Combination of CTec2 and GH5 or GH26 Endo-Mannanases for Effective Lignocellulosic Biomass Degradation

Round 1

Reviewer 1 Report

In this publication, application of combination of CTec2 and GH5 or GH26 endo-mannanase in lignocellulosic biomass degradation is described. This publication seems to be within the scope of journal. However it needs several corrections to be more acceptable for publication.

1. The title of the publication is too long and does not fully represent the content of the publication. In my opinion, the most important discovery in terms of application is the effectiveness of the combination of commercial CTec2 and endo-mannases. I suggest changing the title to e.g. “Combination of CTec2 and GH5 or GH26 endo-mannanase for lignocellulosic biomass degradation”.
2. In abstract please add information that CTec2 is commercial cellulolytic preparation.
3. In introduction please add short characteristics of plant material used in present work. Information about differences in chemical structure between this materials is also very important.
4. In line 52 appropriate references is needed.
5. At the beginning of the results and discussion section, a brief explanation is missing, what prompted researchers to choose CTec2 as a commercial preparation. Have other commercial preparations been tested? Please add short description of chemical structure of chosen model cellulosic substrates and explanation, why these cellulosic substrates were chosen for the tests and not others.
6. All used abbreviations should be explained when they appear for the first time in the text.
7. Information about thermo-stability of enzymes are very important for practical application and must be detailed described in manuscript.
8. Analytical methods description is absolutely insufficient. Estimation of glucose, mannose and xylose is crucial in this work. DNS assay also should be detailed described.

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

Referee report catalysts (GH5 GH26)

This is a paper describing the action and conversion of glycoside hydrolase (GH) family 5 (GH5) and Gh26 beta-mannases, which are industrially important enzymes (hemicellulases) that participate in the hydrolysis of lignocellulose substrates.

The paper is, interesting with a good concise discussion and easy follow and well prepared. I have juts rather few comments for the authors to consider and for potential improvement.

General comments.

It could be woth to mention the crystallinity of the lignocellulosic materials used (in general, from potential literature values) if this is known.

Specific comments

Abstract: line 16 instead of “on mannan” suggest writing “using mannan substrates”

Table 1: no unit is given

L65 (and potentially elsewhere) suggest write “negative-numbered binding subsites” (to avoid misunderstanding that it would be a negitve charge)

L72 suggest write “of at least some GH26”

Figure 1. Would be good with error bars

L92 “ware” should be “are” I assume

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

The authors carefully revised the manuscript. Due to this fact, the article in its current form can be accepted for publication.