Complementary Regulation of BfmRS Two-Component and AbaIR Quorum Sensing Systems to Express Virulence-Associated Genes in Acinetobacter baumannii
Abstract
:1. Introduction
2. Results
2.1. Effect of BfmRS on Bacterial Growth and Capsule Production
2.2. Effect of BfmRS on the Surface Motility, Twitching Motility, Biofilm Formation, and Pellicle Formation
2.3. Regulation of Surface-Motility-, Twitching-Motility-, and Biofilm-Associated Genes by BfmRS
2.4. Effect of BfmRS on the Production of Autoinducers
2.5. Effect of AbaR on the Expression of Biofilm- and Surface-Motility-Associated Genes
3. Discussion
4. Materials and Methods
4.1. Bacteria, Plasmids, Culture Media, and Growth Conditions
4.2. Construction of the ΔbfmR Mutant and bfmR-Complemented Strains
4.3. Bacterial Growth Studies
4.4. RNA Isolation and Gene Expression Assay Using qPCR
4.5. Surface Motility Assay
4.6. Biofilm and Pellicle Formation Assays
4.7. Bioassay for the Detection of Autoinducers
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, H.-J.; Kim, N.-Y.; Ko, S.-Y.; Park, S.-Y.; Oh, M.-H.; Shin, M.-S.; Lee, Y.-C.; Lee, J.-C. Complementary Regulation of BfmRS Two-Component and AbaIR Quorum Sensing Systems to Express Virulence-Associated Genes in Acinetobacter baumannii. Int. J. Mol. Sci. 2022, 23, 13136. https://doi.org/10.3390/ijms232113136
Kim H-J, Kim N-Y, Ko S-Y, Park S-Y, Oh M-H, Shin M-S, Lee Y-C, Lee J-C. Complementary Regulation of BfmRS Two-Component and AbaIR Quorum Sensing Systems to Express Virulence-Associated Genes in Acinetobacter baumannii. International Journal of Molecular Sciences. 2022; 23(21):13136. https://doi.org/10.3390/ijms232113136
Chicago/Turabian StyleKim, Hyo-Jeong, Na-Yeong Kim, Seo-Yeon Ko, Seong-Yong Park, Man-Hwan Oh, Min-Sang Shin, Yoo-Chul Lee, and Je-Chul Lee. 2022. "Complementary Regulation of BfmRS Two-Component and AbaIR Quorum Sensing Systems to Express Virulence-Associated Genes in Acinetobacter baumannii" International Journal of Molecular Sciences 23, no. 21: 13136. https://doi.org/10.3390/ijms232113136