Search Results (107)

Fungal mycelial pellets (MPs) exhibit high biomass-loading capacity; however, their application in wastewater treatment is constrained by structural fragility and the risk of environmental dispersion. To overcome these limitations, a dual-crosslinked polyvinyl alcohol–alginate gel (10% PVA, 2% sodium alginate) embedding strategy was developed and stabilized using 2% CaCl₂ and saturated boric acid. This encapsulation enhanced the tensile strength of MPs by 499% (310.4 vs. 62.1 kPa) and improved their settling velocity by 2.3-fold (1.12 vs. 0.49 cm/s), which was critical for stability under turbulent bioreactor conditions. Following encapsulation, the specific oxygen uptake rates (SOURs) of three fungal strains (F557, Y3, and F507) decreased by 30.3%, 54.8%, and 48.3%, respectively, while maintaining metabolic functionality. SEM revealed tight adhesion between the gel layer and both surface and internal hyphae, with the preservation of porous channels conducive to microbial colonization. In sequential-batch reactors treating sulfamethoxazole (SMX)-contaminated wastewater, gel-encapsulated MPs combined with acclimated sludge consistently achieved 72–75% SMX removal efficiency over six cycles, outperforming uncoated MPs (efficiency decreased from 81.2% to 58.7%) and pure gel–sludge composites (34–39%). The gel coating inhibited hyphal dispersion by over 90% and resisted mechanical disintegration under 24 h agitation. This approach offers a scalable and environmentally sustainable means of enhancing MPs’ operational stability in continuous-flow systems while mitigating fungal dissemination risks. Full article

Seasonal temperature variations significantly impact biological wastewater treatment performance, particularly affecting extracellular polymeric substance (EPS) composition and sludge settling characteristics in activated sludge systems. This study investigated the temperature-induced EPS response mechanisms and their effects on nitrogen removal efficiency in a full-scale modified Bardenpho wastewater treatment plant, combined with laboratory-scale evaluation of EPS-optimizing microbial agents for performance enhancement. Nine-month seasonal monitoring revealed that when the wastewater temperature dropped below 15 °C, the total nitrogen (TN) removal efficiency decreased from 86.5% to 80.6%, with a trend of significantly increasing polysaccharides (PS) in dissolved organic matter (DOM) and loosely-bound EPS (LB-EPS) and markedly decreasing tightly-bound EPS (TB-EPS). During the low-temperature periods, when the sludge volume index (SVI) exceeded 150 mL/g, deteriorated settling performance could primarily be attributed to the reduced TB-EPS content and increased LB-EPS accumulation. Microbial community analysis showed that EPS secretion-promoting genera of Trichococcus, Terrimonas, and Defluviimonas increased during the temperature recovery phase rather than initial temperature decline phase. Laboratory-scale experiments demonstrated that EPS-optimizing microbial agents dominated by Mesorhizobium (54.2%) effectively reduced protein (PN) and PS contents in LB-EPS by 70.2% and 54.5%, respectively, while maintaining stable nutrient removal efficiency. These findings provide mechanistic insights into temperature–EPS interactions and offer practical technology for improving winter operation of biological wastewater treatment systems. Full article

The biotechnological conversion of CO₂ to biomethane represents an energy-efficient, environmentally friendly, and sustainable approach within the waste-to-energy cycle. This process, in which CO₂ and H₂ are converted to biomethane in anaerobic bioreactors, is referred to as hydrogenotrophic biomethane production. While several studies have investigated hydrogenotrophic biomethane production, there is a lack of research comparing flocculent and granular sludge inoculum in continuously operated systems fed with a gas substrate. Both granular and flocculent sludge possess distinct advantages: granular sludge offers higher density, stronger microbial cohesion, and superior settling performance, whereas flocculent sludge provides faster substrate accessibility and more rapid initial microbial activity. In this study, two UASB (Upflow Anaerobic Sludge Blanket) reactors operated under mesophilic conditions were continuously fed with synthetic off-gas composed of pure H₂ and CO₂ in a 4:1 ratio and were compared in terms of microbial community shifts and their effects on hydrogenotrophic biomethane production. Biomethane production reached 75 ± 2% in the granular sludge reactor, significantly higher than the 64 ± 1.3% obtained with flocculent sludge. Although hydrogen consumption did not differ significantly, the granular sludge reactor exhibited higher CO₂ removal efficiency. Microbial analyses further revealed that granular sludge was more effective in supporting methanogenic archaea under conditions of gas substrate feeding. These findings offer advantageous suggestions for improving biogas production, enhancing waste gas management, and advancing sustainable energy generation. Full article

This study investigates an innovative hybrid treatment for compost-derived wastewater, combining aluminum-based electrocoagulation (EC), zeolite addition, and magnet assistance. Key experimental variables—presence/absence of magnet, stirring speed (250 and 350 rpm), and contact time (10–30 min)—were systematically varied to analyze process efficiency, electrode dissolution and mass loss, solid–liquid separation dynamics, and quantify energy input and Faraday efficiency (FE). Magnet-assisted processes achieved higher COD reduction at longer treatment times of 30 min and lower mixing speeds of 250 rpm, with up to 89.87%. The highest turbidity reduction of 98.59% is achieved after 20 min at 350 rpm. The magnetic field does not significantly affect the dissolution of Al electrodes, but over time, it helps reduce localized electrode damage, thereby supporting both process efficiency and electrode longevity. Magnetic fields improved sludge settling in shorter treatments by promoting faster aggregation. However, the energy input was generally higher with magnetic assistance. FE in the range of 50.89–65.82% indicates that the actual electrode loss is lower than theoretical. For the experiments conducted according to the L8 Taguchi experimental design, given the significance and contribution of factors to the process, the optimal combination is the absence of a magnet, 350 rpm, and 20 min. Full article

This study examines the effects of polyester and denim microfibers (MFs) on aerobic granular sludge (AGS) over a 42-day period. Treatment performance, granulation, and microbial community changes were assessed at 0, 10, 70, 210, and 1500 MFs/L. Reactors with 70 MFs/L achieved rapid granulation and showed improved settling by day 9, while 0 and 10 MFs/L reactors showed delayed granule formation, which was likely due to limited nucleation and weaker shear conditions. Severe clogging and frequent maintenance occurred at 1500 MFs/L. Despite > 98% MF removal in all reactors, treatment performance declined at higher MF loads. Nitrogen removal dropped from 93% to 68%. Phosphate removal slightly increased in reactors with no or low microfiber loads (96–99%), declined in reactors with 70 or 210 MFs/L (92–91%, 89–88%), and dropped significantly in the reactor with1500 MFs/L (86–70%, p < 0.05). COD removal declined with increasing MF load. Paracoccus (denitrifiers) dominated low-MF reactors; Acinetobacter (associated with complex organic degradation) and Nitrospira (nitrite-oxidizing genus) were enriched at 1500 MFs/L. Performance decline likely stemmed from nutrient transport blockage and toxic leachates, highlighting the potential threat of MFs to wastewater treatment and the need for upstream MF control. Full article

The aim of this study is to determine the characteristics of the chemical and mineral composition of sediment layers in a technogenic settling pond. This pond is located on urban land in Berezniki (Perm Krai, Russia), outside the territory of operating industrial facilities, and contains alkaline saline industrial wastes. The origin of this waste was related to sludge from the Solvay soda production process, which had been deposited in this pond over a long period of time. However, along with the soda waste, the pond also received wastewater from other industries. As a result, the accumulated sediment is characterized by variation in morphological properties both in depth and laterally. Five undisturbed columns were taken to study the composition of the accumulated sediment. The obtained samples were analyzed by X-ray diffraction (XRD), synchronous thermal analysis (STA), and X-ray fluorescence (XRF) analysis. The results showed that the mineral composition of bottom sediments in each layer of all studied columns is characterized by the predominance of calcite precipitated from wastewater. Along with calcite, due to the presence of magnesium and sodium in the solution, other carbonates precipitated—dolomite and soda (natron), as well as complex transitional carbonate phases (northupite and trona). Together with carbonate minerals, the chloride salts halite and sylvin, sulfate minerals gypsum and bassanite, and pyrite and nugget sulfur were established. The group of terrigenous mineral components is represented by quartz, feldspars, and aluminosilicates. The chemical composition of sediments in the upper part of the section generally corresponds to the mineral composition. In the lower sediment layers, the role of amorphous phase and non-mineral compounds increased, which was determined by the results of thermal analysis. The content of heavy metals and metalloids also increases in the middle and lower sediment layers. When categorized according to the Igeo value, an excessive degree of contamination (class 6) was observed in all investigated columns for copper content (Igeo 5.2–6.1). Chromium content corresponds to class 5 (Igeo 4.1–4.6), antimony to class 4 (Igeo 3.0–4.0), and lead, arsenic, and vanadium to classes 2 and 3 (moderately polluted and highly polluted). The data obtained on variations in the mineral and chemical composition of sediments represent the initial information for the selection of methods of accumulated waste management. Full article

Microcystin-LR (MCLR) is a toxin produced by harmful algal blooms that is emerging as a threat to drinking and recreational water systems worldwide. Nanofiltration (NF) is an effective technique for purifying contaminated water sources; however, membrane fouling caused by coexisting organic matter limits the practicality of the process. This research studies the use of an electrocoagulation (EC) pretreatment to limit fouling during the NF process. Water for this study was taken from Lake Fayetteville, a local body of water where MCLR concentrations have been recorded to be >15 µg/L. EC was performed using polyaluminum chloride as a background electrolyte at various operating conditions. EC-treated water was then further treated with NF to assess the impact of the EC pretreatment on NF fouling. It was found that the larger particle size of the sludge produced using aluminum electrodes at pH 7 had the best combination of settling ability and organic carbon removal (92%). This also led to the smallest flux decline during six-hour NF experiments of just 9%. These results highlight the potential of an EC pretreatment as an antifouling technique for the NF treatment of water contaminated with algal toxins. Full article

The sustainable restoration of river and lake ecosystems requires advanced wastewater treatment technologies to control nitrogen pollution, a key driver of aquatic degradation. This study explores the physiological responses of anammox granular sludge (AnGS) to varying nitrogen loading rates (NLRs), offering insights into microbial stability under environmental stress. AnGS samples with different particle sizes (<1.0 mm, 1–2 mm, >2 mm) were subjected to NLRs ranging from 0.9 to 3.6 gN/L/d. As the NLR increased, the NO₂⁻-N/NH₄⁺-N consumption ratio rose from 1.0 to 1.2, and the most active particle size shifted to 1–2 mm. Hydroxyapatite (HAP) crystals formed at higher NLRs, enhancing the settling and activity of 1–2 mm AnGS but inhibiting larger granules (>2 mm). Microbial analysis revealed that Candidatus Brocadia dominated at high NLRs (10.5%), outperforming Candidatus Kuenenia (2.47%). The enrichment of these key genera across granules indicates adaptive microbial migration under loading stress. These findings provide critical operational strategies for sustaining AnGS performance through particle size regulation, contributing to nitrogen control solutions vital for river and lake restoration efforts. Full article

Industrial activated sludge plants in many sectors, including the dairy industry, face sludge separation problems caused by sludge bulking. Aerobic granular sludge (AGS) could be a solution by forming well-settling granules. The key to successful granulation is the microbial selection of slow-growing glycogen-accumulating organisms (GAOs) by introducing an anaerobic feeding/reaction step. The objective of the current study was to investigate the impact of two slow feeding strategies to achieve granulation in existing sequencing batch reactors treating real dairy wastewater, by microbial selection only. The first strategy consisted of slow 90 min mixed feeding. The second strategy combined 45 min static and 45 min mixed feeding to build up a substrate gradient. The feeding strategies did not affect the effluent quality, but significantly impacted the sludge morphology, settling properties, and microbial community composition. Mixed feeding led to filamentous overgrowth by Thiothrix species, up to 45% abundance, and deteriorating settling, with sludge volume index (SVI) values up to 125 mL/g. In contrast, static feeding yielded densified sludge with SVI values below 45 mL/g and up to 35% GAO abundance. In conclusion, the results show successful granulation when using a simple static slow feeding mode, which could benefit the industrial application of AGS technology. Full article

Water scarcity is a global crisis and of particular concern in arid regions like Morocco. One creative solution is mining unusual water sources, such as landfill leachate. The presence of nitrogen in the sediment was studied as part of the use of sugar lime sludge in treating landfill leachate for irrigation purposes. A volume of 40 L of landfill leachate was treated with three different concentrations of sugar lime sludge (25%, 35%, and 50%). After homogenization and agitation of the mixture for 24 to 36 h, it was permitted to settle through the concrete decantate and supernatant. Nitrogen was efficiently decanted into the sediment during the composting process with green waste, enhancing the quality of the finished compost. The supernatants underwent physicochemical and microbiological analyses to ascertain their suitability for irrigation. The findings showed that the number of fecal streptococci was decreased by 99.13% at a 25% concentration of sugar lime sludge. The percentage of organic matter in the sediment rose from 10% to 40%, suggesting that the leachate had partially depolluted. The pH and electrical conductivity of the supernatants were within irrigation guidelines. The safety of diluted supernatants for plant germination was verified by phytotoxicity experiments conducted on maize seeds. The compost made from the decantate and green waste showed acceptable physical and chemical properties. Statistical analysis was conducted using JAMOVI software version 2.6.26. One-way ANOVA was used to assess the significance of treatment effects on microbiological and physicochemical parameters. The results confirmed statistically significant differences (p < 0.05) between the sludge concentrations, supporting the effectiveness of the treatment process. This study demonstrates how sugar lime sludge can be used to turn landfill leachate into a sustainable and safe irrigation water source, resolving environmental issues and promoting creative water management techniques. Full article

Wastewater treatment plants (WWTPs) generate significant emissions of gaseous substances, such as H₂S, NH₃, and VOCs, which cause discomfort and pose health risks to residents in surrounding areas. The objective of this study was to estimate pollutant concentrations under various scenarios through a mathematical modeling of the pollutant dispersion in the surrounding air using the AERMOD View software platform, version 11.2.0. In this study, four mathematical models with two different scenarios were conducted to illustrate the odor concentrations both on site and in nearby areas under the most unfavorable weather conditions. The “1st Highest Values” and “98th Percentile” metrics were used to represent the peak concentrations and to exclude the 2% of conditions with the worst-case dispersion, respectively. In the first scenario, under normal operating conditions with all treatment equipment functioning, the maximum on-site odor concentration was estimated at 36.8 ouE/m³ using the 1st highest value function, and it was 20.4 ouE/m³ using the 98th percentile function. The second scenario considered all emission sources, with the grease collection system of the de-sanding/grease separation Unit Line 1 and the sludge collection system of the primary settling decanter (Unit 4) out of service. In this case, the maximum on-site odor concentration reached 749 ouE/m³ over 98% of a one-year period and 956.5 ouE/m³ using the 1st highest value function. These findings underscore the necessity for ongoing monitoring, strict adherence to environmental regulations, and stakeholder engagement to improve mitigation techniques and foster community trust in environmental management. Regular inspections are essential to ensure that all equipment operates within normal parameters, supporting both regulatory compliance and improved operational efficiency, including the control of odor emissions. Full article

Copper-containing sludge and spent petrochemical catalyst (SPC) were investigated for recovering palladium (Pd) and silver (Ag). Increasing the mixing ratio of alumina-based SPC leads to reduced recovery rates at 1500 °C. Specifically, as the SPC mixing ratio increases from 10% to 30%, the recovery rate of Pd and Ag sharply decreases to 62.1% and 91.0%, respectively. This is attributed to an increase in the slag viscosity as well as to the higher sulfur content in the metal phase by decreasing the CaO/Al₂O₃ ratio of the slag. An increase in the slag viscosity causes a decrease in the metal recovery, as it lowers the settling velocity of metal droplets, resulting in imperfect metal separation, i.e., an increase in physical loss. Additionally, the presence of sulfur at the slag–metal interface was found to reduce interfacial tension, facilitating the entrapment of copper droplets within the slag. This further hindered phase separation and contributed to an increase in physical loss. This study highlights that physical loss is more serious in metal recovery rather than chemical loss, which is dependent on the thermochemical solubility of the target metals in the slag. The results emphasize the need for the precise control of slag properties to maximize the metal recovery processes in conjunction with a mitigation of CO₂ emissions. Full article

Microplastics have emerged as a global environmental concern due to their widespread presence and potential effects on ecosystems. Wastewater treatment plants (WWTPs) play a critical role in mitigating the release of microplastics into the environment. This study aimed to evaluate the abundance and distribution of microplastics in three municipal WWTPs exhibiting different sludge and water treatment technologies. Samples were collected at various stages of the treatment process, including influent, primary and biological sludge, treated water, thickened sludge and dehydrated sludge. Quantification analyses were performed and then coupled with operational data to assess pollution flow rates and the microplastic balance. An important removal rate (>97%) of microplastics along the water line was observed in all three WWTPs. The lower performance of 0.75 mm screening was observed regarding microplastic capture compared to conventional primary settling on the water line. No significant differences in the pollution flow rates between primary and biological sludge were detected. Whatever the thickening and dehydration technologies that were tested, the specific quantities of microplastics along the sludge treatment lines were steady, implying the comparable behaviour of microparticular pollution to total suspended solids. These results underscored the important role of the different concentration stages of sludge treatment lines in sequestrating microplastics within the sludge fraction. Full article

The majority of microplastics (MPs) removed from urban wastewater during conventional treatments end up in sewage sludge (around 95%). There are not many studies focused on the retention of MPs in secondary sewage sludge. This study explores the amount and kind of MPs retained in secondary sludge from a municipal wastewater treatment plant (WWTP) and the factors affecting the entrapment of MPs within the sludge flocs. The most abundant MPs in the sludges were fibres (61%), and the majority were within the size range of 250–500 μm. In addition, the effect of solid concentration on MP retention in settled sludge was analysed by carrying out a mixing–settling test. Without the addition of any chemicals, a maximum MP retention efficacy of 63% was obtained for a total suspended solids (TSS) concentration of 5.33 g/L. The effect of adding FeCl₃ and non-ionic polyacrylamide (PAM) was also investigated, and the percentage of retained MPs increased to values of 82% and 70%, respectively (with only 0.63 g TSS/L). This improvement occurred predominantly in the case of fibres. The results suggest the possibility of adding chemicals before the secondary settling stage as a means of reducing MP contamination in treated water. Full article

The enhancement of the treatment of municipal wastewater treatment plants is limited by poor sludge settling qualities, and the excessive discharge of nitrogen and phosphorus exacerbate water eutrophication. The goal of the current work was to remove phosphorus from fresh sewage-activated sludge by developing a new conditioning and flocculation mechanism that included a coagulant and cationic polyelectrolytes in a dual conditioning system. The coagulant (CaCl₂) and the high molecular weight polyacrylamide (CPAM-10) were chosen to be utilized singly or in pairs as cationic–coagulant combinations. The collected results showed that, in comparison to utilizing the coagulant (CaCl₂), conditioning with the high molecular weight polymer (CPAM-10) produced improved settling and less turbidity. Only sludge with a lower solid content (TSS) exhibited better settling when pure CaCl₂ was used for conditioning. CaCl₂ conditioning enhanced settling by just 3%, while CPAM-10 improved the sludge setting by 60% for higher sludge TSSs. According to the results, conditioning settings using a dual mixture including 20 mL CPAM-10 and 50 mL CaCl₂ improved settling by 80%. The amount of phosphorus in the supernatant was decreased by 15% and 9%, respectively, by using the coagulant (CaCl₂) and 50 mL/L polyacrylamide (CPAM-10). As a result, there was a significant amount of phosphorus in the resultant supernatant. This suggested that the polymer had a significant impact on sludge settling because of its high positive charge, but had less of an impact on attracting phosphorus metal. Despite the lower positive charge of CaCl₂, it has a dual action of settling and removing phosphorus. A considerable amount of phosphorus was removed from the sludge and leached to the supernatant during treatment. This treatment was coupled with less sludge settling. However, 90% phosphorus removal was achieved when mixed conditioning agents (20 mL CPAM-10 and 50 mL CaCl₂) were used. Furthermore, phosphorus was reduced by 33 and 39%, respectively, by adding 20 milliliters of CaCl₂ to 100 milliliters of the pre-conditioned supernatant with pure CPAM-10 and CaCl₂. Using the CPAM-10 agent for sludge conditioning has a major impact on settling, because of the high positive charge, and because when a small amount of Ca++ is added to the polymer solution for conditioning to attract fine sludge particles and accelerate their combination, this results in flocculation and rapid dewatering. This mechanism allows for more phosphorus to be released to the supernatant, which has not been reported previously to the best of our knowledge. Full article