Next Article in Journal
Computational Study on Substrate Specificity of a Novel Cysteine Protease 1 Precursor from Zea mays
Previous Article in Journal
Alternative Splicing in Plant Immunity
Int. J. Mol. Sci. 2014, 15(6), 10446-10458; doi:10.3390/ijms150610446
Article

Triterpenoid Saponins from Stauntonia chinensis Ameliorate Insulin Resistance via the AMP-Activated Protein Kinase and IR/IRS-1/PI3K/Akt Pathways in Insulin-Resistant HepG2 Cells

1,†
,
1,†
,
1
,
1
,
2
 and
1,*
1 Laboratory for Natural Products Chemistry, College of Pharmacy, South Central University for Nationalities, Wuhan 430074, China 2 College of Chemistry and Material Sciences, South Central University for Nationalities, Wuhan 430074, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 11 April 2014 / Revised: 20 May 2014 / Accepted: 26 May 2014 / Published: 10 June 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [1562 KB, uploaded 19 June 2014]   |   Browse Figures
SciFeed

Abstract

Inflammation and oxidative stress play crucial roles in the etiology of type 2 diabetes mellitus. In this study, we examined the anti-diabetic effects of triterpenoid saponins extracted from Stauntonia chinensis on stimulating glucose uptake by insulin-resistant human HepG2 cells. The results showed that saponin 6 significantly increased glucose uptake and glucose catabolism. Saponin 6 also enhanced the phosphorylation of AMP-activated protein kinase (AMPK) and activated the insulin receptor (IR)/insulin receptor substrate-1 (IRS-1)/phosphoinositide 3-kinase (PI3K)/Akt pathway. Therefore, our results suggest that saponins from S. chinensis improve glucose uptake and catabolism in hepatic cells by stimulating the AMPK and the IR/IRS-1/PI3K/Akt signaling pathways. The results also imply that saponins from S. chinensis can enhance glucose uptake and insulin sensitivity, representing a promising treatment for type 2 diabetes mellitus.
Keywords: Stauntonia chinensis; insulin-resistance; AMPK; IR/IRS-1/PI3K/Akt signaling pathway; HepG2 cells Stauntonia chinensis; insulin-resistance; AMPK; IR/IRS-1/PI3K/Akt signaling pathway; HepG2 cells
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Hu, X.; Wang, S.; Xu, J.; Wang, D.-B.; Chen, Y.; Yang, G.-Z. Triterpenoid Saponins from Stauntonia chinensis Ameliorate Insulin Resistance via the AMP-Activated Protein Kinase and IR/IRS-1/PI3K/Akt Pathways in Insulin-Resistant HepG2 Cells. Int. J. Mol. Sci. 2014, 15, 10446-10458.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert