ESTs Analysis of Putative Genes Engaged in Polyporus umbellatus Sclerotial Development
Abstract
:1. Introduction
2. Results and Discussion
2.1. Morphological Identification
2.2. EST (Expressed Sequence Tag) Assembly
2.3. Functional Characterization of EST Data Set
2.4. Transcription Expression Patterns of Candidate Genes through RT-PCR Assay
3. Experimental Section
3.1. Sclerotial Material Preparation
3.2. RNA and mRNA Isolation
3.3. Suppression Subtractive Hybridization (SSH)
3.4. cDNA Sequencing and EST Analyses
3.5. Real-Time qPCR Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Song, C.; Liu, M.; Xing, Y.; Guo, S. ESTs Analysis of Putative Genes Engaged in Polyporus umbellatus Sclerotial Development. Int. J. Mol. Sci. 2014, 15, 15951-15962. https://doi.org/10.3390/ijms150915951
Song C, Liu M, Xing Y, Guo S. ESTs Analysis of Putative Genes Engaged in Polyporus umbellatus Sclerotial Development. International Journal of Molecular Sciences. 2014; 15(9):15951-15962. https://doi.org/10.3390/ijms150915951
Chicago/Turabian StyleSong, Chao, Mengmeng Liu, Yongmei Xing, and Shunxing Guo. 2014. "ESTs Analysis of Putative Genes Engaged in Polyporus umbellatus Sclerotial Development" International Journal of Molecular Sciences 15, no. 9: 15951-15962. https://doi.org/10.3390/ijms150915951