Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium
Abstract
:1. Introduction
2. Results
2.1. Surface Characterization
2.2. Evaluation of Protein Adsorption on the Plasma and Nano-Modified Titanium Surface
2.3. Effects of Plasma and Nano-Modified Titanium Surfaces on Cell Adhesion and Morphology in RBMCs
2.4. In Vitro Argon Plasma Treatment-Induced Bone Differentiation on the Nano-Modified Titanium Surface
2.5. Cell Intracellular Reactive Oxygen Species (ROS) Level of RBMCs on Nano-Modified Titanium Surface
2.6. Simulated Body Fluid Immersion Experiment
2.7. Evaluation of the Amount of New Bone Formation in the Tissue Surrounding the Nano-Modified Titanium Implant Placement In Vivo
3. Discussion
4. Materials and Methods
4.1. Sample Preparation
4.2. Surface Characterization
4.3. Protein Adsorption
4.4. Cell Culture
4.5. Cell Adhesion
4.6. Quantitative Reverse Transcription Polymerase Chain Reaction (Qrt-Pcr), Alp Activity, Dna Content, and Mineralization Determination
4.7. Intracellular ROS Levels in Rbmcs
4.8. Simulated Body Fluid (SBF) Test
4.9. Animal Model and Surgical Procedures
4.10. In Vivo Argon Plasma-Induced Bone Differentiation on the TNS-Modified Titanium Surface
4.11. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hayashi, R.; Takao, S.; Komasa, S.; Sekino, T.; Kusumoto, T.; Maekawa, K. Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium. Int. J. Mol. Sci. 2024, 25, 149. https://doi.org/10.3390/ijms25010149
Hayashi R, Takao S, Komasa S, Sekino T, Kusumoto T, Maekawa K. Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium. International Journal of Molecular Sciences. 2024; 25(1):149. https://doi.org/10.3390/ijms25010149
Chicago/Turabian StyleHayashi, Rina, Seiji Takao, Satoshi Komasa, Tohru Sekino, Tetsuji Kusumoto, and Kenji Maekawa. 2024. "Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium" International Journal of Molecular Sciences 25, no. 1: 149. https://doi.org/10.3390/ijms25010149