A Specific Oligodeoxynucleotide Promotes the Differentiation of Osteoblasts via ERK and p38 MAPK Pathways
Abstract
:1. Introduction
2. Results and Discussion
2.1. Internalization Analysis of ODNs
2.2. Effects of ODN MT01 on MAPK Signaling Proteins ERK1/2 and p38
2.3. Effects of ERK and p38 Inhibitors on Up-Regulation of ALP Activity Induced by ODN MT01
2.4. Effect of ERK and p38 Inhibitors on Runx2 Expression in Response to ODN MT01
2.5. Effects of ERK and p38 Inhibitors on the Expression of Osteocalcin and Type I Collagen
3. Experimental Section
3.1. Materials
3.2. Cell Culture
3.3. Fluorescent Labeling of MT01
3.4. Western Blot Analysis
3.5. ALP Activity Assay
3.6. Real-Time PCR
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
References
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Hou, X.; Shen, Y.; Zhang, C.; Zhang, L.; Qin, Y.; Yu, Y.; Wang, L.; Sun, X. A Specific Oligodeoxynucleotide Promotes the Differentiation of Osteoblasts via ERK and p38 MAPK Pathways. Int. J. Mol. Sci. 2012, 13, 7902-7914. https://doi.org/10.3390/ijms13077902
Hou X, Shen Y, Zhang C, Zhang L, Qin Y, Yu Y, Wang L, Sun X. A Specific Oligodeoxynucleotide Promotes the Differentiation of Osteoblasts via ERK and p38 MAPK Pathways. International Journal of Molecular Sciences. 2012; 13(7):7902-7914. https://doi.org/10.3390/ijms13077902
Chicago/Turabian StyleHou, Xu, Yuqin Shen, Chao Zhang, Liru Zhang, Yanyan Qin, Yongli Yu, Liying Wang, and Xinhua Sun. 2012. "A Specific Oligodeoxynucleotide Promotes the Differentiation of Osteoblasts via ERK and p38 MAPK Pathways" International Journal of Molecular Sciences 13, no. 7: 7902-7914. https://doi.org/10.3390/ijms13077902