Sodium Valproate Induces Cell Senescence in Human Hepatocarcinoma Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. SVP Inhibits the Proliferation of Bel-7402 Cells
2.2. SVP Activates SA-β-gal in Bel-7402 Cells
2.3. SVP Arrests Bel-7402 Cells in G0/G1 Phase
2.4. Effects of SVP on the Expression of Senescence Associated Genes
2.5. SVP Induces Hyperacetylation of Histone H3 and H4
2.6. SVP Induces Cell Senescence in Bel-7404 Cells
2.7. SVP Induces p21 Expression and Inhibits RB Phosphorylation in Bel-7404 Cells
2.8. Discussion
3. Experimental
3.1. Chemicals and Reagents
3.2. Cell Culture
3.3. Cell Proliferation Assay
3.4. Senescence-Associated β-Galactosidase Staining
3.5. Flow Cytometric Analysis
3.6. Western Blot
3.7. Chromatin Immunoprecipitation Assay
3.8. Statistical Analyses
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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An, H.-M.; Xue, Y.-F.; Shen, Y.-L.; Du, Q.; Hu, B. Sodium Valproate Induces Cell Senescence in Human Hepatocarcinoma Cells. Molecules 2013, 18, 14935-14947. https://doi.org/10.3390/molecules181214935
An H-M, Xue Y-F, Shen Y-L, Du Q, Hu B. Sodium Valproate Induces Cell Senescence in Human Hepatocarcinoma Cells. Molecules. 2013; 18(12):14935-14947. https://doi.org/10.3390/molecules181214935
Chicago/Turabian StyleAn, Hong-Mei, Yong-Fei Xue, Yan-Li Shen, Qin Du, and Bing Hu. 2013. "Sodium Valproate Induces Cell Senescence in Human Hepatocarcinoma Cells" Molecules 18, no. 12: 14935-14947. https://doi.org/10.3390/molecules181214935