Search Results (27)

Green leaf volatiles (GLVs) are key contributors to fruit aroma and plant defense, yet their accumulation during fruit ripening and across tissues remains understudied in Rosaceae berries. In this study, GLV profiles of the LOX-HPL pathway were compared between leaves, unripe, half-ripe, and ripe fruit of commercial strawberry (Fragaria × ananassa), raspberry (Rubus idaeus), blackberry (Rubus sp.), and woodland strawberry (Fragaria vesca) using HS-SPME–GC–MS. Eleven GLVs were identified, exhibiting strong species- and ripening-dependent variation. Odor activity value analysis showed that grassy aldehydes were the main predicted GLV contributors to aroma in ripe raspberry and blackberry samples, as opposed to fruity esters in ripe strawberry samples. However, differences in outdoor growth and harvest conditions should be considered when interpreting interspecies comparisons. Multivariate analyses suggest that GLVs may discriminate between ripening stages and reveal patterns of co-variation between (Z)-3-hexenal, (E,E)-2,4-hexadienal, and 4-oxohex-2-enal. Berry leaves contained substantially higher total GLVs and distinct compositions compared to fruit, such as the alcohol-rich profile of blackberry leaves, consistent with tissue- and species-associated differences in GLV profiles. Full article

Plant genotypes can vary in multiple functional traits due to adaptation to heterogenous environments. However, whether such variation can extrapolate to effects on soils and further on performance of subsequent plants, thus generating a genotypic variation in soil legacy, remains unclear. In this study, we studied how plant genotypic variation impacts soil legacy when exposed to aboveground insect herbivores. We used 11 wild genotypes of woodland strawberry (Fragaria vesca L.) experimentally exposed to leaf beetles (Galerucella tenella) to condition live soil. We then replaced the conditioning plants with naïve plants to examine soil legacy effects on growth and resistance on the subsequent plant genotype (referred to as the focal genotype) against the generalist herbivore Spodoptera littoralis. This allowed us to test the extent to which plant genotypic variation in soil legacy is altered by aboveground herbivory. We found an overall positive soil legacy effect of woodland strawberry, indicated by 69.9% higher belowground biomass of the subsequent focal genotype grown in conditioned soil compared to in unconditioned soil. We also observed a genotype-dependent soil legacy effect on performance of S. littoralis indicated as relative growth rates reduced by 37.9% on the subsequent focal genotype in soil conditioned by the focal genotype itself compared to by other genotypes, though the legacy effect was cancelled out when conditioning genotypes were exposed to G. tenella herbivory. A genotypic variation was further detected in soil legacy on the efficiency of conversion of ingested food by S. littoralis caterpillars feeding on the focal genotype. However, the genotypic variation was only present when the focal genotype was excluded from the conditioning genotypes at the exposure of G. tenella herbivory. Collectively, our study shows a conditional plant genotype-dependent soil legacy effect on herbivore resistance (measured as herbivore performance) rather than on plant growth, and the magnitude of the legacy effects depends on both the identity of the conditioning genotypes and the measures of the herbivore resistance. The findings of this study provide new insights into how plant genotypes or herbivory affects soil feedback on plant growth and herbivore resistance. Full article

Strawberry is an economically important horticultural crop cultivated worldwide. However, its growth, yield, and fruit quality are severely constrained by abiotic stresses, such as salinity, drought, and low temperature, as well as biotic stresses including pathogen attack and pest infestation. WRKY transcription factors (TFs) have been extensively characterized in model plants such as Arabidopsis and rice, and increasing evidence highlights their functional diversification and regulatory importance in horticultural crops, including tomato and grapevine. In this review, we summarize recent advances in understanding the roles of WRKY TFs in strawberry responses to both biotic and abiotic stresses, based on studies in both the diploid woodland strawberry (Fragaria vesca L.) and the octoploid cultivated strawberry (Fragaria × ananassa Duchesne). We discuss their involvement in hormone crosstalk, redox regulation, and transcriptional control within complex stress-response networks, while distinguishing expression-based associations from experimentally validated regulatory functions. To provide a clear framework for evaluating the current evidence, we categorize the findings according to a hierarchy of experimental validation, ranging from direct functional characterization in strawberry, to transient assays, heterologous systems (e.g., Arabidopsis or tobacco), transcriptomic inferences, and predictions based on sequence homology. Finally, we outline potential future directions for exploiting strawberry WRKY TFs as candidate regulators in molecular breeding, thereby providing a theoretical basis for future functional studies and breeding applications. Full article

Successful fertilization is essential for fruit bearing and yield enhancement, relying on male gametophyte which facilitates sexual reproduction by transferring the sperm cell to the ovule. To accomplish this task, pectate lyase is secreted to lubricate the sperm cell towards the female partner by different strategies. However, the specific impact of strawberry PL in male sterility and achene development remained elusive. Here, we systematically investigated the functions of diploid strawberry Fragaria vesca pectate lyase 1 (FvePL1), determining its localization in the cell wall and membrane. In situ hybridization presented its maximum expression in the anther, particularly the endothecium, connective tissue, and septum. Analysis of RNAi mutants and green fluorescent protein (GFP)-tagged overexpression lines demonstrated that the failure of FvePL1 significantly inhibited the fruit set due to stunted achenes. In addition, the deficiency of FvePL1 expression resulted in a 68.29% reduction in the number of pollen grains, a 73.27% decrease in pollen viability, morphological alterations of exine and intine, impaired pollen tube, and the inability of the sperm nucleus to reach its target due to the delayed and incomplete tapetal degeneration. In addition, the suppression of FvePL1 resulted in a 65.02% increase in survival rate withholding irrigation for 30 days, conferring enhanced drought tolerance by negatively influencing cell wall structure. Therefore, this study identified FvePL1 as a crucial regulator of pollen development, fertilization, and achene maturation and abiotic stress. These findings provide a framework for advancing research on the development of the male gametophyte in strawberry and even yield optimization in Rosaceous crops. Full article

Heat stress transcription factor (Hsf) families play important roles in abiotic stress responses. However, previous studies reported that HsfBs genes may play diverse roles in response to heat stress. Here, we conducted functional analysis on a woodland strawberry Class B Hsf gene, FvHsfB1a, to improve thermotolerance. The structure of FvHsfB1a contains a typical Hsf domain for DNA binding at the N-terminus, and FvHsfB1a belongs to the B1 family of Hsfs. The FvHsfB1a protein was localized in the nucleus. The FvHsfB1a gene was expressed in various strawberry tissues and highly induced by heat treatment. Under heat stress conditions, ectopic expression of FvHsfB1a in Arabidopsis improves thermotolerance, with higher germination and survival rates, a longer primary root length, higher proline and chlorophyll contents, lower malonaldehyde (MDA) and O²⁻ contents, better enzyme activities, and greater expression of heat-responsive and stress-related genes compared to WT. FvWRKY75 activates the promoter of the FvHsfB1a gene through recognizing the W-box element. Similarly, FvWRKY75-OE lines also displayed a heat-tolerant phenotype, exhibiting more proline and chlorophyll contents, lower MDA and O²⁻ contents, and higher enzyme activities under heat stress. Taken together, our study indicates that FvHsfB1a is a positive regulator of heat stress. Full article

The SAUR (Small Auxin-Upregulated RNA) family members are important early auxin responsive genes in plants, playing a key regulatory role in the auxin metabolism, signal transduction, plant organ development, and abiotic stress response. Auxin signaling is also crucial for strawberry fruit development, but its specific regulatory mechanism remains unclear. In this study, bioinformatics methods were used to systematically identify and evaluate the FvSAUR gene family members associated with the auxin signaling in strawberry. The woodland strawberry Yellow Wonder line ‘YW5AF7’ was used as the material to further investigate the expressional characteristics of FvSAUR members in response to the auxin signals. A total of 64 members of the SAUR gene family were identified in the woodland strawberry genome, associated with FvSAUR1-64. Further bioinformatics analysis revealed that the FvSAUR members have undergone significant structural differentiation during evolution, and their encoded proteins exhibit diversity in folding stability, physicochemical properties, and other aspects. The prediction of the cis-elements in the promoter sequences of these genes suggests that the FvSAUR genes may mediate multiple hormonal and environmental signals, participating in a wide range of biological processes. RNA seq data analysis combined with RT-qPCR analysis revealed a dynamic spatiotemporal expression pattern of the FvSAUR genes in the vegetative and reproductive organs of strawberries, particularly the high expression levels of FvSAUR11, 17, 19, 21, and other genes in flowers and young fruits, suggesting their potential regulatory roles in strawberry fruit development. Exogenous auxin treatment experiments further suggested that the expression of FvSAUR11 and FvSAUR19 is sensitive to the changes in auxin levels, indicating their potential involvement in auxin signal transduction during strawberry fruit development. Subcellular localization results showed that both proteins are located in the nucleus. The results of this study systematically analyzed the sequence structure characteristics, evolutionary history, expression patterns, and potential functions of the strawberry FvSAUR family members, providing important insights for further elucidating the roles of FvSAUR genes in strawberry fruit growth and development. Full article

Protein S-acyl transferases (PATs) are a family of enzymes that catalyze protein S-acylation, a post-translational lipid modification involved in protein membrane targeting, trafficking, stability, and protein–protein interaction. S-acylation plays important roles in plant growth, development, and stress responses. Here, we report the genome-wide analysis of the PAT family genes in the woodland strawberry (Fragaria vesca), a model plant for studying the economically important Rosaceae family. In total, 21 ‘Asp-His-His-Cys’ Cys Rich Domain (DHHC-CRD)-containing sequences were identified, named here as FvPAT1-21. Expression profiling by reverse transcription quantitative PCR (RT-qPCR) showed that all the 21 FvPATs were expressed ubiquitously in seedlings and different tissues from adult plants, with notably high levels present in vegetative tissues and young fruits. Treating seedlings with hormones indole-3-acetic acid (IAA), abscisic acid (ABA), and salicylic acid (SA) rapidly increased the transcription of most FvPATs. A complementation assay in yeast PAT mutant akr1 and auto-S-acylation assay of one FvPAT (FvPAT19) confirmed its enzyme activity where the Cys in the DHHC motif was required. An AlphaFold prediction of the DHHC and the mutated DHHC155S of FvPAT19 provided further proof of the importance of C155 in fatty acid binding. Together, our data clearly demonstrated that S-acylation catalyzed by FvPATs plays important roles in growth, development, and stress signaling in strawberries. These preliminary results could contribute to further research to understand S-acylation in strawberries and plants in general. Full article

Laccase (LAC, EC 1.10.3.2) is integral to the formation of lignin synthesis, flavonoid production, and responses to both biotic and abiotic stresses. While recent studies have characterized numerous LAC gene families and their functions across various plants, information regarding LAC genes in woodland strawberry (Fragaria vesca) remains limited. In this study, we identified a total of 57 FvLAC genes in the Fragaria vesca genome, which were phylogenetically categorized into five distinct groups. Analysis of the gene structures revealed a uniformity in the exon–intron structure among the subgroups, while conserved motifs identified unique motifs specific to certain subgroups, suggesting functional variations. Chromosomal localization studies indicated that FvLACs are distributed across seven chromosomes, and collinearity analysis demonstrated that FvLACs exhibit collinearity within the species. Additionally, cis-acting element analysis suggested that FvLAC genes are involved in stress responses, hormone responses, light responses, and the growth and development of plants. qRT-PCR demonstrated that FvLACs responded to salt, drought, and hormone stresses, with the expression levels of FvLAC24, FvLAC32, and FvLAC51 continuously increasing under these stress conditions. Furthermore, transgenic yeast experiments revealed that FvLAC51 enhanced yeast tolerance to both salt and drought stresses, while FvLAC24 and FvLAC32 negatively regulated yeast tolerance under these same conditions. These findings provide a theoretical foundation for further investigation into the functions of FvLAC genes in woodland strawberry. Full article

Nuclear factor Ys (NF-Ys) are heterotrimeric transcription factors that specifically bind to CCAAT boxes present in numerous eukaryotic promoters. In plants, NF-Y proteins consist of the following three subunits: NF-YA, NF-YB, and NF-YC, each encoded by a gene family. Accumulating evidence underscores the crucial roles of NF-Y proteins in various plant development processes and stress responses, such as embryogenesis, flowering time control, drought tolerance, and heat tolerance. Despite this, a comprehensive genome-wide overview of the NF-Y gene family in strawberries is lacking. To bridge this gap, this study was conducted to identify and characterize the NF-Ys in Fragaria vesca. The investigation revealed the presence of six NF-YA, twelve NF-YB, and five NF-YC members in F. vesca. Furthermore, a comprehensive analysis of the FveNF-Ys was performed, including their phylogenetic relationships, gene structures, chromosomal locations, and conserved domains. MiRNA target site prediction found that there were 30 miRNA target sites in 12 (52.2%) FveNF-Y genes. Additionally, the expression profiles of different tissues and developmental stages demonstrated tissue-specific expression patterns among certain members of each NF-Y subfamily. This observation suggests that specific NF-Y subfamily members may play unique roles in different tissues or stages of development. Furthermore, the transient expression assay demonstrated that three selected FveNF-Ys were localized in the nucleus. Our study represents a pioneering effort in the systemic analyses of FveNF-Y genes and will be useful in understanding the functional characterization of NF-Y genes in Fragaria species. Full article

Woodland strawberry (Fragaria vesca L.) is an underutilized plant species that could benefit from the use of biostimulants to improve crop productivity and fruit quality. The scope of the present study was to appraise the influence of two biostimulants (Trichoderma atroviride and plant protein hydrolysate)—used either alone or combined—on the plant performance and economic profitability of two woodland strawberry genotypes (‘Alpine’ and ‘Regina delle Valli’). Overall, data showed that ‘Alpine’ had the highest productive performances, whereas ‘Regina delle Valli’ revealed the highest fruit qualitative traits. T. atroviride inoculation and V-PH application significantly boosted plant marketable yield (+20.5% for T. atroviride and +12.9% for V-PH), total sugars (+1.9% for Trichoderma and +1.4% for V-PH) and anthocyanins (+14.1% for T. atroviride and +9.8% for V-PH) compared to non-treated plants. Plants supplied with both biostimulants had a higher marketable yield (+34.8%), mean fruit weight (+6.0%), fruit dry matter (+13.8%), total sugars (+3.5%), ascorbic acid (+12.7%), flavonoid (+26.3%) and anthocyanins (+29.9%) compared to non-treated plants. Furthermore, our study revealed that the highest fruit polyphenol concentration was recorded in both genotypes treated with the combination of biostimulants and in ‘Regina delle Valli’ sprayed with V-PH, whereas the highest antioxidant activity was found in ‘Regina delle Valli’ fruit when plants were supplied with both biostimulants. Our study pointed out that the application of microbial and non-microbial biostimulants, especially in combination, might be a useful strategy for improving the performances of underutilized species and, therefore, encouraging their cultivation, valorization and economic profitability (+6208.3 EUR /ha when plants were exposed to both biostimulants). Full article

Auxin is a crucial hormone that regulates various aspects of plant growth and development. It exerts its effects through multiple signaling pathways, including the TIR1/AFB-based transcriptional regulation in the nucleus. However, the specific role of auxin receptors in determining developmental features in the strawberry (Fragaria vesca) remains unclear. Our research has identified FveAFB5, a potential auxin receptor, as a key player in the development and auxin responses of woodland strawberry diploid variety Hawaii 4. FveAFB5 positively influences lateral root development, plant height, and fruit development, while negatively regulating shoot branching. Moreover, the mutation of FveAFB5 confers strong resistance to the auxinic herbicide picloram, compared to dicamba and quinclorac. Transcriptome analysis suggests that FveAFB5 may initiate auxin and abscisic acid signaling to inhibit growth in response to picloram. Therefore, FveAFB5 likely acts as an auxin receptor involved in regulating multiple processes related to strawberry growth and development. Full article

The cultivation of strawberries in controlled environments presents challenges related to environmental stressors, especially insufficient light. Melatonin, as a widely investigated plant growth regulator, was considered as a potential candidate to mitigate damage, and enhance photosynthesis stability. However, whether melatonin can improve photosynthesis under light deficiency in woodland strawberry (Fragaria vesca) remains elusive. In this study, we evaluated gas exchange parameters, Chlorophyll fluorescence parameters, photochemical efficiency, and the related genes’ expression levels to decipher the multifaceted impact of melatonin on photosynthesis. We found concentration-dependent effects of melatonin on photosynthetic parameters, with potential benefits at lower concentration and inhibitory effects at higher concentration. Notably, melatonin increased non-photochemical quenching (NPQ), a mechanism for dissipating excess light energy, while leaving photochemical quenching (qP) relatively stable. Further analysis showed that melatonin up-regulated key xanthophyll cycle-related genes (DHAR, VDE, and PsbS), indicating its involvement in energy dissipation processes. In conclusion, our study uncovered the dual and complex role of melatonin in the short-term response of photosynthesis in woodland strawberries under low-light conditions. Full article

MYB and BBX transcription factors play important roles in flavonoid biosynthesis. Here, we obtained transgenic woodland strawberry with stable overexpression of FaMYB5, demonstrating that FaMYB5 can increase anthocyanin and proanthocyanidin content in roots, stems and leaves of woodland strawberry. In addition, bimolecular fluorescence complementation assays and yeast two-hybridization demonstrated that the N-terminal (1-99aa) of FaBBX24 interacts with FaMYB5. Transient co-expression of FaBBX24 and FaMYB5 in cultivated strawberry ‘Xiaobai’ showed that co-expression strongly promoted the expression of F3′H, 4CL-2, TT12, AHA10 and ANR and then increased the content of anthocyanin and proanthocyanidin in strawberry fruits. We also determined that FaBBX24 is also a positive regulator of anthocyanin and proanthocyanidin biosynthesis in strawberry. The results reveal a novel mechanism by which the FaMYB5–FaBBX24 module collaboratively regulates anthocyanin and proanthocyanidin in strawberry fruit. Full article

The strawberry (Fragaria × ananassa Duch.) is an important horticultural crop that is widely grown all over the world. Its sweetness, aroma, nutritional value and bright color make it popular. The woodland strawberry (Fragaria vesca) is a model plant for studying non-climacteric fruits because its respiration rate does not change significantly during fruit ripening. The B-box (BBX) protein family is made up of zinc-finger transcription factors important in plant growth and development. In this study, we identified 22 FveBBX genes from the newly released woodland strawberry genome database by comprehensive bioinformatics analysis. Phylogenetic analysis divided these FveBBX genes into five subfamilies. A promoter cis-acting element analysis detected 29 elements related to plant development, light response, abiotic stress and hormone response in the promoter of FveBBX genes. According to transcriptome data, relatively few BBX genes had tissue-specific expression, with examples including FveBBX12, which was expressed only in pre-fertilization cortex and pitch, and FveBBX19, which was specifically expressed in mature anthers. During fruit ripening, the expressions of eight FveBBX genes decreased by more than two-fold, and three FveBBX gene expressions increased more than two-fold both in “Ruegen” and “Yellow Wonder”. After cold and heat stresses, around half of the FveBBX genes displayed altered expression, especially FveBBX16 which showed an 8.3-fold increase in expression after heat treatment, while FveBBX14 showed at least an 11-fold decrease in expression after cold treatment. According to the result of quantitative real-time PCR (qRT-PCR), FveBBX genes’ expression differed depending on the photoperiod. Notably, FveBBX7 gene expression was the opposite during the first 16 h of the long-day (LD) and short-day (SD) conditions. This study provides helpful information for further research on BBX gene activity of the woodland strawberry in plant growth and development and adaptation to temperature and photoperiod environmental conditions. Full article

Protein phosphatase 2C (PP2C) is a negative regulator of serine/threonine residue protein phosphatase and plays an important role in abscisic acid (ABA) and abiotic-stress-mediated signaling pathways in plants. The genome complexity of woodland strawberry and pineapple strawberry is different due to the difference in chromosome ploidy. This study conducted a genome-wide investigation of the FvPP2C (Fragaria vesca) and FaPP2C (Fragaria ananassa) gene family. Fifty-six FvPP2C genes and 228 FaPP2C genes were identified from the woodland strawberry and pineapple strawberry genomes, respectively. FvPP2Cs were distributed on seven chromosomes, and FaPP2Cs were distributed on 28 chromosomes. The size of the FaPP2C gene family was significantly different from that of the FvPP2C gene family, but both FaPP2Cs and FvPP2Cs were localized in the nucleus, cytoplasm, and chloroplast. Phylogenetic analysis revealed that 56 FvPP2Cs and 228 FaPP2Cs could be divided into 11 subfamilies. Collinearity analysis showed that both FvPP2Cs and FaPP2Cs had fragment duplication, and the whole genome duplication was the main cause of PP2C gene abundance in pineapple strawberry. FvPP2Cs mainly underwent purification selection, and there were both purification selection and positive selection effects in the evolution of FaPP2Cs. Cis-acting element analysis found that the PP2C family genes of woodland and pineapple strawberries mainly contained light responsive elements, hormone responsive elements, defense and stress responsive elements, and growth and development-related elements. The results of quantitative real-time PCR (qRT-PCR) showed that the FvPP2C genes showed different expression patterns under ABA, salt, and drought treatment. The expression level of FvPP2C18 was upregulated after stress treatment, which may play a positive regulatory role in ABA signaling and abiotic stress response mechanisms. This study lays a foundation for further investigation on the function of the PP2C gene family. Full article