Carbon Nanotubes: Solution for the Therapeutic Delivery of siRNA?
Abstract
:1. Introduction
1.1. Therapeutic Uses of Carbon Nanotubes
1.2. Toxicity
1.3. PK and Biodistribution
1.4. SWCNT Delivery of siRNA
2. Results and Discussion
2.1. Processing of SWCNT Samples for Biological Evaluation
2.2. Transfection of siRNA/SWCNT Complexes
2.3. Stability of siRNA/SWCNT Complexes
2.4. Biological Activity
2.5. Manipulating the Pharmacokinetic Properties of SWCNT
3. Experimental Section
3.1. Preparation of SWCNT Solutions and SWCNT Complexes with siRNA
3.2. siRNA/PEG/SWCNT Complex Preparation
3.2.1. Atomic Force Microscopy (AFM)
3.2.2. Dynamic Light Scattering (DLS) and Zeta Potential
3.3. In Vitro Stability of siRNA/SWCNT Complexes
3.4. In Vitro Cellular Uptake Studies
3.5. In Vivo Analyses of SWCNT and siRNA/SWCNT Complexes
3.5.1. Toxicity Evaluation of SWCNT with no siRNA Payload
3.5.2. Pharmacokinetic Analyses of siRNA/PEG/SWCNT Solutions
3.5.3. Biodistribution and Elimination
3.6. Antitumor and Toxicity Evaluation of siEGFR/siKRAS/ SWCNT Payloads
3.6.1. Preparation of siEGFR/SWCNT or siKRAS/SWCNT and siEGFR/siKRAS/SWCNT
3.6.2. Animal Treatment Schedule, Blood Draws and Tissue Harvesting
3.6.3. Tissue Analyses
4. Conclusions
Acknowledgments
References
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Kirkpatrick, D.L.; Weiss, M.; Naumov, A.; Bartholomeusz, G.; Weisman, R.B.; Gliko, O. Carbon Nanotubes: Solution for the Therapeutic Delivery of siRNA? Materials 2012, 5, 278-301. https://doi.org/10.3390/ma5020278
Kirkpatrick DL, Weiss M, Naumov A, Bartholomeusz G, Weisman RB, Gliko O. Carbon Nanotubes: Solution for the Therapeutic Delivery of siRNA? Materials. 2012; 5(2):278-301. https://doi.org/10.3390/ma5020278
Chicago/Turabian StyleKirkpatrick, D. Lynn, Michelle Weiss, Anton Naumov, Geoffrey Bartholomeusz, R. Bruce Weisman, and Olga Gliko. 2012. "Carbon Nanotubes: Solution for the Therapeutic Delivery of siRNA?" Materials 5, no. 2: 278-301. https://doi.org/10.3390/ma5020278