Isolation of Pro-Osteogenic Compounds from Euptelea polyandra That Reciprocally Regulate Osteoblast and Osteoclast Differentiation
Abstract
:1. Introduction
2. Results
2.1. Isolation of Osteoblast Differentiation-Promoting Compounds from E. polyandra
2.2. Effects of the Isolated Compounds on RANKL-Induced Osteoclastogenesis
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Extraction, Solvent Fractionation, and Column Chromatography
4.3. Osteoblast Differentiation
4.4. ALP Activity
4.5. Cell Viability Analysis
4.6. Mineralization Analysis
4.7. Osteoclast Differentiation
4.8. TRAP Activity Assay and Staining
4.9. RNA Extraction and Quantitative PCR (qPCR)
4.10. Instrumental Analysis
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Suzuki, R.; Shirataki, Y.; Tomomura, A.; Bandow, K.; Sakagami, H.; Tomomura, M. Isolation of Pro-Osteogenic Compounds from Euptelea polyandra That Reciprocally Regulate Osteoblast and Osteoclast Differentiation. Int. J. Mol. Sci. 2023, 24, 17479. https://doi.org/10.3390/ijms242417479
Suzuki R, Shirataki Y, Tomomura A, Bandow K, Sakagami H, Tomomura M. Isolation of Pro-Osteogenic Compounds from Euptelea polyandra That Reciprocally Regulate Osteoblast and Osteoclast Differentiation. International Journal of Molecular Sciences. 2023; 24(24):17479. https://doi.org/10.3390/ijms242417479
Chicago/Turabian StyleSuzuki, Ryuichiro, Yoshiaki Shirataki, Akito Tomomura, Kenjiro Bandow, Hiroshi Sakagami, and Mineko Tomomura. 2023. "Isolation of Pro-Osteogenic Compounds from Euptelea polyandra That Reciprocally Regulate Osteoblast and Osteoclast Differentiation" International Journal of Molecular Sciences 24, no. 24: 17479. https://doi.org/10.3390/ijms242417479