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Int. J. Mol. Sci. 2013, 14(4), 7506-7514; doi:10.3390/ijms14047506
Article

Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides

1,2
,
1,2,*  and 3,*
1 State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China 2 Institute for Technology Research and Innovation of Sun Yat-sen University, Zhuhai 519000, China 3 CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
* Authors to whom correspondence should be addressed.
Received: 14 January 2013 / Revised: 3 March 2013 / Accepted: 26 March 2013 / Published: 8 April 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

CVN (cyanovirin-N) is an anti-HIV protein. CVNH (cyanovirin-N homology) represents its homology. In a previous study, we first reported the full-length sequences of the CVNH gene cloned from Ceratopteris thalictroides. Based on the finding, the coding sequence of CtCVNH was optimized in the study, and then a pET prokaryotic expression vector was constructed. The purification and identification of CtCVNH protein were investigated, as well. SDS-PAGE analysis indicated that a 31 kDa protein was overexpressed and mainly accumulated in the soluble fraction. Only a single protein was obtained after the Ni- nitrilotriacetic acid (NTA) affinity chromatography. The purified protein was identified to be the recombinant CtCVNH by both Western blot and peptide mass fingerprinting analysis.
Keywords: Ceratopteris thalictroides; CtCVNH; anti-HIV protein; prokaryotic expression Ceratopteris thalictroides; CtCVNH; anti-HIV protein; prokaryotic expression
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Sun, J.; Su, Y.; Wang, T. Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides. Int. J. Mol. Sci. 2013, 14, 7506-7514.

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