Design, Synthesis, and Characterization of Schiff Base Bond-Linked pH-Responsive Doxorubicin Prodrug Based on Functionalized mPEG-PCL for Targeted Cancer Therapy
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials
2.2. Synthesis of mPEG-PCL with Substituted Carboxylic Groups (mPEG-CPCL)
2.2.1. Synthesis of mPEG-BuPCL Block Copolymer
2.2.2. Synthesis of mPEG-CPCL
2.3. Synthesis of Imine-Linked DOX Prodrugs Based on PEG-PCL di-Block Copolymer (mPEG-PCL-Imi-DOX)
2.3.1. Synthesis of Aldehyde-Functionalized mPEG-PCL (mPEG-APCL)
2.3.2. Synthesis of mPEG-PCL-Imi-DOX
2.4. Structural Characterization
2.5. Preparation and Characterization of mPEG-PCL-Imi-DOX Micelles
2.6. In Vitro Drug Release
2.7. In Vitro Cellular Uptake
2.8. In Vitro Cytotoxicity Assay
3. Results and Discussion
3.1. Polymer Synthesis and Characterization
3.2. Characterization of mPEG-PCL-Imi-DOX Micelles
3.3. In Vitro Drug Release of mPEG-PCL-Imi-DOX Micelles
3.4. In Vitro Cellular Uptake and Cytotoxicity Studies of mPEG-PCL-Imi-DOX Micelles
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Zhai, Y.; Zhou, X.; Zhang, Z.; Zhang, L.; Wang, D.; Wang, X.; Sun, W. Design, Synthesis, and Characterization of Schiff Base Bond-Linked pH-Responsive Doxorubicin Prodrug Based on Functionalized mPEG-PCL for Targeted Cancer Therapy. Polymers 2018, 10, 1127. https://doi.org/10.3390/polym10101127
Zhai Y, Zhou X, Zhang Z, Zhang L, Wang D, Wang X, Sun W. Design, Synthesis, and Characterization of Schiff Base Bond-Linked pH-Responsive Doxorubicin Prodrug Based on Functionalized mPEG-PCL for Targeted Cancer Therapy. Polymers. 2018; 10(10):1127. https://doi.org/10.3390/polym10101127
Chicago/Turabian StyleZhai, Yinglei, Xing Zhou, Zhiqiang Zhang, Lei Zhang, Dianyu Wang, Xinhui Wang, and Wei Sun. 2018. "Design, Synthesis, and Characterization of Schiff Base Bond-Linked pH-Responsive Doxorubicin Prodrug Based on Functionalized mPEG-PCL for Targeted Cancer Therapy" Polymers 10, no. 10: 1127. https://doi.org/10.3390/polym10101127