Dynamic Morphological Changes Induced By GM1 and Protein Interactions on the Surface of Cell-Sized Liposomes
Abstract
:1. Introduction
2. Results
2.1. Homogeneous Liposome
2.1.1. Homogeneous Liposome with Variable Concentration of GM1
2.1.2. Homogeneous Liposome with Variable Concentration of GM1 with Ctb
2.2. Heterogeneous Liposome
- a.
- 5:5 (DOPC:DPPC) So/Ld phase;
- b.
- 4:4:2 (DOPC:DPPC:Cholesterol) Lo/Ld phase;
- c.
- 2:5:3 (DOPC:DPPC:Cholesterol) Lo/Ld phase.
2.2.1. Heterogeneous Liposome with Variable Concentration of GM1
2.2.2. Heterogeneous Liposome with GM1-Ctb Interaction
3. Experimental Section
3.1. Materials
3.2. Preparation of Giant Unilamelar Vesicles (GUV)
3.3. Microscopic Observations
3.4. Induction of Osmotic Stress
4. Discussion
Acknowledgments
Conflict of Interest
References
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Dhingra, S.; Morita, M.; Yoda, T.; Vestergaard, M.C.; Hamada, T.; Takagi, M. Dynamic Morphological Changes Induced By GM1 and Protein Interactions on the Surface of Cell-Sized Liposomes. Materials 2013, 6, 2522-2533. https://doi.org/10.3390/ma6062522
Dhingra S, Morita M, Yoda T, Vestergaard MC, Hamada T, Takagi M. Dynamic Morphological Changes Induced By GM1 and Protein Interactions on the Surface of Cell-Sized Liposomes. Materials. 2013; 6(6):2522-2533. https://doi.org/10.3390/ma6062522
Chicago/Turabian StyleDhingra, Shruti, Masamune Morita, Tsuyoshi Yoda, Mun'delanji C. Vestergaard, Tsutomu Hamada, and Masahiro Takagi. 2013. "Dynamic Morphological Changes Induced By GM1 and Protein Interactions on the Surface of Cell-Sized Liposomes" Materials 6, no. 6: 2522-2533. https://doi.org/10.3390/ma6062522