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18 pages, 5592 KB  
Article
Influence of a Diversion Pier on the Hydraulic Characteristics of an Inverted Siphon in a Long-Distance Water Conveyance Channel
by Jian Wang, Jingyu Hu, Xiaoli Yang, Lifang Lou, Tong Mu, Dongsheng Wang and Tengfei Hu
Water 2025, 17(16), 2378; https://doi.org/10.3390/w17162378 - 11 Aug 2025
Viewed by 370
Abstract
Since large-flow water diversion began in the middle route of the South-to-North Water Diversion Project, inverted siphons have experienced varying degrees of local flow pattern disorder at their inlets and outlets, resulting in a significant decline in hydraulic performance. Taking the Kuhe inverted [...] Read more.
Since large-flow water diversion began in the middle route of the South-to-North Water Diversion Project, inverted siphons have experienced varying degrees of local flow pattern disorder at their inlets and outlets, resulting in a significant decline in hydraulic performance. Taking the Kuhe inverted siphon as a case study, a combination of numerical simulation and on-site testing was used to explore the causes of flow pattern disorder at the outlet of the inverted siphon. Meanwhile, based on the actual engineering situation, the influence of the flow pattern optimization measure of installing a 5D (five times the diameter of the pier) diversion pier at the outlet of the inverted siphon on its hydraulic characteristics was studied. Research findings indicated that before the implementation of flow pattern optimization measures, the Karman vortex street phenomenon was found to occur when water flowed through the piers; the interaction of the vortex streets behind each pier led to flow pattern disorder and affected the flow capacity. After implementation of the flow pattern optimization measures, the diversion piers had a significant inhibitory effect on the formation and development of the Karman vortex street behind the piers under the dispatching and design flow conditions. The flow velocities in each vertical layer were adjusted, with a significant improvement in the flow pattern. The hydraulic loss of the Kuhe inverted siphon was reduced by 11.5 mm, or approximately 7.8%. Under the dispatching flow condition, the water diversion flow of the Kuhe inverted siphon increased by approximately 4.11%. The water diversion capacity of the structure could be effectively enhanced by adding diversion piers to the tails of the piers. This method can be widely applied in similar open-channel long-distance water diversion projects. Full article
(This article belongs to the Section Hydraulics and Hydrodynamics)
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23 pages, 5185 KB  
Article
Comparative Analysis of the NorSand and HS Small Constitutive Models for Evaluating Static Liquefaction in a Silt Derived from Mine Tailings
by Matias Muñoz-Gaete, Ricardo Gallardo, Edison Atencio, Ricardo Moffat, Pablo F. Parra, Carlos Cacciuttolo and William Araujo
Appl. Sci. 2025, 15(15), 8726; https://doi.org/10.3390/app15158726 - 7 Aug 2025
Viewed by 861
Abstract
The representation and assessment of static liquefaction in mine tailings is a significant challenge due to the severe environmental and social damage it can cause. This phenomenon, known for its catastrophic nature, is triggered when the undrained shear strength is exceeded by a [...] Read more.
The representation and assessment of static liquefaction in mine tailings is a significant challenge due to the severe environmental and social damage it can cause. This phenomenon, known for its catastrophic nature, is triggered when the undrained shear strength is exceeded by a static loading stress. In this study, the constitutive models HSS and NS were evaluated to calibrate the experimental curves from an isotropically consolidated undrained (CIU) triaxial test on a low-plasticity silt derived from mine tailings. An axisymmetric model was developed in Plaxis 2D for calibration, followed by a sensitivity analysis of the parameters of both constitutive models, using the RMSE to validate their accuracy. The results indicate that the proposed methodology adequately simulates the experimental curves, achieving an RMSE of 8%. After calibration, a numerical model was implemented to evaluate the propagation of the PFS of a mine tailings storage facility using both models, in terms of excess pore pressures, shear strains, and p’-q diagrams at three control points. The results show that both models are capable of representing the PFS; however, the HSS model reproduces the experimental curves more accurately, establishing itself as an ideal tool for simulating undrained behavior and, consequently, the phenomenon of static liquefaction in mine tailings. Full article
(This article belongs to the Special Issue Mining Engineering: Present and Future Prospectives)
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37 pages, 2373 KB  
Article
A Quantile Spillover-Driven Markov Switching Model for Volatility Forecasting: Evidence from the Cryptocurrency Market
by Fangfang Zhu, Sicheng Fu and Xiangdong Liu
Mathematics 2025, 13(15), 2382; https://doi.org/10.3390/math13152382 - 24 Jul 2025
Viewed by 1110
Abstract
This paper develops a novel modeling framework that integrates time-varying quantile-based spillover effects into a regime-switching realized volatility model. A dynamic spillover factor is constructed by identifying the most influential contributors to Bitcoin’s realized volatility across different quantile levels. This quantile-layered structure enables [...] Read more.
This paper develops a novel modeling framework that integrates time-varying quantile-based spillover effects into a regime-switching realized volatility model. A dynamic spillover factor is constructed by identifying the most influential contributors to Bitcoin’s realized volatility across different quantile levels. This quantile-layered structure enables the model to capture heterogeneous spillover paths under varying market conditions at a macro level while also enhancing the sensitivity of volatility regime identification via its incorporation into a time-varying transition probability (TVTP) Markov-switching mechanism at a micro level. Empirical results based on the cryptocurrency market demonstrate the superior forecasting performance of the proposed TVTP-MS-HAR model relative to standard benchmark models. The model exhibits strong capability in identifying state-dependent spillovers and capturing nonlinear market dynamics. The findings further reveal an asymmetric dual-tail amplification and time-varying interconnectedness in the spillover effects, along with a pronounced asymmetry between market capitalization and systemic importance. Compared to decomposition-based approaches, the X-RV type of models—especially when combined with the proposed quantile-driven factor—offers improved robustness and predictive accuracy in the presence of extreme market behavior. This paper offers a coherent approach that bridges phenomenon identification, source localization, and predictive mechanism construction, contributing to both the academic understanding and practical risk assessment of cryptocurrency markets. Full article
(This article belongs to the Section E5: Financial Mathematics)
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12 pages, 2674 KB  
Article
Effect of Lower-Level Relaxation on the Pulse Generation Performance of Q-Switched Nd:YAG Laser
by Fuqiang Ma, Shiyu Wang, Bingbin Li, Peijin Shang, Jinyou Li and Zheyuan Li
Photonics 2025, 12(5), 408; https://doi.org/10.3390/photonics12050408 - 24 Apr 2025
Cited by 1 | Viewed by 660
Abstract
When analyzing and designing Q-switched Nd:YAG lasers, the impact of lower-energy-level relaxation on the pulse waveform is often ignored. This approximation typically does not result in significant deviations when the laser pulse duration is much longer than the relaxation time of the lower [...] Read more.
When analyzing and designing Q-switched Nd:YAG lasers, the impact of lower-energy-level relaxation on the pulse waveform is often ignored. This approximation typically does not result in significant deviations when the laser pulse duration is much longer than the relaxation time of the lower energy level. However, when the pulse duration approaches the nanosecond range, the spontaneous emission time of lower energy level in the Nd:YAG crystal, which is approximately 30 ns, can severely affect the pulse waveform. In this study, a theoretical model is proposed to investigate the influence of lower-energy-level relaxation on the output pulse waveform of an Nd:YAG laser. Specifically, the output waveform of a narrow-pulse-width Q-switched Nd:YAG laser is simulated. The results indicate that for narrow-pulse-width laser output, lower-energy-level relaxation causes a secondary peak to appear after the main peak of the Q-switched pulse. The energy of this secondary peak is more than two times higher than that of the main peak. An experimental system for acousto-optic Q-switched Nd:YAG lasers has also been established, and the Q-switched pulse waveforms are measured under conditions similar to those in the simulations. The tail peak phenomenon observed in the experiments is consistent with the simulation results, verifying the accuracy of the theoretical model. These findings provide a crucial theoretical foundation for understanding and optimizing Nd:YAG lasers and have significant implications for the development of similar technologies. In laser technology, particularly for applications requiring high precision and performance, considering such factors is essential for optimizing the design and functionality of laser systems. Full article
(This article belongs to the Special Issue Photodetectors for Next-Generation Imaging and Sensing Systems)
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16 pages, 2378 KB  
Article
Ontogenesis from Embryo to Juvenile in Threadsail Filefish, Stephanolepis cirrhifer
by Liming Liu, Xuanhan Liu, Yanqing Wu, Jun Zeng and Wengang Xu
Animals 2025, 15(8), 1124; https://doi.org/10.3390/ani15081124 - 13 Apr 2025
Viewed by 788
Abstract
The threadsail filefish, Stephanolepis cirrhifer, is an economically important marine species. However, wild catches have sharply decreased over the past 20 years, causing S. cirrhifer to be added to the IUCN Red List of Threatened Species. Accordingly, this study seeks to promote [...] Read more.
The threadsail filefish, Stephanolepis cirrhifer, is an economically important marine species. However, wild catches have sharply decreased over the past 20 years, causing S. cirrhifer to be added to the IUCN Red List of Threatened Species. Accordingly, this study seeks to promote technological development for artificial breeding and early life-stage farming by defining the morphological characteristics of ontogenesis. The fertilized eggs, with a diameter of 0.62 ± 0.01 mm, were spherical and sticky and contained multiple oil globules of varying sizes. The embryonic development was observed and divided into eight phases, which were cleavage, blastocyst, gastrula, neurula, organogenesis, muscular contraction, heart pulsation, and hatching. At 3 days post-hatching (dph), the yolk sac was completely absorbed. The eye developed rapidly, and the mouth fissure and anus initially formed. Some larvae were fed on S-rotifers (Brachionus plicatilis). At 6–8 dph, the upper and lower jaws of larvae were gradually covered by leathery skin, and the head-to-body proportion increased. At 14–16 dph, the fin differentiation occurred in the dorsal, anal, and pectoral fins, with widespread distribution of yellow and melanin on the body surface. Swim bladder was clear. The swimming ability of larva was enhanced, resulting in an obvious clustering phenomenon. At 22–25 dph, the end of the notochord continued to tilt upwards, forming a tail fin. The trunk was evenly distributed with protruding circular punctate scales. The snout was covered with leathery epidermis, and the mouth began to round. At 40–45 dph, the juvenile completed metamorphosis, with horizontal dark stripes appearing on the trunk. Pigmented spots appeared on the tail fins. The counts of dorsal and anal fin spines were 34–36 and 32–34 dph, respectively. During the development of larvae and juveniles, the growth parameters, such as total length, standard length, body height, and body weight, were made as growth curves. The slopes of growth curves were calculated. We found two inflexion points occurring in the growth curves, which may be associated with metamorphosis and transitions in feeding habits. These results enrich the biological understanding of filefish species while providing guidance for artificial propagation and fry production in S. cirrhifer. Full article
(This article belongs to the Special Issue Early Development and Growth of Fishes: 2nd Edition)
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23 pages, 10335 KB  
Article
Multitemporal Spatial Analysis for Monitoring and Classification of Coal Mining and Reclamation Using Satellite Imagery
by Koni D. Prasetya and Fuan Tsai
Remote Sens. 2025, 17(6), 1090; https://doi.org/10.3390/rs17061090 - 20 Mar 2025
Cited by 1 | Viewed by 2101
Abstract
Observing coal mining and reclamation activities using remote sensing avoids the need for physical site visits, which is important for environmental and land management. This study utilizes deep learning techniques with a U-Net and ResNet architecture to analyze Sentinel imagery in order to [...] Read more.
Observing coal mining and reclamation activities using remote sensing avoids the need for physical site visits, which is important for environmental and land management. This study utilizes deep learning techniques with a U-Net and ResNet architecture to analyze Sentinel imagery in order to track changes in coal mining and reclamation over time in Tapin Regency, Kalimantan, Indonesia. After gathering Sentinel 1 and 2 satellite imagery of Kalimantan Island, manually label coal mining areas are used to train a deep learning model. These labelled areas included open cuts, tailings dams, waste rock dumps, and water ponds associated with coal mining. Applying the deep learning model to multitemporal Sentinel 1 and 2 imagery allowed us to track the annual changes in coal mining areas from 2016 to 2021, while identifying reclamation sites where former coal mines had been restored to non-coal-mining use. An accuracy assessment resulted in an overall accuracy of 97.4%, with a Kappa value of 0.91, through a confusion matrix analysis. The results indicate that the reclamation effort increased more than twice in 2020 compared with previous years’ reclamation. This phenomenon was mainly affected by the massive increase in coal mining areas by over 40% in 2019. The proposed method provides a practical solution for detecting and monitoring open-pit coal mines while leveraging freely available data for consistent long-term observation. The primary limitation of this approach lies in the use of medium-resolution satellite imagery, which may result in lower precision compared to direct field measurements; however, the ability to integrate historical data with consistent temporal coverage makes it a viable alternative for large-scale and long-term monitoring. Full article
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13 pages, 5666 KB  
Article
Research on the Dry Deep Flip-Flow Screening of Ilmenite and Its Pre-Throwing Tail Processing Technology
by Wei Shi, Weinan Wang, Pengfei Mao, Xu Hou, Songxue Zhang and Chenlong Duan
Minerals 2025, 15(3), 308; https://doi.org/10.3390/min15030308 - 16 Mar 2025
Cited by 1 | Viewed by 462
Abstract
Screening is a key step in the mineral process of ilmenite. As the grading particle size decreases, the phenomenon of clogged holes on the screening-plate intensifies, the screening environment deteriorates, and the screening effect deteriorates, seriously restricting subsequent sorting operations. This study proposes [...] Read more.
Screening is a key step in the mineral process of ilmenite. As the grading particle size decreases, the phenomenon of clogged holes on the screening-plate intensifies, the screening environment deteriorates, and the screening effect deteriorates, seriously restricting subsequent sorting operations. This study proposes a 1 mm dry flip-flow screening method for ilmenite to achieve efficient deep classification of fine-grained materials. Firstly, a laser displacement testing system is used to study the dynamic characteristics of the flip-flow screen; based on the characteristics of different feed particle sizes, further research is conducted on the 1 mm dry flip-flow screening effect of ilmenite under different ratios of obstructive particles and difficult-to-screen particles. The 1 mm screening effect can reach 85.41%. Finally, the pre-throwing tailings process based on 1 mm multi-stage screening is put forward. This pre-throwing tail process has the characteristic of not using water and is suitable for sorting in arid, water deficient, and high-altitude frozen soil areas. It has important promotion and application value. Full article
(This article belongs to the Special Issue Advances in the Theory and Technology of Physical Separation)
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20 pages, 3806 KB  
Article
Analysis and Research on the Flow Characteristics of Ice-Containing Filling Slurry Based on the Population Balance Model
by Mei Wang, Fan Zhang, Lang Liu, Guoming Wen, Wanying Ni and Deyang Kong
Energies 2025, 18(6), 1430; https://doi.org/10.3390/en18061430 - 13 Mar 2025
Viewed by 685
Abstract
In practical engineering applications, the cold storage functional backfill cooling system is prone to pipe clogging due to the agglomeration and crushing effects of the components of the ice particle-containing filling slurry. In addition, the fluidity of the slurry becomes more complex due [...] Read more.
In practical engineering applications, the cold storage functional backfill cooling system is prone to pipe clogging due to the agglomeration and crushing effects of the components of the ice particle-containing filling slurry. In addition, the fluidity of the slurry becomes more complex due to the change in the particle size distribution (PSD) during the pipeline transportation of the filling slurry, which limits the practical application effectiveness of the system. In order to promote the application and sustainable development of mining solid waste resources, a CFD–PBM coupling model was established to simulate the flow of the ice-containing filling slurry in horizontal circular tubes. On this basis, the effects of the initial ice content, inlet flow rate, initial particle size of tailings, and filling slurry concentration on the caking phenomenon during pipeline transportation were analyzed. The distribution of the pressure drop along the pipeline was also analyzed and calculated. The results show that the higher the flow velocity, the lower the slurry concentration, the larger the tailings’ particle size, the lower the ice content, and the lower the likelihood of agglomeration during transportation of the filling slurry. Full article
(This article belongs to the Section B: Energy and Environment)
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23 pages, 2278 KB  
Review
Nanosized Being of Ionic Surfactant Micelles: An Advanced View on Micellization Process
by Olga S. Zueva, Mariia A. Kazantseva and Yuriy F. Zuev
Colloids Interfaces 2025, 9(2), 15; https://doi.org/10.3390/colloids9020015 - 28 Feb 2025
Cited by 2 | Viewed by 1893
Abstract
An advanced model of ionic surfactant micellization has been developed. The structural and kinetic properties of micelles were analyzed in parallel from a universally accepted point of view and taking into account the principles of quantum mechanics, the phenomenon of ion pairing in [...] Read more.
An advanced model of ionic surfactant micellization has been developed. The structural and kinetic properties of micelles were analyzed in parallel from a universally accepted point of view and taking into account the principles of quantum mechanics, the phenomenon of ion pairing in the Stern layer, the symmetry considerations, and the chaos theory. It was shown that a micelle can be considered as a layered fullerene-like structure with a cavity in its center, possessing the solid-like properties of micelles in radial directions and the liquid-like properties in the perpendicular ones, allowing for water penetration between the surfactant head group and nearby methylene groups. The dimensions of the minimal fullerene-like structure formed by the terminal hydrogen atoms of surfactant methyl groups around the central cavity, unable to be occupied by surfactant tail fragments, were estimated. It was indicated that permanently occurring surfactant self-organization/disintegration needs a probabilistic description and revision of processes occurring in micellar systems built by ionic surfactants. It was noted that the probabilistic approach alters the mechanism of colloidal dissolution of hydrocarbon compounds and their solubilization by micelles. The advanced model proposes the same macroscopic properties of micelles as the classical one but modifies the structural characteristics of micelles on the nanoscale. Full article
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19 pages, 4475 KB  
Article
Mechanisms of Non-Aqueous Phase Liquid Retention in Low-Permeability Aquifer Lenses: Effects on Contaminant Remediation
by Weichao Sun, Shuaiwei Wang, Jianzhou Yu, Huimin Lin and Lin Sun
Water 2025, 17(4), 573; https://doi.org/10.3390/w17040573 - 17 Feb 2025
Viewed by 540
Abstract
The residual non-aqueous phase liquid (NAPL) within low-permeability lenses of aquifers is a major contributor to “tailing”, a phenomenon that complicates the remediation of NAPL-contaminated sites. A fundamental challenge in addressing this issue is the lack of understanding of the primary controlling factors [...] Read more.
The residual non-aqueous phase liquid (NAPL) within low-permeability lenses of aquifers is a major contributor to “tailing”, a phenomenon that complicates the remediation of NAPL-contaminated sites. A fundamental challenge in addressing this issue is the lack of understanding of the primary controlling factors and underlying effects of NAPL residuals in these aquifer lenses. This study aims to identify the key factors and mechanisms affecting NAPL residuals in low-permeability lenses through a series of experimental approaches. These include soil column simulation experiments on NAPL residuals in various low-permeability lenses, adsorption experiments on aquifer and lens particles, pore mercury intrusion testing, and particle size distribution analysis. The experiments provided valuable data on residual NAPL saturation SR, particle adsorption capacity, particle size, gradation, and pore size and distribution in different lenses. Using a mass conservation approach, the particle adsorption contributed less than 0.5% to the total NAPL residuals, while retention accounted for more than 99.5%, highlighting that retention is the dominant mechanism governing NAPL persistence in these lenses. The mechanism underlying this result was further clarified through an analysis of particle size characteristics. Correlation analysis was conducted to examine the relationships between residual NAPL and macropore porosity (nmax, diameter > 60 μm), mesopore porosity (nmid, diameter = 30~60 μm), and small pore porosity (nmin, diameter < 30 μm). The results demonstrated that mesopores exhibited the strongest correlation with NAPL retention, due to their pronounced capillary action and sufficient storage capacity for NAPL. Full article
(This article belongs to the Special Issue Soil and Groundwater Quality and Resources Assessment)
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18 pages, 8631 KB  
Article
Flow Characteristics and Pressure Pulsation Analysis of Cavitation Induced in a Double-Volute Centrifugal Pump
by Yongsha Tu, Xueying Zhao, Lifeng Lu, Wenjie Zhou, Siwei Li, Jin Dai, Zhongzan Wang, Yuan Zheng and Chunxia Yang
Water 2025, 17(3), 445; https://doi.org/10.3390/w17030445 - 5 Feb 2025
Cited by 2 | Viewed by 1348
Abstract
Cavitation is a complex multiphase flow phenomenon, and the generation of transient phase transitions between liquid and vapor during cavitation development leads to multi-scale vortex motion. The transient cavitation dynamics and centrifugal pump’s rotor–stator interaction will induce pressure fluctuations in the impeller and [...] Read more.
Cavitation is a complex multiphase flow phenomenon, and the generation of transient phase transitions between liquid and vapor during cavitation development leads to multi-scale vortex motion. The transient cavitation dynamics and centrifugal pump’s rotor–stator interaction will induce pressure fluctuations in the impeller and the volute fluid of the centrifugal pump, resulting in a complex flow field structure. Based on the Schnerr–Sauer cavitation model and SST k-ω turbulence model, this paper studies the transient characteristics of the cavitation-induced unsteady flow in the centrifugal pump and the excitation response to the pressure pulsation in the volute under different flow conditions, taking the large vertical double-volute centrifugal pump as the research object. The results indicate the following: As the impeller rotates, in the external excitation response, the jet-wake flow structure at the centrifugal pump blade outlet shows an increase in the blade frequency signal. This is evident near the measurement points of the volute tongue and separator. When severe cavitation occurs, the maximum amplitude at the blade frequency in the volute shifts from the pump tongue (30°) to the downstream of the tongue (45°). The value of fpmax is 3.1 times that when NPSHa = 8.88 m. By applying the Omega vortex identification method, it can be seen that the interaction between the vortices at the blade trailing edge and the stable vortex in the volute tongue undergoes a process of elongation, fusion, separation, and recovery. This represents the downstream influence of the impeller on the volute. When Q = 0.9Qd, the process of the blade passage vortex tail detaching and dissipating in the impeller flow path can be observed, demonstrating the upstream influence of the volute on the impeller. Full article
(This article belongs to the Special Issue Advanced Numerical Approaches for Multiphase and Cavitating Flows)
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21 pages, 10683 KB  
Article
Effect of Stress Aging on Strength, Toughness and Corrosion Resistance of Al-10Zn-3Mg-3Cu Alloy
by Dongchu Yang, Xi Zhao, Xianwei Ren, Shiliang Yan, Yihan Gao and Hongbin Liu
Materials 2025, 18(1), 181; https://doi.org/10.3390/ma18010181 - 3 Jan 2025
Cited by 2 | Viewed by 939
Abstract
The 7000 series aluminum alloy represented by Al-Zn-Mg-Cu has good strength and toughness and is widely used in the aerospace field. However, its high Zn content results in poor corrosion resistance, limiting its application in other fields. In order to achieve the synergistic [...] Read more.
The 7000 series aluminum alloy represented by Al-Zn-Mg-Cu has good strength and toughness and is widely used in the aerospace field. However, its high Zn content results in poor corrosion resistance, limiting its application in other fields. In order to achieve the synergistic improvement of both strength and corrosion resistance, this study examines the response of strength, toughness and corrosion resistance of a high-strength aluminum alloy tail frame under aging conditions with external stresses of 135 MPa, 270 MPa and 450 MPa. The results show that with the increase in the external stress level, the strength of the alloy improves, while its corrosion resistance decreases. An optimal balance of strength, toughness and corrosion resistance is achieved at the conditions of 270 MPa-120–24 h. This phenomenon can be attributed to two main factors: first, lattice defects such as vacancy and dislocation are introduced into the stress aging process. The introduction of a vacancy makes it easier for neighboring solute atoms to migrate there. This makes the crystal precipitates more dispersed. Also, the number of precipitates in the matrix increases from 2650 to 3117, and the size is refined from 2.96 nm to 2.64 nm. At the same time, the dislocation entanglement within the crystal structure promotes the dislocation strengthening mechanism and promotes the solute atoms to have enough channels for migration. Since too many dislocations can cause the crystal to become brittle and thus reduce its strength, entangled dislocations hinder the movement of the dislocations, thereby increasing the strength of the alloy. Secondly, under the action of external force, the precipitated phase is discontinuous, which hinders the corrosion expansion at the grain boundary, thus improving the corrosion resistance of the alloy. At low-stress states, the binding force of vacancy is stronger, the precipitation free zone (PFZ) is significantly inhibited, and the intermittent distribution effect of intergranular precipitates is the most obvious. As a result, the self-corrosion current decreases from 1.508 × 10−4 A∙cm−2 in the non-stress state to 1.999 × 10−5 A∙cm−2, representing an order of magnitude improvement. Additionally, the maximum depth of intergranular corrosion is reduced from 274.9 μm in the non-stress state to 237.7 μm. Full article
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17 pages, 3258 KB  
Article
Molecular Dynamics Insights into Peptide-Based Tetrodotoxin Delivery Nanostructures
by Shenghan Song, Xinyu Xia, Temair Shorty, Tongtong Li, Amy O. Stevens, Chao Zhao and Yi He
Molecules 2025, 30(1), 61; https://doi.org/10.3390/molecules30010061 - 27 Dec 2024
Cited by 1 | Viewed by 1360
Abstract
Tetrodotoxin (TTX), a potent Site-1 sodium channel blocker (S1SCB), offers highly effective local anesthetic properties with minimal addiction potential. To fully leverage TTX’s capabilities as a local anesthetic, it is crucial to develop a drug delivery system that balances its systemic toxicity with [...] Read more.
Tetrodotoxin (TTX), a potent Site-1 sodium channel blocker (S1SCB), offers highly effective local anesthetic properties with minimal addiction potential. To fully leverage TTX’s capabilities as a local anesthetic, it is crucial to develop a drug delivery system that balances its systemic toxicity with its therapeutic efficacy. Recent studies have shown that peptide mixtures, derived from fragments of Site-1 sodium channel proteins and enhanced with hydrophobic tails (designated MP1 and MP2), can self-assemble into nanostructures that exhibit remarkable sustained-release capabilities for TTX. Despite the profound impact that the addition of a hydrophobic tail has on altering the release behavior of the original peptides, the atomic-level interactions and mechanisms underlying this phenomenon remain poorly understood. In this study, a combination of ColabFold and molecular dynamics (MD) simulations were used to investigate the binding interactions between TTX and the nanostructures formed by MP1 and MP2 at an atomic level. Our findings agree with experimental observations and indicate that the MP1/MP2 nanostructure demonstrates greater stability and higher binding affinity for TTX compared to their non-modified counterparts, P1 and P2. The analysis of the simulations revealed that charged amino acids, specifically aspartic acid (ASP) and glutamic acid (GLU), on the peptides are crucial for strong TTX binding and serve as the primary functional sites. Additionally, the stability of the nanostructure significantly affects TTX binding affinity, elucidating why P1, P2, MP1, and MP2 exhibit different binding capabilities despite containing identical charged residues. The results reported here may provide fundamental information to drive future research and enhance the development of TTX-based drug delivery systems. Full article
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25 pages, 29734 KB  
Article
Study of Flow Characteristics and Anti-Scour Protection Around Tandem Piers Under Ice Cover
by Pengcheng Gao, Lei Chang, Xianyou Mou, Feng Gao, Haitao Su, Bo Zhang, Zhiqiang Shang, Lina Gao, Haode Qin and Hui Ma
Buildings 2024, 14(11), 3478; https://doi.org/10.3390/buildings14113478 - 31 Oct 2024
Cited by 1 | Viewed by 1012
Abstract
The impact of an ice-covered environment on the local flow characteristics of a bridge pier was studied through a series of flume tests, and the dominant factors affecting the scour pattern were found to grasp the change laws of the local hydrodynamic characteristics [...] Read more.
The impact of an ice-covered environment on the local flow characteristics of a bridge pier was studied through a series of flume tests, and the dominant factors affecting the scour pattern were found to grasp the change laws of the local hydrodynamic characteristics of the bridge pier under the ice cover. At the same time, because the scour problem of the pier foundation is a technical problem throughout the life-cycle of the bridge, to determine the optimal anti-scour protection effect on the foundation of the bridge pier, active protection scour plate was used to carry out scour protection tests, and its structural shape was optimized to obtain better anti-scour performance. The test results show that the jumping movements of sediment particles in the scour hole around the pier are mainly caused by events Q2 and Q4, which are accompanied by events Q1 and Q3 and cause the particle rolling phenomenon, where Q1 and Q3 events are outward and inward interacting flow regimes, and Q2 and Q4 events are jet and sweeping flow regimes, respectively. The power spectral attenuation rate in front of the upstream pier is high without masking effects, while strong circulation at the remaining locations results in strong vorticity and high spectral density, in particular, when the sampling time series is 60 s (i.e., f = 1/60), the variance loss rates under ice-covered conditions at the front of the upstream pier, between the two piers, and at the tail end of the downstream pier are 0.5%, 4.6%, and 9.8%, respectively, suggesting a smaller contribution of ice cover to the variance loss. Full article
(This article belongs to the Special Issue Advances in Soil-Structure Interaction for Building Structures)
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16 pages, 15478 KB  
Article
Natural Bacterial Co-Infection in Farmed European Sea Bass Intended for Experimental Research in Sicily, Southern Italy: Pathological Findings
by Simone Palazzolo, Claudio Gervasi, Jessica Maria Abbate, Emil Gjurčević, Rosa Falleti, Maria Giovanna Piro, Giovanni Lanteri, Carmelo Iaria and Fabio Marino
Fishes 2024, 9(9), 360; https://doi.org/10.3390/fishes9090360 - 13 Sep 2024
Cited by 1 | Viewed by 2230
Abstract
In mariculture facilities, bacterial infections pose significant production challenges, with potentially catastrophic impacts on fish species. Bacterial co-infections are a widespread phenomenon in the natural marine environment, although their impact on aquatic organisms remains poorly investigated. This study aimed to detail the pathological [...] Read more.
In mariculture facilities, bacterial infections pose significant production challenges, with potentially catastrophic impacts on fish species. Bacterial co-infections are a widespread phenomenon in the natural marine environment, although their impact on aquatic organisms remains poorly investigated. This study aimed to detail the pathological findings associated with a natural bacterial co-infection caused by three different pathogens, namely Photobacterium damselae subsp. piscicida, Tenacibaculum maritimum and Vibrio sp., as the cause of mass mortality in European sea bass. The fish had been reared in open-net cages in Sicily and later transferred for experimental research purposes to a user establishment after immunization with an inactivated vaccine. Macroscopic, cytological and histopathological examinations were performed on 109 animals, and bacterial species were identified by the 16S rRNA gene. Overall, ulcerative skin lesions, necrotizing myositis and tail rot with occasional tail loss were associated with tenacibaculosis and vibriosis, while P. damselae subsp. piscicida mainly caused granulomatous inflammation in the spleen and head kidney. Finally, an injection site reaction due to the oil-adjuvanted vaccine administered intraperitoneally was observed in the abdominal fat. Understanding the impact of bacterial pathogens is essential to manage the health and welfare of farmed fish, and the importance of a good health monitoring program cannot be overstated to avoid outbreaks and the possible emergence of new pathogens due to the intensification of the production systems, antibiotic resistance and climate changes. The study would also highlight the importance of the quarantine period when animals supplied for research come from aquaculture farms and how the main goal in the near future should be to better define the procedures to provide completely pathogen-free animals. Full article
(This article belongs to the Section Welfare, Health and Disease)
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