Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. Substrates, Enzyme, and Equipment
3.2. SDS-Page Gel Analysis
3.3. Relative Hydrolysis
3.4. Sugar Analysis
3.5. Pre-Milling of Biomass
3.6. Determination of Optimal Water Loading
3.7. TLC Analysis of the Soluble Enzymatic Reaction Products
3.8. Reactions in Solution
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
No Bulk Water | With Bulk Water | |||
---|---|---|---|---|
Milling 30 min, RT | Aging 72 h, 55 °C | Shaking 30 min, RT | Shaking 72 h, 55 °C | |
Birchwood xylan | 39 ± 1% | 65 ± 1% | 14 ± 1% | 24 ± 3% |
Oat spelt xylan | 41 ± 1% | 68 ± 3% | 14 ± 1% | 26 ± 1% |
Sugarcane bagasse | 12 ± 6% | 73 ± 14% | 1.6 ± 0.5% | 4 ± 1% |
Wheat straw | 8 ± 4% | 84 ± 23% | 2.4 ± 0.2% | 5 ± 2% |
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Ostadjoo, S.; Hammerer, F.; Dietrich, K.; Dumont, M.-J.; Friscic, T.; Auclair, K. Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water. Molecules 2019, 24, 4206. https://doi.org/10.3390/molecules24234206
Ostadjoo S, Hammerer F, Dietrich K, Dumont M-J, Friscic T, Auclair K. Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water. Molecules. 2019; 24(23):4206. https://doi.org/10.3390/molecules24234206
Chicago/Turabian StyleOstadjoo, Shaghayegh, Fabien Hammerer, Karolin Dietrich, Marie-Josée Dumont, Tomislav Friscic, and Karine Auclair. 2019. "Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water" Molecules 24, no. 23: 4206. https://doi.org/10.3390/molecules24234206