The
Mycobacterium sp. BRS2A-AR2 is an endophyte of the mangrove plant
Rhizophora racemosa G. Mey., which grows along the banks of the River Butre, in the Western Region of Ghana. Chemical profiling using
1H-NMR and HRESI-LC-MS of fermentation extracts produced by the
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The
Mycobacterium sp. BRS2A-AR2 is an endophyte of the mangrove plant
Rhizophora racemosa G. Mey., which grows along the banks of the River Butre, in the Western Region of Ghana. Chemical profiling using
1H-NMR and HRESI-LC-MS of fermentation extracts produced by the strain led to the isolation of the new compound, α-
d-Glucopyranosyl-(1→2)-[6-
O-(
l-tryptophanyl)-β-
d–fructofuranoside] or simply tortomycoglycoside (
1). Compound
1 is an aminoglycoside consisting of a tryptophan moiety esterified to a disaccharide made up of β-
d-fructofuranose and α-
d-glucopyranose sugars. The full structure of
1 was determined using UV, IR, 1D, 2D-NMR and HRESI-LC-MS data. When tested against
Trypanosoma brucei subsp.
brucei, the parasite responsible for Human African Trypanosomiasis in sub-Saharan Africa,
1 (IC
50 11.25 µM) was just as effective as
Coptis japonica (Thunb.) Makino. (IC
50 8.20 µM). The extract of
Coptis japonica (Thunb.) Makino. is routinely used as laboratory standard due to its powerful antitrypanosomal activity. It is possible that, compound
1 interferes with the normal uptake and metabolism of tryptophan in the
T. brucei subsp.
brucei parasite.
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