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Mar. Drugs 2017, 15(10), 304; doi:10.3390/md15100304

Sequencing and Characterization of Novel PII Signaling Protein Gene in Microalga Haematococcus pluvialis

1,2
,
2,* and 1,*
1
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
2
Algae Biotechnology Laboratory, School of Agriculture and Food Sciences, The University of Queensland, Brisbane 4072, Queensland, Australia
*
Authors to whom correspondence should be addressed.
Received: 31 August 2017 / Revised: 25 September 2017 / Accepted: 30 September 2017 / Published: 11 October 2017
(This article belongs to the Collection Marine Carotenoids)
View Full-Text   |   Download PDF [2599 KB, uploaded 11 October 2017]   |  

Abstract

The PII signaling protein is a key protein for controlling nitrogen assimilatory reactions in most organisms, but little information is reported on PII proteins of green microalga Haematococcus pluvialis. Since H. pluvialis cells can produce a large amount of astaxanthin upon nitrogen starvation, its PII protein may represent an important factor on elevated production of Haematococcus astaxanthin. This study identified and isolated the coding gene (HpGLB1) from this microalga. The full-length of HpGLB1 was 1222 bp, including 621 bp coding sequence (CDS), 103 bp 5′ untranslated region (5′ UTR), and 498 bp 3′ untranslated region (3′ UTR). The CDS could encode a protein with 206 amino acids (HpPII). Its calculated molecular weight (Mw) was 22.4 kDa and the theoretical isoelectric point was 9.53. When H. pluvialis cells were exposed to nitrogen starvation, the HpGLB1 expression was increased 2.46 times in 48 h, concomitant with the raise of astaxanthin content. This study also used phylogenetic analysis to prove that HpPII was homogeneous to the PII proteins of other green microalgae. The results formed a fundamental basis for the future study on HpPII, for its potential physiological function in Haematococcus astaxanthin biosysthesis. View Full-Text
Keywords: Haematococcus pluvialis; PII signaling protein; nitrogen starvation; gene cloning; mRNA expression Haematococcus pluvialis; PII signaling protein; nitrogen starvation; gene cloning; mRNA expression
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Ma, R.; Li, Y.; Lu, Y. Sequencing and Characterization of Novel PII Signaling Protein Gene in Microalga Haematococcus pluvialis. Mar. Drugs 2017, 15, 304.

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