The Gene vepN Regulated by Global Regulatory Factor veA That Affects Aflatoxin Production, Morphological Development and Pathogenicity in Aspergillus flavus
Abstract
:1. Introduction
2. Results
2.1. Analysis of CHIP-Seq Experimental Results
2.2. Identification A. flavus NRRL3357 Gene vepN
2.3. Construction of the Deletion Mutant (ΔvepN) and Overexpression (OE::vepN) Mutant
2.4. Effects of vepN on Morphology
2.5. Effects of vepN on Conidial Formation
2.6. Effects of vepN on Development of Sclerotia
2.7. Effects of vepN on AFB
2.8. vepN Is Necessary for A. flavus Peanut Infections
2.9. Responses to Different Stressors
2.10. Effects of vepN on the Cell Wall
3. Discussion
4. Materials and Methods
4.1. Strains, Media, and Growth Conditions
4.2. CHIP-Seq Experiment
4.3. Phylogenetic Analysis
4.4. Construction of Gene Knockout and Overexpression Strains
4.5. Observation of Conidia Morphology and Colonies
4.6. Conidial Formation
4.7. Sclerotial Production
4.8. Determination of Aflatoxin
4.9. Seed Infection
4.10. Stress Experiment
4.11. Transmission Electron Microscope (TEM)
4.12. RT-qPCR Analysis
4.13. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strains | Description |
---|---|
WT | A. flavus NRRL3357 wild type |
TJES19.1 | ΔKu70 and ΔpyrG |
ΔvepN | ΔKu70, ΔvepN, and pyrG+ |
OE::vepN | ΔKu70, ΔpyrG, vepN+, gpdA+, and SH ble+ |
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Xu, J.; Jiang, M.; Wang, P.; Kong, Q. The Gene vepN Regulated by Global Regulatory Factor veA That Affects Aflatoxin Production, Morphological Development and Pathogenicity in Aspergillus flavus. Toxins 2024, 16, 174. https://doi.org/10.3390/toxins16040174
Xu J, Jiang M, Wang P, Kong Q. The Gene vepN Regulated by Global Regulatory Factor veA That Affects Aflatoxin Production, Morphological Development and Pathogenicity in Aspergillus flavus. Toxins. 2024; 16(4):174. https://doi.org/10.3390/toxins16040174
Chicago/Turabian StyleXu, Jia, Mengqi Jiang, Peng Wang, and Qing Kong. 2024. "The Gene vepN Regulated by Global Regulatory Factor veA That Affects Aflatoxin Production, Morphological Development and Pathogenicity in Aspergillus flavus" Toxins 16, no. 4: 174. https://doi.org/10.3390/toxins16040174