Deficiency of Invariant Natural Killer T Cells Does Not Protect Against Obesity but Exacerbates Atherosclerosis in Ldlr−/− Mice
Abstract
:1. Introduction
2. Results
2.1. Natural Killer T (NKT) Cell Deficiency Is Associated with Weight Gain in Ldlr−/− Mice on a High Fat, High Sucrose Cholesterol Enriched (HFHSC) Diet
2.2. Obesity-Induced Metabolic Abnormalities Are Observed in the Presence and Absence of NKT Cells
2.3. Adipose Tissue Inflammation Is Worsened in Obese Ja18−/−Ldlr−/− Mice Devoid of iNKT Cells
2.4. Hepatic Steatosis Is Observed in Obese Mice in the Presence and Absence of iNKT Cells
2.5. Aortic Atherosclerosis Is Increased in Ja18−/−Ldlr−/− Mice
3. Discussion
4. Materials and Methods
4.1. Animals and Diets
4.2. Analytical Procedures
4.3. Real-Time Quantitative PCR
4.4. Histology, Immunohistochemistry, and Atherosclerosis Quantification
4.5. Statistics
Acknowledgments
Authors Contribution
Conflicts of Interest
Abbreviations
FPLC | fast performance liquid chromatography |
HFHSC | high fat, high sucrose, cholesterol |
iNKT | invariant Natural Killer T |
Ldlr | Low density lipoprotein receptor |
SAA | serum amyloid A |
TCR | T cell receptor |
Treg | T regulatory |
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Subramanian, S.; Goodspeed, L.; Wang, S.; Ding, Y.; O’Brien, K.D.; Getz, G.S.; Chait, A.; Reardon, C.A. Deficiency of Invariant Natural Killer T Cells Does Not Protect Against Obesity but Exacerbates Atherosclerosis in Ldlr−/− Mice. Int. J. Mol. Sci. 2018, 19, 510. https://doi.org/10.3390/ijms19020510
Subramanian S, Goodspeed L, Wang S, Ding Y, O’Brien KD, Getz GS, Chait A, Reardon CA. Deficiency of Invariant Natural Killer T Cells Does Not Protect Against Obesity but Exacerbates Atherosclerosis in Ldlr−/− Mice. International Journal of Molecular Sciences. 2018; 19(2):510. https://doi.org/10.3390/ijms19020510
Chicago/Turabian StyleSubramanian, Savitha, Leela Goodspeed, Shari Wang, Yilei Ding, Kevin D. O’Brien, Godfrey S. Getz, Alan Chait, and Catherine A. Reardon. 2018. "Deficiency of Invariant Natural Killer T Cells Does Not Protect Against Obesity but Exacerbates Atherosclerosis in Ldlr−/− Mice" International Journal of Molecular Sciences 19, no. 2: 510. https://doi.org/10.3390/ijms19020510
APA StyleSubramanian, S., Goodspeed, L., Wang, S., Ding, Y., O’Brien, K. D., Getz, G. S., Chait, A., & Reardon, C. A. (2018). Deficiency of Invariant Natural Killer T Cells Does Not Protect Against Obesity but Exacerbates Atherosclerosis in Ldlr−/− Mice. International Journal of Molecular Sciences, 19(2), 510. https://doi.org/10.3390/ijms19020510