RETRACTED: Microenvironment Stimuli HGF and Hypoxia Differently Affected miR-125b and Ets-1 Function with Opposite Effects on the Invasiveness of Bone Metastatic Cells: A Comparison with Breast Carcinoma Cells
Abstract
:1. Introduction
2. Results
2.1. Different Effects of Stimuli of Bone Metastasis Microenvironment on the Expression of miR-125b, the Ets1 Protein Levels and Invasiveness in MDA-MB231 and 1833 Cells
2.2. Reciprocal Regulation of Ets-1 and HIF-1α Transactivating Activities in 1833 Cells
2.3. Regulation of Endothelin-1 Expression by Microenvironment Stimuli in 1833 Cells
3. Discussion
4. Materials and Methods
4.1. Cell Cultures and miR-125b Transfection
4.2. Western Blot Analysis
4.3. Purification of Small RNA Species (miRNAs), and Quantification of miR-125b
4.4. Matrigel Invasion Assay
4.5. Constructs and Cell Transfections
4.6. EMSA Analysis
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
HGF | hepatocyte growth factor |
HIF | hypoxia inducible factor |
HRE | HIF-1 responsive element |
MET | mesenchymal–epithelial transition |
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Matteucci, E.; Maroni, P.; Nicassio, F.; Ghini, F.; Bendinelli, P.; Desiderio, M.A. RETRACTED: Microenvironment Stimuli HGF and Hypoxia Differently Affected miR-125b and Ets-1 Function with Opposite Effects on the Invasiveness of Bone Metastatic Cells: A Comparison with Breast Carcinoma Cells. Int. J. Mol. Sci. 2018, 19, 258. https://doi.org/10.3390/ijms19010258
Matteucci E, Maroni P, Nicassio F, Ghini F, Bendinelli P, Desiderio MA. RETRACTED: Microenvironment Stimuli HGF and Hypoxia Differently Affected miR-125b and Ets-1 Function with Opposite Effects on the Invasiveness of Bone Metastatic Cells: A Comparison with Breast Carcinoma Cells. International Journal of Molecular Sciences. 2018; 19(1):258. https://doi.org/10.3390/ijms19010258
Chicago/Turabian StyleMatteucci, Emanuela, Paola Maroni, Francesco Nicassio, Francesco Ghini, Paola Bendinelli, and Maria Alfonsina Desiderio. 2018. "RETRACTED: Microenvironment Stimuli HGF and Hypoxia Differently Affected miR-125b and Ets-1 Function with Opposite Effects on the Invasiveness of Bone Metastatic Cells: A Comparison with Breast Carcinoma Cells" International Journal of Molecular Sciences 19, no. 1: 258. https://doi.org/10.3390/ijms19010258
APA StyleMatteucci, E., Maroni, P., Nicassio, F., Ghini, F., Bendinelli, P., & Desiderio, M. A. (2018). RETRACTED: Microenvironment Stimuli HGF and Hypoxia Differently Affected miR-125b and Ets-1 Function with Opposite Effects on the Invasiveness of Bone Metastatic Cells: A Comparison with Breast Carcinoma Cells. International Journal of Molecular Sciences, 19(1), 258. https://doi.org/10.3390/ijms19010258