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Molecules 2012, 17(12), 14975-14994; doi:10.3390/molecules171214975

The Role of Slingshot-1L (SSH1L) in the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Cardiomyocyte-Like Cells

1
Department of Orthopaedics, China-Japan Union Hospital of Jilin University, Changchun 130033, China
2
The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130033, China
3
Research Center for Gene Therapy, China-Japan Union Hospital of Jilin University, Changchun 130033, China
*
Author to whom correspondence should be addressed.
Received: 7 November 2012 / Revised: 11 December 2012 / Accepted: 12 December 2012 / Published: 17 December 2012
Download PDF [1111 KB, 18 June 2014; original version 18 June 2014]

Abstract

Adult cardiomyocytes (CMs) have very limited capacity to regenerate. Therefore, there is a great interest in developing strategies to treat infarcted CMs that are able to regenerate cardiac tissue and promote revascularization of infarcted zones in the heart. Recently, stem cell transplantation has been proposed to replace infarcted CMs and to restore the function of the affected tissue. This area of research has become very active in recent years due to the huge clinical need to improve the efficacy of currently available therapies. Slingshot (SSH) is a family of protein phosphatases, which can specifically dephosphorylate and reactivate cofilin and inhibit the polymerization of actin filaments and actively involved in cytoskeleton rearrangement. In this study, we found that SSH1L promoted morphology changes of microfilaments during differentiation but was inhibited by the inhibitors of actin polymerization such as cytochalasin D. Overexpression of SSH1L could promote cardiac-specific protein and genes expression. 5-Aza can induce the differentiation of hMSCs into cardiomyocyte-like cells in vitro. We also observed that SSH1L efficiently promotes hMSCs differentiation into cardiomyocyte-like cells through regulation and rearrangement of cytoskeleton. Our work provides evidence that supports the positive role of SSH1L in the mechanism of stem cell differentiation into cardiomyocyte-like cells.
Keywords: mesenchymal stem cells; cardiomyocyte-like cells; 5-azacytidine; Slingshot-1L mesenchymal stem cells; cardiomyocyte-like cells; 5-azacytidine; Slingshot-1L
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Zhao, J.-W.; Zhang, M.-R.; Ji, Q.-Y.; Xing, F.-J.; Meng, L.-J.; Wang, Y. The Role of Slingshot-1L (SSH1L) in the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Cardiomyocyte-Like Cells. Molecules 2012, 17, 14975-14994.

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