Design and Synthesis of New 2-Aryl-4,5-Dihydro-thiazole Analogues: In Vitro Antibacterial Activities and Preliminary Mechanism of Action

Abstract

Sixty 2-aryl-4,5-dihydrothiazoles were designed and synthesized in yields ranging from 64% to 89% from cysteine and substituted-benzonitriles via a novel metal- and catalyst-free method. The structures of the title compounds were confirmed mainly by NMR spectral data analysis. Antibacterial activity assays showed that the compounds (S)-2-(2′-hydroxyphenyl)-4-hydroxy-methyl-4,5-dihydrothiazole (7h) and (R)-2-(2′-hydroxyphenyl)-4-hydroxymethyl-4,5-dihydro-thiazole (7h′) exhibited significant inhibition against Ralstonia solanacearum, Pseudomonas syringae pv. actinidiae, Bacillus subtilis and Bacillus cereus, with minimum inhibitory concentrations (MICs) ranging from 3.91 to 31.24 μg·mL⁻¹. The effect of substituents showed that not only electron-withdrawing groups, but also electron-donating groups could abolish the antibacterial activities unless a 2′-hydroxy group was introduced on the 2-aryl substituent of the 4,5-dihydrothiazole analogues. The results of scanning electron microscope (SEM) and fatty acid exposure experiments indicated that these antibacterial compounds influence fatty acid synthesis in the tested bacteria.

1. Introduction

Ralstonia solanacearum and Pseudomonas syringae pv. Actinidiae are the causes of serious bacterial diseases that pose a major threat to agricultural production worldwide [1]. Unfortunately, there have been few reports of effective bactericides against these pathogens. In the process of screening new agricultural bactericides, we found that some 2-arylthiazolines such as (S)-2-(2′-hydroxyphenyl)-4-hydroxymethyl-4,5-dihydrothiazole and (R)-2-(2′-hydroxyphenyl)-4-hydroxymethyl-4,5-dihydrothiazole exhibited potent antibacterial activities [2,3,4,5]. Inspired by these results, we wondered whether the 2-arylthiazoline scaffold could be a lead compound for agricultural bactericides. In order to investigate the bactericidal activities and clarify the inevitable structural motifs of 2-arylthiazoline analogues, sixty 2-aryl-4,5-dihydrothiazoles were then designed and synthesized.

A couple of protocols have been developed for the synthesis of 2-arylthiazoline scaffolds, which mainly include using ethyl benzimidates reacted with cysteine [6], aryl nitriles condensed with cysteine in buffered media [7], Ru-catalyzed/TBHP oxidation reactions [8], and so on [9,10,11]. The broad application of these methods has been hindered by the following drawbacks: long reaction time, high reaction temperatures (>100 °C), tedious work-ups and the use of expensive noble metal catalysts.

We present herein the design and synthesis a series of 2-aryl-4,5-dihydrothiazolines under mild metal- and catalyst-free conditions [12] as well as their in vitro antibacterial activities against Ralstonia solanacearum (R. solanacearum), Pseudomonas syringae pv. Actinidiae (P. syringae), Bacillus subtilis (B. subtilis) and Bacillus cereus (B. cereus). Furthermore, we also explore the preliminary antibacterial mechanism of compound 7h (Figure 1).

Figure 1. Chemical structures of (S)-2-(2-hydroxyphenyl)-4-hydroxymethyl-4,5-dihydrothiazole and (R)-2-(2-hydroxyphenyl)-4-hydroxymethyl-4,5-dihydrothiazole.

Figure 1. Chemical structures of (S)-2-(2-hydroxyphenyl)-4-hydroxymethyl-4,5-dihydrothiazole and (R)-2-(2-hydroxyphenyl)-4-hydroxymethyl-4,5-dihydrothiazole.

2. Results and Discussion

2.1. Preparation of 2-Arylthiazoline Analogues

The synthetic route to the 2-arylthiazoline analogues is depicted in Scheme 1. Aryl nitriles and methyl cysteine were used as starting materials and reacted in dry methanol in the presence of sodium carbonate and refluxed for 12–15 h to obtain methyl-2-aryl-4,5-dihydrothiazole-4-carboxylates in yields ranging from 64% to 89% [12].

Scheme 1. Synthetic route of 2-arylthiazoline analogues.

Scheme 1. Synthetic route of 2-arylthiazoline analogues.

The results suggested that ortho-substituents did not favor the reaction, in particular larger groups, such as -Br and -Cl. Meanwhile the electron-withdrawing groups were better in this reaction than electron-donating ones. The carboxylates 1e–10e, 1e′–10e′ were reduced by NaBH₄ in EtOH to furnish the corresponding 2-aryl-4-hydroxymethyly-4,5-dihydrothiazoles 1h–10h, 1h′–10h′ and treated with 10% sodium hydroxide solution in EtOH to get the corresponding 2-aryl-4,5-dihydrothiazole-4-carboxylic acids 1a–10a, 1a′–10a′. The structures of all the compounds were confirmed mainly by analyses of their NMR spectral and mass spectrometry data or reference to related literature [3,13,14,15].

2.2. Antibacterial Activity Assays

All compounds were evaluated for in vitro antibacterial activities against two Gram-positive bacteria—B. cereus and B. subtilis—and two Gram-negative bacteria—R. solanacearum and P. syringae. The inhibition zone diameters and minimum inhibitory concentrations (MICs), presented in Table 1 and Table 2, were determined by the filter paper and double-dilution methods, respectively. However, only six 2′-hydroxyphenyl-4,5-dihydro-thiazoles showed potent antibacterial activities at the tested concentration, in particular, compounds 7h and 7h′ showed the highest antibacterial activities for all tested bacteria.

Table 1. Inhibition Zone Diameters of Six Compounds against the Tested Bacteria.

Compounds	Diameter of Inhibition Zone (mm) in 10 μL/Disk (Mean ± S.D.)
	B. subtilis	B. cereus	R. solanacearum	P. syringae
7a	-	-	-	11.0 ± 0.23 (+)
7a′	-	-	-	10.5 ± 0.56 (+)
7e	12.3 ± 0.02 (+)	-	9.1 ± 0.11 (++)	-
7e′	11.2 ± 0.45 (+)	-	9.6 ± 0.43 (++)	-
7h	20.5 ± 0.56 (+++)	16.3 ± 0.23 (+++)	15.5 ± 0.13 (++)	18.2 ± 0.34 (+++)
7h′	20.0 ± 0.22 (+++)	16.7 ± 0.11 (+++)	14.9 ± 0.55 (++)	17.8 ± 0.23 (+++)

All values were means of three replicates, “+++” means transparent; “++” means clear; “+” means visible; “−” means no inhibitory effect.

Table 1. Inhibition Zone Diameters of Six Compounds against the Tested Bacteria.

Compounds	Diameter of Inhibition Zone (mm) in 10 μL/Disk (Mean ± S.D.)
	B. subtilis	B. cereus	R. solanacearum	P. syringae
7a	-	-	-	11.0 ± 0.23 (+)
7a′	-	-	-	10.5 ± 0.56 (+)
7e	12.3 ± 0.02 (+)	-	9.1 ± 0.11 (++)	-
7e′	11.2 ± 0.45 (+)	-	9.6 ± 0.43 (++)	-
7h	20.5 ± 0.56 (+++)	16.3 ± 0.23 (+++)	15.5 ± 0.13 (++)	18.2 ± 0.34 (+++)
7h′	20.0 ± 0.22 (+++)	16.7 ± 0.11 (+++)	14.9 ± 0.55 (++)	17.8 ± 0.23 (+++)

All values were means of three replicates, “+++” means transparent; “++” means clear; “+” means visible; “−” means no inhibitory effect.

Table 2. The MICs of Four Compounds against the Tested Bacteria.

Tested Bacteria	Minimum Inhibitory Concentration (μg·mL−1)
	7h	7h′	7e	7e′	Ampicillin
R. solanacearum	31.24	31.24	>125	>125	>125
P. syringae	7.81	7.81	>125	>125	>125
B. cereus	31.24	31.24	>125	>125	62.5
B. subtilis	3.91	3.91	>125	>125	>125

Table 2. The MICs of Four Compounds against the Tested Bacteria.

Tested Bacteria	Minimum Inhibitory Concentration (μg·mL−1)
	7h	7h′	7e	7e′	Ampicillin
R. solanacearum	31.24	31.24	>125	>125	>125
P. syringae	7.81	7.81	>125	>125	>125
B. cereus	31.24	31.24	>125	>125	62.5
B. subtilis	3.91	3.91	>125	>125	>125

2.3. Mechanism of Antibacterial Action

2.3.1. Fatty Acid Exposure Experiment

The results of the fatty acid exposure experiments was similar to those of yaglingmycin, that is the diameters of inhibition zone decrease significantly with the increase of the concentration of some fatty acids. It was worth nothing that compound 7h completely lost the antibacterial activity against B. subtilis in the presence of the long-chain saturated stearic acid, palmitic acid, and long-chain unsaturated oleic acid at the concentration of 500 ppm, 100 ppm, 1 ppm, respectively. Nevertheless, compound 7h did not lose the antibacterial activity against B. subtilis in the presence of short-chain n-hexylic acid and these results can be found in Figure 2.

Figure 2. The fatty acid exposure experiments of compound 7h.

Figure 2. The fatty acid exposure experiments of compound 7h.

2.3.2. Scanning Electron Microscope (SEM)

After treating B. cereus with 31.24 μg·mL⁻¹ 7h for 3 h, the morphological changes to the bacterial cells were observed by Scanning Electron Microscope (SEM). As shown in Figure 3, the surfaces of untreated bacterial cells were smooth and intact, while the morphology of treated cells changed dramatically. The treated cells had obvious depressions and deformations, some clear holes in the damaged cell walls and cell membranes and the cells became irregular, pitted, and shriveled. The morphological changes on the surface might result in diffusion of the contents from the cells to the outside.

Figure 3. Scanning Electron Microscope (SEM) photography of B. cereus. (A) Blank control; (B) Positive control (treated with yanglingmycin); (C) Treated with Compound 7h.

Figure 3. Scanning Electron Microscope (SEM) photography of B. cereus. (A) Blank control; (B) Positive control (treated with yanglingmycin); (C) Treated with Compound 7h.

3. Experimental Section

3.1. General Information

Solvents were of analytical reagent (AR) grade unless otherwise mentioned. TLC was performed on E. Merck 60 F254 silica gel plates. Column chromatography was carried out with 200~300 mesh silica gel (Qingdao Haiyang Co., Ltd., Qingdao, Shandong, China); compounds were eluted with the mixture solution of petroleum ether and ethyl acetate in sequence. Melting points (m.p.s, uncorrected) were measured using a Yanagimoto apparatus (Shanghai-Measuring Equipment Co., Ltd., Shanghai, China). ¹H-NMR (500 MHz) and ¹³C-NMR (125 MHz) were obtained on a Bruker-Avance-500 spectrometer (Bruker Corporation, Switzerland) with DMSO-d₆ or CDCl₃ as solvent and SiMe₄ as internal standard. Mass spectra were recorded under electrospray ionization (ESI) conditions by using a Thermo LCQ Fleet instrument (Thermo Fisher, Waltham, MA, USA).

3.2. Chemistry

3.2.1. Synthesis of 1e–10e and 1e′–10e′

Acetyl chloride (1.6 mL, 22 mmol) was slowly added to anhydrous MeOH (100 mL) at 0 °C. The solution was then stirred for 5 min, followed by addition of an amino acid (d-cysteine or l-cysteine) (20 mmol). After refluxing for 6 h, the reaction mixture was cooled to room temperature, and evaporated under reduced pressure to furnish the methyl d/l-cysteinate as a white solid in yields of 90% or 85%, respectively. To the anhydrous methanol solution (1.0 mL) of the benzonitriles (1.0 mmol) and anhydrous Na₂CO₃ (106 mg, 1.0 mmol) the above synthesized methyl cysteinate (5.0 mmol) was added, then the resulting mixture was stirred at 80 °C for 12 h, cooled to room temperature and concentrated. The crude mixture was purified by column chromatography on silica gel (PE/EA) to afford the desired 2-substituted-phenyl-4-methoxycarbonyl-4,5-dihydrothiazoles 1e–10e and 1e′–10e′ in 76% to 85% yields.

(S)-2-Phenyl-4-methoxycarbonyl-4,5-dihydrothiazole (1e). White powder. m.p. 60–62 °C. ¹H-NMR (CDCl₃) δ 7.87 (d, J = 7.6 Hz, 2H), 7.50–7.40 (m, 3H), 5.30 (t, J = 9.1 Hz, 1H), 3.84 (s, 3 H), 3.73 (dd, J = 8.8, 11.0 Hz, 1H), 3.67–3.62 (m, 1H). ¹³C-NMR (CDCl₃) δ 171.3, 171.0, 132.6, 131.7, 128.6 × 2, 128.5 × 2, 78.5, 52.7, 35.3. ESI-MS m/z: Calcd for C₁₁H₁₁NO₂S: 222.05 [M + H]⁺; found: 222.35. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +129.0° (c = 0.1, MeOH).

(S)-2-(4′-Hydroxyphenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (2e). White powder. m.p. 166–168 °C. ¹H-NMR (CDCl₃) δ 7.69 (d, J = 8.5 Hz, 2H), 6.76 (d, J = 8.5 Hz, 2H), 5.28 (t, J = 8.8 Hz, 1H), 3.79–3.77 (m, 3H), 3.72–3.62 (m, 2H). ¹³C-NMR (CDCl₃) δ 172.2, 171.5, 159.8, 130.6 × 2, 124.4, 115.6 × 2, 77.6, 52.8, 35.3. ESI-MS m/z: Calcd for C₁₁H₁₁NO₃S: 238.05 [M + H]⁺; found: 238.26. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +109.6° (c = 0.1, MeOH).

(S)-2-(4′-Fluorophenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (3e). Yellow oil. ¹H-NMR (CDCl₃) δ 7.89–7.86 (m, 2 H), 7.10 (t, J = 8.7 Hz, 2H), 5.28 (t, J = 9.1 Hz, 1H), 3.85 (s, 3H), 3.76–3.64 (m, 2H). ¹³C-NMR (CDCl₃) δ 171.2, 169.7, 164.8 (d, J = 252 Hz), 130.8, 130.7, 129.0, 115.7, 115.5, 78.4, 52.8, 35.7. ESI-MS m/z: Calcd for C₁₁H₁₀FNO₂S: 240.04 [M + H]⁺; found: 240.18. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +57.8° (c = 0.1, MeOH).

(S)-2-(4′-Bromophenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (4e). White solid. m.p. 74–76 °C. ¹H-NMR (CDCl₃) δ 7.74–7.73 (m, 2H), 7.56–7.54 (m, 2H), 5.27 (t, J = 9.1 Hz, 1H), 3.84 (s, 3H), 3.74 (dd, J = 8.8, 11.0 Hz, 1H), 3.66 (dd, J = 9.5, 11.0 Hz, 1H). ¹³C-NMR (CDCl₃) δ 171.1, 169.9, 131.7 × 2, 131.5, 130.0 × 2, 126.3, 78.5, 52.8, 35.6. ESI-MS m/z: Calcd for C₁₁H₁₀BrNO₂S: 299.96:301.96 = 1:1 [M + H]⁺; found: 299.69:301.85 = 1:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +7.6° (c = 0.1, MeOH).

(S)-2-(3′-Hydroxyphenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (5e). Colourless oil. ¹H-NMR (CDCl₃) δ 7.37 (s, 1H), 7.32 (d, J = 7.6 Hz, 1H), 7.22 (t, J = 8.0 Hz, 1H), 6.96 (dd, J = 1.9, 8.2 Hz, 1H), 5.29 (t, J = 9.1 Hz, 1H), 3.80 (s, 3H), 3.72–3.62 (m, 2H). ¹³C-NMR (CDCl₃) δ 172.2, 171.3, 156.2, 133.4, 129.8, 120.9, 119.4, 114.9, 77.9, 52.8, 35.2. ESI-MS m/z: Calcd for C₁₁H₁₁NO₃S: 238.05 [M + H]⁺; found: 238.22. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +50.2° (c = 0.1, MeOH).

(S)-2-(3′-Methylphenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (6e). Colourless oil. ¹H-NMR (CDCl₃) δ 7.72 (s, 1H), 7.64 (d, J = 4.1 Hz, 1H), 7.30 (d, J = 4.4 Hz, 2H), 5.29 (t, J = 9.1 Hz, 1H), 3.84 (s, 3H), 3.73–3.62 (m, 2H), 2.39 (s, 3H). ¹³C-NMR (CDCl₃) δ 171.4, 171.2, 138.3, 132.6, 132.5, 129.0, 128.4, 125.9, 78.4, 52.8, 35.3, 21.2. ESI-MS m/z: Calcd for C₁₂H₁₃NO₂S: 236.07 [M + H]⁺; found: 236.41. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +71.7° (c = 0.1, MeOH).

(S)-2-(2′-Hydroxyphenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (7e). Colorless oil. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +13.5° (c = 0.1, MeOH) [3].

(S)-2-(2′-Fluorophenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (8e). White solid. m.p. 70–72 °C. ¹H-NMR (CDCl₃) δ 7.95–7.92 (m, 1H), 7.47–7.43 (m, 1H), 7.21–7.11 (m, 2H), 5.26 (t, J = 9.5 Hz, 1H), 3.84 (s, 3H), 3.74–3.62 (m, 2H) ¹³C-NMR (CDCl₃) δ 171.2, 166.0 (d, J = 5.04 Hz), 160.6, (d, J = 255.78 Hz), 133.0 (d, J = 10.89 Hz), 130.7(d, J = 2.52 Hz), 124.1(d, J = 3.78 Hz), 120.7(d, J = 11.43 Hz), 116.3(d, J = 22.68 Hz), 52.8, 40.1, 35.4. ESI-MS m/z: Calcd for C₁₁H₁₀FNO₂S: 240.04 [M + H]⁺; found: 240.12. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +14.4° (c = 0.1, MeOH).

(S)-2-(2′-Chlorophenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (9e). Yellow oil. ¹H-NMR (CDCl₃) δ 7.66 (d, J = 7.6 Hz, 1H), 7.44–7.42 (m, 1H), 7.37–7.28 (m, 2H), 5.31 (t, J = 9.5 Hz, 1H), 3.85 (s, 3H), 3.79 (dd, J = 9.5, 11.0 Hz, 1H), 3.69 (t, J = 10.4 Hz, 1H). ¹³C-NMR (CDCl₃) δ 171.0, 169.0, 132.6, 132.3, 131.3, 130.7, 130.4, 126.7, 78.0, 52.8, 36.4. ESI-MS m/z: Calcd for C₁₁H₁₀ClNO₂S: 256.01:258.01 = 3:1 [M + H]⁺; found: 256.27:258.19 = 3:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +32.4° (c = 0.1, MeOH).

(S)-2-(2′-Bromophenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (10e). Yellow oil. ¹H-NMR (CDCl₃) δ 7.64–7.62 (m, 1H), 7.65–7.55 (m, 1H), 7.37–7.29 (m, 2H), 5.35–5.31 (m, 1 H), 3.86–3.84 (m, 3H), 3.81–3.69 (m, 2H). ¹³C-NMR (CDCl₃) δ 175.5, 170.9, 170.2, 134.5, 133.6, 131.4, 130.6, 127.2, 121.3, 78.3, 52.8, 36.5. ESI-MS m/z: Calcd for C₁₁H₁₀BrNO₂S: 299.96:301.96 = 1:1 [M + H]⁺; found: 299.88:301.76 = 1:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +22.0° (c = 0.1, MeOH).

(R)-2-Phenyl-4-methoxycarbonyl-4,5-dihydrothiazole (1e′). White powder. m.p. 60–62 °C. ¹H-NMR (CDCl₃) δ 7.87 (d, J = 7.3 Hz, 2H), 7.48 (t, J = 7.4 Hz, 1H), 7.41 (t, J = 7.6 Hz, 2H), 5.30 (t, J = 9.1 Hz, 1H), 3.84 (s, 3H), 3.73 (dd, J = 8.8, 11.0 Hz, 1H), 3.66–3.62 (m, 1H). ¹³C-NMR (CDCl₃) δ 171.3, 170.9, 132.6, 131.6, 128.6 × 2, 128.5 × 2, 78.5, 52.7, 35.3. ESI-MS m/z: Calcd for C₁₁H₁₁NO₂S: 222.05 [M + H]⁺; found: 222.24. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −129.0° (c = 0.1, MeOH).

(R)-2-(4′-Hydroxyphenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (2e′). White powder. m.p. 166–168 °C. ¹H-NMR (CDCl₃) δ 7.70–7.69 (m, 2H), 6.78–6.76 (m, 2H), 5.28 (t, J = 9.0 Hz, 1H), 3.79 (s, 3H), 3.72–3.62 (m, 2H). ¹³C-NMR (CDCl₃) δ 172.1, 171.5, 159.7, 130.6 × 2, 124.4, 115.5 × 2, 77.6, 52.8, 35.3. ESI-MS m/z: Calcd for C₁₁H₁₁NO₃S: 238.05 [M + H]⁺; found: 238.05. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −109.6° (c = 0.1, MeOH).

(R)-2-(4′-Fluorophenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (3e′). Yellow oil. ¹H-NMR (CDCl₃) δ 7.89–7.86 (m, 2H), 7.10 (t, J = 8.5 Hz, 2H), 5.28 (t, J = 9.1 Hz, 1H), 3.84 (s, 3H), 3.75–3.63 (m, 2H) ¹³C-NMR (CDCl₃) δ 171.2, 169.6, 164.8 (d, J = 252 Hz), 130.8, 130.7, 128.9, 115.7, 115.5, 78.4, 52.8, 35.6. ESI-MS m/z: Calcd for C₁₁H₁₀FNO₂S: 240.04 [M + H]⁺; found: 240.18. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −57.8° (c = 0.1, MeOH).

(R)-2-(4′-Bromophenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (4e′). White solid. m.p. 74–76 °C. ¹H-NMR (CDCl₃) δ 7.75–7.73 (m, J = 8.2 Hz, 2H), 7.57–7.55 (m, J = 8.2 Hz, 2H), 5.28 (t, J = 9.1 Hz, 1H), 3.85 (s, 3H), 3.76–3.72 (m, 1H), 3.68–3.64 (m, 1H). ¹³C-NMR (CDCl₃) δ 171.2, 170.0, 131.8 × 2, 131.6, 130.1 × 2, 126.4, 78.5, 52.9, 35.7. ESI-MS m/z: Calcd for C₁₁H₁₀BrNO₂S: 299.96:301.96 = 1:1 [M + H]⁺; found: 299.79:301.86 = 1:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −7.6° (c = 0.1, MeOH).

(R)-2-(3′-Hydroxyphenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (5e′). Colourless oil. ¹H-NMR (CDCl₃) δ 7.38 (s, 1H), 7.32 (d, J = 7.6 Hz, 1H), 7.23 (t, J = 7.9 Hz, 1H), 6.98–6.96 (m, 1H), 5.29 (t, J = 9.1 Hz, 1H), 3.81 (s, 3H), 3.72–3.62 (m, 2H). ¹³C-NMR (CDCl₃) δ 172.2, 171.3, 156.2, 133.5, 129.8, 121.0, 119.4, 114.8, 77.9, 52.9, 35.2. ESI-MS m/z: Calcd for C₁₁H₁₁NO₃S: 238.05 [M + H]⁺; found: 238.24. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −50.2° (c = 0.1, MeOH).

(R)-2-(3′-Methylphenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (6e′). Colourless oil. ¹H-NMR (CDCl₃) δ 7.72 (s, 1H), 7.65–7.64 (m, 1H), 7.30 (d, J = 4.7 Hz, 2H), 5.29 (t, J = 9.1 Hz, 1H), 3.84 (s, 3H), 3.74–3.61 (m, 2H), 2.39 (s, 3H). ¹³C-NMR (CDCl₃) δ 171.3, 171.2, 138.3, 132.5, 132.5, 128.9, 128.4, 125.9, 78.4, 52.7, 35.3, 21.2. ESI-MS m/z: Calcd for C₁₂H₁₃NO₂S: 236.07 [M + H]⁺; found: 236.16. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −71.7° (c = 0.1, MeOH).

(R)-2-(2′-Hydroxyphenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (7e′). Colorless oil. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −13.9° (c = 0.1, MeOH) [3].

(R)-2-(2′-Fluorophenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (8e′). White solid. m.p. 70–72 °C. ¹H-NMR (CDCl₃) δ 7.95–7.92 (m, 1H), 7.47–7.42 (m, 1H), 7.20–7.11 (m, 2H), 5.25 (t, J = 9.3 Hz, 1H), 3.84 (s, 3H), 3.74–3.62 (m, 2H). ¹³C-NMR (CDCl₃) δ 171.2, 165.9 (d, J = 5.04 Hz), 160.6 (d, J = 255.78 Hz), 133.0 (d, J = 8.82 Hz), 130.7 (d, J = 2.52 Hz), 124.1 (d, J = 2.52 Hz), 120.7 (d, J = 10.08 Hz), 116.3 (d, J = 22.68 Hz), 77.2, 52.8, 35.3 (d, J = 3.78 Hz). ESI-MS m/z: Calcd for C₁₁H₁₀FNO₂S: 240.04 [M + H]⁺; found: 240.25. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −14.4° (c = 0.1, MeOH).

(R)-2-(2′-Chlorophenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (9e′). Yellow oil. ¹H-NMR (CDCl₃) δ 7.65 (dd, J = 1.6, 7.6 Hz, 1H), 7.44–7.42 (m, 1H), 7.37–7.29 (m, 2H), 5.30 (t, J = 9.3 Hz, 1H), 3.85 (s, 3 H), 3.79 (dd, J = 9.1, 11.0 Hz, 1H), 3.69 (dd, J = 9.6, 11.2 Hz, 1H). ¹³C-NMR (CDCl₃) δ 171.0, 169.0, 132.6, 132.3, 131.3, 130.7, 130.4, 126.7, 78.0, 52.8, 36.3. ESI-MS m/z: Calcd for C₁₁H₁₀ClNO₂S: 256.01:258.01 = 3:1 [M + H]⁺; found: 256.12:258.37 = 3:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −32.4° (c = 0.1, MeOH).

(R)-2-(2′-Bromophenyl)-4-methoxycarbonyl-4,5-dihydrothiazole (10e′). Yellow oil. ¹H-NMR (CDCl₃) δ 7.64–7.54 (m, 2H), 7.37–7.24 (m, 2H), 5.35–5.29 (m, 1H), 3.85 (s, 3H), 3.83–3.67 (m, 2H). ¹³C-NMR (CDCl₃) δ 170.9, 170.1, 134.5, 133.6, 131.4, 130.6, 127.2, 121.3, 78.2, 52.8, 36.5. ESI-MS m/z: Calcd for C₁₁H₁₀BrNO₂S: 299.96:301.96 = 1:1 [M + H]⁺; found: 299.92:301.76 = 1:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −22.0° (c = 0.1, MeOH).

3.2.2. Synthesis of 1h–10h and 1h′–10h′

NaBH₄ (52.8 mg, 1.1 mmol) was added slowly in parts to a stirred solution of 1e–10e (1 mmol) in EtOH (10 mL). After 1 h, the reaction was complete (checked by TLC). Ethyl acetate was added, and then the mixture was washed with saturated aq. NaHCO₃ (2 × 5 mL), H₂O (2 × 5 mL), saturated aq. NaCl (2 × 5 mL), and dried with anhydrous Na₂SO₄. The mixture was concentrated under vacuum, and the crude product was chromatographed on silica gel to obtain the products 1e–10e and 1e′–10e′. The yields of 1h–10h and 1h′–10h′ were from 76% to 89%.

(S)-2-Phenyl-4-hydroxymethyly-4,5-dihydrothiazole (1h). Yellow solid. m.p. 76–78 °C. ¹H-NMR (CDCl₃) δ 7.81 (d, J = 7.6 Hz, 2H), 7.48–7.39 (m, 3H), 4.83–4.77 (m, 1H), 4.02 (dd, J = 4.6, 11.2 Hz, 1H), 3.80 (dd, J = 5.7, 11.3 Hz, 1H), 3.46–3.42 (m, 1H), 3.33–3.29 (m, 1H). ¹³C-NMR (CDCl₃) δ 169.7, 132.9, 131.4, 128.5 × 2, 128.4 × 2, 79.3, 64.5, 34.4. ESI-MS m/z: Calcd for C₁₀H₁₁NOS: 194.06 [M + H]⁺; found: 194.22. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +30.7° (c = 0.1, MeOH).

(S)-2-(4′-Hydroxyphenyl)-4-hydroxymethyl-4,5-dihydrothiazole (2h). White solid. m.p. 196–198 °C. ¹H-NMR (DMSO-d₆) δ 10.06 (s, 1H), 7.59 (d, J = 8.5 Hz, 2H), 6.81 (d, J = 8.5 Hz, 2H), 4.91 (t, J = 5.4 Hz, 1H), 4.66–4.60 (m, 1H), 3.68–3.64 (m, 1H), 3.50–3.40 (m, 2H), 3.30–3.27 (m, 1H). ¹³C-NMR (DMSO-d₆) δ 165.3, 160.2, 129.9 × 2, 124.0, 115.3 × 2, 79.3, 62.3, 34.5. ESI-MS m/z: Calcd for C₁₀H₁₁NO₂S: 210.05 [M + H]⁺; found: 210.23. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +124.0° (c = 0.1, MeOH).

(S)-2-(4′-Fluorophenyl)-4-hydroxymethyly-4,5-dihydrothiazole (3h). White solid. m.p. 60–62 °C. ¹H-NMR (CDCl₃) δ 7.79–7.76 (m, 2H), 7.07 (t, J = 10 Hz, 2H), 4.79–4.74 (m, 1H), 4.03 (dd, J = 4.4, 11.3 Hz, 1H), 3.78 (dd, J = 5.5, 11.2 Hz, 1H), 3.43 (dd, J = 9.0, 10.9 Hz, 1H), 3.35–3.31 (m, 1H). ¹³C-NMR (CDCl₃) δ 168.4, 164.6 (d, J = 252 Hz) 130.5, 130.4, 129.1, 115.6, 115.4, 79.3, 64.3, 34.6. ESI-MS m/z: Calcd for C₁₀H₁₀FNOS: 212.05 [M + H]⁺; found: 212.31. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +10.9° (c = 0.1, MeOH).

(S)-2-(4′-Bromophenyl)-4-hydroxymethyl-4,5-dihydrothiazole (4h). White solid. m.p. 96–98 °C. ¹H-NMR (CDCl₃) δ 7.67–7.65 (m, 2H), 7.54–7.52 (m, 2H), 4.80–4.75 (m, 1H), 4.03 (dd, J = 4.7, 11.3 Hz, 1H), 3.79 (dd, J = 5.7, 11.3 Hz, 1H), 3.46 (dd, J = 8.8, 10.7 Hz, 1H), 3.33 (dd, J = 9.3, 10.9 Hz, 1H). ¹³C-NMR (CDCl₃) δ 168.7, 131.8, 131.7 × 2, 129.8 × 2, 126.0, 79.4, 64.4, 34.6. ESI-MS m/z: Calcd for C₁₀H₁₀BrNOS: 271.97:273.97 = 1:1 [M + H]⁺; found: 272.16:274.04 = 1:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +26.6° (c = 0.1, MeOH).

(S)-2-(3′-Hydroxyphenyl)-4-hydroxymethyl-4,5-dihydrothiazole (5h). White solid. m.p. 118–120 °C. ¹H-NMR (CD₃OD) δ 7.27–7.23 (m, 3H), 6.98–6.96(m, 1H), 4.78–4.73 (m, 1H), 4.04 (s, 2H), 3.85–3.78 (m, 2H). ¹³C-NMR (CD₃OD) δ 171.1, 156.8, 133.6, 129.5, 119.7, 118.7, 114.2, 78.5, 63.1, 34.2. ESI-MS m/z: Calcd for C₁₀H₁₁NO₂S: 210.05 [M + H]⁺; found: 210.27. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +178.2° (c = 0.1, MeOH).

(S)-2-(3′-Methylphenyl)-4-hydroxymethyl-4,5-dihydrothiazole (6h). Yellow oil. ¹H-NMR (CDCl₃) δ 7.62–7.57 (m, 2H), 7.28–7.27 (m, 2H), 4.81–4.75 (m, 1H), 4.02 (dd, J = 4.7, 11.0 Hz, 1H), 3.79 (dd, J = 5.5, 11.2 Hz, 1H), 3.42 (dd, J = 8.8, 10.7 Hz, 1H), 3.31 (dd, J = 9.5, 10.7 Hz, 1H), 2.38 (s, 3H). ¹³C-NMR (CDCl₃) δ 169.9, 138.2, 132.8, 132.1, 128.8, 128.3, 125.6, 79.3, 64.4, 34.3, 21.2. ESI-MS m/z: Calcd for C₁₁H₁₃NOS: 208.07 [M + H]⁺; found: 208.32. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +7.6° (c = 0.1, MeOH).

(S)-2-(2′-Hydroxyphenyl)-4-hydroxymethyl-4,5-dihydrothiazole (7h). White solid. m.p. 43–45 °C. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +16.5° (c = 0.1, MeOH) [3].

(S)-2-(2′-Fluorophenyl)-4-hydroxymethyl-4,5-dihydrothiazole (8h). White solid. m.p. 78–80 °C. ¹H-NMR (CDCl₃) δ 7.86–7.82 (m, 1H), 7.46–7.41 (m, 1H), 7.20–7.11 (m, 2H), 4.81–4.75 (m, 1H), 4.00 (dd, J = 4.9, 11.2 Hz, 1H), 3.80 (dd, J = 5.7, 11.3 Hz, 1H), 3.44 (dd, J = 9.1, 10.7 Hz, 1H), 3.30 (dd, J = 9.1, 10.7 Hz, 1H). ¹³C-NMR (CDCl₃) δ 164.6 (d, J = 5.04 Hz), 160.3 (d, J = 255.78 Hz), 132.6 (d, J = 8.82 Hz), 130.5 (d, J = 1.26 Hz), 124.1 (d, J = 3.78 Hz), 121.1 (d, J = 10.1 Hz), 116.4 (d, J = 25.2 Hz), 78.4, 64.4, 34.4. ESI-MS m/z: Calcd for C₁₀H₁₀FNOS: 212.05 [M + H]⁺; found: 212.41. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +25.9° (c = 0.1, MeOH).

(S)-2-(2′-Chlorophenyl)-4-hydroxymethyl-4,5-dihydrothiazole (9h). Yellow oil. ¹H-NMR (CDCl₃) δ 7.57 (dd, J = 1.6, 7.6 Hz, 1H), 7.44–7.42 (m, 1H), 7.36–7.29 (m, 2H), 4.85–4.80 (m, 1H), 3.95 (dd, J = 4.9, 11.2 Hz, 1H), 3.78 (dd, J = 5.5, 11.2 Hz, 1H), 3.48 (dd, J = 9.1, 10.7 Hz, 1H), 3.36 (dd, J = 8.7, 10.9 Hz, 1H). ¹³C-NMR (CDCl₃) δ 167.5, 132.7, 132.1, 131.1, 130.4, 130.3, 126.7, 79.2, 64.2, 35.5. ESI-MS m/z: Calcd for C₁₀H₁₀ClNOS: 228.02:230.02 = 3:1 [M + H]⁺; found: 228.21:230.37 = 3:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +19.4° (c = 0.1, MeOH).

(S)-2-(2′-Bromophenyl)-4-hydroxymethyl-4,5-dihydrothiazole (10h). Yellow solid. m.p. 54–56 °C. ¹H-NMR (CDCl₃) δ 7.64 (d, J = 7.9 Hz, 1H), 7.51 (d, J = 7.6 Hz, 1H), 7.37–7.29 (m, 2H), 4.91–4.86 (m, 1H), 4.04–4.01 (m, 1H), 3.81 (dd, J = 5.5, 11.2 Hz, 1H), 3.55–3.51 (m, 1H), 3.42 (dd, J = 8.8, 10.7 Hz, 1H). ¹³C-NMR (CDCl₃) δ 168.7, 134.8, 133.6, 131.2, 130.2, 127.3, 121.0, 79.5, 64.5, 35.7. ESI-MS m/z: Calcd for C₁₀H₁₀BrNOS 271.97:273.97 = 1:1 [M + H]⁺; found: 271.86:273.91 = 1:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +109.1° (c = 0.1, MeOH).

(R)-2-Phenyl-4-hydroxymethyl-4,5-dihydrothiazole (1h′). Yellow solid. m.p. 76–78 °C. ¹H-NMR (CDCl₃) δ 7.79 (d, J = 7.9 Hz, 2H), 7.47–7.37 (m, 4H), 4.80–4.75 (m, 1H), 4.01 (dd, J = 4.7, 11.0 Hz, 1H), 3.79 (dd, J = 5.5, 11.2 Hz, 1H), 3.44–3.40 (m, 1H), 3.33–3.29 (m, 1H), 2.78 (br. s, 1H). ¹³C-NMR (CDCl₃) δ 169.8, 132.8, 131.4, 128.4 × 2, 128.3 × 2, 79.3, 64.3, 34.3. ESI-MS m/z: Calcd for C₁₀H₁₁NO₂S: 194.06 [M + H]⁺; found: 194.23. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −30.6° (c = 0.1, MeOH).

(R)-2-(4′-Hydroxyphenyl)-4-hydroxymethyl-4,5-dihydrothiazole (2h′). White solid. m.p. 196–198 °C. ¹H-NMR (DMSO-d₆) δ 10.12 (s, 1H), 7.75–7.60 (m, 2H), 6.95–6.80 (m, 2H), 4.97 (t, J = 5.7 Hz, 1H), 4.72–4.66 (m, 1H), 3.75–3.70 (m, 1H), 3.56–3.51 (m, 1H), 3.48 (dd, J = 8.7, 10.9 Hz, 1H), 3.35 (dd, J = 7.4, 10.9 Hz, 1H). ¹³C-NMR (DMSO-d₆) δ 165.3, 160.2, 129.8 × 2, 124.0, 115.3 × 2, 79.3, 62.3, 34.5. ESI-MS m/z: Calcd for C₁₁H₁₁NO₂S: 210.05 [M + H]⁺; found: 210.16. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −123.9° (c = 0.1, MeOH).

(R)-2-(4′-Fluorophenyl)-4-hydroxymethyl-4,5-dihydrothiazole (3h′). White solid. m.p. 60–62 °C. ¹H-NMR (CDCl₃) δ 7.81 (dd, J = 5.4, 8.8 Hz, 2H), 7.08 (t, J = 8.5 Hz, 2H), 4.81–4.75 (m, 1H), 4.02 (dd, J = 4.6, 11.2 Hz, 1H), 3.79 (dd, J = 5.7, 11.0 Hz, 1H), 3.45 (dd, J = 8.8, 10.7 Hz, 1H), 3.34–3.30 (m, 1H). ¹³C-NMR (CDCl₃) δ 168.3, 164.6 (d, J = 252 Hz), 130.5, 130.5, 129.2, 115.6, 115.5, 79.3, 64.5, 34.7. ESI-MS m/z: Calcd for C₁₀H₁₀FNOS: 212.05 [M + H]⁺; found: 212.11. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −10.9° (c = 0.1, MeOH).

(R)-2-(4′-Bromophenyl)-4-hydroxymethyl-4,5-dihydrothiazole (4h′). White solid. m.p. 96–98 °C. ¹H-NMR (CDCl₃) δ 7.68–7.66 (m, 2H), 7.55–7.53 (m, 2H), 4.81–4.76 (m, 1H), 4.03 (dd, J = 4.7, 11.0 Hz, 1H), 3.79 (dd, J = 5.7, 11.3 Hz, 1H), 3.46 (dd, J = 8.8, 10.7 Hz, 1H), 3.33 (dd, J = 9.3, 10.9 Hz, 1H). ¹³C-NMR (CDCl₃) δ 168.5, 131.8, 131.7 × 2, 129.8 × 2, 126.0, 79.4, 64.5, 34.6. ESI-MS m/z: Calcd for C₁₀H₁₀BrNOS: 271.97:273.97 = 1:1 [M + H]⁺; found: 271.82:273.77 = 1:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −26.6° (c = 0.1, MeOH).

(R)-2-(3′-Hydroxyphenyl)-4-hydroxymethyl-4,5-dihydrothiazole (5h′). White solid. m.p. 118–120 °C. ¹H-NMR (CDCl₃) δ 7.35 (br. s, 1H), 7.27–7.24 (m, 2H), 6.97–6.96 (m, 1H), 4.75–4.74 (m, 1H), 3.82–3.80 (m, 2H), 3.62–3.61 (m, 2H). ¹³C-NMR (CDCl₃) δ 171.3, 156.8, 133.6, 129.6, 120.0, 118.9, 114.0, 78.5, 63.3, 34.2. ESI-MS m/z: Calcd for C₁₀H₁₁NO₂S: 210.05 [M + H]⁺; found: 210.23. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −178.2° (c = 0.1, MeOH).

(R)-2-(3′-Methylphenyl)-4-hydroxymethyl-4,5-dihydrothiazole (6h′). Yellow oil. ¹H-NMR (CDCl₃) δ 7.61–7.56 (m, 2H), 7.29–7.26 (m, 2H), 4.79–4.74 (m, 1H), 4.01 (dd, J = 4.7, 11.3 Hz, 1H), 3.78 (dd, J = 5.5, 11.2 Hz, 1H), 3.41 (dd, J = 8.8, 10.7 Hz, 1H), 3.30 (dd, J = 9.1, 10.7 Hz, 1H), 2.37 (s, 3H). ¹³C-NMR (CDCl₃) δ 169.9, 138.2, 132.7, 132.1, 128.8, 128.3, 125.6, 79.2, 64.3, 34.2, 21.2. ESI-MS m/z: Calcd for C₁₁H₁₃NOS: 208.07 [M + H]⁺; found: 208.01. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −7.6° (c = 0.1, MeOH).

(R)-2-(2′-Hydroxyphenyl)-4-hydroxymethyl-4,5-dihydrothiazole (7h′). White solid. m.p. 44–46 °C. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −16.1° (c = 0.1, MeOH) [3].

(R)-2-(2′-Fluorophenyl)-4-hydroxymethyl-4,5-dihydrothiazole (8h′). White solid. m.p. 78–80 °C. ¹H-NMR (CDCl₃) δ 7.94–7.75 (m, 1H), 7.57–7.34 (m, 1H), 7.24–7.02 (m, 2H), 4.86–4.71 (m, 1H), 4.00 (dd, J = 4.7, 11.3 Hz, 1H), 3.80 (dd, J = 5.7, 11.0 Hz, 1H), 3.44 (dd, J = 9.0, 10.9 Hz, 1H), 3.31 (dd, J = 9.5, 10.7 Hz, 1H), 2.62 (br. s, 1H). ¹³C-NMR (CDCl₃) δ 164.6 (d, J = 5.04 Hz), 160.4, (d, J = 255.78 Hz), 132.6 (d, J = 8.82 Hz), 130.5 (d, J = 2.52 Hz), 124.1 (d, J = 3.78 Hz), 121.1 (d, J = 11.34 Hz), 116.4 (d, J = 22.68 Hz), 78.4, 64.4, 34.4 (d, J = 2.52 Hz). ESI-MS m/z: Calcd for C₁₀H₁₀FNOS: 212.05 [M + H]⁺; found: 212.29. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −25.9° (c = 0.1, MeOH).

(R)-2-(2′-Chlorophenyl)-4-hydroxymethyl-4,5-dihydrothiazole (9h′). Yellow oil. ¹H-NMR (CDCl₃) δ 7.58 (d, J = 7.6 Hz, 1H), 7.44 (d, J = 7.6 Hz, 1H), 7.36–7.28 (m, 2H), 4.86–4.80 (m, 1H), 3.96 (dd, J = 4.9, 11.2 Hz, 1H), 3.81–3.77 (m, 1H), 3.53–3.47 (m, 1H), 3.41–3.35 (m, 1H). ¹³C-NMR (CDCl₃) δ 167.5, 132.7, 132.1, 131.1, 130.4, 130.4, 126.7, 79.2, 64.2, 35.5. ESI-MS m/z: Calcd for C₁₀H₁₀ClNOS: 228.02:230.02 = 3:1 [M + H]⁺; found: 228.16:230.27 = 3:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −19.4° (c = 0.1, MeOH).

(R)-2-(2′-Bromophenyl)-4-hydroxymethyl-4,5-dihydrothiazole (10h′). Yellow solid. m.p. 54–56 °C. ¹H-NMR (CDCl₃) δ 7.69–7.63 (m, 1H), 7.55–7.50 (m, 1H), 7.36–7.28 (m, 1H), 4.90–4.85 (m, 1H), 4.03–3.95 (m, 1H), 3.83–3.76 (m, 1H), 3.55–3.51 (m, 1H), 3.46–3.39 (m, 1H). ¹³C-NMR (CDCl₃) δ 168.7, 133.6, 131.7, 131.2, 130.2, 129.8, 127.3, 79.4, 64.4, 35.6. ESI-MS m/z: Calcd for C₁₀H₁₀BrNOS: 271.97:273.97 = 1:1 [M + H]⁺; found: 271.82:273.79 = 1:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −109.1° (c = 0.1, MeOH).

3.2.3. Synthesis of 1a–10a and 1a′–10a′

10% sodium hydroxide solution was added slowly in parts to a stirred solution of 1e–10e and 1e′–10e′ (1mmol) in EtOH (10 mL). After 2 h, the reaction was complete (checked by TLC). Purification of the crude reaction mixture by column chromatography on silica gel (PE/ EA) afforded the desired products and the yields were from 81% to 90%.

(S)-2-Phenyl-4-carboxy-4,5-dihydrothiazole (1a). Yellow powder. m.p. 116–118 °C. ¹H-NMR (CDCl₃) δ 7.83 (d, J = 7.6 Hz, 2H), 7.48–7.45 (m, 1H), 7.40–7.38 (m, 2H), 5.26 (t, J = 9.1 Hz, 1H), 3.72–3.64 (m, 2H). ¹³C-NMR (CDCl₃) δ 172.6, 171.8, 132.4, 131.7, 128.9 × 2, 128.1 × 2, 78.1, 35.1. ESI-MS m/z: Calcd for C₁₀H₉NO₂S: 208.04 [M + H]⁺; found: 208.25. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +10.5° (c = 0.1, MeOH).

(S)-2-(4′-Hydroxyphenyl)-4-carboxy-4,5-dihydrothiazole (2a). White solid. m.p. 150–152 °C. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +12.5° (c = 0.1, MeOH) [13].

(S)-2-(4′-Fluorophenyl)-4-carboxy-4,5-dihydrothiazole (3a). White solid. m.p. 120–122 °C. ¹H-NMR (CDCl₃) δ 9.14 (br. s, 1 H), 7.87 (dd, J = 5.4, 8.5 Hz, 2H), 7.12 (t, J = 8.5 Hz, 2H), 5.38 (t, J = 9.1 Hz, 1H), 3.78–3.70 (m, 2H). ¹³C-NMR (CDCl₃) δ 173.6, 172.0, 165.1 (d, J = 252 Hz), 130.9, 130.9, 128.3, 115.9, 115.7, 77.6, 35.3. ESI-MS m/z: Calcd for C₁₀H₈FNO₂S: 226.03 [M + H]⁺; found: 226.08. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +25.3° (c = 0.1, MeOH).

(S)-2-(4′-Bromophenyl)-4-carboxy-4,5-dihydrothiazole (4a). White power. m.p. 194–196 °C. ¹H-NMR (CDCl₃) δ 7.74–7.72 (m, 2H), 7.59–7.58 (m, 2H), 5.35 (t, J = 9.6 Hz, 1H), 3.76 (d, J = 9.8 Hz, 2H). ¹³C-NMR (CDCl₃) δ 172.5, 171.9, 132.0 × 2, 130.9, 130.0 × 2, 127.0, 78.0, 35.2. ESI-MS m/z: Calcd for C₁₀H₈BrNO₂S: 285.95:287.95 = 1:1 [M + H]⁺; found: 285.91:287.75 = 1:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +14.6° (c = 0.1, MeOH).

(S)-2-(3′-Hydroxyphenyl)-4-carboxy-4,5-dihydrothiazole (5a). Yellow oil. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +13.3° (c = 0.1, MeOH) [14].

(S)-2-(3′-Methylphenyl)-4-carboxy-4,5-dihydrothiazole (6a). Yellow power. m.p. 118–120 °C. ¹H-NMR (CDCl₃) δ 7.66 (br. s, 1H), 7.64–7.49 (m, 1H), 7.48–7.27 (m, 2H), 5.25 (t, J = 9.1 Hz, 1H), 3.70–3.61 (m, 2H), 2.36 (s, 3H). ¹³C-NMR (CDCl₃) δ 172.6, 172.0, 138.2, 132.5, 132.2, 128.8, 128.3, 125.6, 78.0, 35.0, 21.0. ESI-MS m/z: Calcd for C₁₁H₁₁NO₂S: 222.05 [M + H]⁺; found: 222.33. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +30.2° (c = 0.1, MeOH).

(S)-2-(2′-Hydroxyphenyl)-4-carboxy-4,5-dihydrothiazole (7a). Yellow solid. m.p. 128–130 °C. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +159.9° (c = 0.1, MeOH) [15].

(S)-2-(2′-Fluorophenyl)-4-carboxy-4,5-dihydrothiazole (8a). Yellow oil. ¹H-NMR (DMSO-d₆) δ 7.90–7.87 (m, 1H), 7.69–7.57 (m, 2H), 7.31–7.27 (m, 1H), 5.26 (t, J = 9.0 Hz, 1H), 3.73–3.68 (m, 1H), 3.63–3.59 (m, 1H). ¹³C-NMR (DMSO-d₆) δ 171.7, 171.4, 163.1(d, J = 3.78 Hz), 133.6(d, J = 7.56 Hz), 130.3(d, J = 1.26 Hz), 124.9 (d, J = 2.52 Hz), 120.2(d, J = 7.56 Hz), 116.6(d, J = 8.82 Hz), 77.2, 35.0(d, J = 5.04 Hz). ESI-MS m/z: Calcd for C₁₁H₁₁NO₂S: 226.03 [M + H]⁺; found: 226.03. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +96.8° (c = 0.1, MeOH).

(S)-2-(2′-Chlorophenyl)-4-carboxy-4,5-dihydrothiazole (9a). Yellow solid. m.p. 120–122 °C. ¹H-NMR (CDCl₃) δ 7.63 (d, J = 7.6 Hz, 1H), 7.46–7.43 (m, 1H), 7.39 (d, J = 7.9 Hz, 1H), 7.32–7.28 (m, 1H), 5.38 (t, J = 9.5 Hz, 1H), 3.92–3.68 (m, 2H). ¹³C-NMR (CDCl₃) δ 173.1, 171.4, 132.5, 131.8, 131.5, 130.8, 130.6, 126.9, 77.5, 36.0. ESI-MS m/z: Calcd for C₁₀H₈ClNO₂S: 242.00:244.00 = 3:1 [M + H]⁺; found: 242.36:244.48 = 3:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +62.6° (c = 0.1, MeOH).

(S)-2-(2′-Bromophenyl)-4-carboxy-4,5-dihydrothiazole (10a). Yellow oil. ¹H-NMR (CDCl₃) δ 7.65 (d, J = 8.2 Hz, 1H), 7.59–7.54 (m, 1H), 7.38–7.22 (m, 2H), 5.41 (t, J = 9.5 Hz, 1H), 3.87–3.76 (m, 2H). ¹³C-NMR (CDCl₃) δ 172.6, 167.5, 133.9, 131.9, 130.6, 129.7, 127.4, 121.2, 77.8, 36.1. ESI-MS m/z: Calcd for C₁₀H₈BrNO₂S: 285.95:287.95 = 1:1 [M + H]⁺; found: 285.87:287.92 = 1:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ +74.5° (c = 0.1, MeOH).

(R)-2-Phenyl-4-carboxy-4,5-dihydrothiazole (1a′). Yellow powder. m.p. 116–118 °C. ¹H-NMR (CDCl₃) δ 7.83 (d, J = 7.6 Hz, 2H), 7.48–7.45 (m, 1H), 7.40–7.38 (m, 2H), 5.26 (t, J = 9.1 Hz, 1H), 3.72–3.64 (m, 2H). ¹³C-NMR (CDCl₃) δ 172.6, 171.8, 132.4, 131.7, 128.9 × 2, 128.1 × 2, 78.1, 35.1. ESI-MS m/z: Calcd for C₁₀H₉NO₂S: 208.04 [M + H]⁺; found: 208.26. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −10.5° (c = 0.1, MeOH).

(R)-2-(4′-Hydroxyphenyl)-4-carboxy-4,5-dihydrothiazole (2a′). White solid. m.p. 150–152 °C. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −12.5° (c = 0.1, MeOH) [13].

(R)-2-(4′-Fluorophenyl)-4-carboxy-4,5-dihydrothiazole (3a′). White solid. m.p. 120–122 °C. ¹H-NMR (CDCl₃) δ 10.23 (br. s, 1H), 7.86 (dd, J = 5.4, 8.5 Hz, 2H), 7.13–7.09 (m, 2H), 5.41–5.37 (m, 1H), 3.78–3.69 (m, 2H). ¹³C-NMR (CDCl₃) δ 173.7, 172.0, 165.1 (d, J = 252 Hz), 130.9, 130.9, 128.3, 115.9, 115.7, 77.5, 35.3. ESI-MS m/z: Calcd for C₁₀H₈FNO₂S: 226.04 [M + H]⁺; found: 226.33. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −25.3° (c = 0.1, MeOH).

(R)-2-(4′-Bromophenyl)-4-carboxy-4,5-dihydrothiazole (4a′). White power. m.p. 194–196 °C. ¹H-NMR (DMSO-d₆) δ 7.85–7.66 (m, 4H), 5.31 (t, J = 8.8 Hz, 1H), 3.84–3.70 (m, 1H), 3.70–3.59 (m, 1H). ¹³C-NMR (DMSO-d₆) δ 171.6, 167.3, 131.9 × 2, 131.4, 130.0 × 2, 125.4, 78.4, 35.2. ESI-MS m/z: Calcd for C₁₀H₈BrNO₂S: 285.95:287.95 = 1:1 [M + H]⁺; found: 285.92:287.86 = 1:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −14.6° (c = 0.1, MeOH).

(R)-2-(3′-Hydroxyphenyl)-4-carboxy-4,5-dihydrothiazole (5a′). Yellow oil. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −13.3° (c = 0.1, MeOH) [14].

(R)-2-(3′-Methylphenyl)-4-carboxy-4,5-dihydrothiazole (6a′). Yellow power. m.p. 118–120 °C. ¹H-NMR (CDCl₃) δ 7.67 (br. s, 1H), 7.64–7.54 (m, 1H), 7.40–7.27 (m, 2H), 5.25 (t, J = 9.1 Hz, 1H), 3.77–3.56 (m, 2H), 2.36 (s, 3H). ¹³C-NMR (CDCl₃) δ 172.6, 172.0, 138.2, 132.5, 132.3, 128.9, 128.3, 125.7, 78.0, 35.0, 21.0. ESI-MS m/z: Calcd for C₁₁H₁₁NO₂S: 222.05 [M + H]⁺; found: 222.27. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −30.2° (c = 0.1, MeOH).

(R)-2-(2′-Hydroxyphenyl)-4-carboxy-4,5-dihydrothiazole (7a′). Yellow solid. m.p. 128–130 °C. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −160.0° (c = 0.1, MeOH) [15].

(R)-2-(2′-Fluorophenyl)-4-carboxy-4,5-dihydrothiazole (8a′). Yellow oil. ¹H-NMR (DMSO-d₆) δ 7.90–7.87 (m, 1H), 7.69–7.57 (m, 2H), 7.31–7.27 (m, 1H), 5.26 (t, J = 9.0 Hz, 1H), 3.73–3.68 (m, 1H), 3.63–3.59 (m, 1H). ¹³C-NMR (DMSO-d₆) δ 171.7, 171.4, 163.1 (d, J = 3.78 Hz), 133.6 (d, J = 7.56 Hz), 130.3(d, J = 1.26 Hz), 124.9 (d, J = 2.52 Hz), 120.2 (d, J = 7.56 Hz), 116.6 (d, J = 8.82 Hz), 77.2, 35.0 (d, J = 5.04 Hz). ESI-MS m/z: Calcd for C₁₀H₈FNO₂S: 226.03 [M + H]⁺; found: 226.18. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −96.8° (c = 0.1, MeOH).

(R)-2-(2′-Chlorophenyl)-4-carboxy-4,5-dihydrothiazole (9a′). Yellow solid. m.p. 120–122 °C. ¹H-NMR (CDCl₃) δ 7.63 (d, J = 7.6 Hz, 1H), 7.46–7.43 (m, 1H), 7.39 (d, J = 7.9 Hz, 1H), 7.32–7.28 (m, 1H), 5.38 (t, J = 9.5 Hz, 1H), 3.92–3.68 (m, 2H). ¹³C-NMR (CDCl₃) δ 173.1, 171.4, 132.5, 131.8, 131.5, 130.8, 130.6, 126.9, 77.5, 36.0. ESI-MS m/z: Calcd for C₁₀H₈ClNO₂S: 242.00:244.00 = 3:1 [M + H]⁺; found: 242.06:244.12 = 3:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −62.6° (c = 0.1, MeOH).

(R)-2-(2′-bromophenyl)-4-carboxy-4,5-dihydrothiazole (10a′). Yellow oil. ¹H-NMR (CDCl₃) δ 7.65 (d, J = 8.2 Hz, 1H), 7.59–7.54 (m, 1H), 7.38–7.22 (m, 2H), 5.41 (t, J = 9.5 Hz, 1H), 3.87–3.76 (m, 2H). ¹³C-NMR (CDCl₃) δ 172.6, 167.5, 133.9, 131.9, 130.6, 129.7, 127.4, 121.2, 77.8, 36.1. ESI-MS m/z: Calcd for C₁₀H₈BrNO₂S: 285.95:287.95 = 1:1 [M + H]⁺; found: 285.87:287.69 = 1:1. ${\left[\mathrm{\alpha }\right]}_{\text{D}}^{20}$ −74.5° (c = 0.1, MeOH).

3.3. Biology

3.3.1. Filter Paper Assay

The standard bacterial stains were provided by the Institute of Plant Disease, Northwest Agriculture and Forestry University. Ampicillin (Sigma, Shanghai, China) was preferred as the positive control. Mueller–Hinton (Hangzhou Microbial Reagent Co., Ltd., Hangzhou, China) agar was used as an assay medium. The medium at 45 °C was mixed with a suspension containing the bacterial pathogen at approximately 108 colony forming units (CFU)·mL⁻¹. Petri dishes (9 cm in diameter) were then flooded with the mixture. The tested samples were dissolved in acetone at the concentration of 1000 ppm, the filter papers (6 mm in diameter) were impregnated with 10 μL/disc of each compound, then were completely dried and placed on the inoculated agar. The inoculated plates were incubated at 37 °C for 10–12 h. Antibacterial activity was evaluated by measuring the zone of inhibition against the test organism. Experiments were run in triplicate.

3.3.2. Minimal Inhibitory Concentration (MIC)

Antibacterial activities were measured by the micro-broth dilution method in 96-well culture plates using the Mueller–Hinton broth, according to the National Committee for Clinical Laboratory Standards. The tested bacteria were incubated in the Mueller–Hinton broth for 12 h at 37 °C at 190 rpm, and the spore concentration was diluted to approximately 1 × 10⁵–1 × 10⁶ CFU/mL with Mueller–Hinton broth. After incubation for 24 h at 37 °C, the MICs were examined by observing the first few holes transparent [3].

3.3.3. The Fatty Acid Exposure Experiment

The medium at 45 °C was mixed with a suspension containing the bacterial pathogen at approximately 108 CFU·mL⁻¹ and fatty acid or fatty acid salt of certain concentration gradient. Petri dishes (9 cm in diameter) were then flooded with the mixture. Compound 7h was dissolved in acetone at the concentration of 100 ppm, the filter papers (6 mm in diameter) were impregnated with 10 μL/disc of each compound. The inoculated plates were incubated at 37 °C for 10–12 h. The fatty acid added experiment was evaluated by measuring the zone of inhibition against the test organism. The experiments were run in triplicate.

3.3.4. Scanning Electron Microscopy

The tested bacteria were incubated in the Mueller–Hinton broth for 12 h at 37 °C at 180 rpm and the spore concentration was diluted to approximately 1 × 10⁵–1 × 10⁶ CFU/mL with Mueller–Hinton broth. Compound 7h was dissolved in acetone and added to the broth. After incubation for 2 h at 37 °C then centrifuged at 3500 rpm for 20 min. All the cells were washed twice with 0.1 M phosphate buffer saline (PBS, pH 7.4) and fixed with 2.5% glutaraldehyde (v/v) in 0.1 M PBS at 4 °C for 24 h. Next, the cells were dehydrated using 30%, 50%, 70%, 90%, and 100% ethanol, and then the ethanol was replaced by tertiary butyl alcohol. The cells were dried at “critical point” in liquid CO₂, and the samples were gold-covered by cathodic spraying before examination [16,17].

4. Conclusions

Aryl nitriles and methyl cysteine reacted in the presence of sodium carbonate in dry methanol to afford methyl-2-aryl-4, 5-dihydrothiazole-4-carboxylates in high yield. Ortho substituents did not favor the reaction, especially some large groups, such as -Br and -Cl. Meanwhile electron-withdrawing groups were better to this reaction than electron-donating ones.

Not only electron-withdrawing but also electron-donating groups could diminish the antibacterial activities unless a 2′-hydroxy was present in the 2-aryl substituent of the 4,5-dihydro-thiazole analogues. These results implied that the intramolecular hydrogen bond between the 2′-hydroxy and the nitrogen-atom of 4,5-dihydrothiazole is very important for the antibacterial activities of these compounds.

According to the fatty acid exposure experiments at high concentrations of fatty acid (1000 ppm), when short chain acids were added in culture medium, the bacteria grew normally; inhibition zones become small while long chain acids were added in it. The overall trend is consistent with the results of yanglingmycin, whereby by decreasing the concentration of fatty acids added, the inhibition zone gradually increased. This implied that these antibacterial compounds influence fatty acid synthesis of the tested bacteria. Scanning electron microscopy results showed that the bacterial cell walls showed depressions, leading to cell content leakage. Compound 7h may damage cell membranes, leading to altered cell metabolism and the finally cell lysis and death. The antibacterial mechanism is similar to that of yanglingmycin.