Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation
AbstractHelicobacter pylori (H. pylori), the human stomach pathogen, lives on the inner surface of the stomach and causes chronic gastritis, peptic ulcer, and gastric cancer. Plasma membrane repair response is a matter of life and death for human cells against physical and biological damage. We here test the hypothesis that H. pylori also causes plasma membrane disruption injury, and that not only a membrane repair response but also a cell proliferation response are thereby activated. Vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA) have been considered to be major H. pylori virulence factors. Gastric cancer cells were infected with H. pylori wild type (vacA+/cagA+), single mutant (ΔvacA or ΔcagA) or double mutant (ΔvacA/ΔcagA) strains and plasma membrane disruption events and consequent activation of membrane repair components monitored. H. pylori disrupts the host cell plasma membrane, allowing localized dye and extracellular Ca2+ influx. Ca2+-triggered members of the annexin family, A1 and A4, translocate, in response to injury, to the plasma membrane, and cell surface expression of an exocytotic maker of repair, LAMP-2, increases. Additional forms of plasma membrane disruption, unrelated to H. pylori exposure, also promote host cell proliferation. We propose that H. pylori activation of a plasma membrane repair is pro-proliferative. This study might therefore provide new insight into potential mechanisms of H. pylori-induced gastric carcinogenesis.
- Supplementary File 1:
PDF-Document (PDF, 315 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Lin, L.-L.; Huang, H.-C.; Ogihara, S.; Wang, J.-T.; Wu, M.-C.; McNeil, P.L.; Chen, C.-N.; Juan, H.-F. Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation. Int. J. Mol. Sci. 2012, 13, 10176-10192.
Lin L-L, Huang H-C, Ogihara S, Wang J-T, Wu M-C, McNeil PL, Chen C-N, Juan H-F. Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation. International Journal of Molecular Sciences. 2012; 13(8):10176-10192.Chicago/Turabian Style
Lin, Li-Ling; Huang, Hsuan-Cheng; Ogihara, Satoshi; Wang, Jin-Town; Wu, Meng-Chuan; McNeil, Paul L.; Chen, Chiung-Nien; Juan, Hsueh-Fen. 2012. "Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation." Int. J. Mol. Sci. 13, no. 8: 10176-10192.