study investigates the conversion of CO₂ (as a sole external carbon source) to CH₄ using membraneless MES inoculated with anaerobic granular sludge. Three types of electrodes were tested: carbon cloth (CC) and CC functionalized with Cu NPs, where Cu NPs were deposited for 15 and 45 min, respectively. During the MES experiment, which lasted for 144 days (six cycles), methane was consistently higher in the serum bottles with CC electrodes and applied voltage. The highest CH₄ (around 46%) was found in the second cycle after 16 days. The system’s performance declined during the following cycles; nevertheless, the CH₄ composition was twice as high compared to the serum bottles without voltage. The MES with Cu NPs functionalized CC electrodes had a higher performance than the MES with plain CC electrodes. Microbial profile analysis showed that the Methanobacterium was the most dominant genus in all samples and it was found in higher abundance on the cathodes, followed by the anodes, and then in the suspended biomass. The genus Geobacter was identified only on the anodes regarding relative bacterial abundance at around 6–10%. Desulfovibrio was the most dominant genus in the cathodes; however, its relative abundance was significantly higher for the cathodes with Cu NPs. Full article

The harmful impacts of fossil-fuel-based engines on the environment have resulted in the development of other alternatives for different types of vehicles. Currently, batteries and fuel cells are being used in the automotive industry, while promising progress in the maritime and aerospace sectors is foreseen. As a case study in the aerospace sector, an unmanned aerial vehicle (UAV) was considered. The goal and the novelty of this study are in its analysis of the possibility of providing 960 W of power for a UAV with a weight of 14 kg using a hybrid system of a lithium-ion (Li-ion) battery and proton-exchange membrane fuel cell (PEMFC). The dynamic performance of the system was analyzed considering three different load profiles over time in an optimized condition. PEMFC was the main supplier of power, while the battery intervened when the power load was high for the PEMFC and the system demanded an immediate response to the changes in power load. Additionally, the impacts of the operating temperature and the C-rate of the battery were characterized by the state of the charge of the battery to better indicate the overall performance of the system. Full article

Canavalia rosea, distributed in the coastal areas of tropical and subtropical regions, is an extremophile halophyte with good adaptability to high salinity/alkaline and drought tolerance. Plant sodium/hydrogen (Na⁺/H⁺) exchanger (NHX) genes encode membrane transporters involved in sodium ion (Na⁺), potassium ion (K⁺), and lithium ion (Li⁺) transport and pH homeostasis, thereby playing key roles in salinity tolerance. However, the NHX family has not been reported in this leguminous halophyte. In the present study, a genome-wide comprehensive analysis was conducted and finally eight CrNHXs were identified in C. rosea genome. Based on the bioinformatics analysis about the chromosomal location, protein domain, motif organization, and phylogenetic relationships of CrNHXs and their coding proteins, as well as the comparison with plant NHXs from other species, the CrNHXs were grouped into three major subfamilies (Vac-, Endo-, and PM-NHX). Promoter analyses of cis-regulatory elements indicated that the expression of different CrNHXs was affected by a series of stress challenges. Six CrNHXs showed high expression levels in five tested tissues of C. rosea in different levels, while CrNHX1 and CrNHX3 were expressed at extremely low levels, indicating that CrNHXs might be involved in regulating the development of C. rosea plant. The expression analysis based on RNA-seq showed that the transcripts of most CrNHXs were obviously decreased in mature leaves of C. rosea plant growing on tropical coral reefs, which suggested their involvement in this species’ adaptation to reefs and specialized islands habitats. Furthermore, in the single-factor stress treatments mimicking the extreme environments of tropical coral reefs, the RNA-seq data also implied CrNHXs holding possible gene-specific regulatory roles in the environmental adaptation. The qRT-PCR based expression profiling exhibited that CrNHXs responded to different stresses to varying degrees, which further confirmed the specificity of CrNHXs’ in responding to abiotic stresses. Moreover, the yeast functional complementation test proved that some CrNHXs could partially restore the salt tolerance of the salt-sensitive yeast mutant AXT3. This study provides comprehensive bio-information and primary functional identification of NHXs in C. rosea, which could help improve the salt/alkaline tolerance of genetically modified plants for further studies. This research also contributes to our understanding of the possible molecular mechanism whereby NHXs maintain the ion balance in the natural ecological adaptability of C. rosea to tropical coral islands and reefs. Full article

Nowadays, many aircraft manufacturers are working on new airplanes to reduce the environmental footprint and therefore meet greenhouse gas reduction targets. The concept of more electric aircraft is one of the solutions to achieve this goal. For this aircraft architecture, several electrical devices are used in order to supply propulsive and non-propulsive functions. This paper focuses on the sizing of a direct hybridization system to supply a non-propulsive function in an aircraft. It is composed of a High-Temperature Proton Exchange Membrane Fuel Cell (HT-PEMFC) and a lithium-ion (Li-ion) battery. This sizing is based on a static model of each storage device. The accuracy of these models is compared with dynamic models during a simulation for an aeronautical mission. Static models are implemented in a genetic algorithm to achieve two goals: on the one hand, satisfy the mission profile, and on the other hand, minimize the mass of the system. Other criteria, such as battery and fuel cell aging estimation, are considered. The obtained results show that the direct hybridization system allows protecting the fuel cell against an accelerated aging. Full article

Perfluorosulfonated ionomers are the most successful ion-exchange membranes at an industrial scale. One recent, cutting-edge application of perfluorosulfonated ionomers is in polymer electrolyte fuel cells (PEFCs). In PEFCs, the ionomers are used as a component of the catalyst layer (CL) in addition to functioning as a proton-exchange membrane. In this study, the microstructures in the CLs of PEFCs were characterized by combined synchrotron X-ray scattering and transmission electron microscopy (TEM) analyses. The CL comprised a catalyst, a support, and an ionomer. Fractal dimensional analysis of the combined ultrasmall- and small-angle X-ray scattering profiles indicated that the carbon-black-supported Pt catalyst (Pt/CB) surface was covered with the ionomer in the CL. Anomalous X-ray scattering revealed that the Pt catalyst nanoparticles on the carbon surfaces were aggregated in the CLs. These findings are consistent with the ionomer/catalyst microstructures and ionomer coverage on the Pt/CB surface obtained from TEM observations. Full article