Brefeldin A Effectively Inhibits Cancer Stem Cell-Like Properties and MMP-9 Activity in Human Colorectal Cancer Colo 205 Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Colo 205 Suspension Cells Were Sensitive to the Cytotoxic Effect of BFA
2.2. BFA Reduced the Clonogenicity of Colo 205 CSCs
2.3. BFA Induced Apoptosis of Suspension Colo 205 cells
2.4. BFA Induced ER Stress Response in Suspension Colo 205 Cells at Nanogram per Milliliter Range
2.5. BFA Reduced the Activity of MMP9
2.6. Discussion
3. Experimental
3.1. Cell Culture
3.2. Cell Survival Assay
3.3. Soft Agar Colony Formation Assay
3.4. Determination of Sub-G1 Population and Annexin V/PI Staining
3.5. Reverse-Transcription Polymerase Chain Reaction (RT-PCR)
3.6. Gelatin Zymography
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Tseng, C.-N.; Huang, C.-F.; Cho, C.-L.; Chang, H.-W.; Huang, C.-W.; Chiu, C.-C.; Chang, Y.-F. Brefeldin A Effectively Inhibits Cancer Stem Cell-Like Properties and MMP-9 Activity in Human Colorectal Cancer Colo 205 Cells. Molecules 2013, 18, 10242-10253. https://doi.org/10.3390/molecules180910242
Tseng C-N, Huang C-F, Cho C-L, Chang H-W, Huang C-W, Chiu C-C, Chang Y-F. Brefeldin A Effectively Inhibits Cancer Stem Cell-Like Properties and MMP-9 Activity in Human Colorectal Cancer Colo 205 Cells. Molecules. 2013; 18(9):10242-10253. https://doi.org/10.3390/molecules180910242
Chicago/Turabian StyleTseng, Chao-Neng, Chien-Fu Huang, Chung-Lung Cho, Hsueh-Wei Chang, Chao-Wei Huang, Chien-Chih Chiu, and Yung-Fu Chang. 2013. "Brefeldin A Effectively Inhibits Cancer Stem Cell-Like Properties and MMP-9 Activity in Human Colorectal Cancer Colo 205 Cells" Molecules 18, no. 9: 10242-10253. https://doi.org/10.3390/molecules180910242