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Article

The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet

by
Todd W. Mitchell
1,*,
Nigel Turner
2,
Paul L. Else
1,
Anthony J. Hulbert
3,
John A. Hawley
4,
Jong Sam Lee
5,
Clinton R. Bruce
6 and
Stephen J. Blanksby
7
1
School of Health Sciences, University of Wollongong, NSW 2522, Australia
2
Garvan Institute of Medical Research, Sydney, NSW 2010, Australia
3
School of Biological Sciences, University of Wollongong, NSW 2522, Australia
4
School of Medical Sciences, RMIT University, Bundoora, Victoria 3083, Australia
5
Department of Physical Education, Daegu University, Daegu, South Korea
6
Baker IDI Heart & Diabetes Institute, Melbourne, Victoria 3004 Australia
7
School of Chemistry, University of Wollongong, NSW 2522, Australia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2010, 11(10), 3954-3964; https://doi.org/10.3390/ijms11103954
Submission received: 7 September 2010 / Revised: 8 October 2010 / Accepted: 12 October 2010 / Published: 15 October 2010
(This article belongs to the Special Issue Phospholipids: Molecular Sciences)
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Abstract

The aim of this study was to examine the effect of endurance training on skeletal muscle phospholipid molecular species from high-fat fed rats. Twelve female Sprague-Dawley rats were fed a high-fat diet (78.1% energy). The rats were randomly divided into two groups, a sedentary control group and a trained group (125 min of treadmill running at 8 m/min, 4 days/wk for 4 weeks). Forty-eight hours after their last training bout phospholipids were extracted from the red and white vastus lateralis and analyzed by electrospray-ionization mass spectrometry. Exercise training was associated with significant alterations in the relative abundance of a number of phospholipid molecular species. These changes were more prominent in red vastus lateralis than white vastus lateralis. The largest observed change was an increase of ~30% in the abundance of 1-palmitoyl-2-linoleoyl phosphatidylcholine ions in oxidative fibers. Reductions in the relative abundance of a number of phospholipids containing long-chain n-3 polyunsaturated fatty acids were also observed. These data suggest a possible reduction in phospholipid remodeling in the trained animals. This results in a decrease in the phospholipid n-3 to n-6 ratio that may in turn influence endurance capacity.
Keywords: electrospray-ionization mass spectrometry; endurance; exercise training; fatty acids; lipidomics electrospray-ionization mass spectrometry; endurance; exercise training; fatty acids; lipidomics

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MDPI and ACS Style

Mitchell, T.W.; Turner, N.; Else, P.L.; Hulbert, A.J.; Hawley, J.A.; Lee, J.S.; Bruce, C.R.; Blanksby, S.J. The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet. Int. J. Mol. Sci. 2010, 11, 3954-3964. https://doi.org/10.3390/ijms11103954

AMA Style

Mitchell TW, Turner N, Else PL, Hulbert AJ, Hawley JA, Lee JS, Bruce CR, Blanksby SJ. The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet. International Journal of Molecular Sciences. 2010; 11(10):3954-3964. https://doi.org/10.3390/ijms11103954

Chicago/Turabian Style

Mitchell, Todd W., Nigel Turner, Paul L. Else, Anthony J. Hulbert, John A. Hawley, Jong Sam Lee, Clinton R. Bruce, and Stephen J. Blanksby. 2010. "The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet" International Journal of Molecular Sciences 11, no. 10: 3954-3964. https://doi.org/10.3390/ijms11103954

APA Style

Mitchell, T. W., Turner, N., Else, P. L., Hulbert, A. J., Hawley, J. A., Lee, J. S., Bruce, C. R., & Blanksby, S. J. (2010). The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet. International Journal of Molecular Sciences, 11(10), 3954-3964. https://doi.org/10.3390/ijms11103954

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