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Int. J. Mol. Sci. 2012, 13(11), 14889-14897; doi:10.3390/ijms131114889

Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology

Department of Bioengineering, Tatung University, No 40, Sec. 3, Zhongshan N. Rd., Taipei 104, Taiwan
* Author to whom correspondence should be addressed.
Received: 31 August 2012 / Revised: 23 October 2012 / Accepted: 8 November 2012 / Published: 13 November 2012
(This article belongs to the Special Issue Enzyme Optimization and Immobilization)
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Response surface methodology (RSM) was employed to optimize the extracellular lipase production by Burkholderia sp. HL-10. Preliminary tests showed that olive oil, tryptone and Tween-80 exhibited significant effects on the lipase production. The optimum concentrations of these three components were determined using a faced-centered central composite design (FCCCD). The analysis of variance revealed that the established model was significant (p < 0.01). The optimized medium containing 0.65% olive oil (v/v), 2.42% tryptone (w/v) and 0.15% Tween-80 (v/v) resulted in a maximum activity of 122.3 U/mL, about three fold higher than that in basal medium. Approximately 99% of validity of the predicted value was achieved.
Keywords: lipase; Burkholderia sp.; optimization; response surface methodology lipase; Burkholderia sp.; optimization; response surface methodology
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Lo, C.-F.; Yu, C.-Y.; Kuan, I.-C.; Lee, S.-L. Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology. Int. J. Mol. Sci. 2012, 13, 14889-14897.

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