Development of a Mouse Model of Prostate Cancer Using the Sleeping Beauty Transposon and Electroporation
Abstract
:1. Introduction
2. Results
2.1. Tumor Observation
2.2. Microscopic Findings
2.3. Immunohistochemical Findings
2.4. Animal PET Imaging
2.5. Confirmation of Gene Expression Using Real-Time qRT-PCR
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Animals
4.3. Plasmid Construction
4.4. DNA Plasmid Injection
4.5. In Vivo Electroporation
4.6. Radiopharmaceutical Preparation and Animal PET Imaging
4.7. Histology and Immunohistochemistry
4.8. Detection of Gene Expression Using Quantitative Real Time-qRT-PCR
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the SB transposon vectors and SB transposase are not available from the authors. |
Tissue Marker | IHC Antibody | Type | Dilution | Reactivity |
---|---|---|---|---|
Epithelial tissue marker | Pan-cytokeratin | M | 1:3000 | + |
Cytokeratin 7 | P | 1:1000 | − | |
Cytokeratin 20 | M | 1:1000 | − | |
Muscular tissue marker | Myogenin | M | 1:1000 | − |
Desmin | M | 1:4000 | − | |
α-smooth muscle actin | M | 1:1000 | − | |
Hematopoietic cell marker | CD45 | M | 1:2000 | − |
CD163 | M | 1:2000 | − | |
CD68 | M | 1:2000 | − | |
Melanoma marker | Melanoma | M | 1:100 | − |
S100 | M | 1:400 | − | |
Adipose tissue marker | CDK4 | M | 1:1000 | − |
MDM2 | M | 1:1000 | − | |
Prostate marker | PSA | P | 1:100 | + |
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Choi, H.-J.; Lee, H.-B.; Jung, S.; Park, H.-K.; Jo, W.; Cho, S.-M.; Kim, W.-J.; Son, W.-C. Development of a Mouse Model of Prostate Cancer Using the Sleeping Beauty Transposon and Electroporation. Molecules 2018, 23, 1360. https://doi.org/10.3390/molecules23061360
Choi H-J, Lee H-B, Jung S, Park H-K, Jo W, Cho S-M, Kim W-J, Son W-C. Development of a Mouse Model of Prostate Cancer Using the Sleeping Beauty Transposon and Electroporation. Molecules. 2018; 23(6):1360. https://doi.org/10.3390/molecules23061360
Chicago/Turabian StyleChoi, Hyun-Ji, Han-Byul Lee, Sunyoung Jung, Hyun-Kyu Park, Woori Jo, Sung-Min Cho, Woo-Jin Kim, and Woo-Chan Son. 2018. "Development of a Mouse Model of Prostate Cancer Using the Sleeping Beauty Transposon and Electroporation" Molecules 23, no. 6: 1360. https://doi.org/10.3390/molecules23061360