Search Results (991)

Thermophilic microorganisms represent an untapped reservoir of thermostable biocatalysts and stress-resilient biomolecules for industrial biotechnology. Aeribacillus pallidus, a Gram-positive moderate thermophile, has attracted attention for its enzymatic versatility and environmental adaptability, yet its genomic potential remains underexplored. Here, we present a comparative genomic analysis of 13 A. pallidus strains to uncover conserved and strain-specific traits relevant to biotechnology. Genomes ranged from 3.24 to 4.98 Mb, with GC content largely conserved (~39%) except for GS3372 (57.4%), indicating possible horizontal gene transfer. All strains encoded complete central metabolic pathways, while carbohydrate-active enzyme profiling revealed abundant glycoside hydrolases and glycosyltransferases, with GS3372 and MHI3390 enriched for lignocellulose-degrading enzymes. Secondary metabolite mining identified diverse biosynthetic gene clusters, including terpenes, sactipeptides, and bacteriocins, with PI8, W-12, and 8m3 exhibiting the greatest biosynthetic diversity. A core set of heat shock and universal stress proteins underscored robust thermotolerance. Phylogenomic and pan-genome analyses revealed high intraspecific diversity and an open pan-genome structure. Collectively, these findings position A. pallidus as a promising thermophilic chassis organism for sustainable applications, including biomass conversion, biofuel production, bioremediation, and the synthesis of heat-stable antimicrobial agents. Full article

To validate a positive relationship between air temperature (T) and atmospheric substances (S/G, a ratio of diffuse solar radiation to global solar radiation) found at four typical stations on the Earth, and a further investigation was conducted. Based on the analysis of long-term solar radiation, atmospheric substances, and air temperature at 29 representative stations of baseline surface radiation network (BSRN) in the world, the relationships and the mechanisms between air temperature and atmospheric substances were studied in more detail. A universal non-linear relationship between T and S/G was still found, which supported the previous relationship between T and S/G. This further revealed that a high (or low) air temperature is strongly associated with large (or small) amounts of atmospheric substances. The mechanism is that all kinds of atmospheric substances can keep and accumulate solar energy in the atmosphere and then heat the atmosphere, causing atmospheric warming at the regional and global scales. Therefore, it is suggested to reduce the direct emissions of all kinds of atmospheric substances (in terms gases, liquids and particles, and GLPs) from the natural and anthropogenic sources, and secondary formations produced from atmospheric compositions via chemical and photochemical reactions (CPRs) in the atmosphere, to slow down the regional and global warming through our collective efforts, by all mankind and all nations. Air temperature increased at most BSRN stations and many sites in China, and decreased at a small number of BSRN stations during long time scales, revealing that the mechanisms of air temperature change were very complex and varied with region, atmospheric substances, and the interactions between solar radiation, GLPs, and the land. Full article

Outdoor thermal comfort in tropical cities is increasingly threatened by rapid urbanization, high humidity, and insufficient climate-sensitive planning. Despite numerous studies on urban heat mitigation, there is a lack of empirical and numerical research that evaluates the synergistic application of bioclimatic strategies under humid tropical conditions. This paper addresses this gap by analyzing the combined effect of arborization, dry mist systems, water bodies, and sprinklers on outdoor thermal comfort at the Víctor Levi Sasso Campus of the Technological University of Panama. We hypothesized that synergistic application of these strategies provides greater thermal comfort improvements than isolated interventions. The central research question guiding this study was: To what extent can combined bioclimatic strategies enhance outdoor thermal comfort compared to individual strategies in humid tropical environments? To answer this, a hybrid methodology was employed, integrating ENVI-met dynamic simulations with in situ measurements and thermal comfort surveys based on the physiological equivalent temperature (PET) index and subjective comfort scales. The results demonstrate that combined strategies achieve superior reductions in mean radiant and surface temperatures while improving subjective comfort perceptions, highlighting their potential for context-sensitive urban design in tropical regions. Full article

Background: The adhesion between artificial teeth and denture bases is crucial for the longevity of complete dentures. This in vitro study evaluated the shear bond strength (SBS) and failure modes between artificial teeth and denture base resins produced with conventional, milled, and 3D-printed techniques. Materials: A total of 105 specimens were fabricated and assigned to 7 groups (n = 15) combining conventional, milled, or printed denture bases with conventional, milled, or printed teeth. SBS was tested using a universal testing machine, and failure modes were classified as adhesive, cohesive, or mixed. Data were analyzed with one-way ANOVA and Tukey’s post hoc test (α = 0.05). Results: SBS significantly varied among groups (p < 0.001). The conventional base–conventional tooth group (CB-CT) showed the highest bond strength (14.9 ± 3.69 MPa), while the printed base–milled tooth group (PB-MT) had the lowest (6.58 ± 3.41 MPa). Milled base groups showed intermediate values (11.7–12.4 MPa). Conclusions: Bond strength between denture teeth and denture bases depends on the fabrication workflow. Conventional heat-cured PMMA bases exhibited the most reliable adhesion, while milled bases demonstrated satisfactory performance with optimized bonding. Printed bases showed reduced and variable adhesion, suggesting the need for improved bonding protocols before their widespread clinical application in definitive prostheses. Full article

This paper evaluates Urban Heat Island (UHI) mitigation strategies in Florence’s historical centre, characterized by relevant cultural heritage value and significant tourist fluxes but increasingly susceptible to heatwaves. The research work focused on the evaluation of both current microclimate conditions and mitigation solutions for UHI-related issues, using ENVI-met microclimate modelling software as a simulation tool. Different models, featuring a 2 m grid resolution and detailed material properties, were produced to assess outdoor air temperature (Ta), mean radiant temperature (MRT), and Universal Thermal Climate Index (UTCI), chosen as reference parameters for human thermal sensation. Diversified conditions induced by the peculiarities of the urban layout were highlighted, with current Ta up to 32 °C and MRT exceeding 55 °C in paved open areas. Site-specific measures and their expected effectiveness were hence analyzed. De-paving and greening yield modest local cooling (Ta reduction up to −0.25 °C, MRT up to −1.75 °C), while tree installation ensures that MRT decreases by −7.50 °C to −12.00 °C. Most effectively, suspended shading fabrics preventing direct radiation can act on Ta (−0.09 °C to −0.25 °C) and provide substantial MRT reductions (−7.50 °C to −17.00 °C), significantly improving thermal comfort. The findings emphasize the potentialities of site-specific, reversible interventions in historic centres to combine climate adaptation and heritage preservation. Full article

The study explores long-term changes in the maximum number of consecutive hours per day of heat-related discomfort in Athens over the period 1960–2024, using the Universal Thermal Climate Index (UTCI). This index includes a four-category scale to represent heat stress intensity, ranging from ‘moderate’ to ‘extreme’, as part of its broader multi-category classification system. The analysis indicated a clear increase in the frequency of days with a large number of consecutive discomfort hours over the past decades. Almost 70% of the total number of days with 11 consecutive hours under at least ‘strong heat stress’ and 7 consecutive hours under at least ‘very strong heat stress’ were detected after the year 2000. Full article

Building energy consumption occupies an increasing proportion of the total energy consumption of society, and the use of energy-efficient windows can have great significance for energy saving. This case study examined the energy efficiency of various types of windows of the buildings on the University of New Brunswick campus, Fredericton, Canada. The energy performance of these windows was monitored by an infrared thermal camera from November 2021 to April 2022 and assessed in terms of the heat loss between different types of windows. The main findings were that (1) the temperature distribution of a window was strongly influenced by the indoor and outdoor temperature; (2) wood frame windows showed better insulation properties than metal frame ones; (3) fixed windows had a better energy performance than sliding windows and single-hung windows; and (4) the east orientation of a building and the use of Low-E glazing were the most effective expedients to reduce the winter energy required. By comparing these findings with earlier research, this study contributes new insights for cold climates, underscoreing the importance of Low-E glazing and configuration choice in building retrofits for energy-efficient, sustainable construction. Full article

A series of experiments were carried out to evaluate different anti-/de-icing approaches for a Pitot probe. Using the Iowa State University Icing Research Tunnel (ISU-IRT), this study compared the performance of a traditional electrically heated system with that of a hybrid concept combining reduced-power electrical heating and a superhydrophobic surface (SHS) coating. The effectiveness and energy efficiency of both methods were assessed. High-speed imaging was employed to capture the transient ice accretion and removal phenomena on the probe model under a representative glaze icing condition, while infrared thermography provided surface temperature distributions to characterize the unsteady heat transfer behavior during the protection process. Results indicated that, due to the placement of the internal resistive heating elements, ice deposits on the total pressure tube were easier to shed than those on the supporting structure. Relative to the conventional approach of maintaining a fully heated probe, the hybrid technique achieved comparable anti-/de-icing performance with substantially reduced power requirements—showing up to ~50% savings during anti-icing operation and approximately 30% lower energy use with 24% faster removal during de-icing. These findings suggest that the hybrid strategy is a promising alternative for improving Pitot probe icing protection. Full article

With the rapid development of distributed energy and electric vehicles (EVs), the limited hosting capacity of distribution networks has severely impacted their economic dispatch and safe operation. To address these challenges, in this work, an optimal planning model considering the uncertainty of wind and solar power output is proposed, aiming to determine the location and capacity of electric vehicle charging stations (EVCSs). The model seeks to minimize the total costs, voltage fluctuations, and network losses, subject to constraints such as EV user satisfaction and grid company satisfaction. A multi-objective heat exchange optimization algorithm under Gaussian mutation (MOTEO-GM) is employed to validate the model on an extended IEEE-33 bus system and a real-world case in the University Town area of Chenggong District, Kunming City. Simulation results indicate that, in the test system, voltage fluctuations and system power losses are decreased by 43.05% and 37.47%, respectively, significantly enhancing the economic operation of the distribution grid. Full article

This paper investigates the thermal performance of an office floor within the Faculty of Engineering at the British University in Egypt (BUE), located in Cairo, a city characterized by a hot arid climate. The study focuses on understanding the building’s thermal behavior by comparing two identical office rooms: Room 212 (north-facing) and Room 201 (south-facing). Utilizing dynamic thermal simulations with TRNSYS 18 for a full year, the research specifically analyzes the impact of these opposite orientations on indoor space temperature, total cooling loads, the monthly heat absorbed by various building surfaces, and the heat absorbed per unit area for each surface. The findings reveal significant disparities in thermal performance, particularly in terms of heat gain and cooling demand, directly attributable to orientation. This research highlights the critical role of facade orientation in mitigating radiative heat absorption and reducing energy consumption in educational buildings within hot climates, providing valuable insights for optimizing building design strategies to enhance thermal comfort and energy efficiency. Full article

This study investigates the external environmental temperature distribution of a small single-story BIPV building on a university campus in Jinan City, Shandong Province, China, under the most adverse summer high-temperature conditions. The temporal and spatial distribution characteristics and variation patterns of building external temperature are analyzed. The results indicated the following: (1) During summer high-temperature days, the peak temperature of the BIPV photovoltaic surface reached 52.4 °C, which is 17.4 °C higher than the ambient temperature. (2) External measurement points exhibited significant daytime heating (+2.86 °C) and nighttime cooling (average relative temperature increment of −1.52 °C). (3) Complex nonlinear temperature gradient variations existed within the 10–100 cm range from the surface, with localized heat accumulation occurring around 60 cm, where 77% of high-temperature days show temperature gradient anomalies. (4) Based on dimensionless analysis, a modified Richardson criterion for BIPV buildings is established: Ri < 0.3 represents building-geometry-dominated mechanisms, and Ri > 0.7 represents thermal-plume-dominated mechanisms. The critical values occur earlier than in classical theory. (5) Solar radiation and wind speed are key factors affecting temperature distribution, with more pronounced local heat accumulation under low-wind-speed conditions. This study provides scientific evidence for BIPV building performance optimization and environmental control. Full article

In a world of rapidly globalizing food markets, biodiversity, authenticity, and the safety of food products have become a universal concern. DNA barcoding is a widely used molecular-based method that can identify biological material and is used for the traceability of both raw materials and ingredients in processed food. In the present study, contacted within the framework of the BioValue Horizon Project, which promotes the role of agrobiodiversity in sustainable food systems, DNA barcoding using the ITS and rbcL markers was employed as a proof-of-concept approach to reveal the biodiversity and authenticity of ten commercial plant-based products. Following successful DNA amplification and sequencing using six products as a proof-of-concept, a diverse range of plant genera and species were identified, verifying biodiversity. A strong correlation between ITS and rbcL-based markers was demonstrated, supporting their combined use for reliable species-level biodiversity assessment. Finally, heat map analysis of label contents and sequencing-based genera identification confirmed high concordance between label claims and sequencing results in most cases, though undeclared species and absent labeled taxa were also detected, highlighting potential mislabeling or cross-contamination. Full article

Background Chronic low-back pain (LBP) is the largest contributor to disability worldwide, yet many assessments still reduce a complex, spatially distributed condition to a single 0–10 score. Body-map drawings capture location and extent of pain, but manual digitization is too slow and inconsistent for large studies or real-time telehealth. Methods Paper pain drawings from 332 adults in the multicenter COMEBACK study (four University of California sites, March 2021–June 2023) were scanned to PDFs. A Python pipeline automatically (i) rasterized PDF pages with pdf2image v1.17.0; (ii) resized each scan and delineated anterior/posterior regions of interest; (iii) registered patient silhouettes to a canonical high-resolution template using ORB key-points, Brute-Force Hamming matching, RANSAC inlier selection, and 3 × 3 projective homography implemented in OpenCV; (iv) removed template outlines via adaptive Gaussian thresholding, Canny edge detection, and 3 × 3 dilation, leaving only patient-drawn strokes; (v) produced binary masks for pain, numbness, and pins-and-needles, then stacked these across subjects to create pixel-frequency matrices; and (vi) normalized matrices with min–max scaling and rendered heat maps. RGB composites assigned distinct channels to each sensation, enabling intuitive visualization of overlapping symptom distributions and for future data analyses. Results Cohort-level maps replicated classic low-back pain hotspots over lumbar paraspinals, gluteal fold, and posterior thighs, while exposing less-recognized clusters along the lateral hip and lower abdomen. Neuropathic-leaning drawings displayed broader leg involvement than purely nociceptive patterns. Conclusions Our automated workflow converts pen-on-paper pain drawings into machine-readable digitized images and heat maps at the population scale, laying practical groundwork for spatially informed, precision management of chronic LBP. Full article

The molten salt reactor (MSR) is a prominent Generation IV nuclear reactor concept that offers substantial advantages over conventional solid-fueled systems, including enhanced fuel utilization, inherent passive safety features, and significant reductions in long-lived radioactive waste. Central to its safety strategy is a reliance on natural circulation (NC) mechanisms, which eliminate the need for active pumping systems and enhance system reliability during normal and off-normal conditions. However, the challenges associated with molten salts, such as their high melting points, corrosivity, and material compatibility issues, render experimental investigations inherently complex and demanding. Therefore, the use of high-Pr-number surrogate fluids represents a practical alternative for studying molten salt behavior under safer and more accessible experimental conditions. In this study, a single-phase natural circulation loop setup at the University of Idaho’s Thermal–Hydraulics Laboratory was employed to investigate NC behavior under various operating conditions. The RELAP5-3D code was initially validated against water-based experiments before employing Therminol-66, a high-Prandtl-number surrogate for molten salts, in the natural circulation loop for the first time. The RELAP5-3D results demonstrated good agreement with both steady-state and transient experimental results, thereby confirming the code’s ability to model NC behavior in a single-phase flow regime. The results also highlighted certain experimental limitations that should be addressed to enhance the NC loop’s performance. These include increasing the insulation thickness to reduce heat losses, incorporating a dedicated mass flow measurement device for improved accuracy, and replacing the current heater with a higher-capacity unit to enable testing at elevated power levels. By identifying and addressing the main causes of these limitations and uncertainties during water-based experiments, targeted improvements can be implemented in both the RELAP5 model and the experimental setup, thereby ensuring that tests using a surrogate fluid for MSR analyses are conducted with higher accuracy and minimal uncertainty. Full article

This article presents an experimental study on the hydrodynamics of coolant flow within the pressure vessel of a small modular reactor (SMR) cooled with water, including areas such as the annular downcomer, bottom chamber, and core-simulating channels that are being developed for use in land-based nuclear power plants. This paper describes the experimental setup and test model, measurement techniques used, experimental conditions under which this research was conducted, and results obtained. This study was conducted at the Nizhny Novgorod State Technical University (NNSTU) using a high-pressure aerodynamic testing facility and a scale model that included structural components similar to those found in loop-type reactors. Experiments were performed with Reynolds numbers (Re) ranging from 20,000 to 50,000 in the annular downcomer space of the test model. Two independent techniques were used to simulate the non-uniform flow field in the pressure vessel: passive impurity injection (adding propane to the airflow) and hot tracer (heating one of the reactor circulation loops). The axial velocity field at the inlet to the reactor core was also investigated. This study provided information about the spatial distribution of a tracer within the coolant flow in the annular downcomer and bottom chamber of the pressure vessel. Data on the distribution of the contrasting admixture are presented in plots. The swirling nature of the coolant flow within the pressurized vessel was analyzed. It was shown that the intensity of mixing within the bottom chamber of the pressure vessel is influenced by the presence of a central vortex. Parameters associated with the mixing of admixtures within the model for the pressure vessel were estimated. Additionally, the possibility for simulating flow with different temperature mixing processes using isothermal models was observed. Full article