NMR-Metabolic Methodology in the Study of GM Foods
Abstract
:Abbreviations
1. Introduction
2. Results and Discussion
2.1. Sample Production
2.2. Sampling
2.3. Sample Preparation for the NMR Analysis
2.4. Acquisition of NMR Spectra
2.5. NMR - Metabolic Application: Characterization of Transgenic and Conventional Lettuce
3. Experimental Section
3.1. Plant Material and Sampling
3.2. NMR Analysis
3.3. Measurement of the Metabolic Content in Aqueous Extract
3.4. Statistical Analysis
4. Conclusions
Acknowledgements
References
- Fiehn, O. Metabolomics - the link between genotypes and phenotypes. Plant Mol. Biol. 2002, 48, 155–171. [Google Scholar]
- Fiehn, O.; Kopka, J.; Dörmann, P.; Altmann, T.; Trethewey, R.N.; Willmitzer, L. Metabolite profiling for plant functional genomics. Nature Biotechnol. 2000, 18, 1157–1161. [Google Scholar]
- Fan, T.W.-M. Metabolite profiling by one- and two-dimensional NMR analysis of complex mixtures. Prog. Nucl. Magn. Reson. Spectrosc. 1996, 28, 161–219. [Google Scholar]
- Mannina, L.; Segre, A.L. High resolution NMR: from chemical structure to food authenticity. Grasas Aceites 2002, 53, 22–33. [Google Scholar]
- Sobolev, A.P.; Segre, A.L.; Lamanna, R. Proton high-field NMR study of tomato juice. Magn. Reson. Chem. 2003, 41, 237–245. [Google Scholar]
- Sobolev, A.P.; Brosio, E.; Gianferri, R.; Segre, A.L. Metabolic profile of lettuce leaves by high-field NMR spectra. Magn. Reson. Chem. 2005, 43, 625–638. [Google Scholar]
- Mannina, L.; Sobolev, A.P.; Capitani, D.; Iaffaldano, N.; Rosato, M.P.; Ragni, P.; Reale, A.; Sorrentino, E.; D'Amico, I.; Coppola, R. NMR metabolic profiling of organic and aqueous sea bass extracts: Implications in the discrimination of wild and cultured sea bass. Talanta 2008, 77, 433–444. [Google Scholar]
- Sobolev, A.P.; Segre, A.L.; Giannino, D.; Mariotti, D.; Nicolodi, C.; Brosio, E.; Amato, M.E. Strong increase of foliar inulin occurs in transgenic lettuce plants (Lactuca sativa L.) overexpressing the Asparagine Synthetase A gene from Escherichia coli. J. Agric. Food. Chem. 2007, 55, 10827–10831. [Google Scholar] [PubMed]
- Mattoo, A.K.; Sobolev, A.P.; Neelam, A.; Goyal, R.K.; Handa, A.K.; Segre, A.L. NMR spectroscopy based metabolite profiling of transgenic tomato fruit engineered to accumulate spermidine and spermine reveals enhanced anabolic and nitrogen-carbon interactions. Plant Physiol. 2006, 142, 1759–1770. [Google Scholar]
- Piccioni, F.; Capitani, D.; Zolla, L.; Mannina, L. NMR metabolic profiling of transgenic maize with the Cry1A(b) gene. J. Agric. Food. Chem. 2009, 57, 6041–6049. [Google Scholar]
- Baker, J.M.; Hawkins, N.D.; Ward, J.L.; Lovegrove, A.; Napier, J.A.; Shewry, P.R.; Beale, M.H. A metabolomic study of substantial equivalence of field-grown genetically modified wheat. Plant Biotech. J. 2006, 4, 381–392. [Google Scholar]
- Charlton, A.; Allnutt, T.; Holmes, S.; Chisholm, J.; Bean, S.; Ellis, N.; Mullineaux, P.; Oehlschlager, S. NMR profiling of transgenic peas. Plant Biotech. J. 2004, 2, 27–35. [Google Scholar]
- Frugis, G.; Giannino, D.; Mele, G.; Nicolodi, C.; Chiappetta, A.; Bitonti, M.B.; Innocenti, A.M.; Dewitte, W.; Van Onckelen, H.; Mariotti, D. Overexpression of KNAT1 in lettuce shifts leaf determinate growth to a shoot-like indeterminate growth associated with an accumulation of isopentenyl-type cytokinins. Plant Physiol. 2001, 126, 1370–1380. [Google Scholar]
- Giannino, D.; Nicolodi, C.; Testone, G.; Di Giacomo, E.; Iannelli, M.A.; Frugis, G.; Mariotti, D. Pollen-mediated transgene flow in lettuce (Lactuca sativa L.). Plant Breed. 2008, 127, 308–314. [Google Scholar]
- Mou, B. Lettuce. In Vegetables I: Asteraceae, Brassicaceae, Chenopodicaceae, and Cucurbitaceae; Nuez, F., Ed.; Springer: New York, NY, USA, 2008; pp. 75–116. [Google Scholar]
- Neelam, A.; Cassol, T.; Mehta, R.A.; Abdul-Baki, A.A.; Sobolev, A.P.; Goyal, R.K.; Abbott, J.; Segre, A.L.; Handa, A.K.; Mattoo, A.K. A field-grown transgenic tomato line expressing higher levels of polyamines reveals legume cover crop mulch-specific perturbations in fruit phenotype at the levels of metabolite profiles, gene expression, and agronomic characteristic. J. Exp. Botany 2008, 59, 2337–2346. [Google Scholar]
- Gil, A.M.; Duarte, I.F.; Delgadillo, I.; Colquhoun, I.J.; Casuscelli, F.; Humpfer, E.; Spraul, M. Study of the compositional changes of mango during ripening by use of Nuclear Magnetic Resonance spectroscopy. J. Agric. Food. Chem. 2000, 48, 1524–1536. [Google Scholar]
- Bligh, E.G.; Dyer, W.J. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 1959, 37, 911–917. [Google Scholar]
- Voehler, M.W.; Collier, G.; Young, J.K.; Stone, M.P.; Germann, M.W. Performance of cryogenic probes as a function of ionic strength and sample tube geometry. J. Magn. Reson. 2006, 183, 102–109. [Google Scholar]
- Jacobsen, N.E. NMR spectroscopy explained: simplified theory, applications and examples for organic chemistry and structural biology; John Wiley & Sons Inc.: Hoboken, NJ, USA, 2007; pp. 109–110. [Google Scholar]
- Box, G.E.P.; Hunter, J.S.; Hunter, W.G. Factorial design at two levels. In Statistics for experimenters: design, discovery and innovation., 2; Box G.E.P.;, Hunter, J.S.; Hunter, W.G., Eds.; John Wiley & Sons Inc.: Hoboken, NJ, USA, 2005; pp. 173–222. [Google Scholar]
- Mele, G.; Ori, N.; Sato, Y.; Hake, S. The knotted1-like homeobox gene BREVIPEDICELLUS regulates cell differentiation by modulating metabolic pathways. Genes Develop. 2003, 17, 2088–2093. [Google Scholar]
- Frugis, G.; Giannino, D.; Nicolodi, C.; Innocenti, A.M.; Mariotti, D. Lettuce plants that overexpress the KNAT1 homeotic gene from Arabidopsis thaliana exhibit alteration in bolting and flower differentiation. Flower. Newslett. 2001, 32, 16–21. [Google Scholar]
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Sobolev, A.P.; Capitani, D.; Giannino, D.; Nicolodi, C.; Testone, G.; Santoro, F.; Frugis, G.; Iannelli, M.A.; Mattoo, A.K.; Brosio, E.; et al. NMR-Metabolic Methodology in the Study of GM Foods. Nutrients 2010, 2, 1-15. https://doi.org/10.3390/nu2010001
Sobolev AP, Capitani D, Giannino D, Nicolodi C, Testone G, Santoro F, Frugis G, Iannelli MA, Mattoo AK, Brosio E, et al. NMR-Metabolic Methodology in the Study of GM Foods. Nutrients. 2010; 2(1):1-15. https://doi.org/10.3390/nu2010001
Chicago/Turabian StyleSobolev, Anatoly P., Donatella Capitani, Donato Giannino, Chiara Nicolodi, Giulio Testone, Flavio Santoro, Giovanna Frugis, Maria A. Iannelli, Autar K. Mattoo, Elvino Brosio, and et al. 2010. "NMR-Metabolic Methodology in the Study of GM Foods" Nutrients 2, no. 1: 1-15. https://doi.org/10.3390/nu2010001