Edodin: A New Type of Toxin from Shiitake Mushroom (Lentinula edodes) That Inactivates Mammalian Ribosomes
Abstract
:1. Introduction
2. Results
2.1. Isolation of Edodin
2.2. Enzymatic Activity of Edodin
2.3. Cytotoxicity of Edodin and Antifungal Activity Assay
2.4. Edodin Sequence
2.5. Prediction of Edodin Structure
2.6. Putative Active Site of Edodin
2.7. Homology of Edodin with Other Hypothetical Proteins
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Reagents, Cells, and Fungi
5.2. Methods
5.2.1. Purification of Edodin
5.2.2. Analytical Procedures
5.2.3. Cell-Free Protein Synthesis and Enzyme Activity Assays
5.2.4. Cell Viability and Antifungal Activity Assays
5.2.5. cDNA Synthesis, Cloning, and Sequencing
5.2.6. Edodin Cleavage and MALDI-ToF MS Analysis
5.2.7. Prediction of Edodin Structure
5.2.8. Sequence Alignment and Phylogenetic Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Citores, L.; Ragucci, S.; Gay, C.C.; Russo, R.; Chambery, A.; Di Maro, A.; Iglesias, R.; Ferreras, J.M. Edodin: A New Type of Toxin from Shiitake Mushroom (Lentinula edodes) That Inactivates Mammalian Ribosomes. Toxins 2024, 16, 185. https://doi.org/10.3390/toxins16040185
Citores L, Ragucci S, Gay CC, Russo R, Chambery A, Di Maro A, Iglesias R, Ferreras JM. Edodin: A New Type of Toxin from Shiitake Mushroom (Lentinula edodes) That Inactivates Mammalian Ribosomes. Toxins. 2024; 16(4):185. https://doi.org/10.3390/toxins16040185
Chicago/Turabian StyleCitores, Lucía, Sara Ragucci, Claudia C. Gay, Rosita Russo, Angela Chambery, Antimo Di Maro, Rosario Iglesias, and José M. Ferreras. 2024. "Edodin: A New Type of Toxin from Shiitake Mushroom (Lentinula edodes) That Inactivates Mammalian Ribosomes" Toxins 16, no. 4: 185. https://doi.org/10.3390/toxins16040185