First Report of Entomopathogenic Fungi Occurrence in Forest Soils in Croatia
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling Sites and Collection of Soil Samples
2.2. Isolation of Fungi
2.3. Identification of Fungal Species
2.4. Statistical Analysis
3. Results
4. Discussion
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sampling Sites | Coordinates | Time of Collection of Insects | Dominant Tree Species | Insect Host | Development Stage | Identified Fungus | Soil (Type, pH, Moisture) | Time of Collection of Soil |
---|---|---|---|---|---|---|---|---|
Skradin | 43°49′08.1″ N 15°53’57.7″ E | October/ 2014 | Pinus halepensis | Dendrolimus pini | larvae | Beauveria bassiana | Lithosol pH 8.4 very dry | June/2019 (summer) |
Požega (Milan Lug) | 45°23′47.8″ N 17°58′37.4″ E | January/ 2017 | Quercus sp. Fagus sylvatica | Calliteara pudibunda | larvae | Beauveria bassiana | Colluvial pH 7.7 semidry | March/2020 (spring) |
Mrkopalj (Sungerski Lug) | 45°19′57.9″ N 14°47′31.6″ E | February/ 2017 | Picea abies | Ips typographus | adults | Beauveria bassiana | Dystric cambisol pH 4.9 semidry | July/2019 (summer) |
Dugi otok (Nature Park Telašćica) | 43°53′19.4″ N 15°09′41.9″ E | September/ 2017 | Pinus halepensis | Dendrolimus pini | larvae | Beauveria bassiana | Regosol pH 8.0 dry | November/2018 (autumn) |
Spačva (Management Unit Ceranski lugovi) | 45°10′41.3″ N 18°43′54.9″ E | May/ 2018 | Quercus robur | Corythucha arcuata | adults | Beauveria pseudobassiana | Mollic humogley pH 7.8 very wet | February/2019 (winter) |
Identified Species | Average Number of CFU in 1 g of Soil at Different Sampling Locations (CFU × 103 g−1) | ||||
---|---|---|---|---|---|
Location 1 (Dugi Otok) | Location 2 (Skradin) | Location 3 (Spačva) | Location 4 (Mrkopalj) | Location 5 (Požega) | |
Entomopathogenic fungi | |||||
Beauveria spp. | 2.6 | 5.3 | 4 | 6.2 | - |
Metarhizium spp. | 0.4 | - | - | 11 | 20 |
Purpureocillium spp. | 4.4 | 17 | - | 0.6 | - |
Lecanicillium spp. | 1 | 0.3 | - | - | 1 |
Paecilomyces spp. | - | - | - | - | 6.4 |
∑ | 8.4 | 22.6 | 4 | 17.8 | 27.4 |
Fungi with potential entomopathogenic abilities | |||||
Tolypocladium spp. | - | - | - | 0.2 | - |
Trichosporon spp. | - | - | - | - | 1.6 |
Fungi of unproven entomopathogenic abilities | |||||
Pochonia spp. | 10 | 3.3 | 8.7 | 5.4 | - |
Absidia spp. | 0.08 | - | - | 5 | |
Pseudogymnoascus spp. | - | - | 0.6 | - | - |
∑ | 10.1 | 3.3 | 9.3 | 5.6 | 6.6 |
Total ∑ | 18.5 | 25.9 | 13.3 | 23.4 | 34 |
Scheme | SS | Df | MS | F | p-Value | F Crit |
---|---|---|---|---|---|---|
Entomopathogenic fungi | 510.3603 | 4 | 127.5901 | 5.027412 | 0.000984 | 2.462615 |
Locality | 335.4643 | 4 | 83.86608 | 3.304562 | 0.013775 | 2.462615 |
Entomopathogenic fungi*Locality | 2015.057 | 16 | 125.9411 | 4.962436 | 2.15 × 10−7 | 1.745647 |
Error | 2537.888 | 100 | 25.37888 | |||
Total | 5398.77 | 124 |
Comparison | Absolute Difference | Critical Value |
---|---|---|
Beauveria vs. Lecanicillium | 2.72 | 3.6303 |
Beauveria vs. Metarhizium | 3.12 | 3.6303 |
Beauveria vs. Paecilomyces | 1.28 | 3.6303 |
Beauveria vs. Purpureocillium | 3.096 | 3.6303 |
Lecanicillium vs. Metarhizium | 5.84 * | 3.6303 |
Lecanicillium vs. Paecilomyces | 0.76 | 3.6303 |
Lecanicillium vs. Purpureocillium | 2.576 | 3.6303 |
Metarhizium vs. Paecilomyces | 5.08 * | 3.6303 |
Metarhizium vs. Purpureocillium | 3.264 | 3.6303 |
Paecilomyces vs. Purpureocillium | 1.816 | 3.6303 |
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Kovač, M.; Tkaczuk, C.; Pernek, M. First Report of Entomopathogenic Fungi Occurrence in Forest Soils in Croatia. Forests 2021, 12, 1690. https://doi.org/10.3390/f12121690
Kovač M, Tkaczuk C, Pernek M. First Report of Entomopathogenic Fungi Occurrence in Forest Soils in Croatia. Forests. 2021; 12(12):1690. https://doi.org/10.3390/f12121690
Chicago/Turabian StyleKovač, Marta, Cezary Tkaczuk, and Milan Pernek. 2021. "First Report of Entomopathogenic Fungi Occurrence in Forest Soils in Croatia" Forests 12, no. 12: 1690. https://doi.org/10.3390/f12121690