MicroRNA-152-3p and MicroRNA-196a-5p Are Downregulated When Müller Cells Are Promoted by Components of the Internal Limiting Membrane: Implications for Macular Hole Healing
Abstract
:1. Introduction
2. Results
2.1. Effect of ILM Components in Müller Cell Migration
2.2. MiRNA Profiling of Müller Cell Stimulated by ILM Components
2.3. Transfection of MiRNA Mimics and Inhibitors
2.4. Prediction of Target Genes for Selected MiRNAs
3. Discussion
4. Materials and Methods
4.1. Cell Line
4.2. Cell Migration Assay
4.3. RNA Purification and MiRNA Profiling
4.4. Transfection of MiRNA Mimics and Inhibitors Followed by Migration Assay
4.5. Cell Proliferation Assay
4.6. Target Gene Prediction and Gene Ontology Enrichment Analysis
4.7. Statistical Analyses
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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MicroRNA | Sequence |
---|---|
rno-miR-196a-5p | UAGGUAGUUUCAUGUUGUUGGG |
rno-miR-152-3p | UCAGUGCAUGACAGAACUUGG |
rno-miR-132-3p | UAACAGUCUACAGCCAUGGUCG |
rno-miR-146b-5p | UGAGAACUGAAUUCCAUAGGCUGU |
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Chou, H.-D.; Shiah, S.-G.; Chuang, L.-H.; Wu, W.-C.; Hwang, Y.-S.; Chen, K.-J.; Kang, E.Y.-C.; Yeung, L.; Nien, C.-Y.; Lai, C.-C. MicroRNA-152-3p and MicroRNA-196a-5p Are Downregulated When Müller Cells Are Promoted by Components of the Internal Limiting Membrane: Implications for Macular Hole Healing. Int. J. Mol. Sci. 2023, 24, 17188. https://doi.org/10.3390/ijms242417188
Chou H-D, Shiah S-G, Chuang L-H, Wu W-C, Hwang Y-S, Chen K-J, Kang EY-C, Yeung L, Nien C-Y, Lai C-C. MicroRNA-152-3p and MicroRNA-196a-5p Are Downregulated When Müller Cells Are Promoted by Components of the Internal Limiting Membrane: Implications for Macular Hole Healing. International Journal of Molecular Sciences. 2023; 24(24):17188. https://doi.org/10.3390/ijms242417188
Chicago/Turabian StyleChou, Hung-Da, Shine-Gwo Shiah, Lan-Hsin Chuang, Wei-Chi Wu, Yih-Shiou Hwang, Kuan-Jen Chen, Eugene Yu-Chuan Kang, Ling Yeung, Chung-Yi Nien, and Chi-Chun Lai. 2023. "MicroRNA-152-3p and MicroRNA-196a-5p Are Downregulated When Müller Cells Are Promoted by Components of the Internal Limiting Membrane: Implications for Macular Hole Healing" International Journal of Molecular Sciences 24, no. 24: 17188. https://doi.org/10.3390/ijms242417188