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Int. J. Mol. Sci. 2013, 14(6), 12863-12872; doi:10.3390/ijms140612863
Article

Detection of Promyelocytic Leukemia/Retinoic Acid Receptor α (PML/RARα) Fusion Gene with Functionalized Graphene Oxide

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Department of Hematology, the Second Hospital of Shanxi Medical University, Taiyuan 030001, China
* Author to whom correspondence should be addressed.
Received: 25 March 2013 / Revised: 30 May 2013 / Accepted: 9 June 2013 / Published: 20 June 2013
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Abstract

An attempt was made to use functionalized graphene oxide (GO) to detect the Promyelocytic leukemia/Retinoic acid receptor α fusion gene (PML/RARα fusion gene), a marker gene of acute promyelocytic leukemia. The functionalized GO was prepared by chemical exfoliation method, followed by a polyethylene glycol grafting. It is found that the functionalized GO can selectively adsorb the fluorescein isothiocyanate (FITC)-labeled single-stranded DNA probe and quench its fluorescence. The probe can be displaced by the PML/RARα fusion gene to restore the fluorescence, which can be detected by laser confocal microscopy and flow cytometry. These can be used to detect the presence of the PML/RARα fusion gene. This detection method is verified to be fast, simple and reliable.
Keywords: PML/RARα fusion gene; graphene oxide; fluorescein isothiocyanate (FITC); laser confocal microscopy PML/RARα fusion gene; graphene oxide; fluorescein isothiocyanate (FITC); laser confocal microscopy
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Li, R.; Tan, Y.; Chen, X.; Ren, F.; Zhang, Y.; Xu, Z.; Wang, H. Detection of Promyelocytic Leukemia/Retinoic Acid Receptor α (PML/RARα) Fusion Gene with Functionalized Graphene Oxide. Int. J. Mol. Sci. 2013, 14, 12863-12872.

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