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15 pages, 2507 KB  
Article
Heat Tolerance in Magallana hongkongensis: Integrative Analysis of DNA Damage, Antioxidant Defense, and Stress Gene Regulation
by Tuo Yao, Xiaodi Wang, Jie Lu, Shengli Fu, Changhong Cheng and Lingtong Ye
Antioxidants 2025, 14(9), 1075; https://doi.org/10.3390/antiox14091075 (registering DOI) - 2 Sep 2025
Abstract
Water temperature stands as a crucial environmental element, exerting an impact on the survival and growth of organisms in aquaculture. Heat stress poses a significant threat to the survival and aquaculture of the Hong Kong oyster Magallana hongkongensis (also known as Crassostrea hongkongensis [...] Read more.
Water temperature stands as a crucial environmental element, exerting an impact on the survival and growth of organisms in aquaculture. Heat stress poses a significant threat to the survival and aquaculture of the Hong Kong oyster Magallana hongkongensis (also known as Crassostrea hongkongensis), yet the underlying physiological and molecular mechanisms remain poorly understood. This study investigated the effects of elevated temperatures (35 °C and 37 °C) on survival, DNA damage, antioxidant enzyme activities, and gene expression related to apoptosis, inflammation, and heat shock proteins (HSPs) in M. hongkongensis. The median lethal temperature (LT50) of M. hongkongensis was determined to be 37.09 °C, with significant mortality observed at 35 °C compared with the control (29 °C). Antioxidant enzyme activities (SOD, CAT, and GPx) and T-AOC were up-regulated initially but exhibited divergent patterns under prolonged stress, indicating a temperature-dependent threshold for oxidative defense. Comet assay results also showed that heat stress induced severe DNA damage in hemocytes. Moreover, heat stress significantly up-regulated mRNA expression of apoptosis-related genes (Caspase-2, Caspase-8, Bax, and P53), inflammatory genes (TNF, p38-MAPK, and AP-1), and HSP family members (Hsp70, Hsp90, Hsp27, and Hsp68). The expression peaks of these genes were generally earlier and more pronounced at 37 °C, reflecting intensified cellular damage and protective responses. Collectively, this study demonstrates that M. hongkongensis employs integrated antioxidant, apoptotic, inflammatory, and HSP-mediated mechanisms to counteract heat stress, but temperatures exceeding 35 °C disrupt these defenses, leading to survival impairment. These findings provide critical insights into the heat adaptation strategies of M. hongkongensis and serve as a scientific foundation for developing sustainable aquaculture practices to mitigate summer heat stress. Full article
(This article belongs to the Special Issue Natural Antioxidants and Aquatic Animal Health—2nd Edition)
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21 pages, 2924 KB  
Article
Feasibility Study on Using Calcium Lignosulfonate-Modified Loess for Landfill Leachate Filtration and Seepage Control
by Jinjun Guo, Wenle Hu and Shixu Zhang
ChemEngineering 2025, 9(5), 96; https://doi.org/10.3390/chemengineering9050096 (registering DOI) - 2 Sep 2025
Abstract
Prolonged exposure to landfill leachate can weaken the impermeability of liner systems, leading to leachate leakage and the contamination of surrounding soil and water. To improve loess impermeability to enable its use as a liner material, this study uses synthetic landfill leachate to [...] Read more.
Prolonged exposure to landfill leachate can weaken the impermeability of liner systems, leading to leachate leakage and the contamination of surrounding soil and water. To improve loess impermeability to enable its use as a liner material, this study uses synthetic landfill leachate to investigate its effects on loess permeability via a series of laboratory tests. This study focused on the influence of varying dosages of calcium lignosulfonate (CLS) on loess permeability, along with its capacity to adsorb and immobilize heavy metal ions. Microscale characterization techniques, including Zeta potential analysis, X-ray fluorescence spectroscopy (XRF), and scanning electron microscopy (SEM), were employed to investigate the impermeability mechanisms of CLS-modified loess and its adsorption behavior toward heavy metals. The results indicate that the permeability coefficient of loess decreases significantly with increasing compaction, while higher leachate concentrations lead to a notable increase in permeability. At a compaction degree of 0.90, the permeability coefficient was reduced to 8 × 10−8 cm/s. In contrast, under conditions of maximum leachate concentration, the permeability coefficient rose markedly to 1.5 × 10−4 cm/s. Additionally, increasing the dosage of the compacted loess stabilizer (CLS) effectively reduced the permeability coefficient of the modified loess to 7.1 × 10−5 cm/s, indicating improved impermeability and enhanced resistance to contaminant migration. With the prolonged infiltration time of landfill leachate, the removal efficiency of Pb2+ gradually decreases and stabilizes, while the Pb2+ removal efficiency of the modified loess increased by approximately 40%. CLS-modified loess, through multiple mechanisms, reduces the fluid flow pathways and enhances its adsorption capacity for Pb2+, thereby improving the soil’s protection against heavy metal contamination. While these results demonstrate the potential of CLS-modified loess as a sustainable landfill liner material, the findings are based on controlled laboratory conditions with Pb2+ as the sole target contaminant. Future work should evaluate long-term performance under field conditions, including seasonal wetting–drying and freeze–thaw cycles, and investigate multi-metal systems to validate the broader applicability of this modification technique. Full article
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12 pages, 991 KB  
Article
An Integrated Sulfide Removal Approach from a Substrate for Biogas Production and the Simultaneous Production of Electricity
by Venko Beschkov, Ivan Angelov, Stefan Stefanov and Ljutzkan Ljutzkanov
Clean Technol. 2025, 7(3), 77; https://doi.org/10.3390/cleantechnol7030077 (registering DOI) - 2 Sep 2025
Abstract
Sulfides are frequently encountered in natural mineral water and different wastewater streams, and their presence significantly impedes subsequent water treatment or utilization. Sulfide removal, or at least its reduction, can be accomplished in different ways, but there is one straightforward method where sulfide [...] Read more.
Sulfides are frequently encountered in natural mineral water and different wastewater streams, and their presence significantly impedes subsequent water treatment or utilization. Sulfide removal, or at least its reduction, can be accomplished in different ways, but there is one straightforward method where sulfide is captured on a carbon-based sorbent, with the consequent sorbent regeneration producing electricity in liquid fuel cell mode. This multi-functional approach combines sulfide removal, energy generation, and water pre-treatment for various applications. The present work aims to show sulfide removal from sulfide-containing wastewater streams from alcohol and beverage manufacturing. The clean water could be used for biogas production. Sorbent regeneration was performed in fuel cell mode and was accompanied by electricity production. The experiments, conducted in a liquid-phase fuel cell, used electrode compartments that were separated by an anion-exchange membrane. Electroconductive charcoal, produced via the pyrolysis of residues from tire production and doped with zinc oxide, was used as a sorbent. The experimental treatments of vinasse, whey, and stillage for sulfide removal by this method show the sustainable performance of the sorbent for up to twelve consecutive runs. The biogas yield produced from vinasse was increased more than three times for the treated substrate compared to the reference case. Full article
(This article belongs to the Special Issue Latest Advances in Renewable Energy Technologies)
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12 pages, 986 KB  
Article
Structure–Optical Properties and Sustainability Assessment of Carbon Dots Derived from Laurus nobilis Leaves
by Valeria De Matteis, Cristina Baglivo, Silvia Tamborino, Mariafrancesca Cascione, Marco Anni, Paolo Vitali, Giuseppe Negro, Mariaenrica Frigione, Paolo Maria Congedo and Rosaria Rinaldi
Appl. Nano 2025, 6(3), 19; https://doi.org/10.3390/applnano6030019 (registering DOI) - 2 Sep 2025
Abstract
Carbon dots (CDs) derived from renewable biomass are emerging as sustainable alternatives to traditional nanomaterials for applications in bioimaging, sensing, and photonics. In this study, we reported a one-step synthesis of photoluminescent CDs from Laurus nobilis leaves particularly spread in the Mediterranean area. [...] Read more.
Carbon dots (CDs) derived from renewable biomass are emerging as sustainable alternatives to traditional nanomaterials for applications in bioimaging, sensing, and photonics. In this study, we reported a one-step synthesis of photoluminescent CDs from Laurus nobilis leaves particularly spread in the Mediterranean area. The resulting nanoparticles (NPs) exhibited average diameters of 3–5 nm and high colloidal stability in water. Structural analysis by X-Rays Diffraction revealed the presence of amorphous graphitic domains, while infrared spectroscopy confirmed oxygenated functional groups on the CD surface. Spectrofluorimetric analysis showed excitation-dependent blue–green emission with a maximum at 490 nm that can be applied also as label agents for cells. The environmental sustainability of the synthetic procedure was evaluated through a Life Cycle Assessment (LCA), highlighting that the current impacts were primarily associated with electricity consumption, due to the laboratory-scale nature of the process. These impacts are expected to decrease significantly with future scale-up and process optimization. Full article
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1506 KB  
Proceeding Paper
Fe3O4 Magnetic Biochar Derived from Pecan Nutshell for Arsenic Removal Performance Analysis Based on Fuzzy Decision Network
by Sasirot Khamkure, Chidentree Treesatayapun, Victoria Bustos-Terrones, Lourdes Díaz-Jimenéz, Daniella-Esperanza Pacheco-Catalán, Audberto Reyes-Rosas, Prócoro Gamero-Melo and Alejandro Zermeño-González
Eng. Proc. 2025, 107(1), 47; https://doi.org/10.3390/engproc2025107047 (registering DOI) - 1 Sep 2025
Abstract
This study evaluates Fe3O4 magnetic biochar synthesized from pecan nutshells for arsenic removal. Surface modification with Fe3O4 significantly enhanced arsenic adsorption selectivity and efficiency compared to raw biomass (PM). Synthesis variables (precursor type, particle size, Fe/precursor ratio, [...] Read more.
This study evaluates Fe3O4 magnetic biochar synthesized from pecan nutshells for arsenic removal. Surface modification with Fe3O4 significantly enhanced arsenic adsorption selectivity and efficiency compared to raw biomass (PM). Synthesis variables (precursor type, particle size, Fe/precursor ratio, N2) and adsorption conditions (such as concentration, pH, agitation) were investigated. The modified biochar achieved >90% arsenic removal efficiency under various conditions, demonstrating the modification’s critical role. A fuzzy decision network was employed to analyze experimental results and identify optimal conditions for maximizing performance. This approach effectively leverages knowledge for scenario-specific optimization, offering a sustainable strategy for advanced water treatment materials. Full article
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3 pages, 121 KB  
Editorial
Advances in Agricultural Irrigation Management and Technology
by Xingye Zhu
Water 2025, 17(17), 2591; https://doi.org/10.3390/w17172591 - 1 Sep 2025
Abstract
Agriculture is the cornerstone of global food security, yet water scarcity and inefficient irrigation practices pose significant challenges to sustainable production [...] Full article
(This article belongs to the Special Issue Advances in Agricultural Irrigation Management and Technology)
21 pages, 885 KB  
Review
Bibliometric Analysis of the Impact of Soil Erosion on Lake Water Environments in China
by Xingshuai Mei, Guangyu Yang, Mengqing Su, Tongde Chen, Haizhen Yang and Sen Wang
Water 2025, 17(17), 2592; https://doi.org/10.3390/w17172592 - 1 Sep 2025
Abstract
With the increasing attention to China’s ecological environment protection and the prominence of lake water environment problems, the impact of soil erosion on lake ecosystems has become an important research topic for regional sustainable development. Based on the CiteSpace bibliometric method, this study [...] Read more.
With the increasing attention to China’s ecological environment protection and the prominence of lake water environment problems, the impact of soil erosion on lake ecosystems has become an important research topic for regional sustainable development. Based on the CiteSpace bibliometric method, this study systematically analyzed 225 research articles on the impact of soil erosion on the water environment of lakes in China in the core collection of Web of Science from 1998 to 2025, aiming to reveal the research hotspots, evolution trends and regional differences in this field. The results show that China occupies a dominant position in this field (209 papers), and the Chinese Academy of Sciences is the core research institution (93 papers). The research hotspots show obvious policy-driven characteristics, which are divided into slow start periods (1998–2007), accelerated growth periods (2008–2015), explosive growth periods (2016–2020) and stable development periods (2021–2025). A keyword cluster analysis identified nine main research directions, including sedimentation effect (#0 cluster), soil loss (#2 cluster) and nitrogen and phosphorus migration (#11 cluster) in the Three Gorges Reservoir area. The study found that the synergistic effects of climate change and human activities (such as land use change) are becoming a new research paradigm, and the Yangtze River Basin, the Loess Plateau and the Yunnan–Guizhou Plateau constitute the three core research areas (accounting for 72.3% of the total literature). Future research should focus on a multi-scale coupling mechanism, a climate resilience assessment and an ecological engineering effectiveness verification to support the precise implementation of lake protection policies in China. This study provides a scientific basis for the comprehensive management of the soil erosion–lake water environment system, and also contributes a Chinese perspective to the sustainable development goals (SDG6 and SDG15) of similar regions in the world. Full article
(This article belongs to the Special Issue Soil Erosion and Soil and Water Conservation, 2nd Edition)
22 pages, 4626 KB  
Review
Biochar for Mitigating Nitrate Leaching in Agricultural Soils: Mechanisms, Challenges, and Future Directions
by Lan Luo, Jie Li, Zihan Xing, Tao Jing, Xinrui Wang and Guilong Zhang
Water 2025, 17(17), 2590; https://doi.org/10.3390/w17172590 - 1 Sep 2025
Abstract
Nitrate leaching from agricultural soils is a major contributor to groundwater contamination and non-point source pollution. Controlling this loss remains challenging due to the complexity of soil–water–nutrient interactions under intensive farming practices. Biochar, a porous, carbon-rich material derived from biomass pyrolysis, has emerged [...] Read more.
Nitrate leaching from agricultural soils is a major contributor to groundwater contamination and non-point source pollution. Controlling this loss remains challenging due to the complexity of soil–water–nutrient interactions under intensive farming practices. Biochar, a porous, carbon-rich material derived from biomass pyrolysis, has emerged as a promising amendment for nitrate mitigation. This review summarizes recent advances in understanding the roles of biochar in nitrate retention and transformation in soils, including both direct mechanisms—such as surface adsorption, ion exchange, and pore entrapment—and indirect mechanisms—such as enhanced microbial activity, soil structure improvement, and root system development. Field and laboratory evidence shows that biochar can reduce NO3-N leaching by 15–70%, depending on its properties, soil conditions, and application context. However, inconsistencies in performance due to differences in biochar types, soil conditions, and environmental factors remain a major barrier to widespread adoption. This review also suggests current knowledge gaps and research needs, including long-term field validation, biochar material optimization, and integration of biochar into precision nutrient management. Overall, biochar presents a multifunctional strategy for reducing nitrate leaching and promoting sustainable nitrogen management in agroecosystems. Full article
(This article belongs to the Special Issue Advanced Research in Non-Point Source Pollution of Watersheds)
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15 pages, 1435 KB  
Article
The Attribution Identification of Runoff Changes in the Kriya River Based on the Budyko Hypothesis Provides a Basis for the Sustainable Management of Water Resources in the Basin
by Sihai Liu and Kun Xing
Sustainability 2025, 17(17), 7882; https://doi.org/10.3390/su17177882 (registering DOI) - 1 Sep 2025
Abstract
Identifying the impact of climate change and changes in underlying surface conditions on river runoff changes is critical for sustainable water resource use and watershed management in arid regions. The Kriya River is not only a key support for water resources in the [...] Read more.
Identifying the impact of climate change and changes in underlying surface conditions on river runoff changes is critical for sustainable water resource use and watershed management in arid regions. The Kriya River is not only a key support for water resources in the arid environment of the Tarim Basin, but also a solid foundation for the survival and development of agricultural oases. In this study, the Kriya River Basin in Xinjiang, China, was taken as the research object, and the Mann–Kendall, Sen’s Slope, Cumulative Sum, and other methods were used to systematically analyze the temporal evolution law and multi-modal characteristics of runoff in the basin. Based on the Budyko hydrothermal coupling equilibrium equation, the contribution of temperature, evaporation, and the underlying surface to runoff variation was quantitatively interpreted. The study found that the annual runoff depth of the Kriya River Basin has shown a significant positive evolution trend in the past 60 years, with an increase rate of 0.5189 mm/a (p ≤ 0.01). Through the identification of mutation points, the runoff time series of the Kriya River was divided into the base period 1957–1999 and the change period 2000–2015. Without considering the supply of snowmelt runoff, the contribution rate of precipitation to runoff change was 75.23%, followed by the change in underlying surface (23.08%), and the potential evapotranspiration was only 1.69%. The results of this study provide a good scientific reference for water resources management and environmental governance in the Kriya River Basin. Full article
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16 pages, 2312 KB  
Article
Study on the Possibilities of Utilizing Wastes of Polymetallic Combines in South Kazakhstan for the Production of Composite Heavy Concrete
by Raushan Nurymbetova, Rayimberdy Ristavletov, Nikolay Suzev, Alexandr Kolesnikov, Elmira Kalshabekova, Medetbek Kambarov, Ruslan Kudabayev, Gulzhan Kopzhasarova, Berik Omarov, Zholdybay Zhumayev, Mermurat Nigmetov and Gulbanu Yesbolay
J. Compos. Sci. 2025, 9(9), 468; https://doi.org/10.3390/jcs9090468 (registering DOI) - 1 Sep 2025
Abstract
This article explores the use of waste from polymetallic combines in South Kazakhstan, specifically tailings from the Achisay and Ansay deposits, as aggregates (crushed stone, sand) and mineral additives (dispersed barite powder) for producing concrete with specified operational properties. These secondary raw materials [...] Read more.
This article explores the use of waste from polymetallic combines in South Kazakhstan, specifically tailings from the Achisay and Ansay deposits, as aggregates (crushed stone, sand) and mineral additives (dispersed barite powder) for producing concrete with specified operational properties. These secondary raw materials are now abundant in relation to their use, which makes them an affordable and accessible alternative for the manufacturing of concrete while also promoting environmental sustainability. X-ray diffraction, differential thermal analysis, and scanning electron microscopy of enriched barite ores in these tailings revealed valuable components, such as calcite, quartzite, and dolomite, suitable for use as aggregates and mineral additives. The calcite and quartzite content in the Ansay samples exceeds that in the Achisay samples. Concrete mixes with various proportions of crushed stone and sand from these tailings were prepared, and their working characteristics were analyzed. The impacts of filler content and grain composition on the characteristics of concrete mixtures were identified, and the requirements for optimizing aggregate grain composition to produce heavy concrete with desired qualities were determined. Heavy concrete with densities from 2300 to 2839 kg/m3 and compressive strengths from 41.6 to 58.2 MPa was developed. Physical and mechanical properties, including density, water absorption, frost resistance, and compressive strength, were also evaluated, confirming the feasibility of using technogenic waste in composite heavy concrete production. Full article
(This article belongs to the Special Issue From Waste to Advance Composite Materials, 2nd Edition)
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17 pages, 495 KB  
Conference Report
Innovative Solutions for a Sustainable Future: Main Topics of Selected Papers in the 19th SDEWES Conference in 2024
by Wenxiao Chu, Maria Vicidomini, Francesco Calise, Neven Duić, Poul Alberg Østergaard and Qiuwang Wang
Energies 2025, 18(17), 4647; https://doi.org/10.3390/en18174647 (registering DOI) - 1 Sep 2025
Abstract
From September 8th to 12th, 2024, the 19th SDEWES Conference on Sustainable Development of Energy, Water, and Environment Systems was successfully held in Rome. This event drew 700 researchers, scientists, and practitioners from 62 nations across six continents, with 570 participating in person [...] Read more.
From September 8th to 12th, 2024, the 19th SDEWES Conference on Sustainable Development of Energy, Water, and Environment Systems was successfully held in Rome. This event drew 700 researchers, scientists, and practitioners from 62 nations across six continents, with 570 participating in person and another 130 joining virtually. A total of seven papers were selected to be published in Energies, and the corresponding literature published in the most recent year is here reviewed. The main topics of the selected papers regard the adoption of district heating and cooling and their integration with renewable energies (such as geothermal or solar, the use of innovative bifacial PV panels, the use of biomass energy for the bio-synthetic natural gas production, the short-term electric load forecasting for industrial applications, and others. The reviewed papers show that several energy measures can be addressed to reach the decarbonization goals of 2050 and that the scientific community continues to find novel, sustainable, and efficient methods for the reduction in energy consumption and related CO2 emissions. Full article
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23 pages, 4537 KB  
Article
Assessing the Accuracy of Gridded Precipitation Products in the Campania Region, Italy
by Muhammad Shareef Shazil, Muhammad Aleem, Sheharyar Ahmad, Abdullah Abdullah and Roberto Greco
Water 2025, 17(17), 2585; https://doi.org/10.3390/w17172585 - 1 Sep 2025
Abstract
Accurate precipitation data are essential for hydrological modeling, climate studies, and water resource management. Indeed, there is an increasing focus on understanding shifts in precipitation events to monitor the risks of floods and droughts, as well as to ensure sustainable water resource management. [...] Read more.
Accurate precipitation data are essential for hydrological modeling, climate studies, and water resource management. Indeed, there is an increasing focus on understanding shifts in precipitation events to monitor the risks of floods and droughts, as well as to ensure sustainable water resource management. This study compares four reanalysis and satellite precipitation products (ERA5-Land, CHIRPS, PERSIANN, and TerraClimate) with ground data from 2003 to 2022. Among the datasets evaluated, ERA5-Land has the best performance (overall) in reproducing ground data, with a minimal mean bias error (MBE) of 1.91 mm, the highest correlation coefficient (R2 = 0.93), and the most favorable Nash–Sutcliffe efficiency (NSE = 0.93). In contrast, CHIRPS, PERSIANN, and TerraClimate significantly underestimate precipitation as compared to ground data. The categorical metrics also highlight ERA5-Land’s superior performance in identifying wet months. Spatial analysis shows that ERA5-Land and other datasets generally exhibit agreement regarding precipitation patterns. However, PERSIANN displays notable variances, particularly in northern regions, where it overestimates precipitation. To investigate possible changes in precipitation patterns, a longer period (1983–2022) is selected for trend analysis based on gridded precipitation products. Sen’s slope analysis does not reveal any significant annual precipitation trend. In autumn, the PERSIANN dataset indicates a significant increasing trend of +1.81 mm/year, which is also confirmed by ERA5-Land (+2.68 mm/year) and CHIRPS (+1.34 mm/year), although without statistical significance. The findings emphasize the need for more sophisticated satellite algorithms and integration with ground observations to improve precipitation accuracy. Full article
(This article belongs to the Section Hydrology)
32 pages, 11740 KB  
Article
Experimental and Analytical Study on Concrete Mechanical Properties of Recycled Carbon Fibers from Wind Turbine Blades
by Julita Krassowska
Materials 2025, 18(17), 4105; https://doi.org/10.3390/ma18174105 (registering DOI) - 1 Sep 2025
Abstract
This study examines the effects of incorporating recycled carbon fibers obtained from decommissioned wind turbine blades into cementitious composites. An extensive experimental program was carried out, varying fiber content (0–8 kg/m3), fiber length (25, 38, 50 mm), water-to-cement ratio (0.4, 0.5), [...] Read more.
This study examines the effects of incorporating recycled carbon fibers obtained from decommissioned wind turbine blades into cementitious composites. An extensive experimental program was carried out, varying fiber content (0–8 kg/m3), fiber length (25, 38, 50 mm), water-to-cement ratio (0.4, 0.5), and cement type (CEM I 42.5, CEM II 42.5R/A-V). The mechanical properties of the fiber-reinforced concretes, including compressive strength, flexural strength, splitting tensile strength, and modulus of elasticity, were evaluated. The addition of recycled carbon fibers significantly improved flexural and splitting tensile strengths, with increases exceeding 60% and 100%, respectively, at the highest fiber dosage (8 kg/m3), attributed to efficient crack-bridging capability. Compressive strength was mainly influenced by the water-to-cement ratio, while the modulus of elasticity showed slight reductions in some mixes due to fiber clustering and increased micro-porosity. Regression analysis indicated that shorter fibers (25 mm) were more effective in enhancing flexural strength, whereas longer fibers (50 mm) improved splitting tensile strength. Classical predictive models generally underestimated the flexural capacity of recycled-carbon-fiber-reinforced concretes, highlighting the need for recalibration. Optical microscopy confirmed uniform fiber dispersion at lower dosages and a dominant pull-out failure mechanism. The findings demonstrate the feasibility of using recycled carbon fibers to enhance the mechanical performance of concrete while supporting sustainability through waste diversion and circular economy strategies. Full article
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15 pages, 4009 KB  
Article
Towards the Potential of Using Downstream-Separated Solvents as the Pulping Liquor of Upstream Lignocellulose Fractionation for Enhanced Acetone–Butanol–Ethanol Production
by Changsheng Su, Yunxing Gao, Gege Zhang, Hao Wen, Rui Chen, Jiajing Wang, Yujie Li, Mingyuan Sun, Jikang Cao and Di Cai
Fermentation 2025, 11(9), 514; https://doi.org/10.3390/fermentation11090514 (registering DOI) - 1 Sep 2025
Abstract
Developing efficient, clean, and sustainable lignocellulose pretreatment technologies is essential for second-generation biofuel production. In this study, we attempted to use downstream-separated binary acetone-water, n-butanol-water, and ethanol-water solutions as the initial liquor for upstream organosolv pulping, in order to achieve the efficient [...] Read more.
Developing efficient, clean, and sustainable lignocellulose pretreatment technologies is essential for second-generation biofuel production. In this study, we attempted to use downstream-separated binary acetone-water, n-butanol-water, and ethanol-water solutions as the initial liquor for upstream organosolv pulping, in order to achieve the efficient and economic closed-circuit clean fractionation of the lignocelluloses for biological acetone–butanol–ethanol (ABE) production. Parameters, including concentration and temperature of the organosolv pulping, were optimized systematically. Results indicated that the 50 wt% ethanol and 30 wt% acetone aqueous solutions and pulping at 200 °C for 1 h exhibited better corn stover fractionation performances with higher fermentable sugar production. The total monosaccharide recovery (including glucose and xylose) was 50.92% and 50.89%, respectively, in subsequent enzymatic saccharification. While pulping corn stover using n-butanol solution as initial liquor showed higher delignification 86.16% (50 wt% of n-butanol and 200 °C for 1 h), the hydrolysate obtained by the organosolv pulps always exhibited good fermentability. A maximized 15.0 g/L of ABE with 0.36 g/g of yield was obtained in Ethanol-200 °C-50% group, corresponding to 112 g of ABE production from 1 kg of raw corn stover. As expected, the lignin specimens fractionated by closed-circuit organosolv pulping exhibited narrow molecule weight distribution, high purity, and high preservation of active groups, which supports further valorization. This novel strategy tightly bridges the upstream and downstream processes of second-generation ABE production, providing a new route for ‘energy-matter intensive’ and environmentally friendly lignocelluloses biorefineries. Full article
(This article belongs to the Special Issue Bioprocesses for Biomass Valorization in Biorefineries)
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16 pages, 1384 KB  
Article
Water Residues from Rosemary Essential Oil Production: Transforming Waste into a Potential Bioherbicide
by Federico Leoni, Francesco Nigro, Celia Duce, José González-Rivera, Marco Mattonai, Erika Ribechini, Paolo Bàrberi and Stefano Carlesi
Plants 2025, 14(17), 2717; https://doi.org/10.3390/plants14172717 - 1 Sep 2025
Abstract
Transforming industrial by-products into new resources is a fundamental principle of sustainable agriculture and circular bioeconomy. Waste products from rosemary (Rosmarinus officinalis L.) essential oil extraction, such as exhausted biomass and water residues (WRs), are rich in bioactive compounds like phenols and [...] Read more.
Transforming industrial by-products into new resources is a fundamental principle of sustainable agriculture and circular bioeconomy. Waste products from rosemary (Rosmarinus officinalis L.) essential oil extraction, such as exhausted biomass and water residues (WRs), are rich in bioactive compounds like phenols and terpenes. These by-products may represent a promising and economically viable option for agricultural management, particularly in weed control. This study evaluates the potential use of WR as a bioherbicide. In vitro experiments were conducted to assess the inhibitory effects of WR on the germination and seedling morphology (root and shoot development) of four detrimental weed species for temperate cropping systems: two monocotyledonous (Alopecurus myosuroides and Lolium multiflorum) and two dicotyledonous (Sinapis alba and Amaranthus retroflexus). WR was tested at four concentrations (0, 25, 50, and 100), corresponding to an increasing gradient of WR, with 100 representing pure WR. The results showed that WR did not significantly inhibit germination in A. myosuroides, L. multiflorum and S. alba, whereas A. retroflexus exhibited a dose-dependent inhibition, with germination reduced by 37.5%, 64.5%, and 91.6% at doses of 25, 50, and 100, respectively, compared with the control (dose 0). Furthermore, germination delays were observed across all tested species with promising application of WR for regulating weed–crop competitive interactions in the early crop growth stages. Results on the morphological traits of weed seedlings showed that WR application affected root more than shoot growth inhibition. In particular, WR demonstrated a pronounced root inhibitory effect in A. myosuroides, L. multiflorum, and A. retroflexus. In contrast, a dose-dependent increase in root length was observed for S. alba (21.41 mm at dose 0 and 25.77 mm, 30.97 mm and 35.96 mm, respectively, at doses 25, 50, and 100). The results of this study highlight the potential application of WR as a sustainable solution to be included in an integrated weed management (IWM) toolbox and underscore their role in promoting the valorization of waste from essential oil production. Full article
(This article belongs to the Section Phytochemistry)
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