Comparison of Chemical Profiles, Anti-Inflammatory Activity, and UPLC-Q-TOF/MS-Based Metabolomics in Endotoxic Fever Rats between Synthetic Borneol and Natural Borneol
Abstract
:1. Introduction
2. Results
2.1. Chemical Profiles of SB and NB by GC-MS Analysis
2.2. Anti-Inflammation Activity In Vitro
2.3. Serum Metabonomics of NB and SB in Endotoxic Fever Rats
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Chemical Profile by GC-MS Analysis
4.3. In Vitro Anti-Inflammation Effects on LPS-Induced RAW 264.7 Cells
4.4. Animal Handling for Endotoxic Fever Rat Model
4.5. Serum Pretreatment
4.6. UPLC-Q-TOF/MS Condition
4.7. Identification of Potential Biomarkers
4.8. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds synthetic borneol and natural borneol are available from the authors. |
Peak No. | R.T. (min) | Identity | Content% (SB) | Content% (NB) |
---|---|---|---|---|
1 | 2.16 | Camphene | 0.0016 | 0.0071 |
2 | 3.37 | Cineole | - | 0.0447 |
3 | 4.46 | Fenchone | 0.0038 | - |
4 | 6.04 | Camphor | 0.325 | 0.8095 |
5 | 6.73 | Iso-butylene | 0.0015 | - |
6 | 6.83 | α-fenchol | 0.4557 | 0.0116 |
7 | 6.93 | β-fenchyl alcohol | 0.3749 | - |
8 | 7.15 | fenchyl acetate | - | 0.0399 |
9 | 7.16 | exo-methyl-camphenilol | 0.0482 | - |
10 | 7.20 | 3-methyl-camphenilol | 0.0151 | 0.001 |
11 | 7.49 | m-menthene | 0.0128 | - |
12 | 8.13 | iso-borneol | 35.77 | 0.0918 |
13 | 8.49 | Borneol | 61.39 | 98.96 |
Peak No. | R.T. (min) | Formula | Mass (m/z) | Biomarkers |
---|---|---|---|---|
1 | 6.04 | C16H35NO2 | 274.2668 | Sphinganine |
2 | 1.14 | C5H11N | 85.0891 | Piperidine |
3 | 17.42 | C16H32O2 | 256.2402 | Fatty acid(14:0(10Me, 13Me)) |
4 | 9.64 | C26H52NO7P | 521.3554 | PC(0:1/18:1(9E)) |
5 | 15.90 | C20H34O2 | 306.2559 | 3β,5β-androstanediol |
6 | 1.12 | C7H13NO3 | 159.0895 | 5-acetamidopentanoate |
7 | 18.13 | C18H34O2 | 282.2559 | cis-2-octadecenoic acid |
8 | 21.44 | C40H79O7P | 702.5563 | PA(P-18:0/19:0) |
9 | 8.78 | C23H45NO4 | 399.3349 | Palmitoylcarnitine |
10 | 23.82 | C44H84NO8P | 785.6007 | PC(18:1(11Z)/18:1(9Z)) |
11 | 18.12 | C42H80NO8P | 757.5622 | PE(22:2(13Z, 16Z)/15:0) |
12 | 10.33 | C25H49NO4 | 427.3662 | DL-stearoylcanitine |
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Zou, L.; Zhang, Y.; Li, W.; Zhang, J.; Wang, D.; Fu, J.; Wang, P. Comparison of Chemical Profiles, Anti-Inflammatory Activity, and UPLC-Q-TOF/MS-Based Metabolomics in Endotoxic Fever Rats between Synthetic Borneol and Natural Borneol. Molecules 2017, 22, 1446. https://doi.org/10.3390/molecules22091446
Zou L, Zhang Y, Li W, Zhang J, Wang D, Fu J, Wang P. Comparison of Chemical Profiles, Anti-Inflammatory Activity, and UPLC-Q-TOF/MS-Based Metabolomics in Endotoxic Fever Rats between Synthetic Borneol and Natural Borneol. Molecules. 2017; 22(9):1446. https://doi.org/10.3390/molecules22091446
Chicago/Turabian StyleZou, Liang, Yan Zhang, Wei Li, Jinming Zhang, Dan Wang, Jia Fu, and Ping Wang. 2017. "Comparison of Chemical Profiles, Anti-Inflammatory Activity, and UPLC-Q-TOF/MS-Based Metabolomics in Endotoxic Fever Rats between Synthetic Borneol and Natural Borneol" Molecules 22, no. 9: 1446. https://doi.org/10.3390/molecules22091446