Isolation and Purification of a Novel Deca-Antifungal Peptide from Potato (Solanum tuberosum L. cv. Jopung) Against Candida albicans
Abstract
:1. Introduction
2. Results and Discussion
2.1. Purification of the Antifungal Peptide, Potide-J
2.2. Antifungal and Non-hemolytic Effects of Potide-J
2.3. Protein Identification
3. Experimental Section
3.1. Potato Tubers
3.2. Step I: Preparation of AFP-J
3.3. Step II: Preparation of Potide-J
3.4. Assay for Antifungal Activity
3.5. Visualize on Agar Plate of Synthetic Peptides
3.6. N-terminal Amino Acid Sequencing
3.7. Mass Spectrometry
3.8. Peptide Synthesis and Purification
4. Conclusions
Acknowledgements
References and Notes
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Fraction | Yield (mg) |
---|---|
Potato tuber | 200,000 |
Protein Extraction Buffer | 85.42 |
Gel filtration | 15.3 |
FPLC | 7.86 |
First C18-HPLC | 1.56 |
Second C18-HPLC | 0.05 |
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
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Lee, J.-K.; Gopal, R.; Seo, C.H.; Cheong, H.; Park, Y. Isolation and Purification of a Novel Deca-Antifungal Peptide from Potato (Solanum tuberosum L. cv. Jopung) Against Candida albicans. Int. J. Mol. Sci. 2012, 13, 4021-4032. https://doi.org/10.3390/ijms13044021
Lee J-K, Gopal R, Seo CH, Cheong H, Park Y. Isolation and Purification of a Novel Deca-Antifungal Peptide from Potato (Solanum tuberosum L. cv. Jopung) Against Candida albicans. International Journal of Molecular Sciences. 2012; 13(4):4021-4032. https://doi.org/10.3390/ijms13044021
Chicago/Turabian StyleLee, Jong-Kook, Ramamourthy Gopal, Chang Ho Seo, Hyeonsook Cheong, and Yoonkyung Park. 2012. "Isolation and Purification of a Novel Deca-Antifungal Peptide from Potato (Solanum tuberosum L. cv. Jopung) Against Candida albicans" International Journal of Molecular Sciences 13, no. 4: 4021-4032. https://doi.org/10.3390/ijms13044021