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Int. J. Mol. Sci. 2013, 14(2), 3946-3960; doi:10.3390/ijms14023946

Evidence for the Involvement of RhoA Signaling in the Ethanol-Induced Increase in Intestinal Epithelial Barrier Permeability

Department of Gastroenterology, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Shenyang 110001, China
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Received: 4 January 2013 / Revised: 19 January 2013 / Accepted: 24 January 2013 / Published: 18 February 2013
(This article belongs to the Special Issue Signalling Molecules and Signal Transduction in Cells)
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Abstract

In this work, we investigated the potential role of the small G protein RhoA in ethanol-induced tight junction (TJ) protein disassembly and increased intestinal epithelial barrier (IEB) permeability. Our study used Caco-2 cells as an in vitro IEB model and RhoA short hairpin RNA (shRNA) interference to establish whether RhoA plays a role in ethanol-induced TJ opening. RhoA shRNA interference partially inhibited epithelial leakage and restored normal transepithelial electrical resistance (TEER) values in the IEB. Moreover, RhoA shRNA interference prevented a shift in occludin distribution from insoluble to soluble fractions. Additionally, RhoA shRNA interference inhibited the ethanol-induced expression of zonula occludens-1 (ZO-1). Finally, RhoA shRNA interference inhibited an ethanol-induced increase in RhoA activity. The contributions of RhoA to an ethanol-induced increase in IEB permeability are associated with TJ disassembly.
Keywords: RhoA; ethanol; IEB; tight junction RhoA; ethanol; IEB; tight junction
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MDPI and ACS Style

Tong, J.; Wang, Y.; Chang, B.; Zhang, D.; Wang, B. Evidence for the Involvement of RhoA Signaling in the Ethanol-Induced Increase in Intestinal Epithelial Barrier Permeability. Int. J. Mol. Sci. 2013, 14, 3946-3960.

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