INO80 Is Required for the Cell Cycle Control, Survival, and Differentiation of Mouse ESCs by Transcriptional Regulation
Abstract
:1. Introduction
2. Results
2.1. Ino80 Loss in ESCs Causes Aberrant Cell Cycle and Cell Death
2.2. Ino80 Loss Alters Expression of Genes Related to Cell Cycle
2.3. Abnormal Cell Cycle Profile Is Persistent in Differentiating ESCs
2.4. Ino80 Loss Impairs Expression of Genes Involved in Differentiation of Mesodermal Lineages
2.5. Conserved and Unique Alteration of Gene Expression between Undifferentiated and Differentiating iKO ESCs
3. Discussion
4. Materials and Methods
4.1. Animals and Generation of Ino80 iKO ESCs
4.2. ESC Differentiation
4.3. PCR Genotyping
4.4. Analyses of Cell Proliferation, Cell Cycle, and Apoptosis
4.5. Immunofluorescence
4.6. Quantitative RT-PCR Analysis
4.7. RNA-seq and ChIP-seq Analysis
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yoo, S.; Lee, E.J.; Thang, N.X.; La, H.; Lee, H.; Park, C.; Han, D.W.; Uhm, S.J.; Song, H.; Do, J.T.; et al. INO80 Is Required for the Cell Cycle Control, Survival, and Differentiation of Mouse ESCs by Transcriptional Regulation. Int. J. Mol. Sci. 2022, 23, 15402. https://doi.org/10.3390/ijms232315402
Yoo S, Lee EJ, Thang NX, La H, Lee H, Park C, Han DW, Uhm SJ, Song H, Do JT, et al. INO80 Is Required for the Cell Cycle Control, Survival, and Differentiation of Mouse ESCs by Transcriptional Regulation. International Journal of Molecular Sciences. 2022; 23(23):15402. https://doi.org/10.3390/ijms232315402
Chicago/Turabian StyleYoo, Seonho, Eun Joo Lee, Nguyen Xuan Thang, Hyeonwoo La, Hyeonji Lee, Chanhyeok Park, Dong Wook Han, Sang Jun Uhm, Hyuk Song, Jeong Tae Do, and et al. 2022. "INO80 Is Required for the Cell Cycle Control, Survival, and Differentiation of Mouse ESCs by Transcriptional Regulation" International Journal of Molecular Sciences 23, no. 23: 15402. https://doi.org/10.3390/ijms232315402