Detection of Promyelocytic Leukemia/Retinoic Acid Receptor α (PML/RARα) Fusion Gene with Functionalized Graphene Oxide
Abstract
:1. Introduction
2. Results and Discussion
2.1. Preparation of the Functionalized GO and Its Stability in Biological Solutions
2.2. The Single-Stranded DNA Fluorescence Quenched by the Functionalized GO and Recovered by the Target Molecule in Vitro without Cells
2.3. The Single-Stranded DNA Fluorescence Quenched by the Functionalized GO and Recovered by the Target Molecule with Cells
2.3.1. The Results with Laser Confocal Microscopy and the Analysis of Results
2.3.2. The Results with Flow Cytometry and the Analysis of Results
3. Materials and Methods
3.1. Preparation of GO
3.2. Functionalization of GO with Polyethylene Glycol
3.3. Design of Probe
3.4. Fluorescence Quenching and Hybridization Assays
3.5. Cell Culture
3.6. Cell Pretreatment
3.7. The Incubation Medium and the Cells Were Incubated
4. Conclusions
Acknowledgments
Conflict of Interest
References
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Reagent | NB4 (negative group) | NB4 (positive group) | K562 (negative group) | K562 (positive group) |
---|---|---|---|---|
GO | 0.04 mg/mL | 0.04 mg/mL | 0.04 mg/mL | 0.04 mg/mL |
Probe | - | 200 nM | - | 200 nM |
Tris-Hcl buffer | 20 mM | 20 mM | 20 mM | 20 mM |
Packet | Interventions | PML/RARα fusion gene positive cells (%) | F | P | ||
---|---|---|---|---|---|---|
The negative group | Tris-Hcl buffer | The functionalized GO | - | 1.20 ± 0.20 | 134.384 | 0.000 |
The positive group | Tris-Hcl buffer | The functionalized GO | The FITC-labeled single-stranded DNA | 56.00 ± 8.19 |
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Li, R.; Tan, Y.; Chen, X.; Ren, F.; Zhang, Y.; Xu, Z.; Wang, H. Detection of Promyelocytic Leukemia/Retinoic Acid Receptor α (PML/RARα) Fusion Gene with Functionalized Graphene Oxide. Int. J. Mol. Sci. 2013, 14, 12863-12872. https://doi.org/10.3390/ijms140612863
Li R, Tan Y, Chen X, Ren F, Zhang Y, Xu Z, Wang H. Detection of Promyelocytic Leukemia/Retinoic Acid Receptor α (PML/RARα) Fusion Gene with Functionalized Graphene Oxide. International Journal of Molecular Sciences. 2013; 14(6):12863-12872. https://doi.org/10.3390/ijms140612863
Chicago/Turabian StyleLi, Ran, Yanhong Tan, Xiuhua Chen, Fanggang Ren, Yaofang Zhang, Zhifang Xu, and Hongwei Wang. 2013. "Detection of Promyelocytic Leukemia/Retinoic Acid Receptor α (PML/RARα) Fusion Gene with Functionalized Graphene Oxide" International Journal of Molecular Sciences 14, no. 6: 12863-12872. https://doi.org/10.3390/ijms140612863