Next Article in Journal
Lactoferrin in Aseptic and Septic Inflammation
Next Article in Special Issue
Design, Synthesis, and Evaluation of Monoamine Oxidase A Inhibitors–Indocyanine Dyes Conjugates as Targeted Antitumor Agents
Previous Article in Journal
A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 24
Issue 7
10.3390/molecules24071322
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

High Consistency of Structure-Based Design and X-Ray Crystallography: Design, Synthesis, Kinetic Evaluation and Crystallographic Binding Mode Determination of Biphenyl-N-acyl-β-d-Glucopyranosylamines as Glycogen Phosphorylase Inhibitors

by
Thomas Fischer
1,†,
Symeon M. Koulas
2,†,
Anastasia S. Tsagkarakou
2,†,
Efthimios Kyriakis
2,
George A. Stravodimos
2,
Vassiliki T. Skamnaki
2,
Panagiota G.V. Liggri
2,3,
Spyros E. Zographos
3,
Rainer Riedl
1,* and
Demetres D. Leonidas
2,*
1
Institute of Chemistry and Biotechnology, Center of Organic and Medicinal Chemistry, Zurich University of Applied Sciences, Einsiedlerstrasse 31, 8820 Wädenswil, Switzerland
2
Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 41500 Larissa, Greece
3
Institute of Biology, Pharmaceutical Chemistry and Biotechnology, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, Greece
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2019, 24(7), 1322; https://doi.org/10.3390/molecules24071322
Submission received: 15 March 2019 / Revised: 30 March 2019 / Accepted: 1 April 2019 / Published: 3 April 2019
(This article belongs to the Special Issue Drug Design)
Browse Figures
Review Reports Versions Notes

Abstract

:
Structure-based design and synthesis of two biphenyl-N-acyl-β-d-glucopyranosylamine derivatives as well as their assessment as inhibitors of human liver glycogen phosphorylase (hlGPa, a pharmaceutical target for type 2 diabetes) is presented. X-ray crystallography revealed the importance of structural water molecules and that the inhibitory efficacy correlates with the degree of disturbance caused by the inhibitor binding to a loop crucial for the catalytic mechanism. The in silico-derived models of the binding mode generated during the design process corresponded very well with the crystallographic data.

Graphical Abstract

1. Introduction

Glycogen phosphorylase (GP) is a validated pharmaceutical target for the discovery of new antidiabetic drugs [1,2]. GP is an allosteric enzyme that catalyses the first step of glycogen degradation in the liver and muscle to produce glucose-1-phosphate. The human hepatic enzyme (hlGPa) is responsible to maintain blood glucose homeostasis [3]. GP senses plasma levels of glucose to ensure that glycogenolysis is halted when glucose is abundant in plasma. This is achieved through allosteric binding of glucose to GP and stabilization of a conformation that enables the inactivation of the enzyme [4]. However, glucose is not a potent inhibitor of GP (Ki = 3.2 mM) [5] since blood glucose levels have to be maintained. Thus, the development of potent GP inhibitors was based on glucose derivatives by modifying glucose to generate inhibitors with better binding affinity [6]. Early structural studies proposed the modification of the β-1 position and the addition of suitable hydrophobic groups to exploit interactions with a hydrophobic pocket [7,8] in the direction of the β-anomeric substituent, adjacent to the active site formed mostly by hydrophobic residues (termed as the β-channel) [9]. A –CONH– linker between glucopyranose and the hydrophobic groups has proven to be particularly efficient since NH forms a hydrogen bond to the side chain of His377 [6]. One of the first attempts to designing potent GP inhibitors was to add a methyl or a phenyl group (1 and 2, Figure 1) that led to a 100- and 39.5-fold decrease of the Ki value, respectively compared to that of glucose [7].
Here, we present the structure-based design and organic synthesis of two new GP glucopyranosyl inhibitors as well as the kinetic and X-ray crystallography assessment of the new compounds.

2. Results

2.1. Molecular Modelling

We designed the two molecules 3 and 4, as depicted in Figure 2, as potential inhibitors of GP.
For the structure-based design of novel GP inhibitors, we examined the co-crystal structure of 2 bound to GP (PDB entry 3G2N) [9]. As is visible in Figure 3a, the glucopyranose moiety forms a network of hydrogen bonds with the amino acid residues His377, Asn484, Glu672 and Gly675 whereas the phenyl group points into a spacious cavity (β-channel) that provides sufficient room for further ligand growing. We hypothesized that an aromatic ring attached in para position of the phenyl group can interact with His341 by aromatic interactions for inhibitor enhancement. The 4-fluorophenyl unit in the meta position was intended to populate the β-channel and engage in constructive interactions with the amino acids Asn282 and His341 in close proximity. The strategic placement of a single fluorine atom is reported to have also positive effects on the pharmacokinetic profile of drug molecules, and by the formation of hydrogen bonds it can contribute to elevated inhibitor potency [10,11,12,13,14]. We performed docking experiments applying pharmacophore queries for the conservation of the hydrogen bonds formed by the glucopyranose residue and to maintain optimal ligand orientation.
Both postulated inhibitors could be docked into the cavity with the glucopyranose head group being anchored to fulfil the pharmacophore query. Compound 3 displayed two almost equally ranked docking poses that differ significantly in orientation by populating two opposite sites within the cavity. In the highest ranked pose (Figure 3b) the fluorine atom points towards the amino acids Asp339 and Ala383 and might form a water mediated interaction with one of the solvent molecules present in close proximity. In the second highest ranked docking pose, represented in Figure 3c, the fluorine penetrates the cavity towards Tyr280. In this pose, the fluorine containing phenyl ring overlaps with a water molecule that is present in the crystal structure PDB 3G2N which has to be replaced by the incoming ligand (solvent was set to inactive in the force field for the docking experiment). For compound 4 only one highly ranked orientation was obtained in the molecular modeling (Figure 3d) with the ligand being oriented in a straight line. We decided to synthesize the designed compounds to examine their inhibitory potential as well as their actual orientation in biological assays and crystallographic experiments.

2.2. Chemistry

Driven by our encouraging docking results we synthesized the designed molecules 3 and 4 according to the synthetic route summarized in Figure 4. The amino functionalized protected glucopyranoside derivative 6 could be obtained through hydrogenation of the commercially available azide 5. The formation of an amide bond between the amine 6 and either 4’-fluorobiphenyl-3-carboxylic acid or biphenyl-4-carbonylchloride afforded the intermediates 7 and 8. Elimination of the protecting acyl groups yielded the designed GP inhibitors 3 and 4.

2.3. Biological Evaluation

The synthesized compounds were examined in in vitro assays to determine their inhibitory potential against GP. Inhibition constant (Ki) values were measured using hlGPa (the pharmacologically relevant target) and the rabbit muscle enzymes (rmGPa and rmGPb) for comparison reasons since previously reported Ki values for other inhibitors were determined for the rabbit muscle enzymes. Production of rmGPa, rmGPb, and hlGPa, as well as enzyme kinetics experiments were performed according to previously described protocols [15]. As depicted in Table 1, both compounds 3 and 4 could be identified as GP inhibitors. They displayed competitive inhibition with respect to the substrate Glc-1-P (kinetics plots are presented in the Supplementary Materials, Figure S1). Within the series of tested molecules, 4 proved to be the most potent inhibitor with a Ki of 9.7 μM against rmGPb and 19.4 μM against the pharmacologically relevant hlGPa. Compared with the co-crystallized ligand 2 (PDB 3G2N), which was used for the design of the novel compounds, inhibitor 4 displayed elevated potency by almost one order of magnitude.

2.4. X-Ray Crystallography

Motivated by the positive results in the biological assays, we performed structural studies of the two novel inhibitors 3 and 4. The studies were performed using rmGPb crystals since they are easier to grow and the active site is fully conserved in terms of amino acid sequence and structural architecture compared to hlGPa [17,18]. Previous studies revealed that structural data deriving from rmGPb can be directly correlated to hlGPa [15,19,20].
Both inhibitors were found bound to the active site with the glucopyranose ring anchoring to the region known to bind glucose analogues in previous rmGPb-inhibitor complexes [2,21]. However, the two compounds bind significantly different at the enzyme active site and the biphenyl moieties point to different directions (Figure 5) as a result of their meta and para connection, respectively. As expected, the biphenyl fragment itself is not planar, and one benzene ring is twisted in relation to the other and the dihedral angle is 30° and 42° in 3 and 4, respectively. Upon binding to rmGPb 3 and 4 engage in 16 and 14 hydrogen bond interactions with protein residues in the active site when applying a distance cut off of 3.3 Å using the program CONTACT as implemented in CCP4 [22] (Table S2), respectively. The –CONH- linker in the 3 complex forms two hydrogen bonds, the N1 to the carbonyl oxygen of His377 and to the side chain of Asn284, both of which are not present in the 4 complex. Instead, in the 4 complex, atom O2 of the linker forms a hydrogen bond to the main chain amide of Leu136 (Figure 5; Table S2), a hydrogen bond previously observed for other acyl-glucopyranose derivative complexes.
The fluorine of 3 forms a halogen [13] bond to the side chain of Asn282 (Figure 5) and this interaction seems to govern the orientation of the biphenyl moiety within the active site of rmGPb. Superposition of the two complex structures onto the native unliganded rmGPb structure reveals that the binding of 3 triggers a significant conformational change of a loop composed by residues 282–289 (termed 280s loop) [4]. The root mean square distance for all atoms of residues 282–285 between the rmGPb-3 and rmGPb-4 complex structures is 0.9 Å, with Asn282, Asp283, and Asn284, being the residues with the greatest difference. The new conformation of the 280s loop brings the side chain of Asn282 to a halogen bonding distance [13] to F1 of 3 (Figure 6). This conformation is further stabilized through the formation of a hydrogen bond between the main chain carbonyl oxygen of Asp283 and the main chain amide of Phe285.

3. Discussion

Structural comparison of the rmGPb-4 complex with the rmGPb-2 complex [9] (Figure 7) reveals that although the glucopyranose atoms are at almost identical positions at the active site C1′ shifts by ~0.4 Å to best orient the biphenyl moiety (there is a 47° inclination between the phenyl rings of 2 and 4) and hence the hydrogen bond of N1 to the main chain carbonyl oxygen of His377 is lost (distance in the rmGPb-4 complex, 4.2 Å). However, this loss is compensated by the formation of a hydrogen bond of O2 to the main chain amide of Leu136. Therefore, it seems that the 15 additional van der Waals contacts (applying a distance cut off of 4.0 Å using the program CONTACT as implemented in CCP4 [22]) of the terminal phenyl group with the protein residues at the active site are the reason for the 8.3 times lower Ki value of 4 with respect to that of 2. Similarly, structural comparison of the rmGPb-4 complex and the rmGPb-1 complex [16] (Figure 7) also reveals a C1′ shift by ~0.6 Å with a resulting loss of the hydrogen bond of N1 to the main chain carbonyl oxygen of His377 which is compensated by the formation of a hydrogen bond between O2 and Leu136. The van der Waals interactions of the biphenyl moiety once again seem to be the reason for the 3.3 times difference between the Ki values of 4 and 1.
The biphenyl moiety in both inhibitors, 3 and 4, is involved in 23 van der Waals interactions within a distance cut off of 4.0 Å with protein residues at the β-pocket, in each protein ligand complex. Therefore, it seems that despite their different location within the active site, non-polar interactions support the binding of the biphenyl moiety. Furthermore, despite the fact that the two terminal phenyl groups of 3 and 4 are inclined by ~15° to each other, both are involved in cation-π stacking interactions to the imidazole ring of His341 (Figure 5). The hydrogen bonds of N1 in the 4 complex to Asn284 and His377 and the halogen bond of F1 to Asn282 are partly counterbalanced in the 3 complex by the hydrogen bond of O2 to Leu136. Thus, the structural basis of the variation in the inhibitory potency between 3 and 4 seems to lie within the energy cost associated with the significant conformational change of the 280s loop triggered by the binding of 3 but not by the binding of 4.
The observed crystallographic data correspond very well with our design strategy and our molecular docking experiments. The second highest ranked docking pose of 3 is oriented like the co-crystallized ligand, pointing its para fluorinated phenyl ring towards the β-channel (Figure 8a). For compound 4 the congruence is even more striking as the docked pose and the observed orientation in the co-crystal structure are identical. Even the torsion between the two phenyl rings is modelled correctly (Figure 8b). The orientation of 3 with the para fluorinated aromatic ring in the β-channel offers a further potential explanation for its diminished inhibitory potency compared to 4. Compared with the co-crystal structure PDB 3G2N, an ordered water molecule bound in this area by the formation of two hydrogen bonds with protein residues is displaced by 3. As this area of the pocket is rather polar, it is plausible that the displacement of a water molecule by a hydrophobic system declines the constructive interactions and therefore worsens the inhibitors potency.
Given the unexpected significant difference in the potency of the two inhibitors, which can be explained by the X-ray crystallography results, the present study highlights the importance of obtaining structural data in every step of the inhibitor optimization process.

4. Materials and Methods

4.1. General

All NMR spectra were recorded on a Bruker (Fällanden, Switzerland) AVANCE III HD 500 One Bay spectrometer with a magnetic field of 11.75 T. For 1H NMR spectra a frequency of 500 MHz resulted. Chemical shifts are reported in ppm from tetramethylsilane as internal standard. Data are reported as follows: chemical shift, multiplicity (s = singlet, d = doublet, t = triplet, q = quartet, quint. = quintet, br. = broad, m = multiplet), coupling constants (Hz), integration. For the 13C NMR spectra a frequency of 125 MHz resulted. Chemical shifts are reported in ppm from tetramethylsilane as internal standard, whereas fluorine coupling was observed, it was reported with multiplicity (d = doublet), coupling constants (Hz), and number of carbon atoms. The multiplicities of the signals were determined by DEPT measurements. Low-resolution mass spectrometry was performed on a MSQ Plus device (Thermo Scientific, Basel, Switzerland). NMR spectra of all synthesized compounds as well as LC-MS and HPLC spectra of the test compounds, graphs of the kinetics studies and crystallographic data can be found in the Supplementary Materials.

4.2. Chemistry

All reagents and solvents were purchased from Sigma Aldrich (Buchs, Switzerland), TCI (Zwijndrecht, Belgium) or Fluorochem (Hadfield, UK) and used as received. Solvents were stored over 4 Å molecular sieves.
2-(acetoxymethyl)-6-aminotetrahydro-2H-pyran-3,4,5-triyl triacetate (6; ZHAWOC6075): 1-Azido-1-deoxy-b-D-glucopyranoside tetraacetate (5) (0.55 g, 1.78 mmol) was dissolved in ethyl acetate (20 mL). Palladium 10% on activated charcoal (0.11 g, 0.1 mmol) was added and a hydrogen atmosphere was applied at 1 bar. After stirring at ambient temperature for 12 h the mixture was filtered over celite and concentrated in vacuum. The title compound 6 (0.49 g, 97% yield) was used in further synthesis without purification: 1H-NMR (500 MHz, [D6]DMSO, 25 °C, TMS): δ = 5.23 (t, J = 9.6 Hz, 1H), 4.82 (t, J = 10.0 Hz, 1H), 4.62 (dd, J = 9.6 Hz, 9.0 Hz, 1H), 4.29 (d, J = 7.6 Hz, 1H), 4.09 (dd, J = 12.3 Hz, 5.3 Hz, 1H), 3.97 (dd, J = 12.3 Hz, 2.4 Hz, 1H), 3.88 (ddd, J = 10.0 Hz, 5.3 Hz, 2.4 Hz, 1H), 2.54 (br. s, 2H), 2.19 (s, 3H), 1.97 (s, 3H), 1.97 (s, 3H), 1.93 (s, 3H) ppm. 13C-NMR (125 MHz, [D6]DMSO, 25 °C, TMS): δ = 170.54, 170.01, 196.83, 169.79, 84.58, 73.39, 72.69, 71.61, 69.22, 62.82, 21.06, 21.03, 20.87, 20.80 ppm. MS (m/z): 284 [M + H]+.
2-(acetoxymethyl)-6-(4’-fluorobiphenyl-3-ylcarboxamido)tetrahydro-2H-pyran-3,4,5-triyl triacetate (7; ZHAWOC6074): The amine 6 (100 mg, 0.353 mmol) and 4’-fluorobiphenyl-3-carboxylic acid (77 mg, 0.353 mmol) were dissolved in dimethylformamide (6 mL) and cooled to 0 °C. COMU (151 mg, 0.353 mmol) was added followed by diisopropylethylamine (0.12 mL) and the mixture was stirred at 0 °C. The reaction was stirred overnight and allowed to reach ambient temperature. Ethyl acetate (25 mL) was added and the organic phase was washed with 1N HCl (2 × 10 mL), NaHCO3 10% (2 × 10 mL) and brine (2 × 10 mL). The organic layer was dried over sodium sulphate and concentrated in vacuum. Purification by chromatography on silica gel (Gradient 0-100% ethyl acetate in cyclohexane) afforded the title compound 7 in 26% yield: 1H-NMR (500 MHz, CDCl3, 25 °C, TMS): δ = 7.99 (t, J = 1.7 Hz, 1H), 7.73–7.70 (m, 1H), 7.69-7.66 (m, 1H), 7.60–7.56 (m, 2H), 7.51 (t, J = 7.7 Hz, 1H), 7.18–7.13 (m, 2H), 5.47 (t, J = 9.2 Hz, 1H), 5.41 (t, J = 9.5 Hz, 1H), 5.13 (dd, J = 10.0 Hz, 9.5 Hz, 1H), 5.08 (t, J = 9.6 Hz, 1H), 4.35 (dd, J = 12.6 Hz, 4.4 Hz, 1H), 4.12 (dd, J = 12.6 Hz, 2.3 Hz, 1H), 3.93 (ddd, J = 10.0 Hz, 4.4 Hz, 2.3 Hz, 1H), 2.08 (s, 3H), 2.05 (s, 3H), 2.05 (s, 3H), 2.05 (s, 3H) ppm. 13C-NMR (125 MHz, CDCl3, 25 °C, TMS): δ = 171.61, 170.63, 169.88, 169.61, 167.04, 162.79 (d, J = 247.4 Hz, 1C), 140.89, 136.01 (d, J = 3.3 Hz, 1C), 133.42, 130.84, 129.29, 128.76 (d, J = 8.2, 2C), 126.12, 125.62, 115.88 (d, J = 21.5 Hz, 2C), 78.99, 73.66, 72.58, 70.90, 68.22, 61.64, 20.76, 20.74, 20.62, 20.61 ppm. MS (m/z): 546 [M + H]+.
2-(acetoxymethyl)-6-biphenyl-4-ylcarboxamidotetrahydro-2H-pyran-3,4,5-triyl triacetate (8; ZHAWOC6076): Under an argon atmosphere 6 (50 mg, 0.176 mmol) was dissolved in tetrahydrofuran (3 mL). Biphenyl-4-carbonylchloride (38 mg, 0.176 mmol) and diisopropylethylamine (0.05 mL) were added and the mixture was stirred at ambient temperature for 2 h. The solvent was removed in vacuum and the crude material was purified by chromatography on silica gel (Gradient: 0–100% methanol in dichloromethane) to obtain the title compound 8 in 75% yield: 1H-NMR (500 MHz, CDCl3, 25 °C, TMS): δ = 7.84–7.83 (m, 2H), 7.70–7.66 (m, 2H), 7.64–7.60 (m, 2H), 7.50–7.45 (m,2H), 7.43–7.38 (m, 1H), 7.15 (d, J = 9.1 Hz, 1H), 5.49 (t, J = 9.3 Hz, 1H), 5.49 (t, J = 9.5 Hz, 1H), 5.14 (t, J = 10.0, 1H), 5.10 (t, J = 9.5 Hz, 1H), 4.37 (dd, J = 12.7 Hz, 4.4 Hz, 1H), 4.13 (dd, J = 12.7 Hz, 2.2 Hz, 1H), 3.94 (ddd, J = 10.0 Hz, 4.4 Hz, 2.2 Hz, 1H), 2.10 (s, 3H), 2.07 (s, 9H) ppm. 13C-NMR (125 MHz, CDCl3, 25 °C, TMS): δ = 171.57, 170.63, 169.88, 169.61, 166.85, 145.19, 139.73, 131.35, 128.96, 128.19, 127.80, 127.38, 127.21, 78.97, 73.63, 72.63, 70.86, 68.25, 61.66, 20.75, 20.74, 20.62, 20.61 ppm. MS (m/z): 528 [M + H]+.
4’-fluoro-N-(3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)biphenyl-3-carboxamide (3; ZHAWOC6072): The acyl protected compound 7 (50 mg, 0.10 mmol) was dissolved in methanol (15 mL) and sodium methanolate (54 mg, 1.00 mmol in 1 mL methanol) was added and the mixture kept stirring at ambient temperature for 2h. After neutralization with Amberlyst 15 H+ form and further stirring for 5 min. the mixture was filtrated and the solvent was removed in vacuum. Purification by chromatography on reversed phase silica gel (Gradient 0–100% methanol in water) afforded the title compound 3 as a white solid with purity > 99.8% (0.02 g, 53% yield): 1H-NMR (500 MHz, [D6]DMSO, 25 °C, TMS): δ = 8.99 (d, J = 9.0 Hz, 1H), 8.19 (t, J = 1.6 Hz, 1H), 7.89 (dt, J = 7.8 Hz, 1.1 Hz, 1H), 7.85–7.79 (m, 3H), 7.58–7.54 (m, 1H), 7.37–7.30 (m, 2H), 5.05–4.89 (m, 4H), 4.52 (t, J = 5.6Hz, 1H), 3.72–3.65 (m, 1H), 3.48–3.41 (m, 1H), 3.37–3.31 (m, 1H), 3.25 (td, J = 8.8 Hz, 4.2 Hz, 1H), 3.20 (ddd, J = 9.4 Hz, 5.6 Hz, 2.0 Hz, 1H), 3.11 (td, J = 9.3 Hz, 4.8 Hz, 1H) ppm. 13C-NMR (125 MHz, [D6]DMSO, 25 °C, TMS): δ = 166.88, 162.53 (d, J = 244.8 Hz, 1C), 139.48, 136.45 (d, J = 3.2 Hz, 1C), 135.27, 130.04, 129.47, 129.39 (d, J = 8.2 Hz, 2C), 127.33, 126.06, 116.24, (d, J = 21.4 Hz, 2C), 80.79, 79.26, 77.99, 72.67, 70.52, 61.47 ppm. MS (m/z): 378 [M + H]+.
N-(3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)biphenyl-4-carboxamide (4; ZHAWOC6077): The acyl protected compound 8 (70 mg, 0.133 mmol) was dissolved in methanol (15 mL) and sodium methanolate (72 mg, 1.33 mmol in 1 mL methanol) was added and the mixture kept stirring at ambient temperature for 2 h. After neutralization with Amberlyst 15 H+ form and further stirring for 5 min. the mixture was filtrated and the solvent was removed in vacuum. Purification by chromatography on reversed phase silica gel (gradient 0–100% methanol in water) afforded the title compound 4 as a white solid with purity >99.8% (0.04 g, 73% yield): 1H-NMR (500 MHz, [D6]DMSO, 25 °C, TMS): δ = 8.89 (d, J = 8.9 Hz, 1H), 8.04–8.00 (m, 2H), 7.81–7.77 (m, 2H), 7.76–7.72 (m, 2H), 7.52–7.47 (m, 2H), 7.44–7.39 (m, 1H), 5.04–4.89 (m, 4H), 4.52 (t, J = 5.7 Hz, 1H), 3.69 (m, 1H), 3.45 (dt, J = 11.5 Hz, 5.6 Hz, 1H), 3.36 (td, J = 9.0 Hz, 4.7 Hz, 1H), 3.25 (td, J = 8.8 Hz, 4.4 Hz, 1H), 3.19 (ddd, J = 9.7 Hz, 5.6 Hz, 2.1 Hz, 1H), 3.11 (td, J = 9.2 Hz, 5.0 Hz, 1H) ppm. 13C-NMR (125 MHz, [D6]DMSO, 25 °C, TMS): δ = 166.74, 143.40, 139.58, 133.41, 129.51, 128.79, 128.57, 127.36, 126.86, 80.81, 79.26, 78.08, 72.56, 70.55, 61.48 ppm. MS (m/z): 360 [M + H]+.

4.3. In Silico Studies

Molecular modelling experiments were performed using the Molecular Operating Environment MOE 2015.10 from Chemical Computing Group. Co-crystal structures of GP are available from the Protein Data Bank. For the actual work pdb entry: 3G2N was selected for the computational studies. In MOE the pocket was prepared for the dockings via the Protonate 3D method applying the default values for temperature 300 K, pH 7 and salt 0.1. The ligands to be docked to the protein were imported from SD files to receive a MOE compatible molecular database. As the SD files did not contain 3D coordinates, they were generated directly using MOE rebuild3D with an RMSD gradient of 0.1. For docking experiments the Amber10:EHT force field was used [23,24]. The pharmacophore placement was applied with a rigid receptor. The docked poses were subsequently analysed with respect to their scores and interactions with the target enzyme.

4.4. Kinetics

Rabbit muscle GPb (rmGPb) was purified from rabbit skeletal muscles following the protocol developed by Fischer and Krebs [25] with a slight modification (l-cysteine was replaced with 2-mercaptoethanol). Human liver GPb (hlGPb) was produced as described previously [15]. rmGPa and hlGPa were prepared by phosphorylation of rmGPb and hlGPb, respectively, performed using a truncated form of the γ (catalytic) subunit of rabbit skeletal muscle phosphorylase kinase produced as described previously [26].
Kinetic studies were performed at 30 °C in the direction of glycogen synthesis by measuring the inorganic phosphate released in the reaction using the method by Saheki et al. [27] 3 µg/mL rmGPb, rmGPa, or 1 µg/mL hlGPa were assayed in a 30 mM imidazole/HCl buffer (pH 6.8) containing 60 mM KCl, 0.6 mM EDTA, and 0.6 mM dithiothreitol using constant concentrations of glycogen (0.2% w/v), AMP (1 mM; only for the rmGPb experiments), and various concentrations of Glc-1-P (2, 3, 4, 6, and 10 mM for rmGP and 1, 2, 3, 4, and 6 mM for hlGPa) and inhibitors. Briefly, absorption at 850 nm of each sample is transformed to µmoles of phosphates by using a standard curve. Initial velocities were calculated from the pseudo-first order rate constants (k) using the first-order rate equation ([A]=[A]0 ∙ ekt) where [A]0 and [A] are the initial and the sample’s concentration of substrate at various times, and t is the corresponding time (min). The apparent KM values (KM(app.)) are then calculated by plotting pseudo-first order rate constants (k) vs. [Glc-1-P] using the Michaelis–Menten equation. The inhibition constant (Ki) values were then calculated from the intercept to horizontal axis of the plot of KM(app.) vs. [inhibitor] using non-linear regression program GRAFIT [28] and an explicit value for the standard deviation of each point.

4.5. X-Ray Crystallography

Tetragonal (space group P43212) T state rmGPb crystals were grown by the batch method. Briefly, an rmGPb (100 mg/mL) solution in a 50 mM β-glycerol phosphate buffer pH 6.8, supplemented with 50 mM β-mercaptoethanol and 1 mM EDTA was dialyzed overnight at 4 °C against a solution of a 10 mM BES (N,N-bis-(2-hydroxyethyl)-2-aminoethane sulfonic acid/NaOH) buffer pH 6.7, supplemented with 0.1 mM EDTA, 0.02% (w/v) sodium azide and active charcoal (rmGPb—charcoal ratio 1:1.2) to remove any nucleotides bound to the enzyme. The enzyme solution was then diluted to 25–30 mg/mL with the dialysis buffer and the addition of spermine and DTT to final concentration of 1 and 3 mM, respectively. Microseeds, prepared from previously grown rmGPb crystals, were also added in the crystallization solution. The final crystallization solution was placed in small tubes (diameter 3 mm; length 3 cm) and left at 16 °C. rmGPb crystals appeared after 3–4 days. X-ray crystallographic binding studies were performed by diffusion of either 3 or 4 (1 mM; 24 h), solution in the crystallization media supplemented with 10% (v/v) DMSO in preformed rmGPb crystals at room temperature prior to data collection. X-ray diffraction data were collected using a Cu X-ray microfocus source (Oxford Diffraction SuperNova) equipped with a 4-kappa goniometer and the ATLAS CCD (135 mm) detector at room temperature. Crystal orientation, integration of reflections, inter-frame scaling, partial reflection summation, and data reduction was performed by the program CrysalisPro (Agilent Technologies UK Ltd.) [29]. Scaling and merging of intensities were performed by Aimless [30] and the optimum resolution was selected based on the CC1/2 criterion [30]. Crystallographic refinement of the complexes was performed by maximum-likelihood methods using REFMAC [31]. The starting model employed for the refinement of the complexes was the structure of the native T state rmGPb complex determined at 1.9 Å resolution (Leonidas et al., unpublished results). Ligand molecule coordinates and topologies were constructed using AceDRG [32] within Coot [33] and they were fitted to the electron density maps after adjustment of their torsion angles. A summary of the data processing and refinement statistics for the inhibitor complex structures is given in Table S1 in the Supplementary Materials. The validity of the refinement procedure was checked using the PDB_REDO server [34]. As there were more than five reflections per atom available, both an isotropic and an anisotropic B-factor model were considered, and the isotropic B-factor model was selected based on the Hamilton R ratio test. A TLS model for grouped atom movement with one TLS group was used. The stereochemistry of the protein residues was validated by MolProbity [35]. Figures were prepared with CCP4 Molecular Graphics [36]. The coordinates of the new structures have been deposited with the RCSB Protein Data Bank (http://www.rcsb.org/pdb) with codes presented in Table S1.

5. Conclusions

Two novel inhibitors of GP were structure-based designed in silico, synthesized and evaluated towards their inhibitory properties from a kinetic and crystallographic point of view. Both inhibitors displayed higher potency towards rmGPb compared to the co-crystallized template ligand that was employed as the starting point for our targeted design. With a Ki of 9.7 μM for the rabbit muscle enzyme the most potent inhibitor was almost one order of magnitude more potent than the template compound. The pathologically relevant human target enzyme hlGPa was inhibited with a Ki of 19.4 μM. Crystallographic studies of the two inhibitors revealed that they bind as expected to the enzyme with the glucopyranose moiety anchored by the formation of multiple hydrogen bonds. Nevertheless, deviations were observed for the enzyme structure in the 280 s loop upon binding the two inhibitors. The resulting differences in constructive interactions to the inhibitor serve as the basis to explain the difference in potency of the two inhibitors. Comparison of the computationally derived ligand poses with the effectively determined coordinates obtained by X-ray crystallography demonstrated very high similarity, confirming the validity of the in silico drug design strategy.

Supplementary Materials

Supplementary materials related to this article, including complete analytical data of the synthesized compounds, kinetics plots and crystallographic data, are available online.

Author Contributions

Conceptualization: R.R. and D.D.L.; formal analysis: T.F., S.M.K., A.S.T., E.K., G.A.S., V.T.S., P.G.V.L., and S.E.Z.; funding acquisition: R.R. and D.D.L.; investigation: T.F., S.M.K., A.S.T., E.K., G.A.S., V.T.S., P.G.V.L., and S.E.Z.; project administration: R.R. and D.D.L.; supervision: R.R. and D.D.L.; validation: T.F., S.M.K., A.S.T., E.K., G.A.S., V.T.S., P.G.V.L., S.E.Z., R.R., and D.D.L.; visualization, T.F., E.K., V.T.S., P.G.V.L., and S.E.Z.; writing—original draft: T.F., E.K., V.T.S., R.R., and D.D.L.; writing—review and editing: T.F., E.K., V.T.S., R.R., and D.D.L.

Funding

This work was supported in part by the Postgraduate Program ‘‘Application of Molecular Biology-Molecular Genetics-Molecular Markers”, Department of Biochemistry and Biotechnology, University of Thessaly. E.K. would like to acknowledge support by Greece and the European Union (European Social Fund- ESF) through the Operational Program «Human Resources Development, Education and Lifelong Learning» in the context of the project “Strengthening Human Resources Research Potential via Doctorate Research” (MIS-5000432), implemented by the State Scholarships Foundation (ΙΚΥ). T.F. and R.R. are grateful to the Zurich University of Applied Sciences (ZHAW) for financial support. The APC was funded by the Zurich University of Applied Sciences (ZHAW) and by discount vouchers for publication in MDPI journals from R.R. and D.D.L.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Donnier-Maréchal, M.; Vidal, S. Glycogen phosphorylase inhibitors: A patent review (2013–2015). Expert Opin. Ther. Pat. 2016, 26, 199–212. [Google Scholar]
  2. Hayes, J.M.; Kantsadi, A.L.; Leonidas, D.D. Natural products and their derivatives as inhibitors of glycogen phosphorylase: Potential treatment for type 2 diabetes. Phytochem. Rev. 2014, 13, 471–498. [Google Scholar]
  3. Petersen, M.C.; Vatner, D.F.; Shulman, G.I. Regulation of hepatic glucose metabolism in health and disease. Nat. Rev. Endocrinol. 2017, 13, 572–587. [Google Scholar] [PubMed]
  4. Oikonomakos, N.G. Glycogen phosphorylase as a molecular target for type 2 diabetes therapy. Curr. Protein Pept. Sci. 2002, 3, 561–586. [Google Scholar] [CrossRef]
  5. Leonidas, D.D.; Oikonomakos, N.G.; Papageorgiou, A.C.; Xenakis, A.; Cazianis, C.T.; Bem, F. The ammonium sulfate activation of phosphorylase b. FEBS Lett. 1990, 261, 23–27. [Google Scholar]
  6. Somsák, L. Glucose derived inhibitors of glycogen phosphorylase. Comptes Rendus Chim. 2011, 14, 211–223. [Google Scholar] [CrossRef]
  7. Watson, K.A.; Mitchell, E.P.; Johnson, L.N.; Cruciani, G.; Son, J.C.; Bichard, C.J.; Fleet, G.W.; Oikonomakos, N.G.; Kontou, M.; Zographos, S.E. Glucose analogue inhibitors of glycogen phosphorylase: From crystallographic analysis to drug prediction using GRID force-field and GOLPE variable selection. Acta Crystallogr. D Biol. Crystallogr. 1995, 51, 458–472. [Google Scholar]
  8. Watson, K.A.; Mitchell, E.P.; Johnson, L.N.; Bichard, C.J.F.; Orchard, M.G.; Fleet, G.W.J.; Oikonomakos, N.G.; Leonidas, D.D.; Son, J.C. Design of Inhibitors of Glycogen Phosphorylase: A Study of .alpha.- and .beta.-C-Glucosides and 1-Thio-.beta.-D-glucose Compounds. Biochemistry 1994, 33, 5745–5758. [Google Scholar]
  9. Chrysina, E.D.; Bokor, É.; Alexacou, K.M.; Charavgi, M.D.; Oikonomakos, G.N.; Zographos, S.E.; Leonidas, D.D.; Oikonomakos, N.G.; Somsák, L. Amide-1,2,3-triazole bioisosterism: The glycogen phosphorylase case. Tetrahedron Asymmetry 2009, 20, 733–740. [Google Scholar]
  10. Kerekes, A.D.; Esposite, S.J.; Doll, R.J.; Tagat, J.R.; Yu, T.; Xiao, Y.; Zhang, Y.; Prelusky, D.B.; Tevar, S.; Gray, K.; et al. Aurora Kinase Inhibitors Based on the Imidazo[1,2-a]pyrazine Core: Fluorine and Deuterium Incorporation Improve Oral Absorption and Exposure. J. Med. Chem. 2011, 54, 201–210. [Google Scholar] [PubMed]
  11. Yamazaki, S.; Shen, Z.; Jiang, Y.; Smith, B.J.; Vicini, P. Application of Target-Mediated Drug Disposition Model to Small Molecule Heat Shock Protein 90 Inhibitors. Drug Metab. Dispos. 2013, 41, 1285–1294. [Google Scholar] [CrossRef] [PubMed]
  12. Howard, J.A.K.; Hoy, V.J.; O’Hagan, D.; Smith, G.T. How good is fluorine as a hydrogen bond acceptor? Tetrahedron 1996, 52, 12613–12622. [Google Scholar]
  13. Auffinger, P.; Hays, F.A.; Westhof, E.; Ho, P.S. Halogen bonds in biological molecules. Proc. Natl. Acad. Sci. USA 2004, 101, 16789–16794. [Google Scholar] [PubMed]
  14. Fischer, T.; Riedl, R. Targeted Fluoro Positioning for the Discovery of a Potent and Highly Selective Matrix Metalloproteinase Inhibitor. ChemistryOpen 2017, 6, 192–195. [Google Scholar]
  15. Kyriakis, E.; Solovou, T.G.A.; Kun, S.; Czifrák, K.; Szőcs, B.; Juhász, L.; Bokor, É.; Stravodimos, G.A.; Kantsadi, A.L.; Chatzileontiadou, D.S.M.; et al. Probing the β-pocket of the active site of human liver glycogen phosphorylase with 3-(C-β-d-glucopyranosyl)-5-(4-substituted-phenyl)-1, 2, 4-triazole inhibitors. Bioorganic Chem. 2018, 77, 485–493. [Google Scholar]
  16. Oikonomakos, N.G.; Kontou, M.; Zographos, S.E.; Watson, K.A.; Johnson, L.N.; Bichard, C.J.F.; Fleet, G.W.J.; Acharya, K.R. N-acetyl-β-D-glucopyranosylamine: A potent T-state inhibitor of glycogen phosphorylase. A comparison with α-D-glucose. Protein Sci. 1995, 4, 2469–2477. [Google Scholar]
  17. Newgard, C.B.; Nakano, K.; Hwang, P.K.; Fletterick, R.J. Sequence analysis of the cDNA encoding human liver glycogen phosphorylase reveals tissue-specific codon usage. Proc. Natl. Acad. Sci. USA 1986, 83, 8132–8136. [Google Scholar]
  18. Rath, V.L.; Ammirati, M.; LeMotte, P.K.; Fennell, K.F.; Mansour, M.N.; Danley, D.E.; Hynes, T.R.; Schulte, G.K.; Wasilko, D.J.; Pandit, J. Activation of Human Liver Glycogen Phosphorylase by Alteration of the Secondary Structure and Packing of the Catalytic Core. Mol. Cell 2000, 6, 139–148. [Google Scholar] [CrossRef]
  19. Kyriakis, E.; Stravodimos, G.A.; Kantsadi, A.L.; Chatzileontiadou, D.S.; Skamnaki, V.T.; Leonidas, D.D. Natural flavonoids as antidiabetic agents. The binding of gallic and ellagic acids to glycogen phosphorylase b. FEBS Lett. 2015, 589, 1787–1794. [Google Scholar] [PubMed]
  20. Kantsadi, A.L.; Bokor, É.; Kun, S.; Stravodimos, G.A.; Chatzileontiadou, D.S.M.; Leonidas, D.D.; Juhász-Tóth, É.; Szakács, A.; Batta, G.; Docsa, T.; et al. Synthetic, enzyme kinetic, and protein crystallographic studies of C-β-d-glucopyranosyl pyrroles and imidazoles reveal and explain low nanomolar inhibition of human liver glycogen phosphorylase. Eur. J. Med. Chem. 2016, 123, 737–745. [Google Scholar]
  21. Somsák, L.; Czifrák, K.; Tóth, M.; Bokor, E.; Chrysina, E.D.; Alexacou, K.-M.; Hayes, J.M.; Tiraidis, C.; Lazoura, E.; Leonidas, D.D.; et al. New inhibitors of glycogen phosphorylase as potential antidiabetic agents. Curr. Med. Chem. 2008, 15, 2933–2983. [Google Scholar] [PubMed]
  22. Project, C.C. Number 4 The CCP4 suite: Programs for protein crystallography. Acta Crystallogr. D Biol. Crystallogr. 1994, 50, 760–763. [Google Scholar]
  23. Case, D.A.; Cheatham, T.E.; Darden, T.; Gohlke, H.; Luo, R.; Merz, K.M.; Onufriev, A.; Simmerling, C.; Wang, B.; Woods, R.J. The Amber biomolecular simulation programs. J. Comput. Chem. 2005, 26, 1668–1688. [Google Scholar] [PubMed]
  24. Gerber, P.R.; Müller, K. MAB, a generally applicable molecular force field for structure modelling in medicinal chemistry. J. Comput. Aided Mol. Des. 1995, 9, 251–268. [Google Scholar]
  25. Fischer, E.H.; Krebs, E.G. [49a] Muscle phosphorylase b: x Glucose-1-phosphate+ Gn ⇄ Gn+x + x inorganic phosphate(where Gn designates glycogen containing n glucose residues). In Methods in Enzymology; Academic Press: Boston, MA, USA, 1962; Volume 5, pp. 369–373. [Google Scholar]
  26. Hayes, J.M.; Skamnaki, V.T.; Archontis, G.; Lamprakis, C.; Sarrou, J.; Bischler, N.; Skaltsounis, A.-L.; Zographos, S.E.; Oikonomakos, N.G. Kinetics, in silico docking, molecular dynamics, and MM-GBSA binding studies on prototype indirubins, KT5720, and staurosporine as phosphorylase kinase ATP-binding site inhibitors: The role of water molecules examined. Proteins Struct. Funct. Bioinforma. 2011, 79, 703–719. [Google Scholar]
  27. Saheki, S.; Takeda, A.; Shimazu, T. Assay of inorganic phosphate in the mild pH range, suitable for measurement of glycogen phosphorylase activity. Anal. Biochem. 1985, 148, 277–281. [Google Scholar]
  28. Leatherbarrow, R.J. GraFit Version 6.0; Erithacus Software Limited: Staines, UK, 2007. [Google Scholar]
  29. Agilent Technologies UK Ltd. CrysAlisPro Software System, 1.171.35.11; Agilent Technologies UK Ltd.: Oxford, UK, 2011. [Google Scholar]
  30. Evans, P.R.; Murshudov, G.N. How good are my data and what is the resolution? Acta Crystallogr. D Biol. Crystallogr. 2013, 69, 1204–1214. [Google Scholar]
  31. Vagin, A.A.; Steiner, R.A.; Lebedev, A.A.; Potterton, L.; McNicholas, S.; Long, F.; Murshudov, G.N. REFMAC5 dictionary: Organization of prior chemical knowledge and guidelines for its use. Acta Crystallogr. D Biol. Crystallogr. 2004, 60, 2184–2195. [Google Scholar] [PubMed]
  32. Long, F.; Nicholls, R.A.; Emsley, P.; Gražulis, S.; Merkys, A.; Vaitkus, A.; Murshudov, G.N. AceDRG: A stereochemical description generator for ligands. Acta Crystallogr. Sect. Struct. Biol. 2017, 73, 112–122. [Google Scholar]
  33. Emsley, P.; Lohkamp, B.; Scott, W.G.; Cowtan, K. Features and development of Coot. Acta Crystallogr. D Biol. Crystallogr. 2010, 66, 486–501. [Google Scholar] [PubMed]
  34. Joosten, R.P.; Long, F.; Murshudov, G.N.; Perrakis, A. The PDB_REDO server for macromolecular structure model optimization. IUCrJ 2014, 1, 213–220. [Google Scholar] [CrossRef] [PubMed]
  35. Chen, V.B.; Arendall, W.B.; Headd, J.J.; Keedy, D.A.; Immormino, R.M.; Kapral, G.J.; Murray, L.W.; Richardson, J.S.; Richardson, D.C. MolProbity: All-atom structure validation for macromolecular crystallography. Acta Crystallogr. D Biol. Crystallogr. 2010, 66, 12–21. [Google Scholar] [PubMed]
  36. McNicholas, S.; Potterton, E.; Wilson, K.S.; Noble, M.E.M. Presenting your structures: The CCP4mg molecular-graphics software. Acta Crystallogr. D Biol. Crystallogr. 2011, 67, 386–394. [Google Scholar] [PubMed]
Sample Availability: Samples of the compounds 3 and 4 are available from the authors.
Molecules 24 01322 g001 550
Figure 1. Previously described glucopyranosyl containing GP inhibitors 1 and 2.
Figure 1. Previously described glucopyranosyl containing GP inhibitors 1 and 2.
Molecules 24 01322 g001
Molecules 24 01322 g002 550
Figure 2. Structure-based designed GP inhibitors containing a para fluorinated phenyl group in the meta position (3), and with a phenyl residue in para position (4).
Figure 2. Structure-based designed GP inhibitors containing a para fluorinated phenyl group in the meta position (3), and with a phenyl residue in para position (4).
Molecules 24 01322 g002
Molecules 24 01322 g003 550
Figure 3. (a) Co-crystal structure PDB 3G2N; (b) compound 3 docked to PDB 3G2N (highest scored pose; −9.1160); (c) compound 3 docked to PDB 3G2N (second highest scored pose; −8.9981); and (d) compound 4 docked to PDB 3G2N (highest scored pose; −9.4502), co-crystallized ligand with carbons in green, docked ligands with carbons in yellow.
Figure 3. (a) Co-crystal structure PDB 3G2N; (b) compound 3 docked to PDB 3G2N (highest scored pose; −9.1160); (c) compound 3 docked to PDB 3G2N (second highest scored pose; −8.9981); and (d) compound 4 docked to PDB 3G2N (highest scored pose; −9.4502), co-crystallized ligand with carbons in green, docked ligands with carbons in yellow.
Molecules 24 01322 g003
Molecules 24 01322 g004 550
Figure 4. Synthesis of the designed compounds 3 and 4. (a) Pd/C 10%, hydrogen, ethyl acetate, RT, 12 h, 97%; (b) 4’-fluorobiphenyl-3-carboxylic acid, COMU, DIPEA, DMF, 0 °C → RT, 12 h, 26%; (c) biphenyl-4-carbonylchloride, DIPEA, THF, RT, 2 h, 75%; (d) NaOMe, methanol, RT, 2 h, 3: 53%, 4: 73%.
Figure 4. Synthesis of the designed compounds 3 and 4. (a) Pd/C 10%, hydrogen, ethyl acetate, RT, 12 h, 97%; (b) 4’-fluorobiphenyl-3-carboxylic acid, COMU, DIPEA, DMF, 0 °C → RT, 12 h, 26%; (c) biphenyl-4-carbonylchloride, DIPEA, THF, RT, 2 h, 75%; (d) NaOMe, methanol, RT, 2 h, 3: 53%, 4: 73%.
Molecules 24 01322 g004
Molecules 24 01322 g005 550
Figure 5. The binding mode of 4 (left) and 3 (right) at the active site of rmGPb. The inhibitor is shown in thick sticks, hydrogen bonds as dashed lines, and water molecules as black spheres.
Figure 5. The binding mode of 4 (left) and 3 (right) at the active site of rmGPb. The inhibitor is shown in thick sticks, hydrogen bonds as dashed lines, and water molecules as black spheres.
Molecules 24 01322 g005
Molecules 24 01322 g006 550
Figure 6. Stereo diagram of the superposition of the rmGPb-3 complex (brown) onto the rmGPb-4 complex (green) showing the different conformations of the 280s loop.
Figure 6. Stereo diagram of the superposition of the rmGPb-3 complex (brown) onto the rmGPb-4 complex (green) showing the different conformations of the 280s loop.
Molecules 24 01322 g006
Molecules 24 01322 g007 550
Figure 7. Superposition of the rmGPb-3 complex (brown) onto the rmGPb-2 (left, green)) and the rmGPb-1 (right, grey) complexes.
Figure 7. Superposition of the rmGPb-3 complex (brown) onto the rmGPb-2 (left, green)) and the rmGPb-1 (right, grey) complexes.
Molecules 24 01322 g007
Molecules 24 01322 g008 550
Figure 8. (a) Overlay of the second highest ranked docking pose of 3 in PDB 3G2N with carbon atoms in yellow, and the crystal structure of 3 in GP with carbon atoms in green, (b) overlay of the highest ranked docking pose of 4 in PDB 3G2N with carbon atoms in yellow, and the crystal structure of 4 in GP with carbon atoms in green.
Figure 8. (a) Overlay of the second highest ranked docking pose of 3 in PDB 3G2N with carbon atoms in yellow, and the crystal structure of 3 in GP with carbon atoms in green, (b) overlay of the highest ranked docking pose of 4 in PDB 3G2N with carbon atoms in yellow, and the crystal structure of 4 in GP with carbon atoms in green.
Molecules 24 01322 g008
Table
Table 1. Inhibitory data for glycogen phosphorylases.
Table 1. Inhibitory data for glycogen phosphorylases.
InhibitorStructureKi [μM]
hlGParmGParmGPb
1 Molecules 24 01322 i001n.r. 135 ± 1 [16]32 ± 1 [16]
2 Molecules 24 01322 i002n.r. 1n.r. 181 ± 7 [7]
32 Molecules 24 01322 i00345.6 ± 1.831.4 ± 2.256.1 ± 4.4
42 Molecules 24 01322 i00419.4 ± 0.212.8 ± 0.69.7 ± 0.7
1 Not reported; 2 crystallographic numbering.

Share and Cite

MDPI and ACS Style

Fischer, T.; Koulas, S.M.; Tsagkarakou, A.S.; Kyriakis, E.; Stravodimos, G.A.; Skamnaki, V.T.; Liggri, P.G.V.; Zographos, S.E.; Riedl, R.; Leonidas, D.D. High Consistency of Structure-Based Design and X-Ray Crystallography: Design, Synthesis, Kinetic Evaluation and Crystallographic Binding Mode Determination of Biphenyl-N-acyl-β-d-Glucopyranosylamines as Glycogen Phosphorylase Inhibitors. Molecules 2019, 24, 1322. https://doi.org/10.3390/molecules24071322

AMA Style

Fischer T, Koulas SM, Tsagkarakou AS, Kyriakis E, Stravodimos GA, Skamnaki VT, Liggri PGV, Zographos SE, Riedl R, Leonidas DD. High Consistency of Structure-Based Design and X-Ray Crystallography: Design, Synthesis, Kinetic Evaluation and Crystallographic Binding Mode Determination of Biphenyl-N-acyl-β-d-Glucopyranosylamines as Glycogen Phosphorylase Inhibitors. Molecules. 2019; 24(7):1322. https://doi.org/10.3390/molecules24071322

Chicago/Turabian Style

Fischer, Thomas, Symeon M. Koulas, Anastasia S. Tsagkarakou, Efthimios Kyriakis, George A. Stravodimos, Vassiliki T. Skamnaki, Panagiota G.V. Liggri, Spyros E. Zographos, Rainer Riedl, and Demetres D. Leonidas. 2019. "High Consistency of Structure-Based Design and X-Ray Crystallography: Design, Synthesis, Kinetic Evaluation and Crystallographic Binding Mode Determination of Biphenyl-N-acyl-β-d-Glucopyranosylamines as Glycogen Phosphorylase Inhibitors" Molecules 24, no. 7: 1322. https://doi.org/10.3390/molecules24071322

Article Metrics

Back to TopTop