Co-Immobilization of Superoxide Dismutase with Catalase on Soft Microparticles Formed by Self-Assembly of Amphiphilic Poly(Aspartic Acid)
Abstract
:1. Introduction
2. Results and Discussion
2.1. Purification of the Fusion Enzyme
2.2. Immobilization of SOD-ELP and CAT-ELP on HPASP
2.3. Secondary Structures of Enzymes Monitored by Circular Dichroism Spectra
2.4. Stability of the Immobilized Enzymes against Denaturing by Urea
2.5. Enzymatic Activity
3. Experimental Section
3.1. Materials
3.2. Protein Expression and Purification
3.3. Co-Immobilization of SOD-ELP and CAT-ELP on HPASP
3.4. Characterization
3.5. Fluorescence Measurements
3.6. Enzyme Activity Analysis
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Mao, S.; Li, R.; Wang, W.; Feng, W.; Ji, P. Co-Immobilization of Superoxide Dismutase with Catalase on Soft Microparticles Formed by Self-Assembly of Amphiphilic Poly(Aspartic Acid). Catalysts 2017, 7, 217. https://doi.org/10.3390/catal7070217
Mao S, Li R, Wang W, Feng W, Ji P. Co-Immobilization of Superoxide Dismutase with Catalase on Soft Microparticles Formed by Self-Assembly of Amphiphilic Poly(Aspartic Acid). Catalysts. 2017; 7(7):217. https://doi.org/10.3390/catal7070217
Chicago/Turabian StyleMao, Siyu, Rong Li, Wenchen Wang, Wei Feng, and Peijun Ji. 2017. "Co-Immobilization of Superoxide Dismutase with Catalase on Soft Microparticles Formed by Self-Assembly of Amphiphilic Poly(Aspartic Acid)" Catalysts 7, no. 7: 217. https://doi.org/10.3390/catal7070217