Next Article in Journal
Monocyte Chemotactic Protein-1 Promotes the Myocardial Homing of Mesenchymal Stem Cells in Dilated Cardiomyopathy
Next Article in Special Issue
Crosstalk between DnaA Protein, the Initiator of Escherichia coli Chromosomal Replication, and Acidic Phospholipids Present in Bacterial Membranes
Previous Article in Journal
Role of Homeodomain Leucine Zipper (HD-Zip) IV Transcription Factors in Plant Development and Plant Protection from Deleterious Environmental Factors
Previous Article in Special Issue
P4 ATPases: Flippases in Health and Disease
Article Menu

Export Article

Open AccessReview
Int. J. Mol. Sci. 2013, 14(4), 8148-8163; doi:10.3390/ijms14048148

Phospholipid Membrane Protection by Sugar Molecules during Dehydration—Insights into Molecular Mechanisms Using Scattering Techniques

1
Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC NSW 2232, Australia
2
Research School of Biological Sciences, the Australian National University, Canberra, ACT 0200, Australia
3
Department of Biology, The University of South Dakota, Vermillion, SD 57069, USA
4
School of Applied Sciences, RMIT University, Melbourne, VIC 3001, Australia
*
Author to whom correspondence should be addressed.
Received: 14 December 2012 / Revised: 3 April 2013 / Accepted: 9 April 2013 / Published: 12 April 2013
(This article belongs to the Special Issue Phospholipids: Molecular Sciences 2012)
View Full-Text   |   Download PDF [1730 KB, uploaded 19 June 2014]   |  

Abstract

Scattering techniques have played a key role in our understanding of the structure and function of phospholipid membranes. These techniques have been applied widely to study how different molecules (e.g., cholesterol) can affect phospholipid membrane structure. However, there has been much less attention paid to the effects of molecules that remain in the aqueous phase. One important example is the role played by small solutes, particularly sugars, in protecting phospholipid membranes during drying or slow freezing. In this paper, we present new results and a general methodology, which illustrate how contrast variation small angle neutron scattering (SANS) and synchrotron-based X-ray scattering (small angle (SAXS) and wide angle (WAXS)) can be used to quantitatively understand the interactions between solutes and phospholipids. Specifically, we show the assignment of lipid phases with synchrotron SAXS and explain how SANS reveals the exclusion of sugars from the aqueous region in the particular example of hexagonal II phases formed by phospholipids. View Full-Text
Keywords: cryobiology; anhydrobiology; X-ray scattering; contrast variation small angle neutron scattering; membranes; phospholipids; sugars cryobiology; anhydrobiology; X-ray scattering; contrast variation small angle neutron scattering; membranes; phospholipids; sugars
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Garvey, C.J.; Lenné, T.; Koster, K.L.; Kent, B.; Bryant, G. Phospholipid Membrane Protection by Sugar Molecules during Dehydration—Insights into Molecular Mechanisms Using Scattering Techniques. Int. J. Mol. Sci. 2013, 14, 8148-8163.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top